FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Slmap, hpo
FlyBase ID
FBig0000027995
Interaction Network
Interactions Browser links
Slmap network
hpo network
Reported Interactions
FBrf0218395-8327.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Slmap 
unspecified role
Slmap 
unspecified role
Slmap 
unspecified role
hpo 
unspecified role
hpo 
unspecified role
hpo 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 61.69759

FBrf0237510-2.coIP.WB
Reference
Zheng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Slmap 

Slmap

bait

HA or FLAG tag

Slmap 
hpo 

Hpo

prey

Myc tag

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
kinase domain
mutation disrupting interaction
aa 71

K71R, kinase dead

Slmap 
N-terminal domain
sufficient binding region
aa 1-343

coordinates relative to AA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zheng et al., 2017)
FBrf0237510-3.coIP.WB
Reference
Zheng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

Myc or FLAG tag

hpo 
hpo 
Slmap 

Slmap

prey

HA tag

Slmap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Slmap 
FHA domain residues 260,261,262
mutation disrupting interaction
aa 260,261,262

R260A,N261A,H262A

hpo 
linker domain
mutation disrupting interaction
aa 356,374,387,421,431

T356A,T374A,T387A,T421A,T431A

hpo 
linker domain residue 356
mutation disrupting interaction
aa 356

T356A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zheng et al., 2017)
FBrf0237510-4.PD.WB
Reference
Zheng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Slmap 

Slmap

bait

GST tag

Slmap 
hpo 

Hpo

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Slmap 
FHA domain
sufficient binding region
aa 205-343
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.

(Zheng et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)