FlyBase Gene Report: Dmel\trc

General Information

Symbol

Dmel\trc

Species

D. melanogaster

Name

tricornered

Annotation Symbol

CG8637

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003744

Gene Model Status

Current

Stock Availability

28 publicly available

Gene Summary

tricornered (trc) encodes a nuclear Dbf2-related kinase whose mutations lead to mulitpled hairs, split and multipled arista laterals and to defects in dendritic tiling. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Ndr, tricorner

Key Links

Genomic Location

Cytogenetic map

76D1-76D1

Sequence location

3L:19,830,864..19,834,304 [-]

Recombination map

3-46

RefSeq locus

NT_037436 REGION:19830864..19834304

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (27 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:fry; FB:FBgn0016081

(He et al., 2005)

inferred from physical interaction with UniProtKB:Q8IQG1

(He et al., 2005)

enables protein serine/threonine kinase activity

inferred from direct assay

(Emoto et al., 2004)

inferred from mutant phenotype

(Norkett et al., 2020)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from sequence or structural similarity with UniProtKB:Q15208

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR017441, InterPro:IPR017892

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from electronic annotation with InterPro:IPR000961, InterPro:IPR017892

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q15208

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000683254

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q15208

(Millward et al., 1995)

Biological Process (15 terms)

Terms Based on Experimental Evidence (14 terms)

CV Term

Evidence

References

involved_in antennal morphogenesis

inferred from mutant phenotype

(He and Adler, 2002)

involved_in chaeta development

inferred from mutant phenotype

(He et al., 2005)

involved_in dendrite morphogenesis

inferred from mutant phenotype

(Grueber et al., 2007)

involved_in dendrite self-avoidance

inferred from genetic interaction with FLYBASE:stan; FB:FBgn0024836

(Matsubara et al., 2011)

involved_in imaginal disc-derived wing hair organization

inferred from mutant phenotype

(He et al., 2005, Cong et al., 2001)

involved_in imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Cong et al., 2001)

involved_in intracellular signal transduction

inferred from direct assay

(Emoto et al., 2004)

involved_in microtubule sliding

inferred from mutant phenotype

(Norkett et al., 2020)

involved_in negative regulation of neuron projection arborization

inferred from mutant phenotype

(Norkett et al., 2020)

involved_in peptidyl-serine phosphorylation

inferred from mutant phenotype

(Norkett et al., 2020)

involved_in protein phosphorylation

inferred from direct assay

(Emoto et al., 2004)

involved_in regulation of dendrite development

inferred from mutant phenotype

(Emoto et al., 2004)

involved_in regulation of dendrite morphogenesis

inferred from mutant phenotype

(Emoto et al., 2004)

involved_in signal transduction

inferred from mutant phenotype

(He et al., 2005, Emoto et al., 2004)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in establishment or maintenance of cell polarity

inferred from biological aspect of ancestor with PANTHER:PTN000684318

(GO Reference Genome Project, 2011-)

involved_in intracellular signal transduction

inferred from sequence or structural similarity with UniProtKB:Q15208

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000683254

(GO Reference Genome Project, 2011-)

involved_in protein phosphorylation

inferred from sequence or structural similarity with UniProtKB:Q15208

(Gene Ontology Curators, 2002-)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Emoto et al., 2004)

located_in cell body

inferred from direct assay

(Emoto et al., 2004)

located_in cell cortex

inferred from direct assay

(He et al., 2005)

located_in cell hair

inferred from direct assay

(He et al., 2005)

located_in cell periphery

inferred from direct assay

(He et al., 2005)

located_in cytoplasm

inferred from direct assay

(Horne-Badovinac et al., 2012, He et al., 2005)

inferred from direct assay

(He et al., 2005)

located_in dendrite

inferred from direct assay

(Emoto et al., 2004)

located_in nucleus

inferred from direct assay

(Horne-Badovinac et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from sequence or structural similarity with UniProtKB:Q15208

(Gene Ontology Curators, 2002-)

inferred from sequence or structural similarity with UniProtKB:Q15208

(Millward et al., 1995)

Gene Group (FlyBase)

UNCLASSIFIED AGC KINASES

Protein Family (UniProt)

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. (Q9NBK5)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED AGC KINASES -: This group comprises AGC kinases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

Serine/threonine-protein kinase involved in controlling cell structure and proliferation of a variety of polarized outgrowths including epidermal hairs, bristles, arista laterals, and dendrites (PubMed:11102376, PubMed:15479641, PubMed:32022690). Together with fry, maintains the integrity of epidermal hairs and is an essential component of the signaling pathway regulating dendritic branching of sensory neurons (PubMed:15479641). Reduces neurite outgrowth by phosphorylating pav, thereby inhibiting its function in microtubule-microtubule sliding (PubMed:32022690).

(UniProt, Q9NBK5)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

trc: tricorner

Mutant wing and notum have rosettes of three or more short trichomes instead of single long hairs as in wild type. Useful as cell marker. Mutant cell autonomous in mitotic cells in a wild-type background, but not in a Minute background (Vinson and Adler, 1987a).

Summary (Interactive Fly)

Ndr serine/threonine kinase - required for the normal morphogenesis of epidermal hairs, bristles, laterals, and dendrites - regulates synapse development by regulating the levels of Wiskott-Aldrich Syndrome protein

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\trc for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9NBK5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

trc-RA

FBtr0074922

NM_079446

2277

459

trc-RB

FBtr0332154

NM_001275142

2816

463

Additional Transcript Data and Comments

Reported size (kB)

2.1 (longest cDNA)

(Millward et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

trc-PA

FBpp0074691

52.9

459

6.37

NP_524170

AAF49104

trc-PB

FBpp0304464

53.3

463

6.37

Q9NBK5

NP_001262071

AGB94764

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

455 (aa); 52 (kD predicted)

(Millward et al., 1995)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with, and is activated by, Mob1.

(UniProt, Q9NBK5)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\trc using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

trc_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.59

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S6 -- S8 & adult stage | female

follicle cell

(Horne-Badovinac et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Emoto et al., 2004)

located_in cell body

inferred from direct assay

(Emoto et al., 2004)

located_in cell cortex

inferred from direct assay

(He et al., 2005)

located_in cell hair

inferred from direct assay

(He et al., 2005)

located_in cell periphery

inferred from direct assay

(He et al., 2005)

located_in cytoplasm

inferred from direct assay

(Horne-Badovinac et al., 2012, He et al., 2005)

inferred from direct assay

(He et al., 2005)

located_in dendrite

inferred from direct assay

(Emoto et al., 2004)

located_in nucleus

inferred from direct assay

(Horne-Badovinac et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\trc in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\trc

View exonic expression by tissue for Dmel\trc

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003744

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40165/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003744

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003744

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003744

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8637

Flygut - An atlas of the Drosophila adult midgut

FBgn0003744

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of trc

Transgenic constructs containing regulatory region of trc

Aberrations (Deficiencies and Duplications) ( 28 )

Inferred from experimentation ( 28 )

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

trc^S066917a

lethal | recessive

trc^S066917a

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

trc^GD13993

lethal - all die during embryonic stage | recessive

trc^S066917a

viable, with Scer\GAL4^Mef2.PR

trc^GD13993

viable (with Df(3L)kto2)

trc⁸

Sterility

Allele

female fertile, with Scer\GAL4^nanos.PG

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

trc^GL00028

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

trc^GL01127

Other Phenotypes

Allele

abnormal cell shape | somatic clone

trc^S066917a

abnormal developmental rate | pupal stage (with trc¹)

trc⁸

abnormal developmental rate | pupal stage (with trc⁸)

trc¹

abnormal neuroanatomy

trc^unspecified

abnormal neuroanatomy, with Scer\GAL4^21-7

trc^KK109419

abnormal neuroanatomy | third instar larval stage (with Df(3L)BSC445)

trc¹

abnormal planar polarity (with Df(3L)kto2)

trc⁸

abnormal planar polarity | somatic clone

trc¹

decreased body size | adult stage, with Scer\GAL4^Ilp2.PR

some die during embryonic stage | recessive

trc^S066917a

some die during pupal stage, with Scer\GAL4^pnr-MD237

trc^GD13993

visible

visible, with Scer\GAL4^ap-md544

trc^T453A.UAS.L

visible, with Scer\GAL4^ptc-559.1

trc^T453A.UAS.L

visible (with Df(3L)kto2)

trc⁸

visible | recessive | somatic clone

trc^S066917a

visible | somatic clone

visible | somatic clone (with trc¹)

trc⁸

visible | somatic clone (with trc⁸)

trc¹

Phenotype manifest in

Allele

actin filament | somatic clone

trc^K23

adult abdominal sensillum (with Df(3L)kto2)

trc⁸

antenna (with trc¹)

trc⁸

antenna (with trc⁸)

trc¹

arista

arista lateral (with trc¹)

trc⁸

arista lateral (with trc⁸)

trc¹

chaeta

trc¹

dendrite

trc^unspecified

dendrite, with Scer\GAL4^21-7

trc^KK109419

dendrite | cell autonomous | somatic clone | increased number

trc¹

dendritic arborizing neuron

trc^unspecified

denticle

trc^S066917a

denticle (with Df(3L)kto2)

trc¹

egg | somatic clone

egg chamber | somatic clone

trc^K23

embryo | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

trc^GL01127

embryonic/larval dorsal tracheal branch | larval stage, with Scer\GAL4^drm.7.1

trc^GL01127

follicle cell | somatic clone

trc^K23

larval dorsal multidendritic neuron ddaC | third instar larval stage (with Df(3L)BSC445)

trc¹

larval multidendritic class IV neuron, with Scer\GAL4^21-7

trc^KK109419

macrochaeta, with Scer\GAL4^ap-md544

macrochaeta, with Scer\GAL4^neur-P72

macrochaeta (with Df(3L)kto2)

trc⁸

multidendritic neuron & dendrite

trc¹

multidendritic neuron & dendrite, with Scer\GAL4⁴⁷⁷

multidendritic neuron & dendrite | somatic clone

trc¹

multidendritic neuron & dendrite | supernumerary

trc¹

multidendritic neuron & dendrite | supernumerary, with Scer\GAL4⁴⁷⁷

terminal tracheal cell | larval stage, with Scer\GAL4^drm.7.1

trc^GL01127

trichogen cell (with Df(3L)kto2)

trc⁸

trichome | increased number (with Df(3L)kto2)

trc⁸

ventral denticle belt (with Df(3L)kto2)

trc¹

wing (with Df(3L)kto2)

trc⁸

wing hair | increased number

trc^S066917a

wing hair | increased number, with Scer\GAL4^ap-md544

wing hair | increased number, with Scer\GAL4^ap-md544, trc^S292A.UAS.L

trc^S066917a

wing hair | increased number, with Scer\GAL4^ap-md544, trc^S066917a

trc^S292A.UAS.L

wing hair | increased number, with Scer\GAL4^ptc-559.1

trc^T453A.UAS.L

wing hair | increased number (with Df(3L)kto2)

trc⁸

wing hair | P-stage | cell autonomous | somatic clone | increased number

trc^S066917a

wing hair | somatic clone

trc¹

wing hair | somatic clone | increased number

wing hair | somatic clone | increased number (with trc¹)

trc⁸

wing hair | somatic clone | increased number (with trc⁸)

trc¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (49)

Hsap\STK38

13 of 14

Yes

Hsap\STK38L

11 of 14

Yes

Hsap\DMPK

3 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (27)

Rnor\Stk38l

11 of 14

Yes

Rnor\Stk38

11 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (27)

Mmus\Stk38

13 of 14

Yes

Mmus\Stk38l

11 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (28)

Xtro\stk38

8 of 13

Yes

Xtro\stk38l

6 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\mast3

1 of 13

Xtro\or5l1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\sgk3

1 of 13

Danio rerio (Zebrafish) (39)

Drer\stk38a

13 of 14

Yes

Drer\stk38b

11 of 14

Yes

Drer\stk38l

11 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC101883172

1 of 14

Yes

Drer\LOC101883269

1 of 14

Yes

Drer\LOC101884327

1 of 14

Yes

Drer\LOC101886143

1 of 14

Yes

Drer\LOC108182331

1 of 14

Yes

Drer\LOC110438182

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (20)

Cele\sax-1

11 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (19)

Agam\AgaP_AGAP005521

11 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (41)

Atha\AT5G09890

12 of 13

Yes

Atha\AT1G03920

11 of 13

Yes

Atha\AT1G30640

11 of 13

Yes

Atha\AT2G19400

11 of 13

Yes

Atha\AT2G20470

11 of 13

Yes

Atha\AT3G23310

11 of 13

Yes

Atha\AT4G14350

11 of 13

Yes

Atha\AT4G33080

11 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT1G48490

1 of 13

Atha\AT2G36350

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G03640

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\PHOT2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\UCNL

1 of 13

Yes

Atha\WAG1

1 of 13

Atha\WAG2

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (18)

Scer\CBK1

11 of 13

Yes

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\orb6

10 of 12

Yes

Spom\cek1

3 of 12

Yes

Spom\ppk18

3 of 12

Yes

Spom\ppk31

3 of 12

Yes

Spom\mug27

2 of 12

Yes

Spom\sid2

2 of 12

Yes

Spom\gad8

1 of 12

Spom\ppk14

1 of 12

Yes

Spom\ppk22

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:trc. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (22)

wts

4 of 13

Akt

2 of 13

aPKC

2 of 13

dop

2 of 13

gek

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Pkn

2 of 13

Rok

2 of 13

sti

2 of 13

CG32944

1 of 13

for

1 of 13

gwl

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

S6k

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

DMPK; DM1 protein kinase

model of myotonic dystrophy type 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

trc^L.UAS

ameliorates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

modeled by Pink1^B9

(Wu et al., 2013)

trc^S292E.UAS.L

ameliorates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

modeled by Pink1^B9

(Wu et al., 2013)

DOES NOT ameliorate Parkinson's disease

modeled by park¹, park^Δ21

(Wu et al., 2013)

trc^T453E.UAS.L

ameliorates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

trc^short.UAS

ameliorates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

trc^{K122A+T453A.UAS.L}

exacerbates Parkinson's disease

modeled by Pink1^B9

(Wu et al., 2013)

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

trc^{K122A.UAS.L.Tag:FLAG}

exacerbates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

STK38; serine/threonine kinase 38

13 of 14

606964

STK38L; serine/threonine kinase 38 like

11 of 14

615836

DMPK; DM1 protein kinase

3 of 14

DYSTROPHIA MYOTONICA PROTEIN KINASE; DMPK

MYOTONIC DYSTROPHY 1; DM1

myotonic dystrophy type 1

LATS1; large tumor suppressor kinase 1

3 of 14

LARGE TUMOR SUPPRESSOR KINASE 1; LATS1

LATS2; large tumor suppressor kinase 2

3 of 14

LARGE TUMOR SUPPRESSOR KINASE 2; LATS2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

trc

Hsap\STK38

(He et al., 2005)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

trc - Atg8a

two hybrid, pull down, anti tag western blot

(Tsapras et al., 2022)

trc - GckIII

enzymatic study, western blot

(Poon et al., 2018)

trc - Mob2

two hybrid, anti tag coimmunoprecipitation, western blot

(He et al., 2005)

trc - Rac1

anti bait coimmunoprecipitation, anti tag western blot

(Emoto et al., 2004)

trc - Sin1

anti tag coimmunoprecipitation, western blot

(Koike-Kumagai et al., 2009)

trc - fry

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Fang et al., 2010, He et al., 2005)

trc - hpo

enzymatic study, autoradiography, anti tag coimmunoprecipitation, western blot, anti tag western blot

(Emoto et al., 2006)

trc - mats

two hybrid

(He et al., 2005)

trc - raw

anti tag coimmunoprecipitation, anti tag western blot

(Lee et al., 2015)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Wu et al., 2013)

enhanceable

(Hudson et al., 2023)

trc

enhanceable

blw

(Hudson et al., 2023)

enhanceable

(He et al., 2005)

suppressible

(Hudson et al., 2023)

trc

enhanceable

Ccm3

(Hudson et al., 2023)

enhanceable

(He et al., 2005)

suppressible

(Hudson et al., 2023)

trc

suppressible

cora

(Hudson et al., 2023)

trc

enhanceable

Csas

(Hudson et al., 2023)

trc

enhanceable

CycT

(Hudson et al., 2023)

trc

suppressible

CycT

(Hudson et al., 2023)

trc

suppressible

eEF2

(Hudson et al., 2023)

trc

enhanceable

Fancm

(Hudson et al., 2023)

trc

enhanceable

fry

(Hudson et al., 2023, He et al., 2005)

enhanceable

(He et al., 2005)

enhanceable

(Hudson et al., 2023)

trc

suppressible

LamC

(Hudson et al., 2023)

suppressible

(Wu et al., 2013)

enhanceable

(He et al., 2005)

suppressible

(He et al., 2005)

enhanceable

(Hudson et al., 2023)

trc

enhanceable

Mip

(Hudson et al., 2023)

suppressible

(Wu et al., 2013)

enhanceable

(Hudson et al., 2023)

trc

enhanceable

Mob2

(Hudson et al., 2023, He et al., 2005)

trc

enhanceable

Nak

(Hudson et al., 2023)

trc

enhanceable

ND-75

(Hudson et al., 2023)

trc

enhanceable

Nurf-38

(Hudson et al., 2023)

trc

enhanceable

Pfk

(Hudson et al., 2023)

trc

enhanceable

Pyk

(Hudson et al., 2023)

enhanceable

(He et al., 2005)

suppressible

(Emoto et al., 2004)

enhanceable

(He et al., 2005)

suppressible

(He et al., 2005)

enhanceable

(He et al., 2005)

suppressible

(Hudson et al., 2023)

trc

enhanceable

Septin1

(Hudson et al., 2023)

trc

enhanceable

Su(Tpl)

(Hudson et al., 2023)

suppressible

(He et al., 2005)

enhanceable

(He et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hpo

suppressible

trc

(Emoto et al., 2006)

suppressible

(Wu et al., 2013)

enhanceable

(Wu et al., 2013)

suppressible

(Emoto et al., 2004)

suppressible

(Lee et al., 2015)

enhanceable

(Lee et al., 2015)

suppressible

(Koike-Kumagai et al., 2009)

Sema2b

enhanceable

trc

(Meltzer et al., 2016)

Sema2b

suppressible

trc

(Meltzer et al., 2016)

Sin1

suppressible

trc

(Koike-Kumagai et al., 2009)

ultA

enhanceable

trc

(Lee and Adler, 2002)

External Data

Subunit Structure (UniProtKB)

Interacts with, and is activated by, Mob1.

(UniProt, Q9NBK5 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65434

DroID - A comprehensive database of gene and protein interactions.

FBgn0003744

MIST (genetic) - An integrated Molecular Interaction Database

40165

MIST (protein-protein) - An integrated Molecular Interaction Database

40165

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9NBK5

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-46

Cytogenetic map

76D1-76D1

Sequence location

3L:19,830,864..19,834,304 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

76D1-76D1

Limits computationally determined from genome sequence between P{lacW}l(3)L3809^L3809 and P{lacW}l(3)L1243^L1243&P{lacW}Mi-2^j3D4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

76C-76C

(Adler, 2000.3.21, Geng, 2000.2.22)

76C4-76C6

(determined by in situ hybridisation)

(Szeged Stock Center, 1998-, Deak et al., 1997)

Experimentally Determined Recombination Data

Location

3-46

(FlyBase, 2016)

3-43.2

(Comeron et al., 2012)

3-48

(Martin, 1995.12.5)

Left of (cM)

Right of (cM)

Notes

Mitotic location: 36.

(Martin, 1995.12.5)

Stocks and Reagents

Stocks (28)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

32085

w^*; P{UAS-trc.L}3

32090

w^*; P{UAS-trc-GFP}2

32080

w^*; P{UAS-trc.short}3

32083

w^*; P{UAS-trc.L.T453A}2

32084

w^*; P{UAS-trc.L.S292A}2

32088

w^*; P{UAS-trc.L.T453A}3

32091

w^*; P{UAS-trc.L.S292E}3

32081

w^*; P{UAS-trc.L.S292E}2/CyO

8637R-1

8637R-2

v107923

v35988

w¹¹¹⁸; P{GD13993}v35988/CyO

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (188)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD37189

Other clones

DPiM_LD37189

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003744

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003744

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2015, Koike-Kumagai et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

trc is important for maintaining the integrity of outgrowths of epidermal cells.

(Geng et al., 2000)

Mutations in trc result in the splitting or branching of cuticular structures produced by cytoskeletal-mediated outgrowths of epidermal cells (the adult epidermal hairs, the shafts of adult sense organs, the lateral extensions of the arista and the larval denticles).

(Geng et al., 2000)

Mutants do not exhibit defects in the denticle belt of hairs of the larvae.

(Dickinson and Thatcher, 1997)

cDNA clone encoding the homologue of human Ndr has been isolated and sequenced.

(Millward et al., 1995)

Mutant alleles are useful as markers in clonal analysis.

(Lawrence et al., 1986)

Relationship to Other Genes

Source for database merge of

Source for merge of: trc l(3)S066917

(Geng et al., 2000)

Additional comments

Source for identity of trc CG8637 was sequence comparison ( date:000504 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: trc CG8637

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

0669/17

(Deak et al., 1997)

CG8637

(Hosono et al., 2015, Joffre et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Morrison et al., 2000)

NDR

(Morrison et al., 2000, Bassett et al., 1997)

Ndr

(Millward et al., 1995, Millward, 1994.7.6)

RE-D

(Horne-Badovinac et al., 2012)

dmTRC

(Tamaskovic et al., 2003)

l(3)76BDa

(Cooper et al., 2010)

l(3)S066917

trc

(Hudson et al., 2023, Beaver et al., 2022, Ribot et al., 2022, Tsapras and Nezis, 2022, Lebo et al., 2021, Ostalé et al., 2021, Port et al., 2020, Rui et al., 2020, Meltzer et al., 2019, Yang and Chien, 2019, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, Meltzer et al., 2016, Lee et al., 2015, Luck et al., 2014, Sopko et al., 2014, Campbell and Ganetzky, 2013, Czech et al., 2013, Gates, 2012, Han et al., 2012, Horne-Badovinac et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Chung et al., 2011, Hogan et al., 2011, Matsubara et al., 2011, Adler, 2010.9.10, Cooper et al., 2010, Fang and Adler, 2010, Fang et al., 2010, Koike-Kumagai et al., 2009, Long et al., 2009, Cukier et al., 2008, Beltran et al., 2007, Fang and Adler, 2007, Grueber et al., 2007, Soba et al., 2007, He et al., 2005, He et al., 2005, Zinn, 2004, Fei et al., 2002)

Name Synonyms

Nuclear Dbf2-related protein kinase

Tricornered

(Hergovich, 2017, Hergovich, 2016, Fang et al., 2010, Long et al., 2009, Emoto et al., 2006, He et al., 2005)

tricorner

(Adler, 2000.3.21, Lindsley and Zimm, 1992)

tricornered

(Gates, 2012, Hogan et al., 2011, Fang and Adler, 2010, Koike-Kumagai et al., 2009, Cukier et al., 2008, Fang and Adler, 2007, Chanut-Delalande et al., 2006, Edgar, 2006, Zinn, 2004, Fei et al., 2002)

Secondary FlyBase IDs

FBgn0014866
FBgn0028111

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40165

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9NBK5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9NBK5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PG43

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9NBK5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003744

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40165/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003744

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003744

FlyMine - An integrated database for Drosophila genomics

FBgn0003744

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8637

MARRVEL_MODEL - MARRVEL (model organism gene)

40165

Linkouts

BioGRID - A database of protein and genetic interactions.

65434

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003744

DroID - A comprehensive database of gene and protein interactions.

FBgn0003744

DRSC - Results frm RNAi screens

FBgn0003744

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

trc_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003744

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003744

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8637

Flygut - An atlas of the Drosophila adult midgut

FBgn0003744

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003744

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032646

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

40165

MIST (protein-protein) - An integrated Molecular Interaction Database

40165

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9NBK5

References (136)

The following external sites may use different assemblies or annotations than FlyBase.

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