Comment: 0-22 hr AEL
TCAGTTTCTGGCTCCGTTTAACAATAACTTGGCTGCCATGGGCGACGGTCAGCAGACTTTGGCTAGTGCTGCCTCGAATC CCGGGATATATGCATCTTCAACGGCAAACTCAGCCGCGGTATCCATTAGCGAATTAATGAAACCAGGACAAACGGCGGTG GAAAAGCGAGTTGAAGCCTGGAATCCGTTCGAAGAGGAGCAGCCTTTCAGCCAGATGACTGAGGATCACATCTTTGAGGC GGAGTTCGACAAGATACGACAGAGAGGCAGTCAAGGAAGCATCACAGCAAAATCGGCATCCACAACTTCCACGCTAACAC CAACGGAGCAGATTCCCTCTGCGGTTGTGGCTCCTGTTACAGCTGCTCCTGGTGCAGTGCCTCCTCCGCCGATTGCTGCC GCTGTGTCTCCTTACCCGTCGCAGGTCTCCGAGGATCCATTCGGATCGGCGCCATTCAGTTTGCCACCCGGACTGCGCGA GAAGGCGAGCTCGCTGCGCAAAACCGGAGCGAAATTCATCGGCGTTTCATCTGGTGGAACAGGAGGCGGAGTGGCCACGC CTATCGGCTTGGTAGCGGGAGGAGCAAACACCGCCACCTCTTCCAACTCATCCAAGTGGATGCTCTCCCCCACCCTGGAG GTTTCCAGCGAAGAGAAGACCTCGCTTATCAGTAAACTCAAAACAGACTCAGAATGTGGAATTCCAGGCGCTGTTGGAGG AGCTGGAGAAGGTGTCTCTGGTTATGTTAAGCTACCTGTAGAAGATCGCAACAAGTACGAGAAGCTGCGCAGCAAAGATC AGCCGACTTCCGATGACTCCGATTCCGAGGCTTCGGAGTTCTTTCAACAAGACAGCGATGAAGGAGGTGGGGGCATCTTT AGGCAAATGCAGATGGTGACAAGCAATTTGCCGGAAACCATCCATAAAATCCAACAGGTTGCCTATCACAAGGTGGACAA GACAGCTCATCTGCCAATTGTGAAGAAACTTCGCGATAAGACCAAGAAACCCACAACAGCTGCCCATCAAGCGGCCCAAC AACTAAATCTCATGGCTGCCGCGGTAGCTGCACAAGCTGAAGAAGCTGCCAAGGCGGCGGAATCCGACGGTGATTCGATC GGATCTGCCAGTGACCTGCGGGCAGAGGATGATGATCTGTTTGACGAGAATCCTCGCCAGACACAAGCCAAGCAACGTCG TCCTCTGCCAACAGAAATGGATGGAATAAGCGAGTCAGTGAAGACCTGTAGCAGCTCTGCCTACCATGCAGAATGTGAAA GTGTGACCACCCATGAAGACGATGTGCGTAGTCGGGTGGTGGTGAAGGTGCGGATGAGGAAGAAGGACCGTGCCCTTGTG ACTGCCACTGGCGACCCAAGTGCTCCGTCTGAAGAACTAAGTCCCACTTCGAGCGATCTCTTGCACAAGTTCGGCGATCG TCCGTTGCTGCTAGATGATGAATTGGATTATGGTTCTGGAGAAAGCAAGAGTAGCACTGAATCTCCTGTGGCTCCAATTC TGCCAACAAATCCCGATGTGCAGGAATCAGAGGAGGATGTGTTCGCCCTGGCTCCATTTAAATTACCAGTCGGAGTTCCT TTGAAACGAAAGACTAAACCCAAAGCTAAGCCCAAGCCTTCGGAACCCGAGATGTGGACTTCAACGCCAGTTAAAAGCGC CGCTGTCGGAGATCCTTCTACCAACTTTGCCAGTTTTCCAGCGCAGCTCACGCCAACCAATAATGTGGGGAACTCTGCGC AGCTGGATGAGTTCAATGAGCCAATTTTCAATCCCTTCCAGGAAAACGCACAGAACACGCAATCAGGGGAAACTAATCAA TGCGACCTGTTCGGCTTGGAACCATTTCCACAGGTAATTCGTACAATCGAACAGAACCCACAGCACCACCAGATCCACCA ATTTCCCAGCCACAACAACAATAACAATGTGATCAAAGTGCCCGCCTCCGTATCTGTTGCTCTTCCTGCCTCCGTTCCTG TATCTGTTTCCGTCAATTCGTCTGCCCCACAACTGGTGACCATAAATCACTCGATTATTATCAACAAAACGCCGGAGCCA CCGATGAACTATAATAATAATAATAATAACCAGACTGCTAAGTCGAGTGGCACCAGCAGTGTCTATGTGAATGTGCCGTC TTTCTCCAGCATGAGCACCAGTTCCTGCTCCATTAACCAGCCACAATCCTCACCAGCAGCTGTGATTCCAGTTCAGGCAC CTCTTCCAGTTCCAGTTCCTGCCCCTGTTTCCGTTCCAGCCGTTCCAGTTCCAATACCAGCGGTCGTTACCCATCTGCGT CCTTTGCTGCCCATTGCCGATAATGCGTATGTGCAAATCTCCCAAGATCGCTGCTCAGATTTCACACCGGACGACGAGGA GGAACCCGTGGTGTTGCGTGATGCCGATGTGGTGGCAGACGGAATCGGTATACCTACCCCTGCGAAACCCAAGAAGGAGA AGTCCAATCTGGCTGTAAGGGTTCTGCCGGGAAAGCTCACCCAAAAGGTAAAGGGGCATTCGTACAAAAAAGTAAGCGCG GCTGCCCTTGGATCCACCAGTTCCCTTAGTTCCGGCAGCAAGCAGAAGCACCAGCGATTGCAGTACCAGTCCCACGATGA TGACGACGACGAACAGGATCAGGATGAGAATCGAGGTGCTGGAGGCGGCCGCAATTTCCAATCAAACACCATTCAAAATT CTGCCAAAACCGGCTTCAGCAACATGAGCTTTGAGGACTTTCCCTCGGATCAGGAAATGGATCGTCTGTCCAGGACCATT CCCTTCGAGGTGGTTAGAAACGAAAAGATGTTACTCGAGGCGGAGAAGAAGTTCGGCTCCCTCAAGCGACGGAACAACTT GTTCTCATAATGTGGACAGTGGGGCGAGACCATCAAGGAGACCTGAGACTGAGTGTTTCAAATTATGTTGACATTTCGTT TAAGCTTTTCTCCGTTCCGTTTTTAATGTGGGGTTCCAAAAATAACCAATATCTGTATTTTTTGAATATAGTATTTATTC GCGTGTAGCAATGTTAAAAAGTGGCTAGTACCCATACATATTATACACACAACAAGAACAATACCTATAACTGTTATTTA GTGCAAAAATTTAAACTAGTTTTAGAAGCGAAACATGTTTTAGAATTTATACAGTGCGTAAGTTTACCAAAGTTACATTT ATTTTTGTGGCATTTTTCAGCTTGAAGTATTTCATGAATTATATATTTATACAAAACTATGTGATTATACACCCACACCC AATAACAACCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
