FB2026_03 , released September 17, 2026
FB2026_03 , released September 17, 2026
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Term affinity chromatography technology ID (Ontology) MI:0004 (Molecular Interactions)
Definition This class of approaches is characterised by the use of affinity resins as tools to purify molecule of interest (baits) and their binding partners. The baits can be captured by a variety of high affinity ligands linked to a resin - for example, antibodies specific for the bait itself, antibodies for specific tags engineered to be expressed as part of the bait or other high affinity binders such as glutathione resins for GST fusion proteins, metal resins for histidine-tagged proteins.[ PubMed:7708014 ]
Also Known As "affinity chrom" ; "Affinity purification"
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biochemical
 |__chromatography technology__
 |__affinity technology________|
                               affinity chromatography technology  13331 rec.
                                |__coimmunoprecipitation 11530 rec.
                                |   |__anti bait coimmunoprecipitation 3197 rec.
                                |   |__anti tag coimmunoprecipitation 9305 rec.
                                |   |__chromatin immunoprecipitation assay(+)
                                |   |__immunodepleted coimmunoprecipitation 1 rec.
                                |   |__miniaturized immunoprecipitation
                                |   |__rna immunoprecipitation
                                |__luminescence based mammalian interactome mapping 6 rec.
                                |__pull down 3066 rec.
                                |   |__interactome parallel affinity capture
                                |__tandem affinity purification 144 rec.
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Is a chromatography technology
affinity technology
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  • "affinity chrom" EXACT PSI-MI-short
    "Affinity purification" EXACT PSI-MI-alternate
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