FlyBase Gene Report: Dmel\Nak

General Information

Symbol

Dmel\Nak

Species

D. melanogaster

Name

Numb-associated kinase

Annotation Symbol

CG10637

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015772

Gene Model Status

Current

Stock Availability

11 publicly available

Enzyme Name (EC)

Gene Summary

Numb-associated kinase (Nak) encodes an Ark family member that regulates the product of numb function. It contributes to dendrite development by regulating clathrin localization. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

37B7-37B7

Sequence location

2L:18,957,732..18,968,744 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:18957732..18968744

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables AP-2 adaptor complex binding

inferred from direct assay

(Yang et al., 2011)

enables protein binding

inferred from physical interaction with FLYBASE:numb; FB:FBgn0002973

(Chien et al., 1998)

enables PTB domain binding

inferred from physical interaction with FLYBASE:numb; FB:FBgn0002973

(Zwahlen et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables AP-2 adaptor complex binding

inferred from biological aspect of ancestor with PANTHER:PTN001121691

(GO Reference Genome Project, 2011-)

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from sequence or structural similarity with UniProtKB:P38080

(Chien et al., 1998)

inferred from biological aspect of ancestor with PANTHER:PTN000539981

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in asymmetric cell division

inferred from mutant phenotype

(Chien et al., 1998)

inferred from physical interaction with FLYBASE:numb; FB:FBgn0002973

(Chien et al., 1998)

involved_in dendrite morphogenesis

inferred from mutant phenotype

(Yang et al., 2011)

involved_in positive regulation of Notch signaling pathway

inferred from direct assay

(Chien et al., 1998)

involved_in regulation of clathrin-dependent endocytosis

inferred from genetic interaction with FLYBASE:Chc; FB:FBgn0000319

(Yang et al., 2011)

inferred from genetic interaction with FLYBASE:AP-2α; FB:FBgn0264855

(Yang et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in positive regulation of Notch signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001121691

(GO Reference Genome Project, 2011-)

involved_in regulation of clathrin-dependent endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN001121691

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Yang et al., 2011)

located_in dendrite

inferred from direct assay

(Yang et al., 2011)

located_in neuronal cell body

inferred from direct assay

(Yang et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000539981

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES

Pathway (FlyBase)

POSITIVE REGULATORS OF NOTCH SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -: This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

POSITIVE REGULATORS OF NOTCH SIGNALING PATHWAY -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Summary (Interactive Fly)

A component of the endocytic pathway - regulates Neuroglian endocytosis during dendritic extension - regulates Discs large localization in salivary gland cells - antagonizes the function of Numb and consequently regulates asymmetric cell division

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nak for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VJ30)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nak-RA

FBtr0081187

NM_057817

5196

1488

Nak-RC

FBtr0081189

NM_206003

4161

698

Nak-RD

FBtr0340325

NM_057818

5364

1488

Nak-RE

FBtr0340326

NM_001299146

4280

708

Additional Transcript Data and Comments

Reported size (kB)

5.5, 4.5, 3.0 (northern blot)

(Chien et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nak-PA

FBpp0080729

159.7

1488

5.98

FBpp0089380

74.7

698

6.99

FBpp0309286

159.7

1488

5.98

FBpp0309287

75.9

708

6.21

X2J946

NP_001286075

AHN54589

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1488 aa isoforms: Nak-PA, Nak-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

1490 (aa)

(Chien et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nak using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nak_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.28

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Chien et al., 1998)

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

central brain anlage in statu nascendi

(Fisher et al., 2012)

mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

head mesoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

garland cell primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval garland cell

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Chien et al., 1998)

embryonic stage 1 -- 15

Comment: reference states 0-12 hr AEL

(Chien et al., 1998)

Additional Descriptive Data

The 5.5kb Nak transcript is detected at all stages of development.

(Chien et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Yang et al., 2011)

located_in dendrite

inferred from direct assay

(Yang et al., 2011)

located_in neuronal cell body

inferred from direct assay

(Yang et al., 2011)

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}Nak^LH-4

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

eye disc