FlyBase Gene Report: Dmel\Sik3

General Information

Symbol

Dmel\Sik3

Species

D. melanogaster

Name

Salt-inducible kinase 3

Annotation Symbol

CG42856

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262103

Gene Model Status

Current

Stock Availability

16 publicly available

Enzyme Name (EC)

Gene Summary

cooperates with Mondo-Mlx to maintain organismal sugar tolerance through the regulation of NADPH/NADP+ redox balance - expression in clock neurons-morning oscillators (M cells) regulates male sex drive - feeding and fasting signals converge on the LKB1-SIK3 pathway to regulate lipid metabolism -regulates growth through the Hippo signalling pathway (Interactive Fly, Sik3 )

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

SIK

Key Links

Genomic Location

Cytogenetic map

55E6-55E9

Sequence location

2R:18,688,132..18,705,805 [+]

Recombination map

2-86

RefSeq locus

NT_033778 REGION:18688132..18705805

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein serine/threonine kinase activity

inferred from direct assay

(Teesalu et al., 2017, Wehr et al., 2013, Wang et al., 2011)

inferred from mutant phenotype

(Choi et al., 2015)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000678822

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in circadian sleep/wake cycle, sleep

inferred from mutant phenotype

(Funato et al., 2016)

involved_in insulin receptor signaling pathway

inferred from direct assay

(Wang et al., 2011)

inferred from genetic interaction with FLYBASE:InR; FB:FBgn0283499

(Choi et al., 2015)

involved_in lipid homeostasis

inferred from mutant phenotype

(Wang et al., 2011)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Wehr et al., 2013)

involved_in negative regulation of triglyceride catabolic process

inferred from mutant phenotype

(Choi et al., 2015, Wang et al., 2011)

involved_in positive regulation of pentose-phosphate shunt

inferred from mutant phenotype

(Teesalu et al., 2017)

involved_in response to starvation

inferred from mutant phenotype

(Wang et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN008613719

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001552066

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001552066

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES

Pathway (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -: The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).

Pathway (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS -: The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sik3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1ZBC4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sik3-RA

FBtr0304024

NM_137515

2983

702

Sik3-RB

FBtr0304025

NM_137517

5637

1471

Sik3-RC

FBtr0304026

NM_001202040

6469

1471

Sik3-RD

FBtr0340388

NM_001299658

6278

1468

Sik3-RE

FBtr0344258

NM_001103900

6020

1382

Sik3-RF

FBtr0344259

NM_001103899

6011

1379

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sik3-PA

FBpp0292993

77.4

702

7.32

FBpp0292994

158.2

1471

6.75

FBpp0292995

158.2

1471

6.75

FBpp0309334

157.9

1468

6.75

FBpp0310663

148.7

1382

6.52

FBpp0310664

148.4

1379

6.52

A8DYI4

NP_001097369

ABV53845

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1471 aa isoforms: Sik3-PB, Sik3-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sik3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sik3_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.02

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sik3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sik3

View exonic expression by tissue for Dmel\Sik3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262103

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37152/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262103

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262103

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18604

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 26 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sik3

Transgenic constructs containing regulatory region of Sik3

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel7157

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(2R)Sik3^Δ109

(Şahin et al., 2020)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Sik3^Δ109

lethal | recessive

lethal - all die before end of larval stage

Sik3⁷²

lethal - all die before end of larval stage (with Df(2R)P34)

Sik3⁷²

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Sik3^GD8535

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

Sik3^HMS01922

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Sik3^GL00292

fertile

Other Phenotypes

Allele

abnormal sleep

Sik3⁴⁸

abnormal sleep | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

Sik3^S563A.UAS

abnormal starvation stress response

Sik3⁴⁸

some die during larval stage

Sik3⁷²

some die during larval stage (with Df(2R)P34)

Sik3⁷²

Phenotype manifest in

Allele

eye, with Scer\GAL4^GMR.long, Scer\GAL4^ey.PH

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (94)

Hsap\SIK3

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Hsap\SIK1B

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (81)

Rnor\Sik3

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\RGD1562638

2 of 14

Yes

Rnor\RGD1565143

2 of 14

Yes

Rnor\Sik1

2 of 14

Rnor\Smok2a

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

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1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tssk6

1 of 14

Rnor\Ttk

1 of 14

Mus musculus (laboratory mouse) (98)

Mmus\Sik3

3 of 14

Yes

Mmus\1810024B03Rik

2 of 14

Yes

Mmus\4921509C19Rik

2 of 14

Yes

Mmus\Gm4567

2 of 14

Yes

Mmus\Gm4922

2 of 14

Yes

Mmus\Gm5157

2 of 14

Yes

Mmus\Gm5891

2 of 14

Yes

Mmus\Gm6176

2 of 14

Yes

Mmus\Gm6882

2 of 14

Yes

Mmus\Gm7168

2 of 14

Yes

Mmus\Gm10662

2 of 14

Yes

Mmus\Gm10668

2 of 14

Yes

Mmus\Gm14147

2 of 14

Yes

Mmus\Gm14151

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Mmus\Smok2b

2 of 14

Yes

Mmus\Smok3a

2 of 14

Mmus\Smok3b

2 of 14

Mmus\Smok3c

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm4565

1 of 14

Yes

Mmus\Gm5890

1 of 14

Yes

Mmus\Gm6902

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tssk6

1 of 14

Mmus\Ttk

1 of 14

Xenopus tropicalis (Western clawed frog) (46)

Xtro\aurka

1 of 13

Yes

Xtro\camk1

1 of 13

Yes

Xtro\camk2a

1 of 13

Yes

Xtro\camk2g

1 of 13

Yes

Xtro\chek2

1 of 13

Yes

Xtro\chpt1

1 of 13

Yes

Xtro\csnk1d

1 of 13

Yes

Xtro\dscaml1

1 of 13

Yes

Xtro\itm2a

1 of 13

Yes

Xtro\lats2

1 of 13

Yes

Xtro\LOC100145764

1 of 13

Yes

Xtro\LOC100485259

1 of 13

Yes

Xtro\LOC100486333

1 of 13

Yes

Xtro\LOC100494322

1 of 13

Yes

Xtro\LOC116406512

1 of 13

Yes

Xtro\map3k13

1 of 13

Yes

Xtro\mark1

1 of 13

Yes

Xtro\mark2

1 of 13

Yes

Xtro\mark4

1 of 13

Yes

Xtro\mastl

1 of 13

Yes

Xtro\melk

1 of 13

Yes

Xtro\nek1

1 of 13

Yes

Xtro\nek4

1 of 13

Yes

Xtro\nek5

1 of 13

Yes

Xtro\nuak1

1 of 13

Yes

Xtro\nuak2

1 of 13

Yes

Xtro\pdik1l

1 of 13

Yes

Xtro\pkn3

1 of 13

Yes

Xtro\prkaa1

1 of 13

Yes

Xtro\rock1

1 of 13

Yes

Xtro\sbk1l

1 of 13

Yes

Xtro\sbk2

1 of 13

Yes

Xtro\sgk3

1 of 13

Yes

Xtro\sik3

1 of 13

Yes

Xtro\snrk

1 of 13

Yes

Xtro\speg

1 of 13

Yes

Xtro\stk10

1 of 13

Yes

Xtro\stk16

1 of 13

Yes

Xtro\stk32c

1 of 13

Yes

Xtro\stk35

1 of 13

Yes

Xtro\taok1

1 of 13

Yes

Xtro\tmem18

1 of 13

Yes

Xtro\tmem52

1 of 13

Yes

Xtro\trib1

1 of 13

Yes

Xtro\ttk

1 of 13

Yes

Xtro\ulk1

1 of 13

Yes

Danio rerio (Zebrafish) (86)

Drer\mark2a

2 of 14

Yes

Drer\mark2b

2 of 14

Yes

Drer\mark3a

2 of 14

Yes

Drer\mark3b

2 of 14

Yes

Drer\sik1

2 of 14

Yes

Drer\sik2a

2 of 14

Yes

Drer\sik2b

2 of 14

Yes

Drer\sik3

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

Drer\si:ch211-63o20.7

1 of 14

Drer\si:ch211-171h4.3

1 of 14

Drer\si:dkey-8e10.3

1 of 14

Drer\si:dkey-81j8.6

1 of 14

Drer\si:dkey-192d15.2

1 of 14

Drer\si:zfos-2326c3.2

1 of 14

1 of 14

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1 of 14

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1 of 14

Caenorhabditis elegans (Nematode, roundworm) (60)

Cele\K02E10.7

2 of 14

Yes

Cele\kin-29

2 of 14

Yes

1 of 14

1 of 14

1 of 14

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1 of 14

Yes

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (35)

Agam\AgaP_AGAP006411

3 of 12

Yes

Agam\AgaP_AGAP012064

3 of 12

Yes

Agam\AgaP_AGAP013439

3 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (87)

Atha\ADK1

1 of 13

Yes

Atha\AT1G30640

1 of 13

Yes

Atha\AT1G48490

1 of 13

Yes

Atha\AT1G54610

1 of 13

Yes

Atha\AT1G71530

1 of 13

Yes

Atha\AT2G19400

1 of 13

Yes

Atha\AT2G20470

1 of 13

Yes

Atha\AT2G32850

1 of 13

Yes

Atha\AT2G36350

1 of 13

Yes

Atha\AT2G37840

1 of 13

Yes

Atha\AT3G01085

1 of 13

Yes

Atha\AT3G13670

1 of 13

Yes

Atha\AT3G17850

1 of 13

Yes

Atha\AT3G23310

1 of 13

Yes

Atha\AT3G53930

1 of 13

Yes

Atha\AT3G61960

1 of 13

Yes

Atha\AT4G14350

1 of 13

Yes

Atha\AT5G09890

1 of 13

Yes

Atha\AT5G18700

1 of 13

Yes

Atha\AT5G21222

1 of 13

Yes

Atha\CAK1AT

1 of 13

Yes

Atha\CIPK1

1 of 13

Yes

Atha\CIPK2

1 of 13

Yes

Atha\CIPK3

1 of 13

Yes

Atha\CIPK4

1 of 13

Yes

Atha\CIPK5

1 of 13

Yes

Atha\CIPK7

1 of 13

Yes

Atha\CIPK8

1 of 13

Yes

Atha\CIPK9

1 of 13

Yes

Atha\CIPK12

1 of 13

Yes

Atha\CIPK13

1 of 13

Yes

Atha\CIPK15

1 of 13

Yes

Atha\CIPK16

1 of 13

Yes

Atha\CIPK17

1 of 13

Yes

Atha\CIPK18

1 of 13

Yes

Atha\CIPK19

1 of 13

Yes

Atha\CIPK20

1 of 13

Yes

Atha\CIPK21

1 of 13

Yes

Atha\CIPK22

1 of 13

Yes

Atha\CIPK23

1 of 13

Yes

Atha\CIPK25

1 of 13

Yes

Atha\CIPK26

1 of 13

Yes

Atha\CKL2

1 of 13

Yes

Atha\ckl3

1 of 13

Yes

Atha\ckl4

1 of 13

Yes

Atha\ckl5

1 of 13

Yes

Atha\CKL6

1 of 13

Yes

Atha\ckl7

1 of 13

Yes

Atha\ckl8

1 of 13

Yes

Atha\ckl10

1 of 13

Yes

Atha\ckl12

1 of 13

Yes

Atha\CKL13

1 of 13

Yes

Atha\CPK6

1 of 13

Yes

Atha\CPK18

1 of 13

Yes

Atha\CPK26

1 of 13

Yes

Atha\GRIK1

1 of 13

Yes

Atha\IRE

1 of 13

Yes

Atha\KIN10

1 of 13

Yes

Atha\KIN11

1 of 13

Yes

Atha\MAP3KA

1 of 13

Yes

Atha\MKK2

1 of 13

Yes

Atha\MPK4

1 of 13

Yes

Atha\MPK5

1 of 13

Yes

Atha\MPK6

1 of 13

Yes

Atha\NEK3

1 of 13

Yes

Atha\NEK4

1 of 13

Yes

Atha\NP1

1 of 13

Yes

Atha\OST1

1 of 13

Yes

Atha\PEPKR1

1 of 13

Yes

Atha\PK3

1 of 13

Yes

Atha\PPCK2

1 of 13

Yes

Atha\SIK1

1 of 13

Yes

Atha\SIP1

1 of 13

Yes

Atha\SIP3

1 of 13

Yes

Atha\SIP4

1 of 13

Yes

Atha\SnRK1.3

1 of 13

Yes

Atha\SNRK2-8

1 of 13

Yes

Atha\SNRK2.1

1 of 13

Yes

Atha\SNRK2.2

1 of 13

Yes

Atha\SNRK2.3

1 of 13

Yes

Atha\SNRK2.4

1 of 13

Yes

Atha\SNRK2.5

1 of 13

Yes

Atha\SNRK2.7

1 of 13

Yes

Atha\SNRK2.10

1 of 13

Yes

Atha\SOS2

1 of 13

Yes

Atha\SR1

1 of 13

Yes

Atha\YDA

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (34)

Scer\FRK1

2 of 13

Yes

Scer\KIN4

2 of 13

Yes

Scer\PRR1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (27)

Spom\ppk1

2 of 12

Yes

Spom\ppk27

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Spom\ppk24

1 of 12

Spom\ppk34

1 of 12

Spom\ran1

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sik3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (41)

Sik2

4 of 13

AMPKα

2 of 13

CG4629

2 of 13

grp

2 of 13

KP78a

2 of 13

KP78b

2 of 13

par-1

2 of 13

sff

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

aux

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

dco

1 of 13

hpo

1 of 13

meng

1 of 13

Myt1

1 of 13

Nak

1 of 13

Nek2

1 of 13

niki

1 of 13

Nuak

1 of 13

Pak

1 of 13

Pdk1

1 of 13

S6kII

1 of 13

Slik

1 of 13

sti

1 of 13

trbl

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Sik3^HMS01922

ameliorates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Sik3^S563A.PA.UAS

exacerbates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Sik3^UAS.Tag:MYC

exacerbates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SIK3; SIK family kinase 3

3 of 14

614776

SPONDYLOEPIMETAPHYSEAL DYSPLASIA, KRAKOW TYPE; SEMDK

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Sik3 - G6pd

protein kinase assay, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Teesalu et al., 2017)

Sik3 - Hipk

anti tag coimmunoprecipitation, western blot

(Yu et al., 2023)

Sik3 - sav

anti tag coimmunoprecipitation, anti tag western blot

(Wehr et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

fng

enhanceable

Sik3

(Şahin et al., 2020)

Ser

enhanceable

Sik3

(Şahin et al., 2020)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262103

MIST (protein-protein) - An integrated Molecular Interaction Database

37152

Pathways

Signaling Pathways (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS
NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q4QQA7

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-86

Cytogenetic map

55E6-55E9

Sequence location

2R:18,688,132..18,705,805 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55E6-55E9

Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)03709⁰³⁷⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-91.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18634

w¹¹¹⁸; PBac{WH}Sik3^f03280

28366

y¹ v¹; P{TRiP.JF03002}attP2

15962

y¹ w^67c23; P{EPgy2}Sik3^EY06260

20921

y¹ w^67c23; P{EPgy2}Sik3^EY14354

59750

y¹ w^*; Mi{MIC}Sik3^MI15336/SM6a

66380

y¹ w^*; Mi{PT-GFSTF.0}Sik3^{MI15336-GFSTF.0}/CyO

35378

y¹ sc^* v¹ sev²¹; P{TRiP.GL00292}attP2

NIG-Fly

15072R-1

P{NIG.15072R}1

GL00292

y¹ sc^* v¹ sev²¹; P{TRiP.GL00292}attP2

VDRC

v106268

P{KK104229}VIE-260B

v107458

P{KK109965}VIE-260B

v39866

w¹¹¹⁸; P{GD8535}v39866

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (27)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262103

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0262103

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Wang et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, spindle abnormalities, chromosome alignment defects are seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG15072 CG18604

(FlyBase Genome Annotators, 2010)

Additional comments

Annotations CG15072 and CG18604 merged as CG42856 in release 5.30 of the genome annotation.

(FlyBase Genome Annotators, 2010)

Nomenclature History

Source for database identify of

Source for identity of: Sik3 CG42856

(Wang et al., 2011)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG15072

(Couderc et al., 2017, Ugrankar et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2011.7.19, Wang et al., 2011, Amin et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Liebl and Featherstone, 2005)

CG18604

(Keleman et al., 2009.8.5, Rehwinkel et al., 2006)

CG42856

(Wehr et al., 2013, Friedman et al., 2011, FlyBase Genome Annotators, 2010, Ni et al., 2010.12.1)

EMK/KIAA0999

(Morrison et al., 2000)

SIK

(Lam Wong and Verheyen, 2021, Amin et al., 2009)

SIK3

(Lones and DiAntonio, 2023, Chatterjee and Perrimon, 2021, Funato, 2020, Toprak et al., 2020, Havula and Hietakangas, 2018, Lehmann, 2018, Musselman and Kühnlein, 2018, Couderc et al., 2017, Mattila and Hietakangas, 2017, Teesalu et al., 2017, Choi et al., 2015, Wang et al., 2011)

Name Synonyms

Salt-inducible kinase 3

(Havula and Hietakangas, 2018, Ly et al., 2018, Mattila and Hietakangas, 2017, Teesalu et al., 2017)

Secondary FlyBase IDs

FBgn0034376
FBgn0034378

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 64 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37152

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1ZBC4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1ZBC4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262103

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37152/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262103

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262103

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42856

MARRVEL_MODEL - MARRVEL (model organism gene)

37152

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262103

DroID - A comprehensive database of gene and protein interactions.

FBgn0262103

DRSC - Results frm RNAi screens

FBgn0262103

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sik3_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0262103

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18604

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015081

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

37152

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q4QQA7

References (91)

The following external sites may use different assemblies or annotations than FlyBase.

-1.02

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