- Tools
- Downloads
- Links
- Community
- About
- Help
FB2026_01
,
released March 12, 2026
CK2, CK2α, CKII, Tik, casein kinase 2
CkII catalytic and regulatory subunits - modulates Antennapedia's properties, restricting its activity to an appropriate level - a regulator of the active zone protein Bruchpilot - stabilizes Clock and represses its activity in circadian oscillator - promotes Hedgehog signaling by regulating both smoothened and Cubitus interrupt Myc and Casein kinase 2 target mushroom body miniature, which is required for ribosome biogenesis and cell growth of central brain neuroblasts
Please see the JBrowse view of Dmel\CkIIα for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.55
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
1.467 (longest cDNA)
336 (aa); 39.833 (kD predicted)
36.7 (kD observed)
Purified CkII protein kinases were able to phosphorylate acid-soluble proteins from Drosophila Kc cells.
Tetramer of two alpha and two beta chains.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CkIIα using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: 3 hours after ZT
JBrowse - Visual display of RNA-Seq signals
View Dmel\CkIIα in JBrowse3-47
3-44.0
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, centrosome abnormalities and lagging chromatids are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
A dominant mutant, which has a long period phenotype as a heterozygote. Homozygotes die during the third larval instar stage. The recessive lethal is not separable from the circadian phenotype, suggesting a vital role for the CkIIα gene.
Identification: screen for circadian locomotor behavior mutants.
Casein kinase II specifically phosphorylates a set of serine residues within the cact PEST domain.
Casein kinase II phosphorylates Ser468 (a residue in the PEST domain) of cact in vitro.
Recombinant CkII in vitro binds to spermine and this interaction is concomitant with a striking effect on the structural polymeric organisation on the kinase which in the presence of the polyamine exhibits a ring-like structure.
Casein kinase II reconstituted from heterologous subunits displays properties similar to those of the homologous protein.
Casein kinase II-mediated phosphorylation of Top2 stimulates its activity by enhancing the ability of the enzyme to hydrolyse its high energy ATP cofactor.
S.cerevisiae Scer\cka1 Scer\cka2 double mutants are functionally complemented by CkIIα.
Isolation and sequencing of cDNA clones encoding both subunits of casein kinase II.
Source for merge of: CkIIα Tik
Source for merge of: CkIIα anon-WO02059370.53
Source for merge of: CkIIα l(3)S095808
Complementation tests and recombinational mapping suggest the CkIIα mutant maps to a novel circadian rhythm gene.
Source for merge of CkIIα anon-WO02059370.53 was sequence comparison ( date:051113 ).
Drosophila Casein kinase II α and β subunits can rescue the lethality of S.cerevisiae deleted for both genes.
