FlyBase Allele Report: Dmel\Atf-2[bZIP.UAS]

General Information

Symbol

Dmel\Atf-2^bZIP.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0215652

Feature type

allele

Associated gene

Dmel\Atf-2

Associated Insertion(s)

Carried in Construct

P{UAS-Atf-2.bZIP}

Key Links

Transgenic product class

inframe_deletion

(Sano et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Sano et al., 2005)

Mutagen

in vitro construct

(Sano et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Atf-2.bZIP}

Cytology

Description

UASt regulatory sequences drive expression of the C-terminal domain of Atf-2 (amino acid residues 230-381).

(Sano et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atf-2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal osmotic stress response, with Scer\GAL4^hs.PB

(Sano et al., 2005)

visible, with Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Phenotype Manifest In

wing vein, with Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Detailed Description

Statement

Reference

Expression of Atf-2^{bZIP.Scer\UAS} in the wing disc, under the control of Scer\GAL4^Bx-MS1096, results in the distortion of wing venation and extensive production of fragments of vein material.

Embryos that express Atf-2^{bZIP.Scer\UAS} under the control of Scer\GAL4^hs.PB show normal viability when bred in normal culture medium. However, if the culture medium contains 0.2M NaCl, their viability decreases to ~40% of controls.

(Sano et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atf-2^bZIP.UAS, Scer\GAL4^Bx-MS1096 has visible phenotype, enhanceable by p38b^DN.UAS, Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Atf-2^bZIP.UAS, Scer\GAL4^hs.PB has abnormal osmotic stress response phenotype, enhanceable by p38b^DN.UAS, Scer\GAL4^hs.PB

(Sano et al., 2005)

Suppressed by

Statement

Reference

Atf-2^bZIP.UAS, Scer\GAL4^hs.PB has abnormal osmotic stress response phenotype, suppressible | partially by Mekk1^UAS.cIa, Scer\GAL4^hs.PB

(Sano et al., 2005)

Suppressor of

Statement

Reference

Atf-2^bZIP.UAS, Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of Mekk1^UAS.cIa, Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Atf-2^bZIP.UAS, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by p38b^DN.UAS, Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Suppressor of

Statement

Reference

Atf-2^bZIP.UAS, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Mekk1^UAS.cIa, Scer\GAL4^Bx-MS1096

(Sano et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Atf-2^{bZIP.Scer\UAS} and p38b^DN.Scer\UAS, under the control of Scer\GAL4^Bx-MS1096, there is an enhancement of the wing formation defects seen when only Atf-2^{bZIP.Scer\UAS} is expressed. In contrast, expressing only p38b^DN.Scer\UAS does not lead to visible wing defects.

Coexpression of Atf-2^{bZIP.Scer\UAS} with Mekk1^Scer\UAS.cIa, under the control of Scer\GAL4^Bx-MS1096, results in a partial suppression of the Mekk1^Scer\UAS.cIa-induced wing phenotype.

Coexpression of Atf-2^{bZIP.Scer\UAS} and p38b^DN.Scer\UAS, under the control of Scer\GAL4^hs.PB, enhances the salt-sensitive phenotype of embryos seen when Atf-2^{bZIP.Scer\UAS} is expressed alone. In contrast, coexpression of Mekk1^Scer\UAS.cIa partially suppresses the Scer\GAL4^hs.PB>Atf-2^{bZIP.Scer\UAS} salt sensitivity.

(Sano et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atf-2^{bZIP.Scer\UAS}

Atf-2^bZIP.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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