FlyBase Gene Report: Dmel\hpo

General Information

Symbol

Dmel\hpo

Species

D. melanogaster

Name

hippo

Annotation Symbol

CG11228

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261456

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

hippo (hpo) encodes a kinase in the Salvador-Warts-Hippo pathway. It controls tissue growth by controlling cell growth, proliferation and apoptosis. It has several roles in post-mitotic cells including fate specification of photoreceptors and tiling of dendritic neurons. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

dMST, MST

Key Links

Genomic Location

Cytogenetic map

56D13-56D13

Sequence location

2R:19,493,996..19,496,856 [-]

Recombination map

2-89

RefSeq locus

NT_033778 REGION:19493996..19496856

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (37 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Slmap; FB:FBgn0040011

(Zheng et al., 2017)

inferred from physical interaction with UniProtKB:Q9VFG8

(assigned by UniProt )

(Yu et al., 2010)

enables protein homodimerization activity

inferred from physical interaction with FLYBASE:hpo; FB:FBgn0261456

(Jin et al., 2012)

enables protein serine/threonine kinase activity

inferred from direct assay

(Zheng et al., 2017, Zheng et al., 2015, Jin et al., 2012, Wei et al., 2007, Colombani et al., 2006, Pantalacci et al., 2003, Wu et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000684825

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (28 terms)

Terms Based on Experimental Evidence (25 terms)

CV Term

Evidence

References

involved_in activation of cysteine-type endopeptidase activity

inferred from direct assay

(Jin et al., 2013)

involved_in border follicle cell migration

inferred from mutant phenotype

(Lucas et al., 2013)

involved_in compound eye morphogenesis

inferred from mutant phenotype

(Sarkar et al., 2018)

involved_in eye development

inferred from mutant phenotype

(Nolo et al., 2006)

involved_in hippo signaling

inferred from mutant phenotype

(Sun et al., 2019, Fletcher et al., 2018, Yu and Pan, 2018, Zhang et al., 2017, Fletcher et al., 2015, Wehr et al., 2013, Grzeschik et al., 2010, Harvey et al., 2003, Udan et al., 2003)

inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739

(Wei et al., 2007)

inferred from direct assay

(Zheng et al., 2015, Jin et al., 2012, Oh et al., 2009, Dong et al., 2007, Huang et al., 2005, Wu et al., 2003)

inferred from genetic interaction with FLYBASE:ft; FB:FBgn0001075

(Willecke et al., 2006)

inferred from mutant phenotype

(assigned by UniProt )

(Jahanshahi et al., 2016)

involved_in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

inferred from genetic interaction with FLYBASE:p53; FB:FBgn0039044

(Colombani et al., 2006)

involved_in morphogenesis of an epithelial sheet

inferred from mutant phenotype

(Thompson and Cohen, 2006)

involved_in negative regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(assigned by BHF-UCL )

(Varelas et al., 2010)

involved_in negative regulation of cell population proliferation

inferred from mutant phenotype

(Ding et al., 2016, Udan et al., 2003)

inferred from mutant phenotype

(assigned by UniProt )

(Wu et al., 2003)

involved_in negative regulation of glial cell proliferation

inferred from mutant phenotype

(Reddy and Irvine, 2011)

involved_in negative regulation of neuroblast proliferation

inferred from mutant phenotype

(Gil-Ranedo et al., 2019, Poon et al., 2016)

involved_in negative regulation of neuron apoptotic process

inferred from mutant phenotype

(Herz et al., 2006)

involved_in negative regulation of ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Aerne et al., 2015)

involved_in organ growth

inferred from direct assay

(Verghese et al., 2012)

involved_in peptidyl-threonine phosphorylation

inferred from direct assay

(Jin et al., 2012)

involved_in positive regulation of apoptotic process

inferred from mutant phenotype

(Pantalacci et al., 2003, Udan et al., 2003)

inferred from mutant phenotype

(assigned by UniProt )

(Wu et al., 2003)

involved_in protein autophosphorylation

inferred from direct assay

(Zheng et al., 2017, Jin et al., 2012)

involved_in protein phosphorylation

inferred from direct assay

(Wei et al., 2007)

inferred from direct assay

(assigned by UniProt )

(Wu et al., 2003)

involved_in R8 cell fate specification

inferred from mutant phenotype

(Mikeladze-Dvali et al., 2005)

involved_in regulation of apoptotic signaling pathway

inferred from mutant phenotype

(Nolo et al., 2006)

involved_in regulation of cell population proliferation

inferred from mutant phenotype

(Nolo et al., 2006)

involved_in regulation of protein processing

inferred from direct assay

(Jin et al., 2013)

involved_in response to ionizing radiation

inferred from mutant phenotype

(Colombani et al., 2006)

involved_in retinal cell programmed cell death

inferred from mutant phenotype

(Udan et al., 2003)

involved_in stem cell proliferation

inferred from direct assay

(Ren et al., 2010)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in hippo signaling

traceable author statement

(assigned by UniProt )

(Yu et al., 2010)

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN001969365

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN001969365

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein tetramerization

inferred from electronic annotation with InterPro:IPR036674

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

involved_in regulation of MAPK cascade

inferred from biological aspect of ancestor with PANTHER:PTN001969365

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in apicomedial cortex

inferred from direct assay

(Su et al., 2017)

colocalizes_with cell-cell junction

inferred from direct assay

(Su et al., 2017)

located_in cytosol

inferred from direct assay

(Su et al., 2017, Jin et al., 2012, Yu et al., 2010)

located_in membrane

inferred from direct assay

(assigned by UniProt )

(Yu et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000684825

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

GERMINAL CENTER KINASES

Pathway (FlyBase)

Hippo Signaling Pathway Core Components

Protein Family (UniProt)

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. (Q8T0S6)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

GERMINAL CENTER KINASES -: Germinal center kinases (GCKs) are serine/threonine kinases characterised by an N-terminal STE20 kinase domain. They have diverse roles in signaling pathways; some are MAPK kinase kinase kinases, activating MAPK kinase kinases. (Adapted from FBrf0225276 and FBrf0228279).

Pathway (FlyBase)

Hippo Signaling Pathway Core Components -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Activation of the Hippo pathway results in the down-regulation of cell proliferation and up-regulation of apoptosis, limiting tissue size. (Adapted from FBrf0224870).

Protein Function (UniProtKB)

Plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Phosphorylates Sav, Wts and Th/DIAP1. Regulates the level of Th/DIAP1 apoptosis inhibitor.

(UniProt, Q8T0S6)

Summary (Interactive Fly)

a serine/threonine kinase - acts in conjunction with Salvador and Warts to restrict cell growth - promotes cell death by decreasing the level of Thread, an inhibitor of apoptosis - polycomb genes interact with the tumor suppressor genes and in the maintenance of sensory neuron dendrites

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\hpo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8T0S6)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

hpo-RA

FBtr0086500

NM_137583

2503

669

hpo-RB

FBtr0331920

NM_001274163

2804

669

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

hpo-PA

FBpp0085688

75.1

669

4.95

FBpp0304253

75.1

669

4.95

Q8T0S6

NP_001261092

AGB93624

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

669 aa isoforms: hpo-PA, hpo-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer. Interacts with Sav and Wts. Interacts (via SARAH domain) with Ex. Interacts with Kibra.

(UniProt, Q8T0S6)

Post Translational Modification

Autophosphorylated.

(UniProt, Q8T0S6)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\hpo using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

hpo_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

third instar larval stage

imaginal disc

(Harvey et al., 2003)

Additional Descriptive Data

hpo transcript was detected in all imaginal discs with unpatterned expression.

(Harvey et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apicomedial cortex

inferred from direct assay

(Su et al., 2017)

colocalizes_with cell-cell junction

inferred from direct assay

(Su et al., 2017)

located_in cytosol

inferred from direct assay

(Su et al., 2017, Jin et al., 2012, Yu et al., 2010)

located_in membrane

inferred from direct assay

(assigned by UniProt )

(Yu et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\hpo in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\hpo

View exonic expression by tissue for Dmel\hpo

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261456

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37247/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261456

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034453

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261456

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11228

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 52 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of hpo

Transgenic constructs containing regulatory region of hpo

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)BSC782

(Kahsai et al., 2016)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^ci.PU

hpo^UAS.cHb

lethal, with Scer\GAL4^GMR.PF

hpo^UAS.cWa

lethal, with Scer\GAL4^GMR.PU

hpo^UAS.cHb

lethal, with Scer\GAL4^hh-Gal4

hpo^UAS.cHb

lethal (with Df(2R)BSC782)

hpo^KC202

lethal | recessive

lethal - all die before end of first instar larval stage

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage, with Scer\FLP1^ey.PU

hpo^{4A-431T.αTub84B.FRT.Tag:FLAG}

lethal - all die before end of pupal stage, with Scer\GAL4^GMR.PU

hpo^{UAS.WT.Tag:FLAG}

partially lethal - majority die | pupal stage | somatic clone

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

hpo^GD1570

viable, with Scer\GAL4^tin.CΔ4

hpo^GD1570

Sterility

Allele

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

fertile, with Scer\GAL4^VP16.nos.UTR

hpo^UAS.cUa

Other Phenotypes

Allele

abnormal cell death | somatic clone

hpo^KC202

abnormal cell growth

abnormal cell growth | somatic clone

hpo^KC202

abnormal cell migration

hpo^42-47

abnormal developmental rate | pharate adult stage, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

abnormal immune response | adult stage, with Scer\GAL4^r4

hpo^GD1570

abnormal immune response | adult stage, with Scer\GAL4^yolk

hpo^GD1570

abnormal mitotic cell cycle

hpo^KC202

abnormal mitotic cell cycle | somatic clone

abnormal mitotic cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

abnormal neuroanatomy

hpo^MGH4

abnormal neuroanatomy | adult stage | progressive | somatic clone

hpo^BF33

abnormal neuroanatomy | somatic clone | third instar larval stage

hpo^KC202

abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU, Scer\GAL80^ts.αTub84B

hpo^KK101704

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

hpo^KK101704

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

hpo^42-47

abnormal planar polarity | adult stage, with Scer\GAL4^ey.PH

hpo^KK101704

abnormal size | adult stage

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

hpo^UAS.cWa

abnormal size | adult stage, with Scer\GAL4^cv-c-C5

hpo^UAS.cUa

abnormal size | adult stage, with Scer\GAL4^en-e16E

hpo^dsRNA.UAS

abnormal size | adult stage, with Scer\GAL4^nub.PU

abnormal size | adult stage, with Scer\GAL4^unspecified

hpo^UAS.cUa

abnormal size | larval stage, with Scer\GAL4^dpp.PU

hpo^UAS.cUa

abnormal size | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU, Scer\GAL80^ts.αTub84B

hpo^KK101704

abnormal size | third instar larval stage, with Scer\GAL4^hh-Gal4

hpo^KK101704

abnormal size | third instar larval stage, with Scer\GAL4^insc-Mz1407

hpo^KK101704

abnormal size | third instar larval stage, with Scer\GAL4^ptc-559.1

hpo^KK101704

abnormal stress response | somatic clone

hpo^42-47

atrophy | pupal stage, with Scer\GAL4^Act88F.PB

hpo^UAS.cUa

decreased cell death | larval stage | somatic clone

hpo^KC202

decreased cell death | pupal stage | somatic clone

hpo^42-47

decreased cell death | somatic clone

decreased cell death | somatic clone, with Scer\GAL4^Act5C.PP

hpo^dsRNA.UAS

decreased cell number | somatic clone, with Scer\GAL4^Act5C.PP

hpo^UAS.cPa

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

hpo^{UAS.WT.Tag:FLAG}

decreased occurrence of cell division | larval stage, with Scer\GAL4^cv-c-C5

hpo^UAS.cUa

decreased size | pupal stage, with Scer\GAL4^Mef2.PR

hpo^KK101704

decreased size | pupal stage | somatic clone - tissue specific

hpo^{4A-431T.αTub84B.FRT.Tag:FLAG}

decreased size | third instar larval stage, with Scer\GAL4^nub.PU

hpo^G3315

flightless, with Scer\GAL4^Mef2.PR

hpo^KK101704

hyperactive | adult stage

hpo^KC202

hyperplasia, with Scer\GAL4^GMR.PU

hpo^GD1570

hyperplasia | adult stage | somatic clone

hpo^42-47

hyperplasia | somatic clone

hpo^42-47

hypoactive | adult stage

hpo^KS240

increased cell death, with Scer\GAL4^cv-c-C5

hpo^UAS.cUa

increased cell death, with Scer\GAL4^GMR.PF

increased cell death, with Scer\GAL4^hs.PB

hpo^UAS.cUa

increased cell death, with Scer\GAL4^nub-AC-62

hpo^Δauto.UAS

increased cell death | larval stage, with Scer\GAL4^Bx-MS1096

hpo^UAS.cWa

increased cell death | larval stage, with Scer\GAL4^cv-c-C5

hpo^UAS.cUa

increased cell number, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

hpo^HMS00006

increased cell number | adult stage | somatic clone

hpo^MGH1

increased cell number | cell non-autonomous | somatic clone

hpo^BF33

increased cell number | pupal stage, with Scer\GAL4^αTub84B.PL

hpo^dsRNA.UAS

increased cell number | pupal stage | somatic clone

increased cell number | somatic clone

increased cell number | somatic clone, with Scer\GAL4^Act5C.PP

hpo^dsRNA.UAS

increased cell number | third instar larval stage, with Scer\GAL4^repo.PU

hpo^KK101704

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

increased occurrence of cell division | adult stage | cell non-autonomous | somatic clone

hpo^BF33

increased occurrence of cell division | adult stage | somatic clone

hpo^BF33

increased occurrence of cell division | pupal stage | somatic clone

hpo^42-47

increased occurrence of cell division | somatic clone

increased size | adult stage, with Scer\GAL4^en-e16E

hpo^JF02740

increased size | adult stage, with Scer\GAL4^GMR.PU

hpo^KK101704

increased size | adult stage, with Scer\GAL4^nub.PU

hpo^HMS00006

increased size | adult stage, with Scer\GAL4^ptc.PU

hpo^KK101704

melanotic mass phenotype | adult stage, with Scer\GAL4^pnr-MD237

hpo^KK101704

neoplasia | somatic clone

some die during first instar larval stage

some die during larval stage | recessive

some die during pupal stage, with Scer\GAL4^GMR.PU

hpo^{UAS.WT.Tag:FLAG}

visible

hpo^GMR.PW

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^C-765

visible, with Scer\GAL4^ci.PU

hpo^S30A.UAS

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^GMR.PU

hpo^GD1570

visible, with Scer\GAL4^nub.PU

hpo^UAS.cUa

visible, with Scer\GAL4^nub-AC-62

hpo^UAS.cUa

visible, with Scer\GAL4^pnr-MD237

hpo^GD1570

visible | adult stage

hpo^GMR.PW

visible | adult stage, with Scer\GAL4^en-e16E

visible | adult stage, with Scer\GAL4^GMR.PU

hpo^KK101704

visible | adult stage, with Scer\GAL4^nub.PU

hpo^HMS00006

visible | adult stage, with Scer\GAL4^pnr-MD237

hpo^KK101704

visible | adult stage, with Scer\GAL4^ptc.PU

hpo^KK101704

visible | adult stage | somatic clone

visible | recessive

visible | somatic clone

Phenotype manifest in

Allele

adult cuticle | somatic clone

hpo^42-47

adult external abdomen, with Scer\GAL4^pnr-MD237

hpo^KK101704

adult external head | somatic clone

adult head, with Scer\GAL4^cv-c-C5

hpo^UAS.cUa

adult head | somatic clone

adult midgut, with Scer\GAL4^NP5130

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

hpo^KK101704

border follicle cell

hpo^42-47

embryonic/larval brain | third instar larval stage, with Scer\GAL4^repo.PU

hpo^KK101704

embryonic/larval central nervous system | third instar larval stage, with Scer\GAL4^insc-Mz1407

hpo^KK101704

embryonic/larval glial cell | third instar larval stage, with Scer\GAL4^repo.PU

hpo^KK101704

eye

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PU

eye | adult stage | somatic clone

eye | P-stage | somatic clone

hpo^42-47

eye | pupal stage | somatic clone, with Scer\GAL4^αTub84B.PL

hpo^dsRNA.UAS

eye | somatic clone

eye | temperature conditional, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

eye-antennal disc, with Scer\GAL4^Act5C.PP

eye-antennal disc, with Scer\GAL4^GMR.PF

hpo^UAS.cPa

eye disc, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

eye disc | posterior | somatic clone

hpo^42-47

eye disc | somatic clone

hpo^BF33

eye disc | third instar larval stage, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

eye disc | third instar larval stage, with Scer\GAL4^repo.PU

hpo^KK101704

eye photoreceptor cell | progressive | somatic clone

hpo^BF33

haltere

hpo^KC202

haltere, with Scer\GAL4^Act5C.PP

hpo^dsRNA.UAS

head & cuticle

imaginal disc, with Scer\GAL4^Act5C.PP

hpo^dsRNA.UAS

indirect flight muscle cell, with Scer\GAL4^Act88F.PB

hpo^UAS.cUa

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

hpo^KK101704

indirect flight muscle cell | pupal stage | somatic clone - tissue specific

hpo^{4A-431T.αTub84B.FRT.Tag:FLAG}

interommatidial bristle, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

interommatidial bristle | somatic clone

hpo^MGH1

interommatidial bristle | somatic clone | increased number

hpo^BF33

interommatidial cell | pupal stage, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

interommatidial cell | pupal stage, with Scer\GAL4^αTub84B.PL

hpo^dsRNA.UAS

interommatidial cell | pupal stage | increased number

hpo^42-47

interommatidial cell | pupal stage | somatic clone | increased number

hpo^5.1

interommatidial cell | somatic clone | increased number

intestinal stem cell | somatic clone

hpo^BF33

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

hpo^{UAS.WT.Tag:FLAG}

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

hpo^HMS00006

larval dorsal multidendritic neuron ddaC | somatic clone

hpo^MGH4

larval ventral nerve cord | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU, Scer\GAL80^ts.αTub84B

hpo^KK101704

leg

hpo^KC202

mesothoracic tergum, with Scer\GAL4^pnr-MD237

hpo^GD1570

mitotic spindle | somatic clone | third instar larval stage

hpo^KC202

myotube, with Scer\GAL4^Act88F.PB

hpo^UAS.cUa

myotube | pupal stage, with Scer\GAL4^Mef2.PR

hpo^KK101704

myotube | pupal stage | somatic clone - tissue specific

hpo^{4A-431T.αTub84B.FRT.Tag:FLAG}

NMJ bouton | increased number, with Scer\GAL4^RapGAP1-OK6

ommatidium

hpo^GMR.PW

ommatidium, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

ommatidium | adult stage, with Scer\GAL4^ey.PH

hpo^KK101704

ommatidium | somatic clone

photoreceptor cell R8 of pale ommatidium | increased number

hpo^KC203

photoreceptor cell R8 of pale ommatidium | increased number, with Scer\GAL4^GMR.long

hpo^K71R.UAS

photoreceptor cell R8 of yellow ommatidium

hpo^KC203

photoreceptor cell R8 of yellow ommatidium, with Scer\GAL4^GMR.long

hpo^K71R.UAS

photoreceptor neuron

hpo^KC202

photoreceptor neuron, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

pigment cell, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

pigment cell | ectopic | somatic clone

hpo^42-47

posterior crossvein, with Scer\GAL4^en-e16E

hpo^JF02740

retina, with Scer\GAL4^GMR.PF

hpo^UAS.cUa

retina | P-stage | somatic clone

thorax

hpo^KC202

type II neuroblast | somatic clone | third instar larval stage

hpo^KC202

wing

wing, with Scer\GAL4^Bx-MS1096

wing, with Scer\GAL4^C-765

wing, with Scer\GAL4^ci.PU

hpo^S30A.UAS

wing, with Scer\GAL4^cv-c-C5

hpo^UAS.cUa

wing, with Scer\GAL4^en-e16E

hpo^JF02740

wing, with Scer\GAL4^nub.PU

wing, with Scer\GAL4^nub-AC-62

hpo^UAS.cUa

wing | posterior, with Scer\GAL4^en-e16E

hpo^dsRNA.UAS

wing basal cell 1, with Scer\GAL4^ptc.PU

hpo^KK101704

wing disc, with Scer\GAL4^Bx-MS1096

hpo^UAS.cWa

wing disc, with Scer\GAL4^dpp.PU

hpo^UAS.cUa

wing disc, with Scer\GAL4^hs.PB

hpo^UAS.cUa

wing disc, with Scer\GAL4^nub-AC-62

hpo^Δauto.UAS

wing disc | larval stage | somatic clone

hpo^42-47

wing disc | somatic clone

hpo^42-48

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

hpo^UAS.Tag:FLAG

wing disc | somatic clone, with Scer\GAL4^unspecified

hpo^UAS.cPa

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^hh-Gal4

hpo^KK101704

wing hinge primordium, with Scer\GAL4^sd-SG29.1

hpo^dsRNA.UAS

wing pouch | third instar larval stage, with Scer\GAL4^nub.PU

hpo^G3315

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\STK3

13 of 15

Yes

Hsap\STK4

11 of 15

Yes

Hsap\STK24

2 of 15

Hsap\STK25

2 of 15

Hsap\STK26

2 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Stk3

14 of 15

Yes

Mmus\Stk4

11 of 15

Yes

Mmus\Stk24

2 of 15

Mmus\Stk26

2 of 15

Mmus\Stk25

1 of 15

Rattus norvegicus (Norway rat) (7)

Rnor\Stk3

11 of 13

Yes

Rnor\Stk4

9 of 13

Yes

Rnor\LOC108349780

2 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\stk3

9 of 12

Yes

Xtro\stk4

9 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

Yes

Xtro\LOC100490567

1 of 12

Yes

Xtro\LOC100492522

1 of 12

Yes

Xtro\LOC100495971

1 of 12

Yes

Xtro\LOC101731773

1 of 12

Yes

Xtro\LOC108648113

1 of 12

Yes

Danio rerio (Zebrafish) (8)

Drer\stk3

14 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\cst-1

14 of 15

Yes

Cele\cst-2

14 of 15

Yes

Cele\gck-1

2 of 15

Cele\C24A8.6

1 of 15

Yes

Cele\dop-6

1 of 15

Cele\flr-4

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (5)

Atha\SIK1

5 of 9

Yes

Atha\AT3G15220

1 of 9

Atha\AT4G14350

1 of 9

Atha\AT5G16500

1 of 9

Yes

Atha\ATMAP4K ALPHA1

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\SPS1

2 of 15

Yes

Scer\KIC1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\gad8

1 of 12

Yes

Spom\nak1

1 of 12

Yes

Spom\ppk11

1 of 12

Yes

Spom\sid1

1 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (15)

Gene Symbol

Score

Source

Alignment

4 of 10

2 of 10

2 of 10

mbt

2 of 10

msn

2 of 10

Pak

2 of 10

Slik

2 of 10

Stlk

2 of 10

Tao

2 of 10

Dsor1

1 of 10

hep

1 of 10

lic

1 of 10

Mekk1

1 of 10

Mkk4

1 of 10

Pak3

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

hpo^42-47

model of cancer

CEA

(Hamaratoglu et al., 2006)

hpo^BF33

model of neurodegenerative disease

CEA

(Napoletano et al., 2011)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

hpo^KS240

exacerbates autism spectrum disorder

modeled by Abca3^GD3708

(Ueoka et al., 2020)

hpo^3D

exacerbates carcinoma

modeled by Vps25^N55

(Herz et al., 2006)

hpo^42-47

ameliorates gastrointestinal adenoma

modeled by Apc^Q8, Apc2^g10

(Suijkerbuijk et al., 2016)

hpo^UAS.cUa

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Irwin et al., 2020)

hpo^dsRNA.UAS

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Irwin et al., 2020)

hpo^KC202

exacerbates autism spectrum disorder

modeled by Abca3^GD3708

(Ueoka et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

STK3; serine/threonine kinase 3

13 of 15

605030

STK4; serine/threonine kinase 4

11 of 15

604965

T-CELL IMMUNODEFICIENCY, RECURRENT INFECTIONS, AND AUTOIMMUNITY WITH OR WITHOUT CARDIAC MALFORMATIONS; TIIAC

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

hpo

Hsap\STK3

(Wu et al., 2003)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

hpo - Arf1

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Kwon et al., 2013, Kwon et al., 2013)

hpo - CCT2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - CYLD

anti tag coimmunoprecipitation, anti tag western blot

(Chen et al., 2014)

hpo - Cka

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting, western blot

(Zheng et al., 2017, Ribeiro et al., 2010)

hpo - Dap160

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - Diap1

enzymatic study, autoradiography

(Harvey et al., 2003, Pantalacci et al., 2003)

hpo - Eps-15

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - Fgop2

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Zheng et al., 2017, Ribeiro et al., 2010)

hpo - Git

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Dent et al., 2015)

hpo - Herc4

anti tag coimmunoprecipitation, anti tag western blot

(Aerne et al., 2015)

hpo - Mer

anti tag coimmunoprecipitation, anti tag western blot

(Reddy and Irvine, 2011)

hpo - Mob4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Ribeiro et al., 2010)

hpo - Pp2A-29B

anti tag coimmunoprecipitation, peptide massfingerprinting

(Ribeiro et al., 2010)

hpo - Ran

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - Rap1

pull down, anti tag western blot, enzymatic study, western blot

(Chang et al., 2018)

hpo - Rassf

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, pull down, molecular weight estimation by staining, mass spectrometry studies of complexes, anti bait coimmunoprecipitation, western blot

(Cairns et al., 2020, Dent et al., 2015, Kwon et al., 2013, Jin et al., 2012, Ribeiro et al., 2010, Polesello et al., 2006)

hpo - RpS18

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - RtGEF

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Dent et al., 2015)

hpo - SERCA

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - Slmap

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based

(Zheng et al., 2017, Guruharsha et al., 2011)

hpo - Strip

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Sakuma et al., 2016, Ribeiro et al., 2010)

hpo - Tao

protein kinase assay, anti tag western blot

(Boggiano et al., 2011)

hpo - ci

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2015)

hpo - ds

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Kwon et al., 2013, Kwon et al., 2013)

hpo - ed

anti tag coimmunoprecipitation, anti tag western blot

(Yue et al., 2012)

hpo - ex

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Reddy and Irvine, 2011, Yu et al., 2010)

hpo - kibra

anti tag coimmunoprecipitation, western blot

(Yu et al., 2010)

hpo - mEFTu1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

hpo - mats

enzymatic study, western blot, pull down, autoradiography

(Zheng et al., 2017, Zheng et al., 2015, Jin et al., 2012)

hpo - mts

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Gil-Ranedo et al., 2019, Zheng et al., 2017, Ribeiro et al., 2010)

hpo - par-1

anti tag coimmunoprecipitation, anti tag western blot

(Huang et al., 2013)

hpo - sav

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, pull down, anti bait coimmunoprecipitation, western blot, molecular weight estimation by staining, autoradiography, x-ray crystallography, tandem affinity purification, molecular sieving, two hybrid, enzymatic study, coimmunoprecipitation, mass spectrometry studies of complexes, protein cross-linking with a bifunctional reagent, molecular weight

hpo - slmb

anti tag coimmunoprecipitation, anti tag western blot

(Tokamov et al., 2021)

hpo - trc

anti tag coimmunoprecipitation, anti tag western blot, western blot, enzymatic study, autoradiography

(Emoto et al., 2006)

hpo - wts

enzymatic study, autoradiography, anti tag coimmunoprecipitation, anti tag western blot, western blot

(Li et al., 2015, Zheng et al., 2015, Yin et al., 2013, Oh et al., 2009, Emoto et al., 2006, Jia et al., 2003, Pantalacci et al., 2003, Wu et al., 2003)

hpo - yki

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Srivastava et al., 2021, Dent et al., 2015, Oh et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Hu et al., 2016)

suppressible

(Jin et al., 2013)

suppressible

(Nie et al., 2015)

enhanceable

(Verghese et al., 2012)

hpo

enhanceable

Dark|Dronc

(Verghese et al., 2012)

hpo

suppressible

Diap1

(Udan et al., 2003)

suppressible

(Ren et al., 2010)

enhanceable

(Verghese et al., 2012)

hpo

suppressible

Dronc

(Verghese et al., 2012)

suppressible

(Ren et al., 2010)

suppressible

(Lucas et al., 2013)

hpo

enhanceable

Git

(Dent et al., 2015)

hpo

enhanceable

Git|RtGEF

(Dent et al., 2015)

hpo

suppressible

hid

(Udan et al., 2003)

hpo

suppressible

Hrb27C

(Mach et al., 2018)

hpo

suppressible

Leash

(Kwon et al., 2013)

hpo

enhanceable

Leash

(Kwon et al., 2013)

suppressible

(Nie et al., 2015)

suppressible

(Nolo et al., 2006)

hpo

suppressible

Mnat9

(Mok and Choi, 2022)

hpo

enhanceable

Mnat9

(Mok and Choi, 2022)

hpo

suppressible

Ncoa6

(Qing et al., 2014)

hpo

enhanceable

Rassf

(Polesello et al., 2006)

suppressible

(Guo et al., 2020)

enhanceable

(Dent et al., 2015)

hpo

enhanceable

sav

(Wu et al., 2003)

hpo

suppressible

(Zhang et al., 2008)

hpo

suppressible

Smox

(Hevia et al., 2017)

hpo

suppressible

syd

(Ahmad et al., 2021)

hpo

enhanceable

Tao

(Poon et al., 2011)

hpo

suppressible

Tao

(Poon et al., 2011)

hpo

suppressible

Tgi

(Guo et al., 2013, Koontz et al., 2013)

hpo

suppressible

trc

(Emoto et al., 2006)

hpo

suppressible

trr

(Qing et al., 2014)

hpo

suppressible

Tsc1

(Udan et al., 2003)

hpo

enhanceable

Uba1

(Pfleger et al., 2007)

hpo

suppressible

wts

(Yin et al., 2013, Hamaratoglu et al., 2006, Udan et al., 2003)

suppressible

(Li et al., 2015)

enhanceable

(Jia et al., 2003, Wu et al., 2003)

hpo

suppressible

yki

(Verghese et al., 2012, Ren et al., 2010, Oh and Irvine, 2008, Zhao et al., 2007, Huang et al., 2005)

suppressible

(Li et al., 2015)

suppressible

(Huang et al., 2005)

hpo

suppressible

Zyx

(Gaspar et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Abca3

suppressible

hpo

(Ueoka et al., 2020)

Abca3

enhanceable

hpo

(Ueoka et al., 2020)

Acn

suppressible

hpo

(Tyra et al., 2020)

Alg3

suppressible

hpo

(de Vreede et al., 2018)

Apc2|Apc

suppressible

hpo

(Suijkerbuijk et al., 2016)

enhanceable

hpo

(Li et al., 2015)

suppressible

(Li et al., 2015)

suppressible

(Wu et al., 2003)

suppressible

(Sansores-Garcia et al., 2011)

suppressible

hpo

(Hamaratoglu et al., 2006)

grim

suppressible

hpo

(Harvey et al., 2003)

enhanceable

hpo

(Li et al., 2015)

hid

suppressible

hpo

(Jia et al., 2003, Pantalacci et al., 2003, Udan et al., 2003)

enhanceable

(Jia et al., 2003)

suppressible

(Genevet et al., 2010, Genevet et al., 2010)

suppressible

(Li et al., 2017)

suppressible

(Mok and Choi, 2022)

p53

suppressible

hpo

(Colombani et al., 2006)

enhanceable

hpo

(Parrish et al., 2007)

Pez

suppressible

hpo

(Poernbacher et al., 2012)

Rassf

suppressible

hpo

(Polesello et al., 2006)

Smox

suppressible

hpo

(Hevia et al., 2017)

Strip

suppressible

hpo

(Sakuma et al., 2016)

syd

suppressible

hpo

(Ahmad et al., 2021)

Vps25

suppressible

hpo

(Herz et al., 2006)

suppressible

(Oh et al., 2009)

enhanceable

(Jin et al., 2012)

suppressible

(Yeom et al., 2020, Oh and Irvine, 2009)

suppressible

(Oh et al., 2009)

suppressible

(Oh and Irvine, 2009, Oh et al., 2009)

External Data

Subunit Structure (UniProtKB)

Homodimer. Interacts with Sav and Wts. Interacts (via SARAH domain) with Ex. Interacts with Kibra.

(UniProt, Q8T0S6 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261456

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Hippo Signaling Pathway Core Components -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Activation of the Hippo pathway results in the down-regulation of cell proliferation and up-regulation of apoptosis, limiting tissue size. (Adapted from FBrf0224870).

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-89

Cytogenetic map

56D13-56D13

Sequence location

2R:19,493,996..19,496,856 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56D13-56D13

Limits computationally determined from genome sequence between P{lacW}l(2)k00705^k00705&P{lacW}mei-W68^k05603 and P{EP}CG11007^EP736

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-89

(FlyBase, 2016)

2-94.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27661

y¹ v¹; P{TRiP.JF02740}attP2

33614

y¹ v¹; P{TRiP.HMS00006}attP2

27105

y¹ w^*; P{EP}hpo^G3315

44254

y¹ w^*; wg^Sp-1/CyO; P{UAS-dMST.FLAG}3/TM2

25085

P{hsFLP}12, y¹ w^*; P{neoFRT}42D hpo^KS240/CyO

35176

y¹ sc^* v¹ sev²¹; P{TRiP.GL00046}attP2

85878

y¹ sc^* v¹ sev²¹; P{TKO.GS04500}attP40

25090

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D hpo^KC202/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

VDRC

v104169

P{KK101704}VIE-260B

v7823

w¹¹¹⁸; P{GD1570}v7823

v318388

PBac{fTRG10130.sfGFP-FT}VK00033

v341346

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00401}attP40/CyO-GFP

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (157)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH10354

Other clones

DPiM_GH10354

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261456

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261456

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37247

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Su et al., 2017, Wang et al., 2016, Jin et al., 2012)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: hippo CG11228

(Wu et al., 2003)

Source for merge of: hpo MST

(Zhang et al., 2008)

Additional comments

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Colombani et al., 2006, Polesello et al., 2006)

hpo acts cell autonomously in class IV neurons.

(Emoto et al., 2006)

hpo acts through trc and wts to regulate dendritic tiling and maintenance, respectively.

(Emoto et al., 2006)

hpo regulates expression of the ban miRNA.

(Nolo et al., 2006)

dsRNA made from templates generated with primers directed against this gene is tested in an S2 cell phosphorylation experiment to assess teh hpo signal transduction pathway.

(Willecke et al., 2006)

yki is a kinase substrate of wts, becoming phosphorylated by wts upon activation of the hpo pathway.

(Huang et al., 2005)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, spindle abnormalities and lagging chromatids are seen.

(Bettencourt-Dias et al., 2004)

Origin and Etymology

Discoverer

Etymology

Named "hippo" because of the dark, folded and overgrown cuticle of the mutant heads.

(Udan et al., 2003)

Identification

External Crossreferences and Linkouts ( 51 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37247

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8T0S6

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8T0S6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KFW0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8T0S6

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261456

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261456

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37247/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261456

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

hpo_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034453

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261456

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11228

FlyMine - An integrated database for Drosophila genomics

FBgn0261456

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

92399

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37247

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004606

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG11228

MARRVEL_MODEL - MARRVEL (model organism gene)

37247

modMine - A data warehouse for the modENCODE project

FBgn0261456

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8T0S6

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261456

DRSC - Results frm RNAi screens

FBgn0261456

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

HPO

(Ferrandon, 2013, Gonzalez, 2013, Grifoni et al., 2013, Harvey and Hariharan, 2012)

Hpo

(Bailey et al., 2021, Cho and Jiang, 2021, Srivastava et al., 2021, Yamaguchi et al., 2021, Chen et al., 2020, De Jamblinne et al., 2020, Fulford and McNeill, 2020, Hao et al., 2020, Jang et al., 2020, Mohammadi et al., 2020, Sahu and Mondal, 2020, Yeom et al., 2020, Borreguero-Muñoz et al., 2019, Chang et al., 2019, Fulford et al., 2019, Gokhale and Pfleger, 2019, Matsui and Lai, 2019, Politano et al., 2019, Bae and Luo, 2018, Bohère et al., 2018, Cairns et al., 2018, Cho et al., 2018, de Vreede et al., 2018, Elbediwy and Thompson, 2018, Fulford et al., 2018, Gou et al., 2018, Kim and Jho, 2018, Simón-Carrasco et al., 2018, Jang et al., 2017, Kulaberoglu et al., 2017, Liu and Jin, 2017, Ma et al., 2017, Richardson and Portela, 2017, Tue et al., 2017, Bhadra et al., 2016, Fallahi et al., 2016, Hu et al., 2016, Jahanshahi et al., 2016, Jiang et al., 2016, Meng et al., 2016, Saadin and Starz-Gaiano, 2016, Aerne et al., 2015, Cao et al., 2015, Dent et al., 2015, Di Cara et al., 2015, Ni et al., 2015, Thompson and Sahai, 2015, Amoyel and Bach, 2014, Chen et al., 2014, Sadeqzadeh et al., 2014, Andersen et al., 2013, Degoutin et al., 2013, Deng et al., 2013, Enderle and McNeill, 2013, Jin et al., 2013, Johnston, 2013, Koontz et al., 2013, Losick et al., 2013, Matsui and Lai, 2013, Rister et al., 2013, Vilmos et al., 2013, Wu and Wu, 2013, Yin et al., 2013, Yu and Guan, 2013, Fausti et al., 2012, Jin et al., 2012, Kango-Singh, 2012.10.31, Liu et al., 2012, Yue et al., 2012, Chan et al., 2011, Genevet and Tapon, 2011, Halder and Johnson, 2011, Laprise, 2011, Losick et al., 2011, Richter et al., 2011, Salah and Aqeilan, 2011, Zhang et al., 2011, Zhao et al., 2011, Yu et al., 2010, Badouel et al., 2009, Hamaratoglu et al., 2009, Koike-Kumagai et al., 2009, Oh and Irvine, 2009, Zhang et al., 2009, Dutta and Baehrecke, 2008, Jukam and Claude, 2008, Zhai et al., 2008, Dong et al., 2007, Hamaratoglu et al., 2007, Jukam and Desplan, 2007, Martin et al., 2007, Polesello and Tapon, 2007, Emoto et al., 2006, Hariharan, 2006, Polesello et al., 2006, Silva, 2006, Silva et al., 2006, Formstecher et al., 2005)

Hpo/Wts

(Jasper, 2020)

MST

(Kanao et al., 2012)

MST2

(Morrison et al., 2000)

Mst1/2

(van Soldt and Cardoso, 2020)

dMST

(Zhang et al., 2008, Jia et al., 2004, Jia et al., 2003)

hipo

(Kanao et al., 2012)

hippo

(Gong et al., 2021, Ng et al., 2016, Fletcher et al., 2015, Portela et al., 2015, Wittkorn et al., 2015, Ferguson and Martinez-Agosto, 2014, Ilanges et al., 2013)

hpo

(Ding et al., 2022, Agrawal et al., 2021, Badmos et al., 2021, Deshpande et al., 2021, Ding et al., 2021, Gogia et al., 2021, Harrison et al., 2021, Lebo et al., 2021, Liguori et al., 2021, Pojer et al., 2021, Sang et al., 2021, Tokamov et al., 2021, Yamaguchi et al., 2021, Yang and Choi, 2021, Baker, 2020, Bakshi and Joshi, 2020, Cairns et al., 2020, Gogia et al., 2020, Guo et al., 2020, Irwin et al., 2020, Kamdem et al., 2020, Neal et al., 2020, Ueoka et al., 2020, Yatsenko et al., 2020, Yu et al., 2020, Zhang et al., 2020, Arbouzova et al., 2019, Chatterjee and Deng, 2019, Coelho and Moreno, 2019, Fahey-Lozano et al., 2019, Herrera and Bach, 2019, Jiménez-Martínez et al., 2019, Khezri and Rusten, 2019, Luhur et al., 2019, Meltzer et al., 2019, Schaub et al., 2019, Snigdha et al., 2019, Sun et al., 2019, Xu et al., 2019, Yamamoto et al., 2019, Yee et al., 2019, Azuma et al., 2018, Forest et al., 2018, Kushimura et al., 2018, Li et al., 2018, Reilein et al., 2018, Sarkar et al., 2018, Tsuboi et al., 2018, Wang et al., 2018, Yu and Pan, 2018, Baker, 2017, Hevia et al., 2017, Li et al., 2017, Moeller et al., 2017, Neisch et al., 2017, Rossi et al., 2017, Su et al., 2017, Transgenic RNAi Project members, 2017-, Zheng et al., 2017, Boone et al., 2016, Chung et al., 2016, Huang et al., 2016, Kuleesha et al., 2016, Levinson and Cagan, 2016, Liu et al., 2016, Losick et al., 2016, Mao et al., 2016, Poon et al., 2016, Sakuma et al., 2016, Sarov et al., 2016, Suijkerbuijk et al., 2016, Dent et al., 2015, Di Cara et al., 2015, Dong et al., 2015, Enomoto et al., 2015, Fischer et al., 2015, Gailite et al., 2015, Gaspar et al., 2015, Keder et al., 2015, Kwon et al., 2015, Li et al., 2015, Meng et al., 2015, Nie et al., 2015, Wang and Baker, 2015, Wittkorn et al., 2015, Zhang et al., 2015, Zheng et al., 2015, Djabrayan et al., 2014, Huang and Kalderon, 2014, Hu et al., 2014, Li et al., 2014, Lin et al., 2014, Qing et al., 2014, Robbins et al., 2014, Sopko et al., 2014, Tipping and Perrimon, 2014, Vo et al., 2014, Chen et al., 2013, Deng et al., 2013, Guo et al., 2013, Huang et al., 2013, Ilanges et al., 2013, Jukam et al., 2013, Jukam et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Lucas et al., 2013, Marcinkevicius and Zallen, 2013, Southall et al., 2013, Wehr et al., 2013, Yin et al., 2013, Denton et al., 2012, Herranz et al., 2012, Justiniano et al., 2012, Papatheodorou et al., 2012, Poernbacher et al., 2012, Verghese et al., 2012, Ye et al., 2012, Bao et al., 2011, Boggiano et al., 2011, Chen et al., 2011, Fernández et al., 2011, Jukam and Desplan, 2011, Laprise, 2011, Matsubara et al., 2011, Napoletano et al., 2011, Poon et al., 2011, Rauskolb et al., 2011, Reddy and Irvine, 2011, Zhang et al., 2011, Baumgartner et al., 2010, Baumgartner et al., 2010, Chen et al., 2010, Das Thakur et al., 2010, Fang and Adler, 2010, Genevet et al., 2010, Genevet et al., 2010, Grzeschik et al., 2010, Ho et al., 2010, Milton et al., 2010, Neto-Silva et al., 2010, Nicolay et al., 2010, Ren et al., 2010, Ren et al., 2010, Ribeiro et al., 2010, Shaw et al., 2010, Varelas et al., 2010, Yu et al., 2010, Zecca and Struhl, 2010, Zeng et al., 2010, Cruz et al., 2009, Davidson et al., 2009, Genevet et al., 2009, Hamaratoglu et al., 2009, Oh et al., 2009, Zhang et al., 2009, Dutta and Baehrecke, 2008, Goulev et al., 2008, Nicolay and Frolov, 2008, Oh and Irvine, 2008, Sun et al., 2008, Willecke et al., 2008, Wu et al., 2008, Yu et al., 2008, Zhang et al., 2008, Feng and Irvine, 2007, Meignin et al., 2007, Parrish et al., 2007, Pellock et al., 2007, Pfleger et al., 2007, Polesello and Tapon, 2007, Soba et al., 2007, Tyler and Baker, 2007, Tyler et al., 2007, Wei et al., 2007, Wei et al., 2007, Zhao et al., 2007, Bennett and Harvey, 2006, Cho et al., 2006, Colombani et al., 2006, Edgar, 2006, Hamaratoglu et al., 2006, Polesello et al., 2006, Thompson and Cohen, 2006, Willecke et al., 2006, Mikeladze-Dvali et al., 2005, Udan et al., 2003)

Name Synonyms

HIPPO

(Liu et al., 2017)

Hippo

(Ito and Igaki, 2021, Lim et al., 2021, Paraskevopoulos and McGuigan, 2021, Chen and Desplan, 2020, Chen et al., 2020, Ding et al., 2020, Texada et al., 2020, van Soldt and Cardoso, 2020, Matsui and Lai, 2019, Mirzoyan et al., 2019, Cairns et al., 2018, Enomoto et al., 2018, Fletcher et al., 2018, Kim and Jho, 2018, Moon et al., 2018, Stuelten et al., 2018, Takeda et al., 2018, Hergovich, 2017, Bonfini et al., 2016, Boone et al., 2016, Di Gregorio et al., 2016, Hergovich, 2016, Poon et al., 2016, Suijkerbuijk et al., 2016, Yadav et al., 2016, Buchon and Osman, 2015, Copf, 2015, Enomoto et al., 2015, Hariharan, 2015, Irvine and Harvey, 2015, Pasco et al., 2015, Thompson, 2015, Thompson and Sahai, 2015, Amoyel and Bach, 2014, Sadeqzadeh et al., 2014, Degoutin et al., 2013, Deng et al., 2013, Huang et al., 2013, Konsavage and Yochum, 2013, Kwon et al., 2013, Lucas et al., 2013, Pastor-Pareja and Xu, 2013, Pomerantz and Blau, 2013, Puram and Bonni, 2013, Takashima et al., 2013, Vilmos et al., 2013, Wu and Wu, 2013, Yang and Hata, 2013, Hergovich and Hemmings, 2012, Jin et al., 2012, Bao et al., 2011, Finan et al., 2011, Halder and Johnson, 2011, Reddy and Irvine, 2011, Baumgartner et al., 2010, Das Thakur et al., 2010, Genevet et al., 2010, Grzeschik et al., 2010, Ho et al., 2010, Ren et al., 2010, Ribeiro et al., 2010, Yu et al., 2010, Classen et al., 2009, Genevet et al., 2009, Hamaratoglu et al., 2009, Oh et al., 2009, Deng et al., 2008, Dutta and Baehrecke, 2008, Goulev et al., 2008, Mattila et al., 2008, Nicolay and Frolov, 2008, Oh and Irvine, 2008, Yu et al., 2008, Dong et al., 2007, Feng and Irvine, 2007, Hamaratoglu et al., 2007, Jukam and Desplan, 2007, Martin et al., 2007, Polesello and Tapon, 2007, Willecke et al., 2007, Colombani et al., 2006, Emoto et al., 2006, Polesello et al., 2006, Silva, 2006, Silva et al., 2006, Willecke et al., 2006, Wheeler et al., 2004)

Hippo kinase

(Jukam et al., 2013)

MST

hippo

(Agrawal et al., 2021, Vigneswaran et al., 2021, Otsuki and Brand, 2020, Brown et al., 2019, Jia et al., 2018, Hevia et al., 2017, Atwell et al., 2016, Losick et al., 2016, Ng et al., 2016, Di Cara et al., 2015, Kwon et al., 2015, Johnston, 2014, Jones and Srivastava, 2014, Harvey et al., 2013, Perea et al., 2013, Wehr et al., 2013, Justiniano et al., 2012, Nagaraj et al., 2012, Verghese et al., 2012, Chen et al., 2011, Chen et al., 2010, Fang and Adler, 2010, Menéndez et al., 2010, Milton et al., 2010, Zecca and Struhl, 2010, Badouel et al., 2009, Cruz et al., 2009, Zhang et al., 2009, Bakal et al., 2008, Rafel and Milán, 2008, Sun et al., 2008, Meignin et al., 2007, Menut et al., 2007, Tyler and Baker, 2007, Cho et al., 2006, Hariharan, 2006, Herz et al., 2006, Nolo et al., 2006, Bateman and McNeill, 2005, Formstecher et al., 2005, Wang and Montell, 2005, Hafen and Stocker, 2003, Wu et al., 2003)

Secondary FlyBase IDs

FBgn0034453
FBgn0069363

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

Hippo_Pathway_interaction_map

interactome

Interaction map generated by purification of Hippo pathway factors, with identification of copurifying proteins by mass spectrometry.

Study result (0)

Result

Result Type

Title

References (519)

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