FlyBase Gene Report: Dmel\IKKε

General Information

Symbol

Dmel\IKKε

Species

D. melanogaster

Name

I-kappaB kinase ε

Annotation Symbol

CG2615

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086657

Gene Model Status

Current

Stock Availability

21 publicly available

Enzyme Name (EC)

Gene Summary

I-kappaB kinase ε (IKKε) encodes a serine-threonine kinase that targets the inhibitor of apoptosis encoded by Diap1 for degradation. It also negatively regulates protein kinase C and the dynein adaptor encoded nuf. The product of IKKε contributes to the specification of bristle cells, bristle cell morphogenesis, oocyte polarity, and dendrite development. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

ik2, DmIKKε, l(2)38Ea, 38D.31, IκB kinase-like 2

Key Links

Genomic Location

Cytogenetic map

38D4-38D5

Sequence location

2L:20,676,045..20,678,979 [+]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:20676045..20678979

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (23 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9V9Y9

(Otani et al., 2015, Dubin-Bar et al., 2008)

enables protein serine/threonine kinase activity

inferred from mutant phenotype

(Kuranaga et al., 2006)

inferred from direct assay

(Otani et al., 2011)

enables small GTPase binding

inferred from physical interaction with FLYBASE:Rab30; FB:FBgn0031882

(Gillingham et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN001491808

(GO Reference Genome Project, 2011-)

Biological Process (16 terms)

Terms Based on Experimental Evidence (14 terms)

CV Term

Evidence

References

involved_in actin filament bundle organization

inferred from mutant phenotype

(Otani et al., 2011)

involved_in actin filament organization

inferred from mutant phenotype

(Oshima et al., 2006)

involved_in antiviral innate immune response

inferred from mutant phenotype

(Segrist et al., 2021)

involved_in cell morphogenesis

inferred from mutant phenotype

(Oshima et al., 2006)

involved_in cGAS/STING signaling pathway

inferred from mutant phenotype

(Segrist et al., 2021)

involved_in chaeta morphogenesis

inferred from mutant phenotype

(Bitan et al., 2010)

involved_in endocytic recycling

inferred from mutant phenotype

(Otani et al., 2011)

involved_in maintenance of epithelial integrity, open tracheal system

inferred from mutant phenotype

(Oshima et al., 2006)

involved_in microtubule bundle formation

inferred from mutant phenotype

(Bitan et al., 2010)

involved_in neuron remodeling

inferred from mutant phenotype

(Lee et al., 2009)

involved_in oocyte anterior/posterior axis specification

inferred from mutant phenotype

(Amsalem et al., 2013)

involved_in positive regulation of apoptotic process

inferred from mutant phenotype

(Kuranaga et al., 2006)

involved_in regulation of cytoskeleton organization

inferred from physical interaction with FLYBASE:spn-F; FB:FBgn0086362

(Dubin-Bar et al., 2008)

involved_in sensory organ precursor cell division

inferred from genetic interaction with FLYBASE:Diap1; FB:FBgn0260635

(Koto et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in activation of innate immune response

inferred from biological aspect of ancestor with PANTHER:PTN000540338

(GO Reference Genome Project, 2011-)

involved_in positive regulation of type I interferon production

inferred from biological aspect of ancestor with PANTHER:PTN000540338

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cell tip

inferred from direct assay

(Otani et al., 2011)

located_in cytoplasm

inferred from direct assay

(Otani et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001491808

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES

Pathway (FlyBase)

CGAS-STING SIGNALING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -: This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

CGAS-STING SIGNALING PATHWAY CORE COMPONENTS -: The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway is an anti-viral pattern recognition pathway. In Drosophila, cyclic GMP-AMP (cGAMP) synthases are activated by viral infection, cGAMP is in turn detected by STING ultimately resulting in the activation the NF-κB-like factor Rel and its translocation into the nucleus to activate the transcription of anti-viral genes. (Adapted from FBrf0252868.)

Summary (Interactive Fly)

regulation of nonapoptotic caspase activity, cytoskeleton, egg DV and AP polarity

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\IKKε for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3Y8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

IKKε-RB

FBtr0081390

NM_165337

2461

720

IKKε-RC

FBtr0336824

NM_136204

2551

720

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

IKKε-PB

FBpp0080920

82.0

720

6.88

FBpp0307790

82.0

720

6.88

Q9V3Y8

NP_610048

AAF53911

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

720 aa isoforms: IKKε-PB, IKKε-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\IKKε using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ik2_1
ik2_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.11

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell tip

inferred from direct assay

(Otani et al., 2011)

located_in cytoplasm

inferred from direct assay

(Otani et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\IKKε in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\IKKε

View exonic expression by tissue for Dmel\IKKε

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086657

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35329/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086657

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086657

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086657

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2615

Flygut - An atlas of the Drosophila adult midgut

FBgn0086657

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of IKKε

Transgenic constructs containing regulatory region of IKKε

Aberrations (Deficiencies and Duplications) ( 15 )

Inferred from experimentation ( 15 )

Gene not disrupted in

Df(2L)DS6

(Butler et al., 2001, Kozlova et al., 1998)

Df(2L)pr2b

(Butler et al., 2001)

Df(2L)pr37

(Butler et al., 2001)

Df(2L)pr8311

(Butler et al., 2001)

Df(2L)pr-A16

(Butler et al., 2001)

Df(2L)Sd14

(Butler et al., 2001)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(2L)ED1317)

lethal | recessive

lethal - all die before end of first instar larval stage | abnormal dorsal/ventral axis specification | maternal effect | recessive

lethal - all die before end of first instar larval stage | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

IKKε^GD5424

viable, with Scer\GAL4^Mef2.PR

IKKε^GD5424

viable, with Scer\GAL4^pnr-MD237

IKKε^GD5424

viable, with Scer\GAL4^tin.CΔ4

IKKε^GD5424

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

IKKε^GD5424

abnormal immune response | adult stage, with Scer\GAL4^NP0001

IKKε^HMS01188

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

IKKε^KK102408

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PG

IKKε^UAS.cKa

abnormal neuroanatomy | larval stage | heat sensitive, with Scer\GAL4^ppk.PG, Scer\GAL80^ts.αTub84B

IKKε^UAS.cKa

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

abnormal neuroanatomy | pupal stage | somatic clone

decreased occurrence of cell division | embryonic stage 13, with Scer\GAL4^sim.PS

IKKε^DN.UAS

increased cell death, with Scer\GAL4^GMR.PF

IKKε^UAS.cKa

increased cell death | larval stage, with Scer\GAL4^ppk.PG

IKKε^UAS.cKa

increased cell death | larval stage | heat sensitive, with Scer\GAL4^ppk.PG, Scer\GAL80^ts.αTub84B

IKKε^UAS.cKa

some die during first instar larval stage | maternal effect | recessive

some die during first instar larval stage | recessive

visible, with Scer\GAL4^neur-P72

visible, with Scer\GAL4^pnr-MD237

IKKε^GD5424

visible | adult stage

IKKε¹

visible | adult stage, with Scer\GAL4^sca.PU

Phenotype manifest in

Allele

actin filament bundle, with Scer\GAL4^ap-md544

arista lateral, with Scer\GAL4^ap-md544

IKKε^DN.UAS

border follicle cell, with Scer\GAL4^slbo.2.6

IKKε^UAS.cOa

chaeta, with Scer\GAL4^ap-md544

chaeta, with Scer\GAL4^neur-P72

cuticle | germline clone

dendrite, with Scer\GAL4^ppk.1.9

IKKε^KK102408

dendrite & dorsal multidendritic neuron ddaC | larval stage | conditional - heat sensitive, with Scer\GAL4^ppk.PG, Scer\GAL80^ts.αTub84B

IKKε^UAS.cKa

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

dendrite & dorsal multidendritic neuron ddaC | pupal stage | somatic clone

dendrite | larval stage, with Scer\GAL4^ppk.PG

IKKε^UAS.cKa

dendrite | pupal stage, with Scer\GAL4^ppk.PG

dorsal appendage | germline clone

egg chamber & microtubule | germ-line clone

embryo | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

IKKε^GL00160

embryonic/first instar larval cuticle | germline clone

embryonic abdomen | ectopic | germline clone

embryonic head | germline clone

embryonic tracheole, with Scer\GAL4^btl.PS

IKKε^GS11377

eye, with Scer\GAL4^GMR.PF

IKKε^UAS.cKa

humeral bristle

interommatidial bristle

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

IKKε^KK102408

macrochaeta, with Scer\GAL4^ap-md544

macrochaeta, with Scer\GAL4^{neur-GAL4-A101}

macrochaeta, with Scer\GAL4^sca-P309

IKKε^RNAi.UAS

macrochaeta | increased number, with Scer\GAL4^sca-P309

IKKε^UAS.cKa

mesothoracic bristle

IKKε¹

mesothoracic bristle, with Scer\GAL4^sca.PU

microtubule, with Scer\GAL4^{neur-GAL4-A101}

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

rhabdomere R7, with Scer\GAL4^Rh3.PP

IKKε^GS11377

scutellar bristle, with Scer\GAL4^{neur-GAL4-A101}

scutellar bristle, with Scer\GAL4^sca.PU

IKKε^DN.UAS

sensory mother cell | increased number, with Scer\GAL4^sca-P309

IKKε^UAS.cKa

terminal tracheal cell

IKKε⁵

terminal tracheal cell, with Scer\GAL4^btl.PS

IKKε^RNAi.UAS

terminal tracheal cell & filamentous actin

IKKε⁵

terminal tracheal cell & filopodium

IKKε⁵

trichogen cell, with Scer\GAL4^pnr-MD237

IKKε^GD5424

ventral midline of embryo | embryonic stage 13, with Scer\GAL4^sim.PS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (74)

Hsap\TBK1

13 of 14

Yes

Hsap\IKBKE

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\SIK1B

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (46)

Rnor\Tbk1

13 of 14

Yes

Rnor\Ikbke

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (61)

Mmus\Tbk1

13 of 14

Yes

Mmus\Ikbke

11 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm4565

1 of 14

Yes

Mmus\Gm5157

1 of 14

Mmus\Gm5890

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (31)

Xtro\tbk1

10 of 13

Yes

Xtro\ikbke

6 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\XB956263

1 of 13

Danio rerio (Zebrafish) (58)

Drer\ikbke

12 of 14

Yes

Drer\tbk1

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\mak

1 of 14

Drer\map3k1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (28)

Cele\Y39G8B.5

4 of 14

Yes

Cele\ikke-1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (19)

Agam\AgaP_AGAP009565

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (50)

Atha\AT1G49180

1 of 13

Yes

Atha\AT2G19230

1 of 13

Yes

Atha\AT2G28960

1 of 13

Yes

Atha\AT2G37840

1 of 13

Yes

Atha\AT2G41910

1 of 13

Yes

Atha\AT3G46140

1 of 13

Yes

Atha\AT3G53930

1 of 13

Yes

Atha\AT4G31170

1 of 13

Yes

Atha\AT5G18700

1 of 13

Yes

Atha\AT5G47070

1 of 13

Yes

Atha\AT5G50180

1 of 13

Yes

Atha\AUR1

1 of 13

Yes

Atha\CIPK8

1 of 13

Yes

Atha\CIPK12

1 of 13

Yes

Atha\CIPK21

1 of 13

Yes

Atha\CPK1

1 of 13

Yes

Atha\CPK2

1 of 13

Yes

Atha\CPK19

1 of 13

Yes

Atha\CPK20

1 of 13

Yes

Atha\CPK21

1 of 13

Yes

Atha\CPK22

1 of 13

Yes

Atha\CPK28

1 of 13

Yes

Atha\FU

1 of 13

Yes

Atha\KIN10

1 of 13

Yes

Atha\KIN11

1 of 13

Yes

Atha\MAP3KA

1 of 13

Yes

Atha\MAPKKK6

1 of 13

Yes

Atha\MAPKKK7

1 of 13

Yes

Atha\MAPKKK14

1 of 13

Yes

Atha\MAPKKK15

1 of 13

Yes

Atha\MEK1

1 of 13

Yes

Atha\MEKK1

1 of 13

Yes

Atha\MKK2

1 of 13

Yes

Atha\MKK3

1 of 13

Yes

Atha\MKK4

1 of 13

Yes

Atha\MKK7

1 of 13

Yes

Atha\MKK8

1 of 13

Yes

Atha\MKK9

1 of 13

Yes

Atha\MKK10

1 of 13

Yes

Atha\MPK12

1 of 13

Yes

Atha\NEK1

1 of 13

Yes

Atha\PEPKR1

1 of 13

Yes

Atha\PPK1

1 of 13

Yes

Atha\SIP3

1 of 13

Yes

Atha\SIP4

1 of 13

Yes

Atha\SNRK2.3

1 of 13

Yes

Atha\SNRK2.5

1 of 13

Yes

Atha\SNRK2.7

1 of 13

Yes

Atha\SNRK2.9

1 of 13

Yes

Atha\TMK1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (14)

Scer\ATG1

1 of 13

Yes

Scer\BCK1

1 of 13

Yes

Scer\CDC15

1 of 13

Yes

Scer\CMK1

1 of 13

Yes

Scer\KIC1

1 of 13

Yes

Scer\KIN1

1 of 13

Yes

Scer\MCK1

1 of 13

Yes

Scer\SNF1

1 of 13

Yes

Scer\SPS1

1 of 13

Yes

Scer\SSK22

1 of 13

Yes

Scer\STE11

1 of 13

Yes

Scer\STE20

1 of 13

Yes

Scer\TOS3

1 of 13

Yes

Scer\TPK1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\cdr1

1 of 12

Yes

Spom\kin1

1 of 12

Yes

Spom\mek1

1 of 12

Yes

Spom\mkh1

1 of 12

Yes

Spom\nak1

1 of 12

Yes

Spom\pck2

1 of 12

Yes

Spom\pka1

1 of 12

Yes

Spom\win1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:IKKε. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (23)

IKKβ

4 of 13

Aduk

1 of 13

Akt

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

hpo

1 of 13

KP78b

1 of 13

mbt

1 of 13

Mekk1

1 of 13

Nak

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

PKD

1 of 13

S6kII

1 of 13

sqa

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TBK1; TANK binding kinase 1

model of frontotemporal dementia and/or amyotrophic lateral sclerosis 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

IKKε^alice

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^Intron5.UAS

(Lu et al., 2020)

IKKε^GL00160

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Abreha et al., 2021)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^Intron5.UAS

(Lu et al., 2020)

IKKε^{UAS.ORF.Tag:HA}

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Abreha et al., 2021)

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Abreha et al., 2021)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^Intron5.UAS

(Lu et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TBK1; TANK binding kinase 1

13 of 14

TANK-BINDING KINASE 1; TBK1

frontotemporal dementia and/or amyotrophic lateral sclerosis 4

IKBKE; inhibitor of nuclear factor kappa B kinase subunit epsilon

12 of 14

605048

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

IKKε

anti tag coimmunoprecipitation, anti tag western blot

(Otani et al., 2011)

IKKε - Pkc53E

anti tag coimmunoprecipitation, anti tag western blot

(Otani et al., 2016)

IKKε - Rab11

anti tag coimmunoprecipitation, anti tag western blot

(Lin et al., 2020)

IKKε - Rab30

pull down, peptide massfingerprinting, western blot

(Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014, Sinka et al., 2008)

IKKε - ctp

anti tag coimmunoprecipitation, anti tag western blot

(Lin et al., 2015, Otani et al., 2015)

IKKε - nuf

enzymatic study, molecular weight estimation by staining, pull down, western blot

(Otani et al., 2011)

IKKε - sktl

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Jouette et al., 2019)

IKKε - spn-F

anti bait coimmunoprecipitation, western blot, surface plasmon resonance, anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry, enzymatic study, autoradiography

(Lu et al., 2020, Lin et al., 2015, Otani et al., 2015, Amsalem et al., 2013, Dubin-Bar et al., 2008)

RNA-RNA

Physical Interaction

Assay

References

IKKε - Cen

pull down, quantitative reverse transcription pcr

(Bergalet et al., 2020)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

IKKε

suppressible

ctp

(Otani et al., 2011)

IKKε

enhanceable

Dark

(Oshima et al., 2006)

IKKε

suppressible

Dhc64C

(Otani et al., 2011)

IKKε

enhanceable

Diap1

(Koto et al., 2009, Oshima et al., 2006)

IKKε

suppressible

Diap1

(Otani et al., 2011, Oshima et al., 2006)

IKKε

suppressible

Dronc

(Kuranaga et al., 2006)

IKKε

enhanceable

Dronc

(Koto et al., 2009)

IKKε

enhanceable

(Otani et al., 2011)

IKKε

suppressible

nuf

(Otani et al., 2011)

IKKε

enhanceable

Rab11

(Otani et al., 2011)

IKKε

suppressible

spn-F

(Lin et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

jvl

suppressible

IKKε

(Baskar et al., 2019)

par-1

enhanceable

IKKε

(Herzmann et al., 2017)

rpr

suppressible

IKKε

(Kuranaga et al., 2006)

spn-F

suppressible

IKKε

(Otani et al., 2015, Amsalem et al., 2013)

αTub67C

enhanceable

IKKε

(Barros and Bossing, 2021)

αTub67C

suppressible

IKKε

(Barros and Bossing, 2021)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61292

DroID - A comprehensive database of gene and protein interactions.

FBgn0086657

MIST (genetic) - An integrated Molecular Interaction Database

35329

MIST (protein-protein) - An integrated Molecular Interaction Database

35329

Pathways

Signaling Pathways (FlyBase)

CGAS-STING SIGNALING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q86PE7

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

38D4-38D5

Sequence location

2L:20,676,045..20,678,979 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

38D4-38D5

Limits computationally determined from genome sequence between P{lacW}k02501&P{lacW}k14810 and P{PZ}Hr38⁰²³⁰⁶&P{PZ}dia¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

38E1-38E5

(Inohara, 1999.10.22)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-54.1

(Comeron et al., 2012)

(Kozlova et al., 1998)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (26)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD10041

Other clones

FI05319

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086657

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086657

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Otani et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

ik2 protein is locally activated (by phosphorylation at residue Ser175) at the tip of growing mechanosensory bristles and controls the rapid shuttling of recycling endosomes. ik2 is also involved in actin bundle organisation. These two processes (recycling endosome dynamics and actin bundle organisation) appear to be independently regulated by ik2.

(Otani et al., 2011)

Mutations in ik2 affect polarized bristle microtubule formation.

(Bitan et al., 2010)

ik2 is required for dendritic severing during dendrite pruning of ddaC neurons in the pupa.

(Lee et al., 2009)

ik2 acts upstream of the Tl-dl pathway during oogenesis.

(Shapiro and Anderson, 2006)

Both the actin cytoskeleton and the anchoring of microtubule minus-ends are disrupted in ik2 mutants.

(Shapiro and Anderson, 2006)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities and spindle abnormalities are seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: ik2 l(2)38Ea

(Shapiro and Anderson, 2006)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ik2 CG2615

(Adams, 1999.11)

Source for identity of: IKKε ik2

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'ik2' to 'IKKε' to be more informative/consistent, and to represent usage in FBrf0193419, FBrf0194448 and FBrf0212985 etc.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (28)

Reported As

Symbol Synonym

38D.31

(Butler, 2001, Butler et al., 2001, McGill Drosophila Genome Project, 2001.9.25)

APTX7

(Kuranaga et al., 2006)

CG2615

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Sinka et al., 2008, Kuranaga et al., 2006, Oshima et al., 2006, Kanuka et al., 2005, Morrison et al., 2000)

DIK2

(Morrison et al., 2000)

Dik2

DmIKKε

(Yu and Pan, 2018, Nakajima and Kuranaga, 2017, Orme et al., 2016, Kuranaga, 2011, Miura, 2011, Koto et al., 2009, Bergmann, 2006, Kuranaga et al., 2006, Montell, 2006, Liao et al., 2002, Wasserman, 2000)

DmIKKε/dIK2

(Silverman and Maniatis, 2001)

Dmik2

(Lemaitre, 2000.12.20)

IK2

(Barros and Bossing, 2021, Lazzaro et al., 2004, Sun et al., 2002, Sun et al., 2002)

IKK

(Silverman et al., 2008)

IKKepsilon

(Gene Disruption Project members, 2018-, Zhang et al., 2018, Transgenic RNAi Project members, 2017-)

IKKε

(Ermanoska et al., 2023, Ishibashi et al., 2021, Lin et al., 2020, Baskar et al., 2019, Jouette et al., 2019, Meltzer et al., 2019, Otani et al., 2016, Otani et al., 2015, Gould, 2011, Otani et al., 2011, Oshima, 2006, Oshima et al., 2006)

Ik2

(Rui, 2024, Furusawa and Emoto, 2021, Bergalet et al., 2020, Lin et al., 2020, Amsalem et al., 2013, Bond and Foley, 2009, Caussinus et al., 2008, Bergmann, 2006, Wasserman, 2000)

Ik2/DmIKK

(Kuranaga et al., 2006)

dIKK

(Mushegian and Medzhitov, 2001)

dIKKε

(Goto et al., 2018)

dTBK1

(Abreha et al., 2021)

dik2

(Inohara, 1999.10.22)

ikkε

(Baskar et al., 2019, Otani et al., 2015)

l(2)38Ea

(Kozlova et al., 2009, Kuranaga et al., 2006, Oshima, 2006)

Name Synonyms

I-kappaB kinase ε

IKK-related kinase

(Dubin-Bar et al., 2008)

Ik2

(Gillingham et al., 2014)

IkappaB kinase-like 2

(Caussinus et al., 2008)

IκB kinase-like 2

(Shapiro and Anderson, 2006)

TANK-binding kinase 1

(Abreha et al., 2021)

apoptoxin 7

(Kuranaga et al., 2006)

ik2

lethal (2) 38Ea

Secondary FlyBase IDs

FBgn0025164
FBgn0028633
FBgn0043071

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35329

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3Y8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3Y8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086657

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35329/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086657

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086657

FlyMine - An integrated database for Drosophila genomics

FBgn0086657

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2615

MARRVEL_MODEL - MARRVEL (model organism gene)

35329

Linkouts

BioGRID - A database of protein and genetic interactions.

61292

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086657

DroID - A comprehensive database of gene and protein interactions.

FBgn0086657

DRSC - Results frm RNAi screens

FBgn0086657

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ik2_1
ik2_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086657

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086657

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2615

Flygut - An atlas of the Drosophila adult midgut

FBgn0086657

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086657

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

35329

MIST (protein-protein) - An integrated Molecular Interaction Database

35329

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q86PE7

References (122)

The following external sites may use different assemblies or annotations than FlyBase.

-0.11

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