Prey derived from embryonic cDNA library.
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Bait expressed and purified from bacteria.
Prey expressed and purified from yeast.
Purified proteins were mixed and potential complexes were detected by glycerol gradient centrifugation and detection of kinase activity.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
HGScore = 82.64174
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)
MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)
MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
