FlyBase Gene Report: Dmel\Slik

General Information

Symbol

Dmel\Slik

Species

D. melanogaster

Name

Sterile20-like kinase

Annotation Symbol

CG4527

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035001

Gene Model Status

Current

Stock Availability

20 publicly available

Enzyme Name (EC)

Gene Summary

Sterile20-like kinase (Slik) encodes a member of the Sterile-20 kinase family. It acts via Raf to promote cell proliferation in imaginal disc epithelia. Independently, Slik acts via the product of Moe to support epithelial integrity. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Plkk1

Key Links

Genomic Location

Cytogenetic map

60C7-60C8

Sequence location

2R:24,392,129..24,402,680 [-]

Recombination map

2-106

RefSeq locus

NT_033778 REGION:24392129..24402680

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein serine/threonine kinase activity

inferred from mutant phenotype

(Carreno et al., 2008)

inferred from direct assay

(Hughes and Fehon, 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN004698611

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR022165

(InterPro Project Members, 2004-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in branching involved in open tracheal system development

inferred from mutant phenotype

(Ukken et al., 2014)

involved_in cell morphogenesis

inferred from mutant phenotype

(Carreno et al., 2008)

involved_in cortical actin cytoskeleton organization

inferred from mutant phenotype

(Carreno et al., 2008)

involved_in establishment of mitotic spindle orientation

inferred from mutant phenotype

(Carreno et al., 2008)

involved_in metaphase/anaphase transition of mitotic cell cycle

inferred from mutant phenotype

(Carreno et al., 2008)

involved_in mitotic spindle organization

inferred from mutant phenotype

(Carreno et al., 2008)

involved_in positive regulation of cell population proliferation

inferred from mutant phenotype

(Hipfner and Cohen, 2003)

involved_in rhabdomere development

inferred from mutant phenotype

(Ogi et al., 2019)

involved_in rhabdomere membrane biogenesis

inferred from mutant phenotype

(Ogi et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN004698611

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Carreno et al., 2008)

located_in cleavage furrow

inferred from direct assay

(Carreno et al., 2008)

located_in mitotic spindle midzone

inferred from direct assay

(Carreno et al., 2008)

located_in rhabdomere

inferred from direct assay

(Ogi et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN004698611

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

GERMINAL CENTER KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

GERMINAL CENTER KINASES -: Germinal center kinases (GCKs) are serine/threonine kinases characterised by an N-terminal STE20 kinase domain. They have diverse roles in signaling pathways; some are MAPK kinase kinase kinases, activating MAPK kinase kinases. (Adapted from FBrf0225276 and FBrf0228279).

Summary (Interactive Fly)

links actin and microtubule filaments at the plasma membrane to regulate cell morphogenesis - crucial for proper talin recruitment and maintenance of muscle attachment - phosphorylates and activates Moesin in developing epithelial tissues - p-Moesin asymmetric distribution is determined by components of the apical polarity complex and Slik kinase - Slik promotes epithelial cell proliferation and tissue growth - Slik is necessary for the activation of Moesin at the tracheal luminal membrane - Slik co-regulates Merlin or Moesin activity whereby phosphorylation inactivates Merlin, but activates Moesin

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Slik for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W179)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Slik-RA

FBtr0072363

NM_138064

4863

1300

Slik-RB

FBtr0072364

NM_166669

6419

1703

Slik-RD

FBtr0072366

NM_206213

6812

1703

Slik-RE

FBtr0100470

NM_001014549

4642

1342

Slik-RF

FBtr0290019

NM_001144280

5818

1635

Slik-RG

FBtr0332925

NM_206214

5405

1365

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Slik-PA

FBpp0072270

147.5

1300

6.54

FBpp0072271

192.1

1703

6.60

FBpp0072273

192.1

1703

6.60

FBpp0099900

152.1

1342

6.39

FBpp0288458

184.6

1635

6.72

FBpp0305145

155.1

1365

6.63

A4UZU8

NP_995936

AAS64766

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1703 aa isoforms: Slik-PB, Slik-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Slik using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.45

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Dorus et al., 2006)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Carreno et al., 2008)

located_in cleavage furrow

inferred from direct assay

(Carreno et al., 2008)

located_in mitotic spindle midzone

inferred from direct assay

(Carreno et al., 2008)

located_in rhabdomere

inferred from direct assay

(Ogi et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Slik in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Slik

View exonic expression by tissue for Dmel\Slik

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035001

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37893/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035001

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035001

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035001

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4527

Flygut - An atlas of the Drosophila adult midgut

FBgn0035001

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Slik

Transgenic constructs containing regulatory region of Slik

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6278

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^ptc.PU

Slik^UAS.cPa

lethal - all die before end of larval stage, with Scer\GAL4^Mef2.PU, Slik¹

Slik^UAS.cUa

lethal - all die before end of larval stage, with Scer\GAL4^Mef2.PU, Slik^UAS.cUa

Slik¹

lethal - all die before end of larval stage | partially | recessive

Slik^KG04837

lethal - all die before end of pupal stage | recessive

Slik¹

lethal - all die during embryonic stage

Slik¹

partially lethal, with Scer\GAL4^ptc.PU

Slik^{T186A.T192A.UAS}

partially lethal - majority die (with Slik¹)

Slik^KG04837

partially lethal - majority die (with Slik^KG04837)

Slik¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Slik^GD11338

viable, with Scer\GAL4^Mef2.PR

Slik^GD11338

viable, with Scer\GAL4^pnr-MD237

Slik^GD11338

viable, with Scer\GAL4^tin.CΔ4

Slik^GD11338

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Slik^GL00051

Other Phenotypes

Allele

abnormal cell adhesion | embryonic stage 17

Slik¹

abnormal cell adhesion | embryonic stage 17 (with Df(2R)BSC603)

Slik¹

abnormal developmental rate

Slik¹

abnormal developmental rate | recessive

Slik^KG04837

abnormal larval locomotory behavior, with Scer\GAL4^elav.PLu

Slik^HMC03770

abnormal mitotic cell cycle | cell non-autonomous, with Scer\GAL4^ptc-559.1

Slik^UAS.cHa

abnormal neuroanatomy | adult stage

Slik¹

abnormal neuroanatomy | pupal stage | somatic clone

Slik¹

abnormal size | adult stage | somatic clone

Slik¹

abnormal size | larval stage, with Scer\GAL4^elav.PLu

Slik^HMC03770

decreased body size, with Scer\GAL4^arm.PS, Slik¹

Slik^UAS.cHa

decreased body size, with Scer\GAL4^arm.PS, Slik^UAS.cHa

Slik¹

decreased body size | larval stage

Slik^KG04837

decreased cell number | larval stage | somatic clone

Slik¹

decreased rate of adult locomotory behavior, with Scer\GAL4^elav.PLu

Slik^HMC03770

hyperplasia | adult stage, with Scer\GAL4^ptc-559.1

Slik^UAS.cHa

increased cell death (with Slik¹)

Slik^KG04837

increased cell death (with Slik^KG04837)

Slik¹

increased cell death | larval stage | somatic clone

Slik¹

increased cell death | somatic clone

Slik¹

increased cell death | somatic clone (with Slik¹)

Slik^KG04837

increased cell death | somatic clone (with Slik^KG04837)

Slik¹

increased cell number, with Scer\GAL4^ptc-559.1

Slik^UAS.cHa

increased cell number | cell non-autonomous, with Scer\GAL4^ptc-559.1

Slik^UAS.cHa

increased occurrence of cell division | cell non-autonomous | third instar larval stage, with Scer\GAL4^ap.PU

Slik^UAS.cPa

increased occurrence of cell division | cell non-autonomous | third instar larval stage, with Scer\GAL4^ptc.PU

increased occurrence of cell division | larval stage, with Scer\GAL4^ptc-559.1

Slik^UAS.cHa

short lived, with Scer\GAL4^elav.PLu

Slik^HMC03770

some die during embryonic stage, with Scer\GAL4^Mef2.PU, Slik¹

Slik^UAS.cUa

some die during embryonic stage, with Scer\GAL4^Mef2.PU, Slik^UAS.cUa

Slik¹

some die during larval stage | recessive

Slik^KG04837

some die during pupal stage | recessive

Slik¹

Phenotype manifest in

Allele

embryonic somatic muscle cell | embryonic stage 17

Slik¹

embryonic somatic muscle cell | embryonic stage 17 (with Df(2R)BSC603)

Slik¹

eye photoreceptor cell stalk | adult stage

Slik¹

eye photoreceptor cell stalk | pupal stage | somatic clone

Slik¹

first posterior wing cell, with Scer\GAL4^arm.PS

Slik^UAS.cHa

larval ventral nerve cord, with Scer\GAL4^elav.PLu

Slik^HMC03770

NMJ bouton | larval stage, with Scer\GAL4^elav.PLu

Slik^HMC03770

ommatidium & microvillus | somatic clone

Slik¹

outer photoreceptor cell | pupal stage | somatic clone

Slik¹

peripodial epithelium, with Scer\GAL4^ptc-559.1

Slik^UAS.cHa

peripodial epithelium | cell non-autonomous | third instar larval stage, with Scer\GAL4^ap.PU

Slik^UAS.cPa

peripodial epithelium | cell non-autonomous | third instar larval stage, with Scer\GAL4^ptc.PU

peripodial epithelium | third instar larval stage, with Scer\GAL4^ptc.PU

Slik^{T186A.T192A.UAS}

rhabdomere | somatic clone

Slik¹

rhabdomere microvillus | adult stage

Slik¹

rhabdomere of eye photoreceptor cell | adult stage

Slik¹

rhabdomere of eye photoreceptor cell | pupal stage | somatic clone

Slik¹

wing (with Slik¹)

Slik^KG04837

wing (with Slik^KG04837)

Slik¹

wing & epithelial cell

wing & epithelial cell | ectopic

wing | somatic clone (with Slik¹)

Slik^KG04837

wing | somatic clone (with Slik^KG04837)

Slik¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (80)

Hsap\STK10

12 of 14

Yes

Hsap\SLK

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC407835

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (47)

Rnor\Slk

12 of 14

Yes

Rnor\Stk10

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Map3k12

1 of 14

Rnor\Map3k13

1 of 14

Rnor\Map3k14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (52)

Mmus\Slk

12 of 14

Yes

Mmus\Stk10

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (38)

Xtro\stk10

9 of 13

Yes

Xtro\slk

6 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\map3k2

1 of 13

Xtro\map3k8

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (58)

Drer\slka

11 of 14

Yes

Drer\stk10

11 of 14

Yes

Drer\slkb

10 of 14

Yes

Drer\si:dkey-81j8.6

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-151g10.3

1 of 14

Drer\si:zfos-2326c3.2

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (32)

Cele\gck-4

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (25)

Agam\AgaP_AGAP010551

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (57)

Atha\AT3G15220

2 of 13

Yes

Atha\ATMAP4K ALPHA1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\MAPKKK20

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (19)

Scer\BCK1

1 of 13

Yes

Scer\CDC15

1 of 13

Yes

Scer\CLA4

1 of 13

Yes

Scer\GIN4

1 of 13

Yes

Scer\KCC4

1 of 13

Yes

Scer\KIC1

1 of 13

Yes

Scer\MKK1

1 of 13

Yes

Scer\MKK2

1 of 13

Yes

Scer\PBS2

1 of 13

Yes

Scer\PKC1

1 of 13

Yes

Scer\SKM1

1 of 13

Yes

Scer\SPS1

1 of 13

Yes

Scer\SSK2

1 of 13

Yes

Scer\SSK22

1 of 13

Yes

Scer\STE7

1 of 13

Yes

Scer\STE11

1 of 13

Yes

Scer\STE20

1 of 13

Yes

Scer\SWE1

1 of 13

Yes

Scer\YPK1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (10)

Spom\cdc7

1 of 12

Yes

Spom\gad8

1 of 12

Yes

Spom\mik1

1 of 12

Yes

Spom\nak1

1 of 12

Yes

Spom\pka1

1 of 12

Yes

Spom\ppk11

1 of 12

Yes

Spom\sid1

1 of 12

Yes

Spom\sid2

1 of 12

Yes

Spom\wee1

1 of 12

Yes

Spom\wis4

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Slik. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (25)

fray

3 of 13

GckIII

3 of 13

hppy

3 of 13

hpo

2 of 13

mbt

2 of 13

msn

2 of 13

Pak

2 of 13

Stlk

2 of 13

Tao

2 of 13

Aduk

1 of 13

Akt

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

gek

1 of 13

hep

1 of 13

lic

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

PKD

1 of 13

Sik3

1 of 13

Wee1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

neurodevelopmental disorder, SLK-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Slik^GD11338

ameliorates cancer

modeled by Pten^KK109278

(Parniewska and Stocker, 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

STK10; serine/threonine kinase 10

12 of 14

603919

SLK; STE20 like kinase

11 of 14

STE20-LIKE PROTEIN KINASE; SLK

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

Slik

Hsap\SLK

(Alabdi et al., 2025)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Slik

enzymatic study, autoradiography

(Panneton et al., 2015)

Slik - CG10939

anti tag coimmunoprecipitation, anti tag western blot

(Hughes et al., 2010)

Slik - Cka

anti tag coimmunoprecipitation, anti tag western blot

(De Jamblinne et al., 2020)

Slik - Mer

pull down, autoradiography

(Hughes and Fehon, 2006)

Slik - Mocs2B

anti tag coimmunoprecipitation, peptide massfingerprinting

(Suganuma et al., 2010)

Slik - Moe

pull down, autoradiography, enzymatic study

(De Jamblinne et al., 2020, Panneton et al., 2015, Hughes and Fehon, 2006)

Slik - Raf

anti tag coimmunoprecipitation, anti tag western blot

(Hipfner and Cohen, 2003)

Slik - Rho1

pull down, western blot, anti bait coimmunoprecipitation, bimolecular fluorescence complementation, luminiscence technology, anti tag coimmunoprecipitation, anti tag western blot

(Bagci et al., 2020)

Slik - Strip

anti tag coimmunoprecipitation, anti tag western blot

(De Jamblinne et al., 2020)

Slik - aru

experimental knowledge based

(Guruharsha et al., 2011)

Slik - gcm

two hybrid

(Jacques et al., 2009)

Slik - rhea

protein kinase assay, western blot

(Katzemich et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Slik

suppressible

hep

(Hipfner and Cohen, 2003)

Slik

suppressible

Moe

(Hipfner et al., 2004)

Slik

enhanceable

Raf

(Hipfner and Cohen, 2003)

Slik

suppressible

Raf

(Hipfner and Cohen, 2003)

Slik

suppressible

rhea

(Katzemich et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Mer

enhanceable

Slik

(Hughes and Fehon, 2006)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0035001

MIST (genetic) - An integrated Molecular Interaction Database

37893

MIST (protein-protein) - An integrated Molecular Interaction Database

37893

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

B7YZQ1

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-106

Cytogenetic map

60C7-60C8

Sequence location

2R:24,392,129..24,402,680 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60C7-60C8

Limits computationally determined from genome sequence between P{PZ}bs⁰³²⁶⁷&P{lacW}l(2)k10502^k10502 and P{lacW}Nurf-38^k16102

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-106

(FlyBase, 2016)

2-110.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

600520

w^*; P{UAS-Slik.H}29A

600521

P{UAS-Slik.H}17B, w^*

43349

w^*; P{GSV1}Rpn8^C125 Slik^C125

602805

y¹ v¹; P{CFD4FLPOUT-msn-Slik}attP40/CyO

14435

y¹; P{SUPor-P}Slik^KG04837/CyO; ry⁵⁰⁶

Kyoto

123806

w¹¹¹⁸; P{RS3}Slik^CB-5766-3

126265

w¹¹¹⁸; P{RS5}Slik^5-SZ-3976

4527R-2

4527R-3

v43783

w¹¹¹⁸; P{GD11338}v43783

v43784

w¹¹¹⁸; P{GD11338}v43784

v341140

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00161}attP40/CyO-GFP

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (58)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI24227

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035001

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035001

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Alves et al., 2006)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

S2R+-Slik-KO-22C2

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : S2 cells expressing GFP tagged Slik, or fragments of Slik, under the control of Mt promoter.
(De Jamblinne et al., 2020)

Other Comments

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Source for identity of Plkk1 CG4527 was sequence comparison ( date:030717 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Plkk1 CG4527

(FlyBase, 1996-)

Source for identity of: Slik slik

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG4527

(National Institute of Genetics Fly Stocks, 2022-, Christis and Munro, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Molnar et al., 2006, Chen et al., 2004, Orian et al., 2003, Boutanaev et al., 2002, Dan et al., 2001)

DPlkk1

(Tavares, 2003.7.17)

PLKK1

(Carreno et al., 2008)

Plkk/Slik

(Roubinet et al., 2007)

Plkk1

(Kunda et al., 2008, Dorus et al., 2006, Dorus et al., 2006, Molnar et al., 2006, Foley and O'Farrell, 2004)

SLIK

(Delpire, 2009)

SLK

(Morrison et al., 2000)

Slik1

(Jacques et al., 2009)

Slk

(Garcia et al., 2007)

dPlkk

(Alves et al., 2006)

dPlkk/Slik

(Carreno et al., 2007)

slik

(Parniewska and Stocker, 2020, Xu et al., 2019, Levinson and Cagan, 2016, Sopko et al., 2014, Tan et al., 2014, de Celis et al., 2013.9.11, Japanese National Institute of Genetics, 2012.5.21, Hughes et al., 2010, Suganuma et al., 2010, Parrish et al., 2009, Junion et al., 2007, Alves et al., 2006, Hughes and Fehon, 2006, Hipfner and Cohen, 2003)

slk

(Hipfner et al., 2002)

Name Synonyms

Polo kinase kinase 1

(Tavares, 2003.7.17)

Slik

(Li et al., 2023)

Ste20 kinase

(Hughes and Fehon, 2006)

Sterile-20

(Ukken et al., 2014)

Sterile20-like kinase

(Hipfner et al., 2002)

dPlkk/Slik threonine kinase

(Carreno et al., 2007)

polo kinase kinase 1

(Echard et al., 2004)

sterile 20-like kinase

(Taniguchi et al., 2014)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 82 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37893

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W179

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W179

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035001

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37893/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035001

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035001

FlyMine - An integrated database for Drosophila genomics

FBgn0035001

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4527

MARRVEL_MODEL - MARRVEL (model organism gene)

37893

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035001

DroID - A comprehensive database of gene and protein interactions.

FBgn0035001

DRSC - Results frm RNAi screens

FBgn0035001

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035001

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035001

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4527

Flygut - An atlas of the Drosophila adult midgut

FBgn0035001

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0035001

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000382

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

37893

MIST (protein-protein) - An integrated Molecular Interaction Database

37893

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

B7YZQ1

References (114)

The following external sites may use different assemblies or annotations than FlyBase.

-0.45

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