FlyBase Gene Report: Dmel\p38a

General Information

Symbol

Dmel\p38a

Species

D. melanogaster

Name

p38a MAP kinase

Annotation Symbol

CG5475

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015765

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

mitogen-activated protein kinase (2.7.11.24)

Gene Summary

p38a MAP kinase (p38a) encodes a member of the mitogen-activated protein kinases responsive to diverse stresses. In immune response, it activates its downstream component encoded by Atf-2 that in turn regulates Duox expression. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

p38, Mpk2, D-p38a, Erk2, Dp38

Key Links

Genomic Location

Cytogenetic map

95E5-95E5

Sequence location

3R:24,150,416..24,152,321 [-]

Recombination map

3-83

RefSeq locus

NT_033777 REGION:24150416..24152321

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (28 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables MAP kinase activity

inferred from genetic interaction with SGD:S000004103

(Han et al., 1998)

inferred from direct assay

(Han et al., 1998)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR003527, InterPro:IPR008352, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables MAP kinase activity

inferred from sequence or structural similarity with UniProtKB:Q16539

(Han et al., 1998)

traceable author statement

(Martin-Blanco, 2000)

inferred from electronic annotation with InterPro:IPR003527, InterPro:IPR008352

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

Biological Process (22 terms)

Terms Based on Experimental Evidence (20 terms)

CV Term

Evidence

References

involved_in cellular response to arsenic-containing substance

inferred from direct assay

(Ryabinina et al., 2006)

involved_in cellular response to cadmium ion

inferred from direct assay

(Ryabinina et al., 2006)

involved_in cellular response to reactive oxygen species

inferred from direct assay

(Ryabinina et al., 2006)

involved_in defense response to bacterium

inferred from mutant phenotype

(Chen et al., 2010, Shinzawa et al., 2009)

involved_in defense response to fungus

inferred from mutant phenotype

(Chen et al., 2010)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Vrailas-Mortimer et al., 2011)

involved_in heart morphogenesis

inferred from genetic interaction with FLYBASE:p38b; FB:FBgn0024846

(Lim et al., 2014)

involved_in immune response

inferred from direct assay

(Han et al., 1998)

involved_in mucosal immune response

inferred from mutant phenotype

(Ha et al., 2009)

involved_in negative regulation of antimicrobial humoral response

inferred from mutant phenotype

(Han et al., 1998)

involved_in p38MAPK cascade

inferred from direct assay

(Han et al., 1998)

involved_in paracrine signaling

inferred from genetic interaction with FLYBASE:p38b; FB:FBgn0024846

(Lim et al., 2014)

involved_in positive regulation of cell size

inferred from genetic interaction with FLYBASE:p38b; FB:FBgn0024846

(Cully et al., 2010)

involved_in regulation of adult chitin-containing cuticle pigmentation

inferred from genetic interaction with FLYBASE:Ask1; FB:FBgn0014006

(Sekine et al., 2011)

involved_in regulation of cellular response to oxidative stress

inferred from mutant phenotype

(Vrailas-Mortimer et al., 2011)

involved_in response to heat

inferred from mutant phenotype

(Gonda et al., 2012, Cai et al., 2011, Cully et al., 2010)

involved_in response to hydrogen peroxide

inferred from mutant phenotype

(Cully et al., 2010)

involved_in response to osmotic stress

inferred from direct assay

(Han et al., 1998)

inferred from mutant phenotype

(Cai et al., 2011)

involved_in response to oxidative stress

inferred from mutant phenotype

(Cai et al., 2011)

involved_in response to starvation

inferred from mutant phenotype

(Cai et al., 2011, Cully et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

involved_in MAPK cascade

inferred from sequence or structural similarity with UniProtKB:Q16539

(Han et al., 1998)

non-traceable author statement

(Martin-Blanco, 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Han et al., 1998)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CONVENTIONAL MITOGEN ACTIVATED PROTEIN KINASES

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. (O62618)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

MAP kinase activity

(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.11.24)

(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.11.24)

protein serine/threonine kinase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

CONVENTIONAL MITOGEN ACTIVATED PROTEIN KINASES -: Conventional Mitogen-activated protein kinases (MAPK) are proline-directed protein serine/threonine kinases that are components of the MAPK signaling cascade. MAPKs are activated by dual phosphorylation on Thr and Tyr residues within a conserved Thr-X-Tyr motif located in the activation loop by MAPK kinases. (Adapted from PMID:21372320).

Protein Function (UniProtKB)

Kinase involved in a signal transduction pathway. May down-regulate insect immunity gene expression after prolonged infection.

(UniProt, O62618)

Summary (Interactive Fly)

p38a serine/threonine protein kinase - involved in response stress including heat shock, oxidative stress and starvation - activates its downstream component Atf-2 that in turn regulates Duox expression

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\p38a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O62618)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

p38a-RA

FBtr0084580

NM_057815

1450

366

p38a-RB

FBtr0084581

NM_170126

1634

366

p38a-RC

FBtr0300572

NM_001170240

1474

366

Additional Transcript Data and Comments

Reported size (kB)

1.438 (longest cDNA)

(Han et al., 1998)

1.441 (longest cDNA)

(Han et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

p38a-PA

FBpp0083965

42.3

366

6.84

FBpp0083966

42.3

366

6.84

FBpp0289799

42.3

366

6.84

O62618

NP_001163711

ACZ95005

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

366 aa isoforms: p38a-PA, p38a-PB, p38a-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

366 (aa); 42 (kD predicted)

(Han et al., 1998)

366 (aa)

(Han et al., 1998)

Comments

External Data

Post Translational Modification

Dually phosphorylated on Thr-184 and Tyr-186, which activates the enzyme.

(UniProt, O62618)

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

(UniProt, O62618)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\p38a using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

p38a_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.40

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 2

organism | ubiquitous

(Han et al., 1998)

embryonic stage 13

embryonic primordium of adult hindgut precursor

(Han et al., 1998)

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

p38a transcripts are detected predominantly in preblastoderm embryos by in situ hybridization. In late embryos, a low level of staining is observed in the posterior region. Transcripts are detected at a low level throughout development on northern blots.

(Han et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Han et al., 1998)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\p38a in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\p38a

View exonic expression by tissue for Dmel\p38a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015765

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42866/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015765

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015765

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015765

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5475

Flygut - An atlas of the Drosophila adult midgut

FBgn0015765

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\p38a

Stages(s) 1-3
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of p38a

Transgenic constructs containing regulatory region of p38a

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3R)crb87-4

(Weber et al., 2000, Paricio et al., 1999)

Df(3R)crb-F89-4

(Weber et al., 2000, Paricio et al., 1999, Martin-Blanco and Martinez Arias, 1997)

Df(3R)Exel6198

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(3R)p38a-c^del

(Chen et al., 2010)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal

p38a^hs.PH

partially lethal - majority die | nutrition conditional

p38a¹

viable

p38a¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

p38a^GD10641

viable, with Scer\GAL4^pnr-MD237

p38a^GD10641

viable, with Scer\GAL4^tin.CΔ4

p38a^GD17018

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

p38a^GL00131

fertile

p38a¹

Other Phenotypes

Allele

abnormal heat stress response

p38a¹

abnormal heat stress response | adult stage

p38a¹

abnormal heat stress response | recessive

p38a¹

abnormal immune response

p38a¹³

abnormal immune response, with Scer\GAL4^da.G32

p38a^RNAi.UAS

abnormal immune response | adult stage

p38a¹

abnormal osmotic stress response

p38a¹

abnormal oxidative stress response

p38a¹

abnormal starvation stress response

p38a¹

abnormal starvation stress response | recessive

p38a¹

abnormal stress response | recessive

p38a¹

non-enhancer of variegation

p38a¹

non-suppressor of variegation

p38a¹

short lived

p38a¹

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (57)

Hsap\MAPK11

12 of 14

Yes

Hsap\MAPK14

12 of 14

Yes

Hsap\MAPK12

7 of 14

Yes

Hsap\MAPK13

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (33)

Rnor\Mapk11

12 of 14

Yes

Rnor\Mapk14

12 of 14

Yes

Rnor\Mapk12

7 of 14

Yes

Rnor\Mapk13

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (32)

Mmus\Mapk11

12 of 14

Yes

Mmus\Mapk14

12 of 14

Yes

Mmus\Mapk12

6 of 14

Yes

Mmus\Mapk13

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (30)

Xtro\mapk11

7 of 13

Yes

Xtro\mapk14

7 of 13

Yes

Xtro\mapk13

5 of 13

Yes

Xtro\XB956263

5 of 13

Yes

4 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (43)

Drer\mapk11

12 of 14

Yes

Drer\mapk14a

12 of 14

Yes

Drer\mapk14b

11 of 14

Drer\mapk12b

7 of 14

Yes

Drer\mapk12a

6 of 14

Yes

Drer\mapk13

6 of 14

Yes

Drer\zgc:171775

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-45c16.2

1 of 14

Drer\si:ch211-220i18.4

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (33)

Cele\pmk-1

13 of 14

Yes

Cele\pmk-2

6 of 14

Yes

Cele\pmk-3

3 of 14

Yes

Cele\F09C12.2

2 of 14

Cele\gska-3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (25)

Agam\AgaP_AGAP012148

6 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (68)

Atha\MPK3

5 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (19)

Scer\HOG1

11 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\sty1

11 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:p38a. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

p38b

13 of 13

p38c

7 of 13

6 of 13

Erk7

5 of 13

bsk

4 of 13

nmo

4 of 13

3 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

mbt

1 of 13

Pak

1 of 13

Pitslre

1 of 13

sgg

1 of 13

Srpk79D

1 of 13

SRPK

1 of 13

wnd

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

colorectal cancer, multigenic, personalized

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAPK11; mitogen-activated protein kinase 11

12 of 14

602898

MAPK14; mitogen-activated protein kinase 14

12 of 14

600289

MAPK12; mitogen-activated protein kinase 12

7 of 14

602399

MAPK13; mitogen-activated protein kinase 13

6 of 14

602899

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

p38a - CG10418

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - CG1092

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - CG2852

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - CG31800

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - CG9372

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - CG9586

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - Hou

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - Jra

enzymatic study, autoradiography

(Han et al., 1998)

p38a - Lam

anti tag coimmunoprecipitation, western blot

(Ryan et al., 2021)

p38a - MAPk-Ak2

experimental knowledge based

(Guruharsha et al., 2011)

p38a - Mecr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - Npl4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - Nup358

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - RfC4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - Tmem214

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - p38b

experimental knowledge based

(Guruharsha et al., 2011)

p38a - ps

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - rad50

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - shi

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38a - stv

anti tag coimmunoprecipitation, western blot

(Ryan et al., 2021)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Ha et al., 2009)

enhanceable

(Seisenbacher et al., 2011)

p38a|p38b

suppressible

Sod2

(Vrailas-Mortimer et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ask1

suppressible

p38a

(Sekine et al., 2011)

suppressible

(Lim et al., 2014)

suppressible

(Mosallanejad et al., 2014)

hiw

suppressible

p38a

(Klinedinst et al., 2013)

p38b

suppressible

p38a

(Seisenbacher et al., 2011)

slim

suppressible

p38a

(Sekine et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67799

DroID - A comprehensive database of gene and protein interactions.

FBgn0015765

MIST (protein-protein) - An integrated Molecular Interaction Database

42866

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O62618

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-83

Cytogenetic map

95E5-95E5

Sequence location

3R:24,150,416..24,152,321 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95E5-95E5

Limits computationally determined from genome sequence between P{PZ}Syx1A⁰⁶⁷³⁷&P{PZ}Syx1A¹⁰⁶⁶⁰ and P{PZ}crb⁰⁷²⁰⁷&P{PZ}BRWD3⁰⁵⁸⁴²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

95E4-95F1

(Han, 1997.11.21)

95E4-95F1

(determined by in situ hybridisation)

(Han et al., 1998)

Experimentally Determined Recombination Data

Location

3-83

(FlyBase, 2016)

3-82.7

(Comeron et al., 2012)

(Martin-Blanco and Martinez Arias, 1997)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

8822

w^*; P{neoFRT}82B p38a¹

27316

y¹ v¹; P{TRiP.JF02625}attP2

602378

y¹ w¹; P{TKO-UAS.GS06186}attP40/CyO

602806

y¹ v¹; P{CFD4FLPOUT-p38a-p38b}attP40/CyO

35244

y¹ sc^* v¹ sev²¹; P{TRiP.GL00131}attP2

FlyORF

F002413

M{UAS-p38a.ORF.3xHA.GW}ZH-86Fb

5475R-1

5475R-2

v102484

v52277

w¹¹¹⁸; P{GD17018}v52277

v34238

w¹¹¹⁸; P{GD10641}v34238/CyO

v341704

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00811}attP40/CyO-GFP

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (31)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH02445

Other clones

DPiM_RH02445

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015765

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015765

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Null mutants are homozygous viable with no developmental defects, though show susceptibility to some environmental stresses including heat shock, oxidative stress and starvation. Immune response profile of Mpk2 mutants is largely normal.

(Craig et al., 2004)

Mpk2 activation in response to NaCl and heat shock is markedly reduced in Mekk1 mutants compared to wild type.

(Inoue et al., 2001)

Mekk1 regulation of the Mpk2/p38b pathway is critical for the response to environmental stresses.

(Inoue et al., 2001)

Mpk2 is cloned and sequenced from a neoplasmic l(2)mbn cell line. Study of the protein and its function reveals that similar osmotic stress-responsive signal transduction pathways are conserved in yeast, Drosophila and mammalian cells.

(Han et al., 1998)

Mpk2 partially functionally complements the function of S.cerevisiae Scer\HOG1 in a hyperosmotic environment.

(Han et al., 1998)

Mpk2 has been cloned and sequenced.

(Han et al., 1998)

Overexpression of Mpk2 suppresses the lipopolysaccharide induction of immunity genes.

(Han et al., 1998)

Relationship to Other Genes

Source for database merge of

Additional comments

The p38a and p38b genes share at least 50% identity.

(Langille and Clark, 2007)

Relationship of "Mpk2" to "Erk-B" not known.

Nomenclature History

Source for database identify of

Source for identity of: Mpk2 CG5475

(Kodira, 1999.11)

Source for identity of: p38a Mpk2

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'Mpk2' to 'p38a' to: i) make nomenclature consistent between p38 genes; ii) reflect the preferred usage in the literature; iii) reflect the preferred symbol used in the first peer-reviewed papers to characterize the gene (FBrf0100059, FBrf0102625).

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (39)

Reported As

Symbol Synonym

CG5475

(Hosono et al., 2015, Chen et al., 2012, Ni et al., 2011.7.19, Vrailas-Mortimer et al., 2011, Zhang et al., 2011, Ni et al., 2010.12.1, Zhang et al., 2010, Keleman et al., 2009.8.5, Morrison et al., 2000)

D-MPK2

(Martin-Blanco, 2000)

D-P38a

(Martin-Blanco, 2000)

D-p38

(Kurz and Ewbank, 2003)

D-p38 MAPK

(Ryabinina et al., 2006)

D-p38a

(Lim et al., 2019, Craig et al., 2004, Craig, 2003.12.16, Inoue et al., 2001, Stronach and Perrimon, 1999, Han et al., 1998)

DMPK2

(Martin-Blanco and Martinez Arias, 1996)

DmMPK2

(Takaesu et al., 1998)

Dmp38a

(Shinzawa et al., 2009)

Dp38

(Sun et al., 2008, Kim et al., 2002, Lee et al., 2000, Han et al., 1998)

Dp38a

(Mosallanejad et al., 2014, Sekine et al., 2012, Sekine et al., 2011)

ERK2

(Murari et al., 2022, Farkas et al., 2009)

Erk2

(Park et al., 2017, Turski et al., 2012, Moses et al., 2011)

MAPK-2

(Abolaji et al., 2015)

MAPK2

(Ecker et al., 2017, Olalekan Abolaji et al., 2014)

MPK2

(Trindade de Paula et al., 2016)

Mpk2

(Engel et al., 2019, Zirin et al., 2015, Gomez et al., 2012, Gonda et al., 2012, Vrailas-Mortimer et al., 2011, Perkins et al., 2009.8.10, Davis et al., 2008, Langille and Clark, 2007, Liu et al., 2007, Sackton et al., 2007, Morozova et al., 2006, Stanyon et al., 2004)

P38

(El Chamy et al., 2015, Sanyal et al., 2005)

dERK2

(Li et al., 2020)

dMPK2

(Martin-Blanco and Martinez Arias, 1997)

dp38

(Shimizu et al., 2008, Sano et al., 2005)

dp38a

(Tian et al., 2014, Shimizu et al., 2008)

group 2

(Biggs and Zipursky, 1992)

mpk2

(Yang et al., 2018, Pereira et al., 2014, Stergiopoulos et al., 2009)

p38 MAPK

(Hegazi et al., 2019, Whon et al., 2017, Lee and Lee, 2014, Demontis et al., 2013)

p38 α

(Bjorklund et al., 2006, Muller et al., 2002)

p38^MAPK

(Zhang and Edgar, 2022)

p38A

(Na et al., 2013, Manning, 2001.10.11)

p38K

(Yu and Hyun, 2021)

p38Ka

(Vrailas-Mortimer et al., 2014, Vrailas-Mortimer et al., 2011)

p38MAPK

(Batista et al., 2016)

p38a

(Alvarez and Papagiannouli, 2025, Braun et al., 2025, He et al., 2025, Wāng, 2025, Fangninou et al., 2024, Fangninou et al., 2023, Gao et al., 2022, Gera et al., 2022, Worley and Hariharan, 2022, Bonfini et al., 2021, Fabian et al., 2021, Scepanovic et al., 2021, Xie et al., 2021, Li et al., 2020, Port et al., 2020, Wang et al., 2020, Bangi et al., 2019, Chai et al., 2019, Liaw and Chiang, 2019, Maier et al., 2019, Sun et al., 2019, Muñoz-Soriano et al., 2018, Jordán-Álvarez et al., 2017, Keesey et al., 2017, Kenmoku et al., 2017, Terriente-Félix et al., 2017, Transgenic RNAi Project members, 2017-, Ashton-Beaucage et al., 2016, Lee et al., 2016, Schimizzi et al., 2016, Santabárbara-Ruiz et al., 2015, Belozerov et al., 2014, Chakrabarti et al., 2014, Dusik et al., 2014, Lim et al., 2014, Sopko et al., 2014, Klinedinst et al., 2013, Schoborg et al., 2013, Gonda et al., 2012, Igboin et al., 2012, Cai et al., 2011, Mathew et al., 2011, Seisenbacher et al., 2011, Seong et al., 2011, Chen et al., 2010, Chen et al., 2010, Cully et al., 2010, Ha et al., 2009, Ha et al., 2009, Davis et al., 2008, Shimizu et al., 2008, Balakireva et al., 2006, Collins et al., 2006, Hsiao et al., 2001, Tootle and Rebay, 2001, Lemaitre, 2000.12.20, Morrison et al., 2000, Weber et al., 2000)

p38α

(Pereira et al., 2014)

Name Synonyms

Erk-kinase-like

Mpk2

p38 MAP kinase

(Sinenko et al., 2021, Balakireva et al., 2007, Harrison et al., 2006)

p38 mitogen-activated protein kinase

(Di Cara et al., 2013, Balakireva et al., 2006)

p38a MAP kinase

(Han, 1997.11.21, Han, 1997.11.21)

stress-activated kinase P38

(Martin-Blanco et al., 1998)

Secondary FlyBase IDs

FBgn0013952
FBgn0022991

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 76 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42866

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O62618

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O62618

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JIV6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O62618

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015765

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42866/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015765

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015765

FlyMine - An integrated database for Drosophila genomics

FBgn0015765

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5475

MARRVEL_MODEL - MARRVEL (model organism gene)

42866

Linkouts

BioGRID - A database of protein and genetic interactions.

67799

Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015765

DroID - A comprehensive database of gene and protein interactions.

FBgn0015765

DRSC - Results frm RNAi screens

FBgn0015765

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

p38a_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015765

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015765

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5475

Flygut - An atlas of the Drosophila adult midgut

FBgn0015765

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0015765

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013594

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

42866

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O62618

References (225)

The following external sites may use different assemblies or annotations than FlyBase.

-0.40

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