FlyBase Allele Report: Dmel\p38b[ala.UAS]

General Information

Symbol

Dmel\p38b^ala.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0264807

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

P{UAS-p38b.ala}

Key Links

Transgenic product class

missense_variant

(Vrailas-Mortimer et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Vrailas-Mortimer et al., 2011)

Mutagen

in vitro construct

(Vrailas-Mortimer et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-p38b.ala}

Cytology

Description

The critical Thr and Tyr residues of the TGY phosphorylation motif are mutated to Ala in this construct.

(Vrailas-Mortimer et al., 2014)

UAS regulatory sequences drive expression of a mutated form of p38b in which the threonine residue at 183 and the tyrosine residue at 185 have been replaced with alanine.

(Vrailas-Mortimer et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

p38b

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

(Vrailas-Mortimer et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^P0.5.Pdf

(Vrailas-Mortimer et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of p38b^ala.Scer\UAS in clock neurons using either Scer\GAL4^P0.5.Pdf or Scer\GAL4^cry.PZ results in a significant increase in arrhythmicity and tau under free-running conditions of 25.9h and 25.2h, respectively.

(Vrailas-Mortimer et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^ala.UAS has abnormal circadian rhythm phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^ala.UAS has abnormal neuroanatomy phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

NOT Suppressor of

Statement

Reference

p38b^ala.UAS/Scer\GAL4^Mef2.PR is a non-suppressor of partially lethal - majority die phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A per⁰¹ heterozygous background enhances the arrhythmicity and tau phenotype found upon expression of p38b^ala.Scer\UAS under the control of Scer\GAL4^cry.PZ.

(Vrailas-Mortimer et al., 2014)

Expression of p38b^ala.Scer\UAS in muscles under the control of Scer\GAL4^Mef2.PR does not suppress the reduction in viability seen in p38b^Δ25 Mpk2¹ double mutants.

(Vrailas-Mortimer et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p38b^ala.Scer\UAS

p38b^ala.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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