Nlk, adk, adirondack, Nemo-like kinase
protein serine/threonine kinase involved in establishing ommatidial rotation/planar cell polarity, retinal specification and neuronal function, and circadian clock speed - regulates the activity of several signal transduction pathways, including BMP, Wnt, and Notch - implicated in fly models of neurodegenerative diseases
Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Stop-codon suppression (UGA) postulated; FBrf0234051.
Gene model reviewed during 6.25
7.6, 7.2, 5.8, 5.4, 4.6, 4.1, 3.6, 3.2 (northern blot)
477, 434 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nmo using the Feature Mapper tool.
Isoforms of nmo with extended 3' UTRs appear later in embryogenesis. Sequential, phased, 3' UTR lengthening is seen (3' UTR lengthening ocurring in multiple steps during later stages of embryogenesis).
One nmo isoform is expressed in a stripe posterior the morphogenetic furrow. The other is expressed at low levels throughout the disc.
nmo transcripts are expressed in a complex pattern throughout development. Six transcripts are expressed in early embryos, (3.2, 3.6, 4.1, 4.6, 5.4, and 5.8 kb). A 7.2kb transcript appears in late embryos and a 7.6kb transcript is detected in adult heads. The seven transcripts are all developmentally regulated with varying patterns. In general, transcription is highest in embryos, third instar larvae, and pupae.
GBrowse - Visual display of RNA-Seq signalsView Dmel\nmo in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
nmo is required in the larval eye disc through most of the first 45[o] of ommatidial rotation to regulate the speed at which the ommatidial precursors move. nmo is required in photoreceptors R1, R6 and R7 for normal orientation.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, spindle abnormalities, centrosome abnormalities, lagging chromatids and a whole range of mitotic abnormalities are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila Nemo gene, Acc. No. U12010.
Area matching Drosophila EST AA439393 (inverted).
"Nemo" is Korean for a four-sided figure.
The nmo gene is required for the rotation of photoreceptor clusters in the developing eye. Mosaic analysis indicates that nmo acts within the ommatidium, suggesting local autonomy of the determination of rotation. nmo+ function in any photoreceptor cell is sufficient for normal rotation.