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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108531
Interaction Network
Interactions Browser links
gcm network
Slik network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0208351-12.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gcm 

gcm

bait

fused to LexA DNA-binding domain

gcm 
gcm 
Slik 

Slik 1

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gcm 
N-terminal region
sufficient binding region
aa 1-261

The N-terminal construct of gcm used as bait includes the DBD, the NLS and the PEST domain.

gcm 
NLS and PEST domain
necessary binding region
aa 187-261
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/VP16-AD

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)