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General Information
Symbol
Dmel\tkvCA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0096660
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-tkvCA
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A constitutively active form of the short isoform of tkv, in which a Gln residue in the GS domain has been replaced by an acidic residue, is expressed under the control of UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of tkvCA.Scer\UAS under the control of Scer\GAL4Mef2.PU results in increased mortality before adulthood, as compared to controls.

Expression of tkvCA.Scer\UAS under the control of Scer\GAL4elav-C155 results in a significant increase in bouton number and satellite bouton number at the neuromuscular junction in third instar larvae.

Expression of tkvCA.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in vacuolisation and increased cell death in the brains of 20 day old flies.

Scer\GAL41151 driven tkvCA.Scer\UAS engenders an approximately two-fold increase in the number of tergal depressor of trochanter (TDT) muscle fibers, although each individual fiber is somewhat smaller than in wild-type muscle.

An increase in the number of TDT founder cells is clearly visible by 16 hours after puparium formation (APF) as a result of Scer\GAL41151 driven tkvCA.Scer\UAS expression.

When tkvCA.Scer\UAS clones are made in the wing disc (driven by Scer\GAL4Act5C.PI) produces clones with a rounded appearance.

Normal wing venation is severely distorted when tkvCA.Scer\UAS is expressed under the control of Scer\GAL471B, and extensive production of fragments of vein material is seen. The abdominal cuticle pattern is irregular. The wing phenotype is significantly suppressed by exposure of the larvae to the imidazole compound SB203580 (a p38 inhibitor). The wing phenotype caused by tkvCA.Scer\UAS expressed under the control of Scer\GAL471B is not suppressed by tkv7.

Flies expressing tkvCA.Scer\UAS under the control of Scer\GAL471B often show notching of the wing margin. The notches are located away from the distal tip of the wing and are limited to the more proximal region. Dying cells are distributed throughout the primordium of the proximal wing in wing discs expressing tkvCA.Scer\UAS under the control of Scer\GAL471B but are absent from the distal wing primordium. When tkvCA.Scer\UAS is expressed mosaically in the wing primordia using Scer\GAL4Act5C.PI, dying cells are seen in clones in the proximal wing but not in the distal wing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The expression of tkvCA.Scer\UAS under the control of Scer\GAL4Mef2.PU suppresses the near lethality of Pnglex14 homozygotes and the decreased acid zone of their larval midgut.

Coexpression of eyScer\UAS.cHa and tkvCA.Scer\UAS under control of Scer\GAL4hs.2sev results in large ectopic "eyes" (using antibodies against elav to visualize eye differentiation) in both wing and leg discs in regions far from the anterior-posterior compartment boundary.

Suppression by Df(2L)b82a2 or p38bDN.Scer\UAS (expressed under the control of Scer\GAL471B) of the wing phenotype caused by tkvCA.Scer\UAS expressed under the control of Scer\GAL471B is reduced if the flies are also coexpressing p38bScer\UAS.cAa. The wing disc is overgrown if tkvCA.Scer\UAS is expressed under the control of Scer\GAL469B. This phenotype is strongly suppressed by coexpression of either p38ba.Scer\UAS or p38bDN.Scer\UAS.

The cell death seen in the wing discs of larvae expressing tkvCA.Scer\UAS under the control of Scer\GAL471B is suppressed if the larvae are also hemizygous for hepr75.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
tkvCA.Scer\UAS
tkvCA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (12)