FlyBase Allele Report: Dmel\p38b[DN.UAS]

General Information

Symbol

Dmel\p38b^DN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0096731

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

P{UAS-p38b.DN}

Also Known As

UAS-p38b^DN

Key Links

Transgenic product class

dominant_negative_variant

(Adachi-Yamada et al., 1999)

missense_variant

(Adachi-Yamada et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Adachi-Yamada et al., 1999)

missense_variant

(Adachi-Yamada et al., 1999)

Mutagen

in vitro construct

(Adachi-Yamada et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-p38b.DN}

Cytology

Description

A dominant negative form of p38b (carries the amino acid replacement T183A) is expressed under the control of UAS regulatory sequences.

(Adachi-Yamada et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

p38b

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cSa

(Sakakibara et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^BG380

(Klinedinst et al., 2013)

abnormal oxidative stress response, with Scer\GAL4^Ugt36A1.PK

(Lim et al., 2014)

abnormal oxidative stress response, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Lim et al., 2014)

visible, with Scer\GAL4^32B

(Adachi-Yamada et al., 1999)

Phenotype Manifest In

adult heart, with Scer\GAL4^Ugt36A1.PK

(Lim et al., 2014)

adult heart, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Lim et al., 2014)

NMJ bouton | larval stage, with Scer\GAL4^BG380

(Klinedinst et al., 2013)

wing, with Scer\GAL4^32B

(Adachi-Yamada et al., 1999)

wing vein, with Scer\GAL4^32B

(Adachi-Yamada et al., 1999)

wing vein | ectopic, with Scer\GAL4^32B

(Adachi-Yamada et al., 1999)

Detailed Description

Statement

Reference

Expression of p38b^DN.Scer\UAS in nonmyocytic pericardial cells, under the control of Scer\GAL4^Dot.PK results in the elevation of cardiac arrhthmias and a constriction of the cardiac tube in young (1 week) but not old (4 weeks) flies.

Expression of p38b^DN.Scer\UAS in the cardiomyocytes, under the control of Scer\GAL4^Scer\UAS.cHa/Scer\GAL4^tin.cBa has no effect on cardiac function and morphology.

Overexpression of p38b^DN.Scer\UAS in nonmyocytic pericardial cells during embryogenesis and larval stages, under the control of Scer\GAL4^Dot.PK and Scer\GAL80^ts.αTub84B results in significant cardiac arrhythmias, compared to controls. Moreover, this perturbation of pericardial reactive oxygen species-p38b signaling leads to a significant thinning of the cardiac tube.

Overexpression of p38b^DN.Scer\UAS in nonmyocytic pericardial cells during the pupal and adult stages only, under the control of Scer\GAL4^Dot.PK and Scer\GAL80^ts.αTub84B, is associated with a significant narrowing of the cardiac tube but does not affect heart rhythm.

(Lim et al., 2014)

Expression of p38b^DN.Scer\UAS under the control of Scer\GAL4^BG380 results in an increase in maximum bouton diameter at the larval neuromuscular junction compared to controls.

(Klinedinst et al., 2013)

Some escapers expressing two copies of p38b^DN.Scer\UAS under the control of two copies of Scer\GAL4^32B have ectopic vein fragments around the end of vein L2. They show a reduction in the distance between veins L4 and L5.

(Adachi-Yamada et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

p38b^DN.UAS, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\MAPT^UAS.cSa, Scer\GAL4^GMR.PF

(Sakakibara et al., 2023)

p38b^DN.UAS, Scer\GAL4^sca-537.4 is an enhancer of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

p38b^DN.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Atf-2^bZIP.UAS, Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

p38b^DN.UAS, Scer\GAL4^hs.PB is an enhancer of abnormal osmotic stress response phenotype of Atf-2^bZIP.UAS, Scer\GAL4^hs.PB

(Sano et al., 2005)

Scer\GAL4^32B/p38b^DN.UAS is an enhancer of visible | recessive phenotype of dpp^d5

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B/p38b^DN.UAS is an enhancer of visible | recessive phenotype of dpp^hr27

(Adachi-Yamada et al., 1999)

p38b^DN.UAS/Scer\GAL4^69B is an enhancer of visible | recessive phenotype of bi^omb-1

(Adachi-Yamada et al., 1999)

Suppressor of

Statement

Reference

Scer\GAL4^71B/p38b^DN.UAS is a suppressor of visible phenotype of Scer\GAL4^71B, tkv^CA.UAS

(Adachi-Yamada et al., 1999)

NOT Suppressor of

Statement

Reference

p38b^DN.UAS/Scer\GAL4^ptc.PU is a non-suppressor of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

p38b^DN.UAS, Scer\GAL4^ptc.PU is a non-suppressor of abnormal cell migration | third instar larval stage phenotype of Hsap\MAP2K3^UAS.cSa, Scer\GAL4^ptc.PU

(Sun et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

p38b^DN.UAS, Scer\GAL4^GMR.PF is an enhancer of lamina phenotype of Hsap\MAPT^UAS.cSa, Scer\GAL4^GMR.PF

(Sakakibara et al., 2023)

p38b^DN.UAS, Scer\GAL4^GMR.PF is an enhancer of axon phenotype of Hsap\MAPT^UAS.cSa, Scer\GAL4^GMR.PF

(Sakakibara et al., 2023)

p38b^DN.UAS, Scer\GAL4^sca-537.4 is an enhancer of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

p38b^DN.UAS, Scer\GAL4^sca-537.4 is an enhancer of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

p38b^DN.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of Atf-2^bZIP.UAS, Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Scer\GAL4^32B/p38b^DN.UAS is an enhancer of wing phenotype of dpp^d5

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B/p38b^DN.UAS is an enhancer of wing phenotype of dpp^hr27

(Adachi-Yamada et al., 1999)

p38b^DN.UAS/Scer\GAL4^69B is an enhancer of wing phenotype of bi^omb-1

(Adachi-Yamada et al., 1999)

p38b^DN.UAS/Scer\GAL4^69B is an enhancer of wing vein phenotype of bi^omb-1

(Adachi-Yamada et al., 1999)

Suppressor of

Statement

Reference

p38b^DN.UAS/Scer\GAL4^ptc.PU is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

Scer\GAL4^71B/p38b^DN.UAS is a suppressor of wing vein phenotype of Scer\GAL4^71B, tkv^CA.UAS

(Adachi-Yamada et al., 1999)

p38b^DN.UAS/Scer\GAL4^69B is a suppressor of wing disc phenotype of Scer\GAL4^69B, tkv^CA.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B/p38b^DN.UAS is a suppressor of wing phenotype of Scer\GAL4^71B, tkv^CA.UAS

(Adachi-Yamada et al., 1999)

NOT Suppressor of

Statement

Reference

p38b^DN.UAS, Scer\GAL4^ptc.PU is a non-suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Hsap\MAP2K3^UAS.cSa, Scer\GAL4^ptc.PU

(Sun et al., 2019)

p38a¹, Scer\GAL4^BG380, p38b^DN.UAS is a non-suppressor of neuromuscular junction phenotype of hiw^ND8

(Collins et al., 2006)

p38a¹, Scer\GAL4^BG380, p38b^DN.UAS is a non-suppressor of bouton phenotype of hiw^ND8

(Collins et al., 2006)

p38b^DN.UAS/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, rpr^UAS.cIa

(Kuranaga et al., 2002)

p38b^DN.UAS/Scer\GAL4^unspecified is a non-suppressor of wing phenotype of Egfr^E1

(Adachi-Yamada et al., 1999)

p38b^DN.UAS/Scer\GAL4^unspecified is a non-suppressor of wing phenotype of N^Ax-1

(Adachi-Yamada et al., 1999)

Other

Statement

Reference

Scer\GAL4^32B, dpp^d5, p38b^DN.UAS has wing vein L5 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5, p38b^DN.UAS has wing vein phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5, p38b^DN.UAS has wing vein L4 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^hr27, p38b^DN.UAS has wing vein L5 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^hr27, p38b^DN.UAS has wing vein phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^hr27, p38b^DN.UAS has wing vein L4 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5/dpp^hr27, p38b^DN.UAS has wing vein L5 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5/dpp^hr27, p38b^DN.UAS has wing vein phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5/dpp^hr27, p38b^DN.UAS has wing vein L4 phenotype

(Adachi-Yamada et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of p38b^DN.Scer\UAS (under the control of Scer\GAL4^BG380) and Mpk2¹ does not suppress the hiw^ND8 synaptic phenotype.

(Collins et al., 2006)

Coexpression of Atf-2^{bZIP.Scer\UAS} and p38b^DN.Scer\UAS, under the control of Scer\GAL4^Bx-MS1096, there is an enhancement of the wing formation defects seen when only Atf-2^{bZIP.Scer\UAS} is expressed. In contrast, expressing only p38b^DN.Scer\UAS does not lead to visible wing defects.

Coexpression of Atf-2^{bZIP.Scer\UAS} and p38b^DN.Scer\UAS, under the control of Scer\GAL4^hs.PB, enhances the salt-sensitive phenotype of embryos seen when Atf-2^{bZIP.Scer\UAS} is expressed alone.

(Sano et al., 2005)

The wing phenotype of dpp^d5/dpp^hr27 flies is strongly enhanced by two copies of p38b^DN.Scer\UAS expressed under the control of one copy of Scer\GAL4^32B; veins L4 and L5 are partially fused and the distance between veins L2 and L3 is reduced. Suppresses the phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B. This suppression is reduced if the flies are also coexpressing p38b^Scer\UAS.cAa. Expression of p38b^DN.Scer\UAS under the control of Scer\GAL4^unspecified has no significant effect on the wing phenotype caused by Egfr^E1 or N^Ax-1. Enhances the wing phenotype of bi^omb-1/Y flies.

(Adachi-Yamada et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59005

P{UAS-p38b.DN}1, w^*/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

D-p38b^DN

(Cha et al., 2003)

p38b^DN.Scer\UAS

p38b^DN.UAS

p38b^DN

(Balakireva et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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