FlyBase Allele Report: Dmel\tkv[CA.UAS]

General Information

Symbol

Dmel\tkv^CA.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0096660

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv.CA}

Also Known As

UAS-tkv^CA, UAS-tkv*, tkv-CA, UAS-TkvCA

Key Links

Transgenic product class

missense_variant

(Adachi-Yamada et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Adachi-Yamada et al., 1999)

Mutagen

in vitro construct

(Adachi-Yamada et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-tkv.CA}

Cytology

Description

A constitutively active form of the short isoform of tkv, in which a Gln residue in the GS domain has been replaced by an acidic residue, is expressed under the control of UAS regulatory sequences.

(Adachi-Yamada et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tkv

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Nahm et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Nahm et al., 2013)

partially lethal - majority die, with Scer\GAL4^Mef2.PU

(Galeone et al., 2017)

visible, with Scer\GAL4^71B

(Adachi-Yamada et al., 1999, Adachi-Yamada et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of tkv^CA.Scer\UAS under the control of Scer\GAL4^Mef2.PU results in increased mortality before adulthood, as compared to controls.

(Galeone et al., 2017)

Expression of tkv^CA.Scer\UAS under the control of Scer\GAL4^elav-C155 results in a significant increase in bouton number and satellite bouton number at the neuromuscular junction in third instar larvae.

Expression of tkv^CA.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 results in vacuolisation and increased cell death in the brains of 20 day old flies.

(Nahm et al., 2013)

Scer\GAL4¹¹⁵¹ driven tkv^CA.Scer\UAS engenders an approximately two-fold increase in the number of tergal depressor of trochanter (TDT) muscle fibers, although each individual fiber is somewhat smaller than in wild-type muscle.

An increase in the number of TDT founder cells is clearly visible by 16 hours after puparium formation (APF) as a result of Scer\GAL4¹¹⁵¹ driven tkv^CA.Scer\UAS expression.

(Jaramillo et al., 2009)

Normal wing venation is severely distorted when tkv^CA.Scer\UAS is expressed under the control of Scer\GAL4^71B, and extensive production of fragments of vein material is seen. The abdominal cuticle pattern is irregular. The wing phenotype is significantly suppressed by exposure of the larvae to the imidazole compound SB203580 (a p38 inhibitor). The wing phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B is not suppressed by tkv⁷.

(Adachi-Yamada et al., 1999)

Flies expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B often show notching of the wing margin. The notches are located away from the distal tip of the wing and are limited to the more proximal region. Dying cells are distributed throughout the primordium of the proximal wing in wing discs expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B but are absent from the distal wing primordium. When tkv^CA.Scer\UAS is expressed mosaically in the wing primordia using Scer\GAL4^Act5C.PI, dying cells are seen in clones in the proximal wing but not in the distal wing.

(Adachi-Yamada et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^71B, tkv^CA.UAS has visible phenotype, suppressible by Scer\GAL4^71B/p38b^a.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has visible phenotype, suppressible by Scer\GAL4^71B/p38b^DN.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has visible phenotype, suppressible by Df(2L)C28

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has visible phenotype, suppressible by Df(2L)b82a2

(Adachi-Yamada et al., 1999)

Suppressor of

Statement

Reference

tkv^CA.UAS/Scer\GAL4^VGlut1-OK371 is a suppressor of abnormal flight phenotype of myd^3PM71

(Sidisky et al., 2021)

tkv^CA.UAS/Scer\GAL4^Mef2.PU is a suppressor of partially lethal - majority die phenotype of Pngl^ex14

(Galeone et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^71B, tkv^CA.UAS has abdomen phenotype, suppressible by Scer\GAL4^71B/p38b^a.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, suppressible by Scer\GAL4^71B/p38b^a.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing vein phenotype, suppressible by Scer\GAL4^71B/p38b^a.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, suppressible by Scer\GAL4^71B/p38b^DN.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing vein phenotype, suppressible by Scer\GAL4^71B/p38b^DN.UAS

(Adachi-Yamada et al., 1999)

Scer\GAL4^69B, tkv^CA.UAS has wing disc phenotype, suppressible by p38b^a.UAS/Scer\GAL4^69B

(Adachi-Yamada et al., 1999)

Scer\GAL4^69B, tkv^CA.UAS has wing disc phenotype, suppressible by p38b^DN.UAS/Scer\GAL4^69B

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, suppressible by Df(2L)C28

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing vein phenotype, suppressible by Df(2L)C28

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, suppressible by Df(2L)b82a2

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing vein phenotype, suppressible by Df(2L)b82a2

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing disc phenotype, suppressible by hep^r75

(Adachi-Yamada et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, non-suppressible by Df(3R)crb-F89-4

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, non-suppressible by Df(2L)flp147E

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, non-suppressible by dpp^d8

(Adachi-Yamada et al., 1999)

Scer\GAL4^71B, tkv^CA.UAS has wing phenotype, non-suppressible by bsk^DN.UAS/Scer\GAL4^71B

(Adachi-Yamada et al., 1999)

Suppressor of

Statement

Reference

tkv^CA.UAS/Scer\GAL4^Mef2.PU is a suppressor of embryonic/larval midgut | larval stage phenotype of Pngl^ex14

(Galeone et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of tkv^CA.Scer\UAS under the control of Scer\GAL4^Mef2.PU suppresses the near lethality of Pngl^ex14 homozygotes and the decreased acid zone of their larval midgut.

(Galeone et al., 2017)

Suppression by Df(2L)b82a2 or p38b^DN.Scer\UAS (expressed under the control of Scer\GAL4^71B) of the wing phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B is reduced if the flies are also coexpressing p38b^Scer\UAS.cAa. The wing disc is overgrown if tkv^CA.Scer\UAS is expressed under the control of Scer\GAL4^69B. This phenotype is strongly suppressed by coexpression of either p38b^a.Scer\UAS or p38b^DN.Scer\UAS.

(Adachi-Yamada et al., 1999)

The cell death seen in the wing discs of larvae expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B is suppressed if the larvae are also hemizygous for hep^r75.

(Adachi-Yamada et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36537

w^*; P{UAS-tkv.CA}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tkv^CA.Scer\UAS

tkv^CA.UAS

Name Synonyms

Secondary FlyBase IDs

References (15)

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