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Citation
Harmansa, S., Hamaratoglu, F., Affolter, M., Caussinus, E. (2015). Dpp spreading is required for medial but not for lateral wing disc growth.  Nature 527(7578): 317--322.
FlyBase ID
FBrf0230208
Publication Type
Research paper
Abstract

Drosophila Decapentaplegic (Dpp) has served as a paradigm to study morphogen-dependent growth control. However, the role of a Dpp gradient in tissue growth remains highly controversial. Two fundamentally different models have been proposed: the 'temporal rule' model suggests that all cells of the wing imaginal disc divide upon a 50% increase in Dpp signalling, whereas the 'growth equalization model' suggests that Dpp is only essential for proliferation control of the central cells. Here, to discriminate between these two models, we generated and used morphotrap, a membrane-tethered anti-green fluorescent protein (GFP) nanobody, which enables immobilization of enhanced (e) GFP::Dpp on the cell surface, thereby abolishing Dpp gradient formation. We find that in the absence of Dpp spreading, wing disc patterning is lost; however, lateral cells still divide at normal rates. These data are consistent with the growth equalization model, but do not fit a global temporal rule model in the wing imaginal disc.

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PubMed Central ID
Related Publication(s)
Note

Developmental Biology: Decapentaplegic Controls Growth at a Distance.
Vincent et al., 2016, Curr. Biol. 26(5): R209--R212 [FBrf0231226]

Morphogens: How to grow wings.
Strzyz, 2016, Nat. Rev. Mol. Cell Biol. 17(1): 1 [FBrf0232039]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nature
    Title
    Nature
    Publication Year
    1869-
    ISBN/ISSN
    0028-0836
    Data From Reference