FlyBase Gene Report: Dmel\Rala

General Information

Symbol

Dmel\Rala

Species

D. melanogaster

Name

Ras-like protein A

Annotation Symbol

CG2849

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015286

Gene Model Status

Current

Stock Availability

22 publicly available

Gene Summary

Ras-like protein A (Rala) encodes a GTPase that regulates Notch, Jak/Stat and JNK signalling pathways. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

Ral, dRal, Ras-related protein, l(1)G0174, l(1)G0501

Key Links

Genomic Location

Cytogenetic map

3E5-3E6

Sequence location

X:3,704,610..3,719,635 [-]

Recombination map

1-4

RefSeq locus

NC_004354 REGION:3704610..3719635

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables GTPase activity

inferred from direct assay

(Sawamoto et al., 1999)

enables PDZ domain binding

inferred from physical interaction with FLYBASE:Magi; FB:FBgn0034590

(Beller et al., 2002)

enables protein binding

inferred from physical interaction with FLYBASE:Rlip; FB:FBgn0026056

(Mirey et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225, InterPro:IPR020849

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from electronic annotation with InterPro:IPR001806

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in border follicle cell migration

inferred from mutant phenotype

(Ghiglione et al., 2008)

involved_in defense response to Gram-negative bacterium

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

involved_in innate immune response

inferred from direct assay

(Chien et al., 2006)

involved_in negative regulation of innate immune response

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

involved_in negative regulation of JNK cascade

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(Balakireva et al., 2006, Sawamoto et al., 1999)

inferred from genetic interaction with FLYBASE:Jra; FB:FBgn0001291

(Balakireva et al., 2006)

inferred from genetic interaction with FLYBASE:kay; FB:FBgn0001297

(Balakireva et al., 2006)

inferred from genetic interaction with FLYBASE:msn; FB:FBgn0010909

(Balakireva et al., 2006)

inferred from genetic interaction with FLYBASE:Traf4; FB:FBgn0026319

(Balakireva et al., 2006)

inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303

(Sawamoto et al., 1999)

involved_in positive regulation of epidermal growth factor receptor signaling pathway

inferred from mutant phenotype

(Nászai et al., 2021)

involved_in positive regulation of stem cell proliferation

inferred from mutant phenotype

(Nászai et al., 2021)

involved_in R3/R4 cell fate commitment

inferred from mutant phenotype

(Cho and Fischer, 2011)

involved_in Ras protein signal transduction

inferred from genetic interaction with FLYBASE:Rgl; FB:FBgn0026376

(Mirey et al., 2003)

involved_in receptor internalization

inferred from mutant phenotype

(Nászai et al., 2021)

involved_in regulation of cell morphogenesis

inferred from mutant phenotype

(Sawamoto et al., 1999)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in Ras protein signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN008604616

(GO Reference Genome Project, 2011-)

involved_in signal transduction

inferred from electronic annotation with InterPro:IPR020849

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Ghiglione et al., 2008)

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR020849

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RAS GTPASES

Pathway (FlyBase)

POSITIVE REGULATORS OF EGFR SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the small GTPase superfamily. Ras family. (P48555)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Ras-like protein A (Rala) encodes a GTPase that regulates Notch, Jak/Stat and JNK signalling pathways. [Date last reviewed: 2019-08-01]

Gene Group (FlyBase)

RAS GTPASES -: The Ras family are members of the Ras superfamily of small GTPases. Ras proteins act in signal transduction cascades, generally the activated GTPase recruits and activates downstream effectors. Members this family are typically membrane-associated via isoprenylation of a CaaX motif in the C-terminus. (Adapted from PMID:15731001).

Pathway (FlyBase)

POSITIVE REGULATORS OF EGFR SIGNALING PATHWAY -: Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.

Summary (Interactive Fly)

Ras-like GTPase - counters apoptotic programs to support cell fate determination by acting as a negative regulator of JNK activity and a positive activator of p38 MAP kinase

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rala for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P48555)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

5CM8
5CM9

Comments on Gene Model

Due to significant sequence change in this region between Releases 4 and 5, data that predate Release 5 are not shown for some categories of evidence.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rala-RA

FBtr0070583

NM_166986

2377

201

Rala-RB

FBtr0070582

NM_166985

2057

197

Rala-RC

FBtr0070584

NM_080324

2555

201

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rala-PA

FBpp0070558

22.9

201

7.82

FBpp0070557

22.4

197

5.42

FBpp0070559

22.9

201

7.82

P48555

NP_525063

AAF45890

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

201 aa isoforms: Rala-PA, Rala-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

5CM8
5CM9

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rala using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rala_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.29

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 17

organism | ubiquitous

Comment: expressed in almost all cells

(Sawamoto et al., 1999)

third instar larval stage

larval brain

(Sawamoto et al., 1999)

larval ventral nerve cord | faint

(Sawamoto et al., 1999)

wing disc

(Sawamoto et al., 1999)

leg disc

(Sawamoto et al., 1999)

eye-antennal disc

(Sawamoto et al., 1999)

oogenesis stage S9

egg chamber

(Ghiglione et al., 2008)

border follicle cell

(Ghiglione et al., 2008)

polar follicle cell

(Ghiglione et al., 2008)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

eye disc posterior to the morphogenetic furrow

(Cho and Fischer, 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Ghiglione et al., 2008)

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

Reporter: P{GawB}Rala^PG69

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval salivary gland

(Cho and Fischer, 2011)

embryonic/larval anterior midgut

(Cho and Fischer, 2011)

embryonic/larval middle midgut

(Cho and Fischer, 2011)

photoreceptor cell R3 | precursor

(Cho and Fischer, 2011)

photoreceptor cell R4 | precursor

(Cho and Fischer, 2011)

Reporter: P{lacW}Rala^PL56#1

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S9

border follicle cell

(Ghiglione et al., 2008)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rala in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rala

View exonic expression by tissue for Dmel\Rala

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015286

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31332/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015286

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015286

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015286

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2849

Flygut - An atlas of the Drosophila adult midgut

FBgn0015286

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Rala

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 23 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 23 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rala

Transgenic constructs containing regulatory region of Rala

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene duplicated in

Dp(1;3)w⁺67k

(Eun et al., 2007)

Tp(1;2)w-ec

(Eun et al., 2007)

Gene disrupted in

Df(1)Exel6233

(Eun et al., 2007)

Df(1)N-264-105

(Eun et al., 2007)

Df(1)RR62

(Eun et al., 2007)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^unspecified

Rala^G20V.UAS

lethal | pharate adult stage

Rala^G0138

lethal | pupal stage

Rala^G0138

lethal | recessive

lethal - all die before end of larval stage

lethal - all die before end of P-stage

Rala^G0501

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Rala^GD8562

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rala^GD8562

viable, with Scer\GAL4^da.G32

Rala^S25N.cMa.UAS

viable, with Scer\GAL4^Mef2.PR

Rala^GD8562

viable, with Scer\GAL4^tin.CΔ4

Rala^GD8562

Sterility

Allele

male sterile

Rala^EE1

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^elav-C155

abnormal flight | male

Rala^EE1

abnormal planar polarity

Rala^EE1

abnormal planar polarity | somatic clone

Rala^EE1

abnormal size | adult stage, with Scer\GAL4^nub.PU

Rala^GD8562

abnormal size | adult stage, with Scer\GAL4^salm.EPv

Rala^GD8562

decreased cell size | somatic clone, with Scer\GAL4^Act5C.PP

Rala^G20V.UAS

decreased size | adult stage, with Scer\GAL4^Bx-MS1096

Rala^S25N.UAS

increased cell death | somatic clone

Rala^PG69

some die during larval stage

some die during pharate adult stage

Rala^G0501

some die during P-stage

Rala^G0501

some die during pupal stage, with Scer\GAL4^pnr-MD237

Rala^GD8562

visible

Rala^EE1

visible, with Scer\GAL4^69B

Rala^S25N.UAS

visible, with Scer\GAL4^sca-537.4

visible, with Scer\GAL4^sca.PU

visible | adult stage, with Scer\GAL4^Bx-MS1096

Rala^S25N.UAS

visible | recessive

Phenotype manifest in

Allele

adult head bristle

Rala^EE1

adult head bristle, with Scer\GAL4^sca.PU

Rala^S25N.cMa.UAS

border follicle cell, with Scer\GAL4^slbo.2.6, Scer\GAL4^upd1.PU

Rala^S25N.UAS

border follicle cell | somatic clone

Rala^PG69

chaeta

Rala^G0138

chaeta & mesothoracic tergum

Rala^EE1

crossvein, with Scer\GAL4^en-e16E

Rala^G20V.UAS

crossvein | increased number

Rala^S25N.UAS

embryo | dorsal closure stage, with Scer\GAL4^unspecified

Rala^G20V.UAS

embryonic/first instar larval cuticle, with Scer\GAL4^unspecified

Rala^G20V.UAS

embryonic/larval salivary gland

Rala^G0501

embryonic epidermis | anterior, with Scer\GAL4^LE

Rala^G20V.UAS

embryonic epidermis | anterior, with Scer\GAL4^pnr-MD237

Rala^G20V.UAS

embryonic epidermis | dorsal, with Scer\GAL4^LE

Rala^G20V.UAS

embryonic epidermis | dorsal, with Scer\GAL4^pnr-MD237

Rala^G20V.UAS

eye

eye photoreceptor cell

Rala^EE1

humeral bristle

Rala^EE1

macrochaeta

macrochaeta, with Scer\GAL4^sca-537.4

macrochaeta, with Scer\GAL4^sca.PU

microchaeta

microchaeta, with Scer\GAL4^sca-537.4

Rala^S25N.cMa.UAS

microchaeta, with Scer\GAL4^sca.PU

Rala^S25N.cMa.UAS

ommatidial precursor cluster

Rala^EE1

ommatidium

Rala^EE1

ommatidium | somatic clone

Rala^EE1

scutum & macrochaeta, with Scer\GAL4^69B

Rala^S25N.UAS

scutum & macrochaeta, with Scer\GAL4^sca-537.4

Rala^S25N.UAS

scutum & macrochaeta & tormogen cell, with Scer\GAL4^sca-537.4

Rala^S25N.UAS

scutum & macrochaeta & trichogen cell, with Scer\GAL4^sca-537.4

Rala^S25N.UAS

scutum & microchaeta, with Scer\GAL4^sca-537.4

Rala^S25N.UAS

scutum & microchaeta & tormogen cell, with Scer\GAL4^sca-537.4

Rala^S25N.UAS

scutum & microchaeta & trichogen cell, with Scer\GAL4^sca-537.4

Rala^S25N.UAS

scutum & trichome, with Scer\GAL4^69B

Rala^S25N.UAS

trichogen cell

trichogen cell, with Scer\GAL4^sca-537.4

Rala^S25N.cMa.UAS

wing, with Scer\GAL4^Bx-MS1096

Rala^S25N.UAS

wing, with Scer\GAL4^nub.PU

Rala^GD8562

wing, with Scer\GAL4^salm.EPv

Rala^GD8562

wing blade

Rala^EE1

wing hair, with Scer\GAL4^69B

Rala^S25N.UAS

wing vein

Rala^G0138

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (67)

Hsap\RALA

12 of 14

Yes

Hsap\RALB

11 of 14

Yes

Hsap\HRAS

3 of 14

Hsap\KRAS

3 of 14

Hsap\MRAS

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (44)

Rnor\Rala

12 of 14

Yes

Rnor\Ralb

10 of 14

Yes

Rnor\Eras

3 of 14

Rnor\Hras

3 of 14

Rnor\Mras

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (46)

Mmus\Rala

12 of 14

Yes

Mmus\Ralb

9 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (44)

Xtro\rala

7 of 13

Yes

Xtro\ralb

4 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (58)

Drer\ralaa

12 of 14

Yes

Drer\ralab

11 of 14

Yes

Drer\ralba

11 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\mrasb

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Drer\si:dkeyp-59c12.1

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (33)

Cele\ral-1

12 of 14

Yes

Cele\let-60

3 of 14

Cele\ras-2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\D1081.12

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (29)

Agam\AgaP_AGAP012108

11 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (36)

Atha\ARA4

1 of 13

Yes

Atha\ARA6

1 of 13

Yes

Atha\ARA7

1 of 13

Yes

Atha\AT5G59840

1 of 13

Yes

Atha\ATFP8

1 of 13

Yes

Atha\GB2

1 of 13

Yes

Atha\RA-5

1 of 13

Yes

Atha\RAB1A

1 of 13

Yes

Atha\RAB1C

1 of 13

Yes

Atha\RAB6A

1 of 13

Yes

Atha\RAB7A

1 of 13

Yes

Atha\RAB7B

1 of 13

Yes

Atha\RAB8

1 of 13

Yes

Atha\RAB11c

1 of 13

Yes

Atha\RAB18

1 of 13

Yes

Atha\RABA1d

1 of 13

Yes

Atha\RABA1g

1 of 13

Yes

Atha\RABA1i

1 of 13

Yes

Atha\RABA2b

1 of 13

Yes

Atha\RABA2c

1 of 13

Yes

Atha\RABA2D

1 of 13

Yes

Atha\RABA5d

1 of 13

Yes

Atha\RABA5E

1 of 13

Yes

Atha\RABB1a

1 of 13

Yes

Atha\RABC2A

1 of 13

Yes

Atha\RABE1c

1 of 13

Yes

Atha\RABE1e

1 of 13

Yes

Atha\RABG1

1 of 13

Yes

Atha\RABG3A

1 of 13

Yes

Atha\RABG3B

1 of 13

Yes

Atha\RABG3d

1 of 13

Yes

Atha\RABG3E

1 of 13

Yes

Atha\RABH1c

1 of 13

Yes

Atha\RABH1d

1 of 13

Yes

Atha\RABH1e

1 of 13

Yes

Atha\RHA1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\RAS1

2 of 13

Yes

Scer\RAS2

2 of 13

Yes

Scer\RHB1

2 of 13

Yes

Scer\RSR1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\ras1

2 of 12

Yes

Spom\rhb1

2 of 12

Yes

Spom\ypt2

1 of 12

Spom\ypt7

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rala. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (28)

Ras64B

5 of 13

Ras85D

5 of 13

Ric

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MRAS; muscle RAS oncogene homolog

model of Noonan syndrome 11

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

Rala^HMS01365

ameliorates amyotrophic lateral sclerosis type 10

modeled by TBPH^N493D.UAS

(Kankel et al., 2020)

ameliorates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^{R521C.UAS.Tag:HA}

(Kankel et al., 2020)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 4

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Rala^UAS.cRa

exacerbates intestinal cancer

modeled by Egfr^UAS.cBa

(Nászai et al., 2021)

modeled by Egfr^2.A887T.UAS

(Nászai et al., 2021)

Rala^S25N.UAS

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 4

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Rala^KK108989

ameliorates intestinal cancer

modeled by Egfr^UAS.cBa

(Nászai et al., 2021)

modeled by btl::Egfr^λ.UAS

(Nászai et al., 2021)

modeled by Egfr^2.A887T.UAS

(Nászai et al., 2021)

modeled by Src64B^UAS.cCa

(Nászai et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RALA; RAS like proto-oncogene A

12 of 14

179550

HIATT-NEU-COOPER NEURODEVELOPMENTAL SYNDROME; HINCONS

RALB; RAS like proto-oncogene B

11 of 14

179551

HRAS; HRas proto-oncogene, GTPase

3 of 14

HRAS PROTOONCOGENE, GTPase; HRAS

MRAS; muscle RAS oncogene homolog

3 of 14

608435

NOONAN SYNDROME 11; NS11

Noonan syndrome 11

NRAS; NRAS proto-oncogene, GTPase

3 of 14

164790

RIT1; Ras like without CAAX 1

3 of 14

609591

NOONAN SYNDROME 8; NS8

Noonan syndrome 8

RIT2; Ras like without CAAX 2

3 of 14

609592

RRAS2; RAS related 2

3 of 14

NOONAN SYNDROME 12; NS12

Noonan syndrome 12

RRAS; RAS related

3 of 14

165090

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rala - GEFmeso

pull down, autoradiography, two hybrid

(Blanke and Jackle, 2006)

Rala - Magi

two hybrid, pull down, autoradiography

(Beller et al., 2002)

Rala - Rlip

anti bait coimmunoprecipitation, Identification by mass spectrometry, two hybrid

(Brown et al., 2024, Blanke and Jackle, 2006, Mirey et al., 2003, Beller et al., 2002, Naitza et al., 2002)

Rala - Sec5

two hybrid

(Mirey et al., 2003)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rala

suppressible

bsk

(Balakireva et al., 2006, Sawamoto et al., 1999)

Rala

enhanceable

Delta

(Cho and Fischer, 2011)

Rala

suppressible

Dronc

(Balakireva et al., 2006)

Rala

enhanceable

Exo70

(Balakireva et al., 2006)

Rala

suppressible

hep

(Sawamoto et al., 1999)

Rala

suppressible

Jra

(Balakireva et al., 2006)

Rala

suppressible

kay

(Balakireva et al., 2006)

Rala

suppressible

lqf

(Cho and Fischer, 2011)

Rala

enhanceable

Mekk1

(Balakireva et al., 2006)

Rala

enhanceable

mnb

(Brown et al., 2024)

Rala

suppressible

msn

(Balakireva et al., 2006)

Rala

enhanceable

msn

(Balakireva et al., 2006)

Rala

enhanceable

N::sev

(Cho and Fischer, 2011)

Rala

enhanceable

(Cho and Fischer, 2011)

Rala

enhanceable

neur

(Cho and Fischer, 2011)

Rala

enhanceable

p38b

(Balakireva et al., 2006)

Rala

suppressible

p38b

(Balakireva et al., 2006)

Rala

enhanceable

puc

(Balakireva et al., 2006)

Rala

suppressible

puc

(Balakireva et al., 2006)

Rala

suppressible

Rap1

(Mirey et al., 2003)

Rala

enhanceable

Ras85D

(Mirey et al., 2003)

Ras85D|Rala

suppressible

Rgl

(Mirey et al., 2003)

Rala

suppressible

Rgl

(Mirey et al., 2003)

Rala

suppressible

Rlip

(Balakireva et al., 2006)

Rala

enhanceable

Rlip

(Balakireva et al., 2006)

Rala

enhanceable

Sec5

(Balakireva et al., 2006)

Rala

enhanceable

Sec15

(Balakireva et al., 2006)

Rala

enhanceable

Traf4

(Balakireva et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Itpr

suppressible

Rala

(Richhariya et al., 2017)

lqf

suppressible

Rala

(Cho and Fischer, 2011)

enhanceable

(Eun et al., 2007)

enhanceable

(Brown et al., 2024)

Orai

suppressible

Rala

(Richhariya et al., 2017)

Rap1

suppressible

Rala

(Mirey et al., 2003)

Ras85D

enhanceable

Rala

(Mirey et al., 2003)

Smox

suppressible

Rala

(Hevia et al., 2017)

Stim

suppressible

Rala

(Richhariya et al., 2017)

enhanceable

(He et al., 2005)

suppressible

(He et al., 2005)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

57853

DroID - A comprehensive database of gene and protein interactions.

FBgn0015286

MIST (genetic) - An integrated Molecular Interaction Database

31332

MIST (protein-protein) - An integrated Molecular Interaction Database

31332

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF EGFR SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-4

Cytogenetic map

3E5-3E6

Sequence location

X:3,704,610..3,719,635 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3E5-3E6

Limits computationally determined from genome sequence between P{EP}EP1623 and P{EP}EP911&P{EP}EP1094

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

3E-3E

(determined by in situ hybridisation) 3E1--4 (determined by in situ hybridisation) 3E5--F2 (determined by in situ hybridisation) 3E5--8 (determined by in situ hybridisation)

(Peter et al., 2002)

3E5-3F2

(determined by in situ hybridisation)

(Schäfer et al., 1999.12.2)

3E-3E

(determined by in situ hybridisation)

(Sawamoto et al., 1999, Schäfer et al., 1999.6.15)

3E1-3E4

(determined by in situ hybridisation)

(Schäfer et al., 1999.8.12)

3E-3E

(Camonis, 1999.4.6)

Experimentally Determined Recombination Data

Location

1-4

(FlyBase, 2016)

1-4.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (22)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

25095

Rala^EE1/FM7c

606824

w^*; P{UAS-Rala.HA}2

32094

w^*; P{UAS-Rala.S25N}2

81049

w^*; P{UAS-Rala.G20V}3

606825

w^*; P{UAS-Rala.GFP.S}3

29580

y¹ v¹; P{TRiP.JF03259}attP2

12283

w^67c23 P{lacW}Rala^G0501/FM7c

81048

w^*; P{UAS-Rala.S}3/TM6B, Tb¹

11334

2849R-1

v105296

v43622

w¹¹¹⁸; P{GD8562}v43622

More stocks available...

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (151)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD21679

Other clones

DPiM_LD21679

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015286

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015286

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Teodoro et al., 2013, Ghiglione et al., 2008)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Rala represses ligand-independent activation of N in the developing R3 photoreceptor cell.

(Cho and Fischer, 2011)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Rala regulates developmental cell shape changes through the JNK pathway.

(Sawamoto et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: Rala anon- EST:fe1H4

(FlyBase, 1996-)

Source for merge of: Rala l(1)G0174 l(1)G0373 l(1)G0501

(Peter et al., 2002)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

24639550

(Rojas et al., 2012)

CG2849

(Hevia et al., 2017, Hosono et al., 2015, Christis and Munro, 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Ghiglione et al., 2008, Gandhi et al., 2007, Larschan et al., 2007, Stuart et al., 2007, Walker et al., 2006, Colicelli, 2004, Bourbon et al., 2002)

D-RalA

(Ryabinina et al., 2006)

DRal

(Blanke and Jackle, 2006, Blanke et al., 2001, Sawamoto et al., 1999)

DRala

(Chen et al., 2002)

Dm RalA

(Jekely, 2003)

Dral

(He et al., 2005)

DralA

(Ghiglione et al., 2008)

Drala

(He and Adler, 2003)

P48555

(Rojas et al., 2012)

RALA

(Winge, 1995.3.30)

Ral

(Richhariya and Hasan, 2020, Xu et al., 2020, Richhariya et al., 2017, Lee and Schwarz, 2016, Sundaram, 2015, Teodoro et al., 2013, Kelsom and Lu, 2012, Cho and Fischer, 2011, Balakireva et al., 2007, Beraud et al., 2007, Lavery et al., 2007, Balakireva et al., 2006, Chien et al., 2006, Gagic and Müller, 2004, Geisbrecht and Montell, 2004, Balakireva et al., 2003, Mirey et al., 2003, Moskalenko et al., 2002, de Bruyn et al., 2000)

Ral-a

(Bourbon et al., 2002)

RalA

(Sanal et al., 2023, Boumard and Bardin, 2021, Richhariya and Hasan, 2020, Johansson et al., 2019, Holly et al., 2015, Ghiglione et al., 2008, Dupuy et al., 2005, Jasper et al., 2001)

anon-EST:fe1H4

dRal

(Popovic et al., 2016, Brentrup and Jackle, 2003, Beller et al., 2002, Beller et al., 2002, Beller et al., 2002, Naitza et al., 2002, Prober and Edgar, 2002, Camonis, 1999.4.6, Mirey et al., 1999, Mirey et al., 1999)

l(1)G0174

(Schäfer et al., 1999.6.15)

l(1)G0373

(Schäfer et al., 1999.8.12)

l(1)G0501

(Schäfer et al., 1999.12.2)

ral

(Tracy et al., 2016, Balakireva et al., 2007, Gagic et al., 2005)

ralA

(Thukral et al., 2022, Koon et al., 2018, Loo et al., 2005)

rala

(Megha and Hasan, 2020)

Name Synonyms

Ras-like Protein A

(Palmer and Deng, 2015)

Ras-like protein A

(Carnes et al., 2015)

Ras-related protein

(Shen et al., 2013, Cammarato et al., 2011, Sambandan et al., 2006)

anon-fast-evolving-1H4

Secondary FlyBase IDs

FBgn0025241
FBgn0027285
FBgn0028308
FBgn0040134

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31332

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P48555

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P48555

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q962I2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P48555

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015286

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31332/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015286

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015286

FlyMine - An integrated database for Drosophila genomics

FBgn0015286

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2849

MARRVEL_MODEL - MARRVEL (model organism gene)

31332

PDB - An information portal to biological macromolecular structures

5CM8
5CM9

Linkouts

BioGRID - A database of protein and genetic interactions.

57853

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015286

DroID - A comprehensive database of gene and protein interactions.

FBgn0015286

DRSC - Results frm RNAi screens

FBgn0015286

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rala_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015286

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015286

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2849

Flygut - An atlas of the Drosophila adult midgut

FBgn0015286

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012218

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

31332

MIST (protein-protein) - An integrated Molecular Interaction Database

31332

References (168)

The following external sites may use different assemblies or annotations than FlyBase.

-1.29

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