FlyBase Gene Report: Dmel\sqa

General Information

Symbol

Dmel\sqa

Species

D. melanogaster

Name

spaghetti-squash activator

Annotation Symbol

CG42347

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259678

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

[myosin light-chain] kinase (2.7.11.18)

Gene Summary

spaghetti-squash activator (sqa) encodes a myosin light chain kinase-like protein. It is a substrate of the kinase encoded by Atg1 and is required for starvation-induced autophagy. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

46B1-46B2

Sequence location

2R:9,716,348..9,748,121 [-]

Recombination map

2-61

RefSeq locus

NT_033778 REGION:9716348..9748121

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein kinase activity

inferred from genetic interaction with FLYBASE:Tsc1; FB:FBgn0290397

(Findlay et al., 2007)

enables protein serine/threonine kinase activity

inferred from mutant phenotype

(Tang et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables myosin light chain kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN002389378

(GO Reference Genome Project, 2011-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in autophagy

inferred from mutant phenotype

(Tang et al., 2011)

involved_in compartment boundary maintenance

inferred from mutant phenotype

(Ku and Sun, 2017)

involved_in eye-antennal disc morphogenesis

inferred from mutant phenotype

(Ku and Sun, 2017)

involved_in morphogenesis of an epithelial fold

inferred from mutant phenotype

(Ku and Sun, 2017)

involved_in positive regulation of TOR signaling

inferred from genetic interaction with FLYBASE:Tsc1; FB:FBgn0290397

(Findlay et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000757516

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000686485

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

myosin light chain kinase activity

(1) L-seryl-[myosin light chain] + ATP = O-phospho-L-seryl-[myosin light chain] + ADP + H(+) (2.7.11.18)

(2) L-threonyl-[myosin light chain] + ATP = O-phospho-L-threonyl-[myosin light chain] + ADP + H(+) (2.7.11.18)

RHEA 22004: protein serine/threonine kinase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -: The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\sqa for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z7Y7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.42

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

sqa-RB

FBtr0299929

NM_136670

2057

446

sqa-RC

FBtr0299930

NM_165693

3214

888

sqa-RD

FBtr0310326

NM_001273903

6062

888

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

sqa-PB

FBpp0289206

50.3

446

4.82

FBpp0289207

97.6

888

5.43

FBpp0302009

97.6

888

5.43

A1Z7Y7

NP_001260832

AGB93365

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

888 aa isoforms: sqa-PC, sqa-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sqa using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sqa_1
sqa_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.78

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\sqa in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\sqa

View exonic expression by tissue for Dmel\sqa

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259678

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36002/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259678

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259678

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259678

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1776

Flygut - An atlas of the Drosophila adult midgut

FBgn0259678

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of sqa

Transgenic constructs containing regulatory region of sqa

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel9016

(Ryder, 2004.4.26)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

sqa^GD15058

viable, with Scer\GAL4^Mef2.PR

sqa^GD15058

viable, with Scer\GAL4^pnr-MD237

sqa^GD15058

viable, with Scer\GAL4^tin.CΔ4

sqa^GD15058

Other Phenotypes

Allele

short lived, with Scer\GAL4^Cg.PA

sqa^T279A.UAS

short lived | calorie restriction conditional, with Scer\GAL4^Cg.PA

sqa^RNAi.UAS

visible, with Scer\GAL4^pnr-MD237

sqa^GD15058

visible, with Scer\GAL4^ptc-559.1

sqa^UAS.cTa

visible | somatic clone

sqa^f01512

Phenotype manifest in

Allele

adult head | somatic clone

sqa^f01512

anterior crossvein, with Scer\GAL4^ptc-559.1

sqa^UAS.cTa

autophagosome | somatic clone | calorie restriction conditional, with Scer\GAL4^Act5C.PP

eye | adult stage | somatic clone

sqa^f01512

eye-antennal disc | larval stage | somatic clone

sqa^f01512

fat body | somatic clone | calorie restriction conditional, with Scer\GAL4^Act5C.PP

mesonotum, with Scer\GAL4^pnr-MD237

sqa^GD15058

ommatidium | adult stage | somatic clone

sqa^f01512

trichogen cell, with Scer\GAL4^pnr-MD237

sqa^GD15058

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (44)

Hsap\MYLK2

5 of 14

Yes

Hsap\MYLK3

5 of 14

Yes

Hsap\MYLK4

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\DAPK3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ULK2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (29)

Rnor\Mylk2

6 of 14

Yes

Rnor\Mylk3

5 of 14

Yes

Rnor\Mylk4

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Uhmk1

1 of 14

Yes

Mus musculus (laboratory mouse) (34)

Mmus\Mylk2

6 of 14

Yes

Mmus\Mylk3

6 of 14

Yes

Mmus\Mylk4

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (28)

Xtro\mylk3

3 of 13

Yes

Xtro\mylkl

3 of 13

Yes

Xtro\mylk4

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (48)

Drer\mylk3

5 of 14

Yes

Drer\mylk2

4 of 14

Yes

Drer\mylk4b

4 of 14

Yes

Drer\mylk5

4 of 14

Yes

Drer\mylk4a

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\dapk2a

2 of 14

Yes

Drer\dapk3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Drer\si:dkey-240h12.4

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (15)

Cele\mlck-1

5 of 14

Yes

Cele\cmk-1

2 of 14

Cele\dapk-1

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\zyg-8

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP006746

5 of 12

Yes

Agam\AgaP_AGAP010632

4 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (50)

Atha\CDPK1

2 of 13

Yes

Atha\CDPK6

2 of 13

Yes

Atha\CDPK9

2 of 13

Yes

Atha\CDPK19

2 of 13

Yes

Atha\CPK1

2 of 13

Yes

Atha\CPK2

2 of 13

Yes

Atha\CPK5

2 of 13

Yes

Atha\CPK6

2 of 13

Yes

Atha\CPK7

2 of 13

Yes

Atha\CPK9

2 of 13

Yes

Atha\CPK14

2 of 13

Yes

Atha\CPK15

2 of 13

Yes

Atha\CPK17

2 of 13

Yes

Atha\CPK18

2 of 13

Yes

Atha\CPK19

2 of 13

Yes

Atha\CPK20

2 of 13

Yes

Atha\CPK21

2 of 13

Yes

Atha\CPK22

2 of 13

Yes

Atha\CPK24

2 of 13

Yes

Atha\CPK25

2 of 13

Yes

Atha\CPK26

2 of 13

Yes

Atha\CPK27

2 of 13

Yes

Atha\CPK28

2 of 13

Yes

Atha\CPK29

2 of 13

Yes

Atha\CPK30

2 of 13

Yes

Atha\CPK31

2 of 13

Yes

Atha\CPK32

2 of 13

Yes

Atha\CPK33

2 of 13

Yes

Atha\CPK34

2 of 13

Yes

Atha\PEPKR1

2 of 13

Yes

Atha\PEPKR2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\CMK1

2 of 13

Yes

Scer\CMK2

2 of 13

Yes

Scer\MEK1

2 of 13

Yes

Scer\RCK2

2 of 13

Yes

Scer\TDA1

2 of 13

Yes

Scer\IKS1

1 of 13

Yes

Scer\NNK1

1 of 13

Yes

Scer\PKH1

1 of 13

Scer\PKH2

1 of 13

Scer\RCK1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\cmk1

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:sqa. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

CaMKI

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Akt

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

PKD

1 of 13

sff

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MYLK2; myosin light chain kinase 2

model of hypertrophic cardiomyopathy 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYLK2; myosin light chain kinase 2

5 of 14

606566

CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 1; CMH1

hypertrophic cardiomyopathy 1

MYLK3; myosin light chain kinase 3

5 of 14

612147

MYLK4; myosin light chain kinase family member 4

4 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

sqa - Atg1

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

sqa

suppressible

sqh

(Tang et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Atg1

suppressible

sqa

(Tang et al., 2011)

Atg12

suppressible

sqa

(Tang et al., 2011)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0259678

MIST (protein-protein) - An integrated Molecular Interaction Database

36002

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A1Z7Y7

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-61

Cytogenetic map

46B1-46B2

Sequence location

2R:9,716,348..9,748,121 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

46B1-46B2

Limits computationally determined from genome sequence between P{lacW}l(2)k09221^k09221&P{PZ}dap⁰⁴⁴⁵⁴ and P{lacW}l(2)k05420^k05420

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-61

(FlyBase, 2016)

2-59.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26735

y¹ v¹; P{TRiP.JF02277}attP2

18446

w¹¹¹⁸; PBac{WH}sqa^f01512/CyO

67752

y¹ v¹; P{TRiP.HMC06304}attP2

63618

y¹ v¹; P{TRiP.HMJ30185}attP40

56428

y¹ w^*; Mi{MIC}sqa^MI11503

23043

y¹ w^67c23; Mi{ET1}sqa^MB01276

53393

y¹ w^*; Mi{MIC}sqa^MI09450/SM6a

NIG-Fly

30004R-1

P{NIG.30004R}1

HMJ30185

y¹ v¹; P{TRiP.HMJ30185}attP40/CyO

VDRC

v101640

P{KK105301}VIE-260B

v107241

P{KK105144}VIE-260B

v342732

P{hsFLP}1, y1 w1118; P{HD_CFD01455}attP40/CyO-GFP

More stocks available...

Genomic Clones (27)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (14)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0259678

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259678

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. Alterations of cell shape are also evident in S2R+ cells.

(Kiger et al., 2003)

New annotation (CG30004) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG1776 CG30004

(FlyBase Genome Annotators, 2008)

Additional comments

Annotations CG1776 and CG30004 merged as CG42347 in release 5.9 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Nomenclature History

Source for database identify of

Source for identity of: sqa CG42347

(Tang et al., 2011)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Name Synonyms

CG1776

CG30004

MLCK-like

(Morrison et al., 2000)

spaghetti-squash activator

(Tang et al., 2011)

Secondary FlyBase IDs

FBgn0033441
FBgn0050004

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36002

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z7Y7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z7Y7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259678

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36002/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259678

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259678

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42347

MARRVEL_MODEL - MARRVEL (model organism gene)

36002

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0259678

DroID - A comprehensive database of gene and protein interactions.

FBgn0259678

DRSC - Results frm RNAi screens

FBgn0259678

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sqa_1
sqa_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259678

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259678

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1776

Flygut - An atlas of the Drosophila adult midgut

FBgn0259678

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000228

MIST (protein-protein) - An integrated Molecular Interaction Database

36002

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A1Z7Y7

References (65)

The following external sites may use different assemblies or annotations than FlyBase.

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