FB2026_02 , released June 18, 2026
Allele: Dmel\IKKεG250D.UAS.Tag:MYC
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General Information
Symbol
Dmel\IKKεG250D.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0196644
Feature type
allele
Associated gene
Dmel\IKKε
Associated Insertion(s)
Carried in Construct
P{UAS-IKKε.G250D}
Also Known As
UAS-ik2-G250D
Key Links
Transgenic product class
missense_variant
(Kuranaga et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kuranaga et al., 2006)
Mutagen
in vitro construct
(Kuranaga et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-IKKε.G250D}
Cytology
Description

UASt regulatory sequences drive expression of full-length IKKε (derived from the GM07508 cDNA clone), mutated to carry a G250D amino acid replacement (this results in a 'kinase-dead' protein).

(Kuranaga et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
IKKε
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      dendrite & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.PG

      (Lee et al., 2009)

      dendrite | pupal stage, with Scer\GAL4ppk.PG

      (Lin et al., 2015)

      microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.PG

      (Lee et al., 2009)
      Detailed Description
      Statement
      Reference

      Overexpression of IKKεG250D.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4ppk.PG leads to dendrite pruning defects in pupae (16h after puparium formation).

      (Lin et al., 2015)

      The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2G250D.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ppk.PG, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).

      The microtubules remain intact in the dendrites of ddaC neurons at 6 hours after puparium formation (APF) in animals expressing ik2G250D.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ppk.PG (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage).

      (Lee et al., 2009)

      Expression of the kinase-dead ik2G250D.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PHb in S2 cells does not lead to an increase in cell death.

      Expression of ik2Scer\UAS.T:Hsap\MYC in flies, under the control of Scer\GAL4GMR.PF, does not cause the severe eye-ablation phenotype seen when wild-type ik2 is overexpressed.

      (Kuranaga et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of spn-F8D.Scer\UAS.T:Avic\GFP-EGFP does not suppress the dendrite pruning defects in pupae with IKKεG250D.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4ppk.PG.

      (Lin et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      IKKεG250D.Scer\UAS.T:Hsap\MYC
      IKKεG250D.UAS.Tag:MYC
      ik2G250D.Scer\UAS.T:Hsap\MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (5)