FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(spl)m8-HLH, CkIIα
FlyBase ID
FBig0000108586
Interaction Network
Interactions Browser links
E(spl)m8-HLH network
CkIIα network
Reported Interactions
FBrf0174882-2.Y2H
Reference
Karandikar et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(spl)m8-HLH 

M8

bait

fused to lexA DNA-binding domain

E(spl)m8-HLH 
E(spl)m8-HLH 
E(spl)m8-HLH 
CkIIα 

CK2α

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(spl)m8-HLH 
C-terminal domain
necessary binding region
aa 124-179

coordinates relative to E(spl)m8-HLH-PA

E(spl)m8-HLH 
CK2 domain residue 159
mutation disrupting interaction
aa 159

S159D, coordinates relative to E(spl)m8-HLH-PA

E(spl)m8-HLH 
CK2 domain residue 159
mutation decreasing interaction
aa 159

S159A, coordinates relative to E(spl)m8-HLH-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Karandikar et al., 2004)

Two-hybrid system: yeast lexA-BD/VP16-AD

(Karandikar et al., 2004)
FBrf0216468-1.Y2H
Reference
Eastwood et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(spl)m8-HLH 

E(spl)m8-HLH

bait

fused to GAL4 DNA-binding domain

E(spl)m8-HLH 
E(spl)m8-HLH 
CkIIα 

CkIIα

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(spl)m8-HLH 
CtD domain
sufficient binding region
aa 123-179
E(spl)m8-HLH 
phosphorylation domain
mutation disrupting interaction
aa 159-175
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Eastwood et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Eastwood et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)