FlyBase Gene Report: Dmel\p38b

General Information

Symbol

Dmel\p38b

Species

D. melanogaster

Name

p38b MAP kinase

Annotation Symbol

CG7393

Feature Type

protein_coding_gene

FlyBase ID

FBgn0024846

Gene Model Status

Current

Stock Availability

22 publicly available

Enzyme Name (EC)

mitogen-activated protein kinase (2.7.11.24)

Gene Summary

p38b MAP kinase (p38b) encodes the serine/threonine kinase that constitutes MAP kinase signaling cascades. It is phosphorylated by MAPK kinases and phosphorylates various substrates that play roles in stress responses, immune responses and differentiation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

p38, D-p38b, p38β, p38Kb, Dp38

Key Links

Genomic Location

Cytogenetic map

34D1-34D1

Sequence location

2L:13,780,689..13,782,611 [+]

Recombination map

2-49

RefSeq locus

NT_033779 REGION:13780689..13782611

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (34 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables MAP kinase activity

inferred from direct assay

(Huang et al., 2016, Han et al., 1998)

enables protein serine/threonine kinase activity

inferred from direct assay

(Belozerov et al., 2014)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR003527, InterPro:IPR008352, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables MAP kinase activity

non-traceable author statement

(Martin-Blanco, 2000)

inferred from electronic annotation with InterPro:IPR003527, InterPro:IPR008352

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

non-traceable author statement

(Adachi-Yamada et al., 1999)

Biological Process (28 terms)

Terms Based on Experimental Evidence (26 terms)

CV Term

Evidence

References

involved_in cellular response to arsenic-containing substance

inferred from direct assay

(Ryabinina et al., 2006)

involved_in cellular response to cadmium ion

inferred from direct assay

(Ryabinina et al., 2006)

involved_in cellular response to reactive oxygen species

inferred from direct assay

(Ryabinina et al., 2006)

involved_in circadian rhythm

inferred from mutant phenotype

(Vrailas-Mortimer et al., 2014)

involved_in defense response to bacterium

inferred from mutant phenotype

(Chen et al., 2010, Shinzawa et al., 2009)

involved_in defense response to fungus

inferred from mutant phenotype

(Chen et al., 2010)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Vrailas-Mortimer et al., 2011)

involved_in heart morphogenesis

inferred from genetic interaction with FLYBASE:p38a; FB:FBgn0015765

(Lim et al., 2014)

involved_in imaginal disc-derived wing morphogenesis

inferred from direct assay

(Adachi-Yamada et al., 1999)

involved_in immune response

inferred from mutant phenotype

(Han et al., 1998)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Huang et al., 2016)

involved_in p38MAPK cascade

inferred from direct assay

(Han et al., 1998)

involved_in paracrine signaling

inferred from genetic interaction with FLYBASE:p38a; FB:FBgn0015765

(Lim et al., 2014)

involved_in positive regulation of actin filament polymerization

inferred from genetic interaction with FLYBASE:Mekk1; FB:FBgn0024329,FLYBASE:lic; FB:FBgn0261524

(Huang et al., 2016)

involved_in positive regulation of cell size

inferred from mutant phenotype

(Cully et al., 2010)

inferred from genetic interaction with FLYBASE:p38a; FB:FBgn0015765

(Cully et al., 2010)

involved_in positive regulation of multicellular organism growth

inferred from mutant phenotype

(Cully et al., 2010)

involved_in regulation of adult chitin-containing cuticle pigmentation

inferred from genetic interaction with FLYBASE:Ask1; FB:FBgn0014006

(Sekine et al., 2011)

involved_in regulation of BMP signaling pathway

inferred from direct assay

(Adachi-Yamada et al., 1999)

involved_in regulation of cellular response to oxidative stress

inferred from mutant phenotype

(Vrailas-Mortimer et al., 2011)

involved_in regulation of innate immune response

inferred from direct assay

(Han et al., 1998)

involved_in regulation of terminal button organization

inferred from mutant phenotype

(Klinedinst et al., 2013)

involved_in response to bacterium

inferred from direct assay

(Shinzawa et al., 2009)

involved_in response to heat

inferred from mutant phenotype

(Cully et al., 2010)

involved_in response to hydrogen peroxide

inferred from mutant phenotype

(Cully et al., 2010)

involved_in response to salt stress

inferred from mutant phenotype

(Seisenbacher et al., 2011, Cully et al., 2010)

inferred from genetic interaction with FLYBASE:Atf-2; FB:FBgn0265193

(Sano et al., 2005)

involved_in response to starvation

inferred from mutant phenotype

(Cully et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

involved_in MAPK cascade

non-traceable author statement

(Martin-Blanco, 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CONVENTIONAL MITOGEN ACTIVATED PROTEIN KINASES

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. (O61443)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity
MAP kinase activity

(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.11.24)

(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.11.24)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CONVENTIONAL MITOGEN ACTIVATED PROTEIN KINASES -: Conventional Mitogen-activated protein kinases (MAPK) are proline-directed protein serine/threonine kinases that are components of the MAPK signaling cascade. MAPKs are activated by dual phosphorylation on Thr and Tyr residues within a conserved Thr-X-Tyr motif located in the activation loop by MAPK kinases. (Adapted from PMID:21372320).

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Protein Function (UniProtKB)

Kinase involved in dpp signal transduction pathway in the process of wing morphogenesis when the levels of dpp are enhanced or inhibited. May down-regulate insect immunity gene expression after prolonged infection.

(UniProt, O61443)

Summary (Interactive Fly)

a MAP kinase involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis - regulates Hippo signaling in ovarian follicle cells and the wing disc

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\p38b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O61443)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

p38b-RA

FBtr0080534

NM_058013

1821

365

Additional Transcript Data and Comments

Reported size (kB)

1.143 (longest cDNA)

(Han et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

p38b-PA

FBpp0080111

42.1

365

6.45

O61443

NP_477361

AAF53326

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

365 (aa)

(Han et al., 1998)

Comments

External Data

Post Translational Modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

(UniProt, O61443)

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

(UniProt, O61443)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

O61443

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\p38b using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

p38b_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.71

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Han et al., 1998)

embryonic/larval anterior midgut

(Han et al., 1998)

embryonic/larval posterior midgut

(Han et al., 1998)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

larval stage

larval brain

(Vrailas-Mortimer et al., 2011)

Additional Descriptive Data

p38b transcripts are detected throughout embryonic development by in situ hybridization. There is a high level of maternal deposition. Later, expression is observed in most tissues with elevated levels in the anterior and posterior midguts.

(Han et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{p38b-lacZ.P}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

intestinal stem cell

(Park et al., 2010, Park et al., 2009)

adult midgut

(Park et al., 2009)

enteroblast

(Park et al., 2010)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\p38b in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\p38b

View exonic expression by tissue for Dmel\p38b

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024846

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34780/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0024846

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0024846

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0024846

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7393

Flygut - An atlas of the Drosophila adult midgut

FBgn0024846

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\p38b

Stages(s) 1-3
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 27 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of p38b

Transgenic constructs containing regulatory region of p38b

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2L)b80e3

(Paricio et al., 1999)

Df(2L)b87e25

(Weber et al., 2000, Paricio et al., 1999)

Df(2L)Δ11

(Vrailas-Mortimer et al., 2011)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die

p38b^d27

partially lethal - majority die | recessive

p38b^d27

viable

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

p38b^GL00140

Other Phenotypes

Allele

abnormal circadian rhythm, with Scer\GAL4^cry.PZ

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

abnormal circadian rhythm | progressive

p38b^Δ45

abnormal developmental rate

p38b^156A

abnormal heat stress response

p38b^Δ45

abnormal heat stress response, with Scer\GAL4^Mef2.PR

p38b^UAS.cAa

abnormal heat stress response | recessive

p38b^d27

abnormal immune response

p38b^156A

abnormal immune response | adult stage

p38b^ex9

abnormal immune response | adult stage, with Scer\GAL4^da.G32

abnormal immune response | adult stage, with Scer\GAL4^Pxn.PS

p38b^UAS.cAa

abnormal locomotor behavior

p38b^Δ45

abnormal locomotor behavior, with Scer\GAL4^Mef2.PR

p38b^{kd.UAS.Tag:FLAG}

abnormal neuroanatomy, with Scer\GAL4^cry.PZ

abnormal neuroanatomy, with Scer\GAL4^P0.5.Pdf

abnormal neuroanatomy | larval stage

p38b^Δ45

abnormal neuroanatomy | larval stage, with Scer\GAL4^BG380

p38b^DN.UAS

abnormal neuroanatomy | larval stage (with Df(2L)b80e3)

p38b^KG01337

abnormal neuroanatomy | larval stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

p38b^JF03341

abnormal osmotic stress response | recessive

p38b^d27

abnormal oxidative stress response

abnormal oxidative stress response, with Scer\GAL4^Mef2.PR

p38b^UAS.cAa

abnormal oxidative stress response, with Scer\GAL4^p38b-83

p38b^UAS.cAa

abnormal oxidative stress response, with Scer\GAL4^Ugt36A1.PK

p38b^DN.UAS

abnormal oxidative stress response, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

p38b^DN.UAS

abnormal starvation stress response, with Scer\GAL4^Mef2.PR

p38b^UAS.cAa

abnormal starvation stress response | recessive

p38b^d27

decreased body size | adult stage | recessive

p38b^d27

decreased cell size | adult stage | recessive

p38b^d27

increased mortality | conditional

p38b^156A

long lived, with Scer\GAL4^DJ757

p38b^UAS.cAa

long lived, with Scer\GAL4^EDTP-DJ694

p38b^UAS.cAa

long lived, with Scer\GAL4^how-24B

p38b^UAS.cAa

long lived, with Scer\GAL4^Mef2.PR

p38b^UAS.cAa

long lived, with Scer\GAL4^Mhc.PW

p38b^UAS.cAa

long lived, with Scer\GAL4^Mhc.PW, Scer\GAL80^ts.αTub84B

p38b^UAS.cAa

melanotic mass phenotype | drug conditional

p38b^156A

melanotic mass phenotype | larval stage

p38b^d27

melanotic mass phenotype | larval stage | nutrition conditional

p38b^d27

short lived | recessive

p38b^Δ45

visible, with Scer\GAL4^32B

p38b^DN.UAS

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^Ugt36A1.PK

p38b^DN.UAS

adult heart, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

p38b^DN.UAS

adult Malpighian tubule

p38b^156A

embryonic/larval hindgut | drug conditional

p38b^156A

embryonic/larval hindgut | larval stage | posterior

p38b^d27

embryonic/larval hindgut | larval stage | posterior | nutrition conditional

p38b^d27

NMJ bouton | larval stage

p38b^Δ45

NMJ bouton | larval stage, with Scer\GAL4^BG380

p38b^DN.UAS

NMJ bouton | larval stage (with Df(2L)b80e3)

p38b^KG01337

NMJ bouton | larval stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

p38b^JF03341

wing, with Scer\GAL4^32B

p38b^DN.UAS

wing vein, with Scer\GAL4^32B

p38b^DN.UAS

wing vein | ectopic, with Scer\GAL4^32B

p38b^DN.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (54)

Hsap\MAPK14

13 of 14

Yes

Hsap\MAPK11

11 of 14

Hsap\MAPK12

7 of 14

Yes

Hsap\MAPK13

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

Rnor\Mapk14

13 of 14

Yes

Rnor\Mapk11

11 of 14

Rnor\Mapk12

7 of 14

Yes

Rnor\Mapk13

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (24)

Mmus\Mapk14

13 of 14

Yes

Mmus\Mapk11

11 of 14

Mmus\Mapk12

6 of 14

Yes

Mmus\Mapk13

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (32)

Xtro\mapk11

7 of 13

Yes

Xtro\mapk14

7 of 13

Yes

Xtro\mapk13

5 of 13

Yes

Xtro\XB956263

5 of 13

Yes

4 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (34)

Drer\mapk14a

13 of 14

Yes

Drer\mapk14b

12 of 14

Yes

Drer\mapk11

11 of 14

Drer\mapk12b

7 of 14

Yes

Drer\mapk12a

6 of 14

Yes

Drer\mapk13

6 of 14

Yes

Drer\zgc:171775

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-45c16.2

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (30)

Cele\pmk-1

13 of 14

Yes

Cele\pmk-2

6 of 14

Yes

Cele\pmk-3

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP012148

8 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (51)

Atha\MPK3

5 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (12)

Scer\HOG1

11 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\sty1

11 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:p38b. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (23)

p38a

13 of 13

p38c

7 of 13

6 of 13

Erk7

5 of 13

bsk

4 of 13

nmo

4 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Doa

1 of 13

Dyrk2

1 of 13

mbt

1 of 13

Pak

1 of 13

Srpk79D

1 of 13

SRPK

1 of 13

wnd

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Salmonella species infection

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

p38b^DN.UAS

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cSa

(Sakakibara et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAPK14; mitogen-activated protein kinase 14

13 of 14

600289

MAPK11; mitogen-activated protein kinase 11

11 of 14

602898

MAPK12; mitogen-activated protein kinase 12

7 of 14

602399

MAPK13; mitogen-activated protein kinase 13

6 of 14

602899

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

p38b - Atf-2

enzymatic study, autoradiography

(Sano et al., 2005)

p38b - CG10418

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - CG14207

experimental knowledge based

(Guruharsha et al., 2011)

p38b - CG5721

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - CG7518

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - CG8360

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - CG9586

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Cdc16

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Ciz1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Clbn

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Belozerov et al., 2012)

p38b - Dlg5

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Glys

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Gyg

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Belozerov et al., 2012)

p38b - Jra

enzymatic study, autoradiography

(Sano et al., 2005, Han et al., 1998)

p38b - MAPk-Ak2

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, pull down, western blot, experimental knowledge based

(Belozerov et al., 2012, Guruharsha et al., 2011, Seisenbacher et al., 2011)

p38b - Npl4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - PTP-ER

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Rab1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Rack1

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Belozerov et al., 2014)

p38b - Rex2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - RfC3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - RfC4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - Sumo

pull down, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Handu et al., 2015)

p38b - dpa

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - eRF1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - holn1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - lic

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Belozerov et al., 2012)

p38b - mts

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - otu

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - p38a

experimental knowledge based

(Guruharsha et al., 2011)

p38b - per

enzymatic study, electrophoretic mobility-based method, autoradiography

(Dusik et al., 2014)

p38b - ps

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - puc

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - rad50

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - sd

anti tag coimmunoprecipitation, anti tag western blot

(Pimmett et al., 2017)

p38b - unc-13

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

p38b - ush

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

p38b

suppressible

hiw

(Klinedinst et al., 2013)

p38b

enhanceable

MAPk-Ak2

(Seisenbacher et al., 2011)

suppressible

(Li et al., 2020)

suppressible

(Vrailas-Mortimer et al., 2014)

p38b

suppressible

p38a

(Seisenbacher et al., 2011)

p38b

enhanceable

per

(Vrailas-Mortimer et al., 2014)

p38b

suppressible

per

(Vrailas-Mortimer et al., 2014)

p38a|p38b

suppressible

Sod2

(Vrailas-Mortimer et al., 2011)

p38b

suppressible

Sod2

(Vrailas-Mortimer et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ask1

suppressible

p38b

(Sekine et al., 2011)

Atf-2

enhanceable

p38b

(Sano et al., 2005)

enhanceable

p38b

(Adachi-Yamada et al., 1999)

Bub3

enhanceable

p38b

(Clemente-Ruiz et al., 2016)

suppressible

(Lim et al., 2014)

suppressible

(Lim et al., 2014)

enhanceable

(Adachi-Yamada et al., 1999)

hiw

suppressible

p38b

(Klinedinst et al., 2013)

suppressible

(Sun et al., 2019)

suppressible

(Vrailas-Mortimer et al., 2014)

Rala

enhanceable

p38b

(Balakireva et al., 2006)

Rala

suppressible

p38b

(Balakireva et al., 2006)

tkv

suppressible

p38b

(Adachi-Yamada et al., 1999)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60814

DroID - A comprehensive database of gene and protein interactions.

FBgn0024846

MIST (genetic) - An integrated Molecular Interaction Database

34780

MIST (protein-protein) - An integrated Molecular Interaction Database

34780

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O61443

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-49

Cytogenetic map

34D1-34D1

Sequence location

2L:13,780,689..13,782,611 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34D1-34D1

Limits computationally determined from genome sequence between P{lacW}CG7364^k07245 and P{lacW}Sos^k05224&P{lacW}RpII33^k05605

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

34D-34D

(Adachi-Yamada, 1997.8.6)

34C4-34D1

(Han, 1997.11.21)

34D-34D

(determined by in situ hybridisation)

(Adachi-Yamada et al., 1999, EDGP Project Members, 1994-)

34C4-34D1

(determined by in situ hybridisation)

(Han et al., 1998)

34C-34C

(determined by in situ hybridisation)

(Louis et al., 1997)

Experimentally Determined Recombination Data

Location

2-49

(FlyBase, 2016)

2-44.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (22)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

59005

P{UAS-p38b.DN}1, w^*/FM7c

29405

y¹ v¹; P{TRiP.JF03341}attP2

65523

w¹¹¹⁸; PBac{IT.GAL4}p38b^1237-G4

22319

y¹ w^*; P{EPgy2}p38b^EY11174

FlyORF

F001493

M{UAS-p38b.ORF}ZH-86Fb

F000971

M{UAS-p38b.ORF.3xHA}ZH-86Fb

F002309

M{UAS-p38b.ORF.3xHA.GW}ZH-86Fb

NIG-Fly

7393R-1

P{NIG.7393R}1

7393R-3

P{NIG.7393R}3

HMJ22183

y¹ v¹; P{TRiP.HMJ22183}attP40/CyO

VDRC

v330146

P{VSH330146}attP40

v108099

P{KK101754}VIE-260B

More stocks available...

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (164)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD24658

Other clones

DPiM_LD24658

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0024846

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0024846

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

p38b is expressed in clock neurons, and mutants in p38b either are arrhythmic or have a longer free-running periodicity, especially as they age.

(Vrailas-Mortimer et al., 2014)

p38b interacts with per to regulate period length and the strength of rhythmicity.

(Vrailas-Mortimer et al., 2014)

p38b activation in response to NaCl and heat shock is markedly reduced in Mekk1 mutants compared to wild type.

(Inoue et al., 2001)

Mekk1 regulation of the Mpk2/p38b pathway is critical for the response to environmental stresses.

(Inoue et al., 2001)

p38b functions in dpp signaling in wing morphogenesis.

(Adachi-Yamada et al., 1999)

Relationship to Other Genes

Source for database merge of

Additional comments

The p38a and p38b genes share at least 50% identity.

(Langille and Clark, 2007)

Nomenclature History

Source for database identify of

Source for identity of: p38b CG7393

(Guan, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

186F5S

(Louis et al., 1997)

BG:DS00797.3

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG7393

(National Institute of Genetics Fly Stocks, 2022-, Lin et al., 2018, Meadows, 2017.1.11, White-Grindley et al., 2014, Schertel et al., 2013, Ni et al., 2011.7.19, Vrailas-Mortimer et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Morrison et al., 2000)

D-p38

(Kurz and Ewbank, 2003)

D-p38 MAPK

(Ryabinina et al., 2006)

D-p38b

(Park et al., 2010, Park et al., 2009, Inoue et al., 2001, Martin-Blanco, 2000, Nebreda and Porras, 2000, Adachi-Yamada et al., 1999, Stronach and Perrimon, 1999, Han et al., 1998, Adachi-Yamada, 1997.8.6)

Dm p38b

(Shinzawa et al., 2007)

Dmp38b

(Shinzawa et al., 2009)

Dp38

(Sun et al., 2008, Kuranaga et al., 2002)

Dp38b

(Sekine et al., 2011)

ESTS:186F5S

MAPK

(Belozerov et al., 2014)

Mpk34C

P38

(El Chamy et al., 2015)

P38B

(Riahi et al., 2021)

anon-sts23

dp38b

(Tian et al., 2014, Shimizu et al., 2008)

p38 MAPK

(Shinzawa et al., 2007, Craig et al., 2000)

p38 MPK2b

(Macedo et al., 2020)

p38 β

(Muller et al., 2002)

p38^MAPK

(Zhang and Edgar, 2022)

p38B

(Manning, 2001.10.11)

p38Kb

(Ryan et al., 2021, Yu and Hyun, 2021, Vrailas-Mortimer et al., 2011)

p38MAPK

(Batista et al., 2016)

p38b

(Braun et al., 2025, Park et al., 2025, Wāng, 2025, Fangninou et al., 2024, Fangninou et al., 2023, Gera et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Fabian et al., 2021, Layalle et al., 2021, Nishida et al., 2021, Ostalé et al., 2021, Scepanovic et al., 2021, Schneider and Imler, 2021, Xie et al., 2021, Li et al., 2020, Tafesh-Edwards and Eleftherianos, 2020, Wang et al., 2020, Hegazi et al., 2019, Liaw and Chiang, 2019, Li et al., 2019, Lim et al., 2019, Sun et al., 2019, Sun et al., 2019, Romey-Glüsing et al., 2018, Eom et al., 2017, Houtz et al., 2017, Kenmoku et al., 2017, Lin et al., 2017, Liu and Jin, 2017, Nandi et al., 2017, Pimmett et al., 2017, Terriente-Félix et al., 2017, Transgenic RNAi Project members, 2017-, Ashton-Beaucage et al., 2016, Clandinin and Owens, 2016-, Huang et al., 2016, Schimizzi et al., 2016, Luo et al., 2015, Pasco et al., 2015, Santabárbara-Ruiz et al., 2015, Ugrankar et al., 2015, Zhan et al., 2015, Belozerov et al., 2014, Chakrabarti et al., 2014, Dusik et al., 2014, Lim et al., 2014, Sopko et al., 2014, White-Grindley et al., 2014, Wongtrakul et al., 2014, Carter, 2013, Freeman et al., 2013, Klinedinst et al., 2013, Schertel et al., 2013, Schoborg et al., 2013, Gomez et al., 2012, Gonda et al., 2012, Igboin et al., 2012, Vrailas-Mortimer et al., 2012, Wongtrakul et al., 2012, Mathew et al., 2011, Seisenbacher et al., 2011, Vrailas-Mortimer et al., 2011, Chen et al., 2010, Chen et al., 2010, Cully et al., 2010, Debat et al., 2009, Davis et al., 2008, Beltran et al., 2007, Langille and Clark, 2007, Liu et al., 2007, Sackton et al., 2007, Balakireva et al., 2006, Collins et al., 2006, Dworkin and Gibson, 2006, Dworkin and Gibson., 2006, Edwards et al., 2006, Sano et al., 2005, Cha et al., 2003)

p38β

(Ziech et al., 2023, Liu et al., 2019, Pereira et al., 2014, Bjorklund et al., 2006)

Name Synonyms

p38 MAP Kinase

(Vrailas-Mortimer et al., 2014)

p38 MAP kinase

(Sinenko et al., 2021)

p38 mitogen-activated protein kinase

(Belozerov et al., 2014, Balakireva et al., 2006)

p38b

(Edwards et al., 2006)

p38b MAP kinase

stress-activated p38b MAP kinase

(Han, 1997.11.21)

Secondary FlyBase IDs

FBgn0014421
FBgn0024951
FBgn0027921

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 76 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34780

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O61443

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O61443

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O61443

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024846

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34780/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0024846

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0024846

FlyMine - An integrated database for Drosophila genomics

FBgn0024846

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7393

MARRVEL_MODEL - MARRVEL (model organism gene)

34780

Linkouts

BioGRID - A database of protein and genetic interactions.

60814

Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0024846

DroID - A comprehensive database of gene and protein interactions.

FBgn0024846

DRSC - Results frm RNAi screens

FBgn0024846

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

p38b_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0024846

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0024846

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7393

Flygut - An atlas of the Drosophila adult midgut

FBgn0024846

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0024846

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013594

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

34780

MIST (protein-protein) - An integrated Molecular Interaction Database

34780

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O61443

References (210)

The following external sites may use different assemblies or annotations than FlyBase.

-0.71

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