FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

tandem affinity purification

ID (Ontology)

MI:0676 (Molecular Interactions)

Definition

Tandem affinity purification allows rapid purification under native conditions of complexes, even when expressed at their natural level. Prior knowledge of complex composition or function is not required. The TAP method requires fusion of the a multiple tag, either N- or C-terminally, to the target (or bait) protein of interest. The multiple tag allows two steps purification steps ensuring a highly selective complex purification.[ PubMed:11403571 ]

Also Known As

"TAP" ; "tap"

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
tandem affinity purification (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

chromatography technology__
affinity technology________|
                           affinity chromatography technology
                            |__tandem affinity purification  144 rec.

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	affinity chromatography technology
Part of
Synonyms & Secondary IDs
Synonyms
	"TAP" EXACT PSI-MI-alternate "tap" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts