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General Information
Symbol
Dmel\dppd8
Species
D. melanogaster
Name
FlyBase ID
FBal0003005
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutagen
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Caused by aberration
Cytology
Nature of the lesion
Statement
Reference

Breakpoint allele.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The wings of dppd8/dppd12 mutants do not develop at all, only rudimentary stubs are formed, this can be rescued by expression of Ecol\lexALG.dpp>dpplexAop.cHa.EGFP : the wings of these adults display venation defects (extra vein material at the anterior crossvein, loss of distal part of L4) and the have slightly elongated shape but their sizes are comparable to that of control wings. The wing size of these rescued flies can in turn be reduced again by hindering the spreading of the dpp morphogen: completely blocking it by co-expressing Zzzz\vhhGFP4Ecol\lexAop.morphotrap.ext.T:Disc\RFP-mCherry at the source, i.e. within the dpp expression domain or reducing it by expressing Zzzz\vhhGFP4Scer\UAS.morphotrap.ext.T:Disc\RFP-mCherry under the control of Scer\GAL4hh.PU (i.e. in the posterior compartment). The former blocks the wing blade development completely, with the latter the blade size is strongly decreased and the patterning in its posterior part is lost.

Blocking dpp spreading at its source however does not interfere with the uniform proliferation pattern in larval imaginal discs as the density of mitotic cells (pH3-positive) is comparable between the rescue and morphotrap discs. Clonal growth rates are also not generally affected (assessed by utilizing the Raeppli tissue-labelling tool), but in the morphotrap wing discs low numbers of very small clones (1-3) are found next to the A/P boundary and these are not observed in the control discs.

In wing discs where dpp spreading is blocked within the dpp expression domain, the growth of the lateral part of the wing compartments shows similar width increase and similar growth rate as in the control discs with dpp spreading unhindered. However, the proliferation of the medial cell population (marked by low brk) is abolished, so the overall size of wing disc is visibly decreased compared to controls.

Similarly, hindering the dpp spreading at its (anterior) source reduces the size of the posterior wing pouch by more than 60%, blocking the dpp spreading in the posterior compartment (by expressing the morphotrap under the control of Scer\GAL4hh.PU) decreased the posterior pouch size by about 40%.

dppd8/dppd10 mutants exhibit small eyes and reduced or absent wings.

The wing blade is almost entirely missing in dppd8/dppd12 animals. Some wing hinge structures are present.

dppd8/dpphr56 flies show a small notching of the wing tip in less than 10% of cases.

dppd8/dppd12 flies have rudimentary wings.

The external genitalia of dppd8/dppd12 males are reduced to a small ring like structure. dppd8/dppd12 females show a complete duplication of the thorn bristles of the vaginal plate.

dppd6/dppd8 transheterozygotes have defects in both wing and leg development. Mutants can produce ventral wing hinge structures in transdetermined legs.

dppd8/dppd12 dics wing pouch is small, almost no wing tissue remains, except structures for the proximal hinge region. The proximal costa remains in the anterior compartment and a reduced alula in the posterior. Partial rescue of the wing phenotype is achieved by tkvQ253D.Scer\UAS.cLa expression driven by Scer\GAL4dpp.blk1.

Adults lack distal portion of appendages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

dpphr56/dppd8 has visible phenotype, enhanceable by pucE69/puc[+]

dpphr56/dppd8 has visible phenotype, enhanceable by hep[+]/hepCA

Enhancer of
Statement
Reference

dppd8 is an enhancer of visible phenotype of bi1

Phenotype Manifest In
Enhanced by
Statement
Reference

dpphr56/dppd8 has wing phenotype, enhanceable by pucE69/puc[+]

dpphr56/dppd8 has wing phenotype, enhanceable by hep[+]/hepCA

Enhancer of
Statement
Reference

dppd8 is an enhancer of wing phenotype of bi1

NOT Suppressor of
Statement
Reference

dppd8 is a non-suppressor of wing phenotype of Scer\GAL471B, tkvCA.UAS

Additional Comments
Genetic Interactions
Statement
Reference

The dppd8/dpphr56 phenotype is significantly enhanced by a single copy of pucE69 or hepCA, and in the case of hepCA a wide region of the wing blade is lost, leaving only the base of the wing.

brkM68 clones induced in a dppd8/dppd12 background lead to outgrowths of wing blade material indicating that normal target gene activation occurs without normal dpp levels.

Very few wgAct5C.PS; dppd6/dppd8 flies exhibit wg-induced transdetermination, legs with wing tissue.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

dppScer\UAS.T.T:Avic\GFP-EGFP rescues the wing phenotype of dppd8/dppd12 flies when expressed under the control of Scer\GAL4dpp.blk1; wing size and pattern are almost normal except that ectopic vein material is seen near vein 3 and the spacing between veins 3 and 4 is slightly increased.

Belongs to the disk-III class of alleles.

Allele class: d-III

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

dpp class III allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (17)