FlyBase Allele Report: Dmel\p38b[Δ25]

General Information

Symbol

Dmel\p38b^Δ25

Species

D. melanogaster

Name

FlyBase ID

FBal0264803

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

Also Known As

p38Kb^Δ25

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Vrailas-Mortimer et al., 2011)

Progenitor genotype

p38b^KG01337

(Vrailas-Mortimer et al., 2011)

Cytology

Description

Excision of P{SUPor-P}p38b^KG01337 results in the removal of 299bp upstream of the transcription start.

(Vrailas-Mortimer et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:13,780,612..13,780,910 [+]

Comment:

Estimated boundaries of a 299bp deletion resulting from the excision of P{SUPor-P}p38b^KG01337 that removes sequences upstream of the p38b coding region.

(Vrailas-Mortimer et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Vrailas-Mortimer et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

p38a¹, p38b^Δ25 has abnormal locomotor behavior | adult stage phenotype, suppressible by p38b^UAS.cAa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has short lived phenotype, suppressible by p38b^UAS.cAa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal oxidative stress response phenotype, suppressible by p38b^UAS.cAa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has partially lethal - majority die phenotype, suppressible by p38b^UAS.cAa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has partially lethal - majority die phenotype, suppressible by Sod2^UAS.cMa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

NOT suppressed by

Statement

Reference

p38a¹, p38b^Δ25 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Scer\GAL4^elav-C155/p38b^UAS.cAa

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/p38b^UAS.cAa

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has partially lethal - majority die phenotype, non-suppressible by p38b^ala.UAS/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has partially lethal - majority die phenotype, non-suppressible by Sod1^UAS.cAa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has partially lethal - majority die phenotype, non-suppressible by Cat^UAS.cAa/Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

Other

Statement

Reference

p38a¹, p38b^Δ25 has abnormal circadian rhythm phenotype

(Vrailas-Mortimer et al., 2014)

p38a¹, p38b^Δ25 has abnormal neuroanatomy | larval stage phenotype

(Klinedinst et al., 2013)

p38a¹, p38b^Δ25/p38b[+] has abnormal stress response phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal starvation stress response phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal oxidative stress response phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has lethal | pupal stage | conditional phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal locomotor behavior | adult stage phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25/p38b[+] has abnormal size | pupal stage | conditional phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has partially lethal - majority die phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has short lived phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal stress response phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal heat stress response phenotype

(Vrailas-Mortimer et al., 2011)

p38a¹, p38b^Δ25 has abnormal flight phenotype

(Vrailas-Mortimer et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

p38a¹, p38b^Δ25 has NMJ bouton | larval stage phenotype

(Klinedinst et al., 2013)

p38a¹, p38b^Δ25/p38b[+] has pupa | conditional phenotype

(Vrailas-Mortimer et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

p38b^Δ25/p38a¹ heterozygous mutants do not display defects in locomotor behaviour.

A significant proportion of p38b^Δ25/p38a¹ heterozygous mutants display arrhythmic circadian behaviour.

Approximately 38% of p38b^Δ25/p38a¹ heterozygous mutants display ultradian rhythms compared with 4% of wild-type controls.

(Vrailas-Mortimer et al., 2014)

p38b^Δ25 ; Mpk2¹ larvae show an increase in maximum bouton diameter at the neuromuscular junction compared to controls.

(Klinedinst et al., 2013)

p38b^Δ25 Mpk2¹ double mutant adults appear normal on eclosion but have a severely reduced lifespan compared to controls.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR significantly rescues the reduction in lifespan seen in p38b^Δ25 Mpk2¹ double mutants. No rescue is seen when p38b^Scer\UAS.cAa is expressed in neurons under the control of Scer\GAL4^elav-C155.

p38b^Δ25 Mpk2¹ double mutant females exhibit age-dependent impairment in flight behaviour. Negative geotaxis is also prominently impaired and deteriorates more rapidly with age. Aberrant walking behaviour is seen, with 3 day old p38b^Δ25 Mpk2¹ double mutants exhibiting various distortions that include a tendency to drag the abdomen, dragging a leg, shuffling of the metathoracic legs and frequent slippage. Walking speed is reduced compared to controls and the improvement in walking ability with age seen in wild type flies does not occur.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR suppresses the geotaxis and walking defects seen in p38b^Δ25 Mpk2¹ double mutant females. No suppression is seen when p38b^Scer\UAS.cAa is expressed pan-neuronally under the control of Scer\GAL4^elav-C155.

No age-dependent deterioration in the function of the giant-fiber system is observed in p38b^Δ25 Mpk2¹ double mutants.

Almost all p38b^Δ25 Mpk2¹ 1-2 day old double mutant flies die within 24 hours of heat shock at 37[o]C for 5 hours, in contrast to an almost 100% survival rate in controls. When reared under conditions of dry starvation, 50% of p38b^Δ25 Mpk2¹ double mutant animals die within 15 hours, compared to 20% of controls.

p38b^Δ25 Mpk2¹ double mutant flies are significantly more sensitive to hydrogen peroxide-induced oxidative stress compared to controls. Lifespan is also reduced when adult flies are exposed to the oxidising herbicide paraquat.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR significantly rescues the increased sensitivity to hydrogen peroxide-induced oxidative stress seen in p38b^Δ25 Mpk2¹ double mutants.

Only 17% of p38b^Δ25 Mpk2¹ double mutant flies make it to adulthood compared to controls.

Expression of p38b^Scer\UAS.cAa in muscles under the control of Scer\GAL4^Mef2.PR completely rescues the reduced viability seen in p38b^Δ25 Mpk2¹ double mutants.

Expression of p38b^ala.Scer\UAS in muscles under the control of Scer\GAL4^Mef2.PR does not suppress the reduction in viability seen in p38b^Δ25 Mpk2¹ double mutants.

Expression of Sod2^Scer\UAS.cMa under the control of Scer\GAL4^Mef2.PR partially rescues the reduced viability seen in p38b^Δ25 Mpk2¹ double mutants.

Expression of Sod^Scer\UAS.cAa under the control of Scer\GAL4^Mef2.PR does not rescue the reduced viability seen in p38b^Δ25 Mpk2¹ double mutants.

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^Mef2.PR does not rescue the reduced viability seen in p38b^Δ25 Mpk2¹ double mutants.

p38b^Δ25 Mpk2¹ double mutants fail to eclose when grown under chronic hypoxic conditions. Lifespan is equally short in hypoxic and normoxic conditions.

Homozygous Mpk2¹ mutants with one copy of p38b^Δ25 have smaller pupae and adults under hypoxic conditions compared to normoxia.

(Vrailas-Mortimer et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p38Kb^Δ25

(Vrailas-Mortimer et al., 2014, Vrailas-Mortimer et al., 2011)

p38b^Δ25

(Dusik et al., 2014, Klinedinst et al., 2013, Schoborg et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (5)

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