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General Information
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| Term |
tandem affinity purification |
ID (Ontology) |
MI:0676 (Molecular Interactions) |
| Definition |
Tandem affinity purification allows rapid purification under native conditions of complexes, even when expressed at their natural level. Prior knowledge of complex composition or function is not required. The TAP method requires fusion of the a multiple tag, either N- or C-terminally, to the target (or bait) protein of interest. The multiple tag allows two steps purification steps ensuring a highly selective complex purification.[ PubMed:11403571 ] |
| Also Known As |
"TAP" ; "tap" |
| Comment |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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tandem affinity purification (all annotations which use CV term, excluding "NOT" statements) | 144 |
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Relationships