projectin, twitchin, l(4)23, l(4)2, CT8086
Gene model reviewed during 5.55
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.47
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bt using the Feature Mapper tool.
Indirect evidence suggests that the bt transcript is only expressed in larval and adult muscle.
bt protein can be detected in scattered aggregates dispersed in the cytoplasm of muscle cell precursors as early at 30 hrs APF. By 48 hours bt, along with other myofibrillar proteins, have organized into Z-bands.
bt protein was immunolocalized to indirect flight muscle with a Lethocerus antibody. It localizes to flight muscles in the region containing C-filaments immediately adjacent to Z-discs.
bt protein is first detected by westerns blots at the mid-embryo stage and increases progressively until the end of the adult stage. The location of bt protein differs in nonfibrillar muscle from that in flight muscles. In the latter, it is located around the Z disc while in tubular muscle, it is located on the A band with a gap in the middle.
GBrowse - Visual display of RNA-Seq signalsView Dmel\bt in GBrowse 2
Mapped in diplo-4 triploids.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: bt CG1479
Source for merge of: bt CG10285
Annotations CG10285, CG1479 merged as CG32019 in release 3 of the genome annotation.
Hochman isolated 3 spontaneous alleles, 6 X ray induced alleles, 23 ethyl methanesulfonate induced alleles and 3 ICR170 induced alleles.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
"Identified with: GM13171" was stated as revision. "Identified with: GM10074" was stated as revision. "Identified with: HL02324" was stated as revision. "Identified with: HL05966" was stated as revision.
EST HL02324, referred to as "myosin LCK" in FBrf123240, actually corresponds to bt. EST GM13171, referred to as "titin" in FBrf123240, actually corresponds to bt. EST GM10074, referred to as "C-protein" in FBrf123240, actually corresponds to bt.
Isolation and partial characterization of bt, includes identification of twitchin-like motifs.
An analysis of bt gene product, projectin, was performed to present evidence that projectin and twitchin may share functional protein kinase domains. As described for titin, bt is also demonstrated to be phosphorylated in vivo at Ser residues.
bt is a single copy gene containing a two motif amino acid sequence pattern that characterizes twitchin and titin. bt appears to encode projectin: a muscle protein thought to play a structural role in asynchronous flight muscle.
A protein component of connecting filaments of both asynchronous and synchronous muscles. In synchronous muscles projectin is found in the region of the thick filaments; in asynchronous muscles it is between the Z-band and thick filaments. The projectin of the two classes of muscle are similar, if not identical, by SDS electrophoresis and peptide mapping.