FlyBase Gene Report: Dmel\Pak3

General Information

Symbol

Dmel\Pak3

Species

D. melanogaster

Name

Pak3

Annotation Symbol

CG14895

Feature Type

protein_coding_gene

FlyBase ID

FBgn0044826

Gene Model Status

Current

Stock Availability

25 publicly available

Gene Summary

Pak3 (Pak3) encodes a Ser/Thr kinase implicated in myoblast fusion, synapse development, wound healing and border cell migration. It functions downstream of the small Rac GTPases to regulate actin cytoskeletal organization. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dpak3, DmPAK3

Key Links

Genomic Location

Cytogenetic map

89B16-89B17

Sequence location

3R:16,445,007..16,453,821 [-]

Recombination map

3-58

RefSeq locus

NT_033777 REGION:16445007..16453821

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein serine/threonine kinase activity

inferred from direct assay

(Mentzel and Raabe, 2005)

enables small GTPase binding

inferred from physical interaction with FLYBASE:Rac1; FB:FBgn0010333

(Mentzel and Raabe, 2005)

inferred from physical interaction with FLYBASE:Rac2; FB:FBgn0014011

(Mentzel and Raabe, 2005)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000684825

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR033923

(InterPro Project Members, 2004-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333

(Mentzel and Raabe, 2005)

involved_in border follicle cell migration

inferred from mutant phenotype

(Zhou et al., 2022)

involved_in cell projection organization

inferred from mutant phenotype

(Zhou et al., 2022)

involved_in myoblast fusion

inferred from mutant phenotype

(Duan et al., 2012)

involved_in positive regulation of synapse assembly

inferred from genetic interaction with FLYBASE:spas; FB:FBgn0039141

(Ozdowski et al., 2011)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in cell migration

inferred from biological aspect of ancestor with PANTHER:PTN001221195

(GO Reference Genome Project, 2011-)

involved_in cellular response to starvation

inferred from biological aspect of ancestor with PANTHER:PTN000685129

(GO Reference Genome Project, 2011-)

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN001969365

(GO Reference Genome Project, 2011-)

involved_in regulation of axonogenesis

inferred from biological aspect of ancestor with PANTHER:PTN000685295

(GO Reference Genome Project, 2011-)

involved_in regulation of MAPK cascade

inferred from biological aspect of ancestor with PANTHER:PTN001969365

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Mentzel and Raabe, 2005)

located_in ruffle

inferred from direct assay

(Mentzel and Raabe, 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000684825

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

P21-ACTIVATED KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

P21-ACTIVATED KINASES -: PAK proteins are serine/threonine kinases characterised by an C-terminal STE20 kinase domain and an N-terminal p21-binding domain which binds RhoGTPases. PAKs are autoinhibited by homodimerization. PAKs are involved in the regulation of cytoskeleton organisation and other signaling cascades. (Adapted from FBrf0187582).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pak3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4KGS4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Stop-codon suppression (UAG) postulated; FBrf0216884.

Gene model reviewed during 5.44

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pak3-RA

FBtr0083312

NM_142288

3244

569

Pak3-RC

FBtr0305003

NM_169719

4983

569

Pak3-RD

FBtr0330047

NM_001275709

4983

583

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pak3-PA

FBpp0082763

64.1

569

6.00

FBpp0293540

64.1

569

6.00

FBpp0303080

65.7

583

6.34

A0A0B4KGS4

NP_001262638

AGB96018

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

569 aa isoforms: Pak3-PA, Pak3-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pak3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pak3_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.89

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13

embryonic dorsal epidermis

(Bahri et al., 2010)

embryonic stage 14

fusion competent cell

(Duan et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Mentzel and Raabe, 2005)

located_in ruffle

inferred from direct assay

(Mentzel and Raabe, 2005)

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}Pak3^DRH-5

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

antennal disc

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval lymph gland

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval salivary gland

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pak3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pak3

View exonic expression by tissue for Dmel\Pak3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41995/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0044826

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0044826

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0044826

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14895

Flygut - An atlas of the Drosophila adult midgut

FBgn0044826

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 42 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pak3

Transgenic constructs containing regulatory region of Pak3

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)Exel6269

(Ryder, 2004.4.26)

Df(3R)Exel7330

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(3R)RS6279.exc44

(Thanawala et al., 2013)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Pak3^LA00012

lethal - all die before end of first instar larval stage | recessive

Pak3^Df

lethal - all die before end of larval stage (with Pak3^d02472)

Pak3^Df

lethal - all die before end of larval stage (with Pak3^Df)

Pak3^d02472

partially lethal - majority die

Pak3^d02472

partially lethal - majority die (with Pak3^Df)

Pak3^e00329

partially lethal - majority die (with Pak3^e00329)

Pak3^Df

partially lethal - majority die | adult stage, with Scer\GAL4^pnr-MD237

Pak3^GD8481

partially lethal - majority live

Pak3^e00329

partially lethal - majority live (with Pak3^d02472)

Pak3^e00329

partially lethal - majority live (with Pak3^e00329)

Pak3^d02472

viable

Pak3^CB04117

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pak3^GD8481

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Pak3^GL00287

Other Phenotypes

Allele

abnormal neuroanatomy, with Scer\GAL4²²¹

Pak3^UAS.GFP

abnormal neuroanatomy, with Scer\GAL4^elav.PU

Pak3^LA00012

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

abnormal neurophysiology (with Pak3^d02472)

Pak3^e00329

abnormal neurophysiology (with Pak3^e00329)

Pak3^d02472

flightless, with Scer\GAL4^Mef2.PR

Pak3^GD8481

visible, with Scer\GAL4^Mef2.PR

Pak3^GD8481

visible, with Scer\GAL4^pnr-MD237

Pak3^GD8481

Phenotype manifest in

Allele

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

abdominal dorsal multidendritic neuron ddaD | third instar larval stage, with Scer\GAL4²²¹

Pak3^UAS.GFP

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

Pak3^UAS.GFP

dendritic tree | third instar larval stage, with Scer\GAL4²²¹

Pak3^UAS.GFP

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

embryonic/larval cuticle

embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PU

Pak3^LA00012

embryonic myoblast

Pak3^del

embryonic myoblast, with Pak3^del, Scer\GAL4^twi.PG

Pak3^{K322A.UAS.C.Tag:V5}

embryonic myoblast, with Pak3^{K322A.UAS.C.Tag:V5}, Scer\GAL4^twi.PG

Pak3^del

embryonic myoblast, with Scer\GAL4^twi.PG

Pak3^{K322A.UAS.C.Tag:V5}

filamentous actin

Pak3^del

microtubule, with Scer\GAL4^elav.PU

Pak3^LA00012

NMJ bouton, with Scer\GAL4^elav.PU

Pak3^LA00012

trichogen cell, with Scer\GAL4^pnr-MD237

Pak3^GD8481

wing, with Scer\GAL4^Mef2.PR

Pak3^GD8481

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (79)

Hsap\PAK1

6 of 14

Yes

Hsap\PAK2

6 of 14

Yes

Hsap\PAK3

6 of 14

Yes

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC407835

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Pak1

6 of 14

Yes

Rnor\Pak2

6 of 14

Yes

Rnor\Pak3

6 of 14

Yes

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (37)

Mmus\Pak1

6 of 14

Yes

Mmus\Pak2

6 of 14

Yes

Mmus\Pak3

6 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (36)

Xtro\pak2

4 of 13

Yes

Xtro\pak3

4 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (45)

Drer\pak2a

6 of 14

Yes

5 of 14

5 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:zfos-2326c3.2

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (28)

Cele\max-2

5 of 14

Yes

Cele\pak-1

5 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (32)

Agam\AgaP_AGAP001790

12 of 12

Yes

3 of 12

3 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (103)

Atha\MAP3KA

2 of 13

Yes

Atha\MAPKKK5

2 of 13

Yes

Atha\SIK1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G23310

1 of 13

Atha\AT3G45670

1 of 13

Yes

Atha\AT3G45790

1 of 13

Yes

Atha\AT3G46140

1 of 13

Yes

Atha\AT3G46160

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G49470

1 of 13

Atha\AT5G55560

1 of 13

Atha\AT5G57035

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\MAPKKK14

1 of 13

Yes

Atha\MAPKKK15

1 of 13

Yes

Atha\MAPKKK16

1 of 13

Yes

Atha\MAPKKK17

1 of 13

Yes

Atha\MAPKKK18

1 of 13

Yes

Atha\MAPKKK19

1 of 13

Yes

Atha\MAPKKK20

1 of 13

Yes

Atha\MAPKKK21

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (16)

Scer\SKM1

6 of 13

Yes

Scer\CLA4

5 of 13

Yes

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\shk2

6 of 12

Yes

Spom\shk1

5 of 12

Spom\ppk2

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pak3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (29)

Pak

6 of 13

mbt

5 of 13

hpo

2 of 13

msn

2 of 13

Tao

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

for

1 of 13

fray

1 of 13

GckIII

1 of 13

hppy

1 of 13

lic

1 of 13

Lk6

1 of 13

lok

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Tlk

1 of 13

wnd

1 of 13

Wnk

1 of 13

wts

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PAK3; p21 (RAC1) activated kinase 3

model of non-syndromic X-linked intellectual disability 30

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 8 )

Allele

Disease

Interaction

References

Pak3^LA00012

exacerbates hereditary spastic paraplegia 4

modeled by spas^T32

(Ozdowski et al., 2011)

Pak3^d02472

ameliorates hereditary spastic paraplegia

modeled by spas^5.75

(Ozdowski et al., 2011)

ameliorates hereditary spastic paraplegia 4

modeled by spas^T32

(Ozdowski et al., 2011)

modeled by spas^5.75

(Ozdowski et al., 2020)

Pak3^GL00287

ameliorates hereditary spastic paraplegia 4

modeled by spas^5.75

(Ozdowski et al., 2020)

Pak3^GD8481

ameliorates hereditary spastic paraplegia 4

modeled by spas^5.75

(Ozdowski et al., 2020)

Pak3^KK111386

ameliorates spinocerebellar ataxia type 1

modeled by Hsap\ATXN1^82Q.UAS

(Bondar et al., 2018)

Pak3^NIG.14895R

ameliorates hereditary spastic paraplegia 4

modeled by spas^5.75

(Ozdowski et al., 2020)

Pak3^Df

ameliorates hereditary spastic paraplegia

modeled by spas^5.75

(Ozdowski et al., 2011)

ameliorates hereditary spastic paraplegia 4

modeled by spas^T32

(Ozdowski et al., 2011)

modeled by spas^5.75

(Ozdowski et al., 2020)

Pak3^e00329

ameliorates hereditary spastic paraplegia 4

modeled by spas^T32

(Ozdowski et al., 2011)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PAK1; p21 (RAC1) activated kinase 1

6 of 14

602590

INTELLECTUAL DEVELOPMENTAL DISORDER WITH MACROCEPHALY, SEIZURES, AND SPEECH DELAY; IDDMSSD

PAK2; p21 (RAC1) activated kinase 2

6 of 14

605022

KNOBLOCH SYNDROME 2; KNO2

PAK3; p21 (RAC1) activated kinase 3

6 of 14

300142

INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED 30; XLID30

non-syndromic X-linked intellectual disability 30

BUB1B-PAK6; BUB1B-PAK6 readthrough

3 of 14

PAK4; p21 (RAC1) activated kinase 4

3 of 14

605451

PAK5; p21 (RAC1) activated kinase 5

3 of 14

608038

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pak3

enzymatic study, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Mentzel et al., 2009, Mentzel and Raabe, 2005)

Pak3 - Cdc42

anti tag coimmunoprecipitation, western blot

(Mentzel and Raabe, 2005)

Pak3 - CkIIβ

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Mentzel et al., 2009)

Pak3 - CkIIβ2

anti tag coimmunoprecipitation, anti tag western blot

(Mentzel et al., 2009)

Pak3 - Rac1

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Duan et al., 2012, Mentzel and Raabe, 2005)

Pak3 - Rac2

anti tag coimmunoprecipitation, western blot

(Mentzel and Raabe, 2005)

Pak3 - Ssl

anti tag coimmunoprecipitation, anti tag western blot

(Mentzel et al., 2009)

Pak3 - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pak3 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pak3 - scrib

anti tag coimmunoprecipitation, western blot

(Cervantes-Sandoval et al., 2016, Bahri et al., 2010)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pak3

enhanceable

Pak

(Duan et al., 2012, Bahri et al., 2010)

Pak3

suppressible

Pak

(Duan et al., 2012)

Pak3

enhanceable

SCAR

(Duan et al., 2012)

Pak3

enhanceable

WASp

(Duan et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

Pak3

(Hattori et al., 2013)

Pak

enhanceable

Pak3

(Bahri et al., 2010)

SCAR

enhanceable

Pak3

(Duan et al., 2012)

spas

suppressible

Pak3

(Ozdowski et al., 2011)

spas

enhanceable

Pak3

(Ozdowski et al., 2011)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67040

DroID - A comprehensive database of gene and protein interactions.

FBgn0044826

MIST (genetic) - An integrated Molecular Interaction Database

41995

MIST (protein-protein) - An integrated Molecular Interaction Database

41995

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Hippo signaling pathway - multiple species

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8IGZ0

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-58

Cytogenetic map

89B16-89B17

Sequence location

3R:16,445,007..16,453,821 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89B16-89B17

Limits computationally determined from genome sequence between P{PZ}gish⁰⁴⁸⁹⁵&P{EP}EP3171 and P{lacW}CSN5^L4032

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-58

(FlyBase, 2016)

3-58.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (25)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

52302

w^*; P{UASp-mChFP-Pak3}30

52303

w^*; P{sqh-Pak3.RBD-GFP}20

52304

w^*; P{sqh-Pak3.RBD-GFP}30

42664

y¹ v¹; P{TRiP.HMS02500}attP40

Kyoto

123187

w¹¹¹⁸; P{RS3}Pak3^CB-0469-3

123594

w¹¹¹⁸; P{RS3}Pak3^CB-5374-3

123909

w¹¹¹⁸; P{RS3}Pak3^CB-6089-3

NIG-Fly

14895R-1

P{NIG.14895R}1

14895R-2

P{NIG.14895R}2

HMS02500

y¹ v¹; P{TRiP.HMS02500}attP40/CyO

VDRC

v107260

P{KK111386}VIE-260B

v39844

w¹¹¹⁸; P{GD8481}v39844

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (95)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0044826

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0044826

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Duan et al., 2012, Bahri et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: increased or polarized (uneven) accumulation of F-actin.

(Kiger et al., 2003)

Area matching Drosophila EST AA438500 (inverted).

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: Pak3 CG14895

(Burbelo, 2001.7.27)

Source for merge of: Pak3 anon-WO0118547.285

(FlyBase, 1996-)

Additional comments

Source for merge of Pak3 anon-WO0118547.285 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG14895

(Hosono et al., 2015, Llamusi et al., 2013, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Molnar et al., 2006, Laviolette et al., 2005, Dan et al., 2001)

D-Pak3

(Mentzel et al., 2003)

DPAK3

(Chen et al., 2004)

DPak3

(Lee and Chen, 2019, Hofmann et al., 2004)

DmPAK3

(Mentzel et al., 2009, Mentzel and Raabe, 2005)

Dpak3

(Duan et al., 2012)

PAK-3

(Zheng et al., 2015)

PAK3

(Li et al., 2024, Zhou et al., 2022, Noyes et al., 2021, Morrison et al., 2000)

PaK3

(Bjorklund et al., 2006)

Pak

(Rout et al., 2022)

anon-WO0118547.285

dPAK3

(Burbelo, 2001.7.27, Pirone et al., 2001)

dpak3

(Duan et al., 2018, Duan et al., 2012)

pak3

(Ozdowski et al., 2011, Bahri et al., 2010, Davidson et al., 2009, Orian et al., 2003)

Name Synonyms

Pak3

p21-activated kinase 3

(Ozdowski et al., 2011)

Secondary FlyBase IDs

FBgn0038430
FBgn0062119

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 75 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41995

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4KGS4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4KGS4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41995/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0044826

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0044826

FlyMine - An integrated database for Drosophila genomics

FBgn0044826

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG14895

MARRVEL_MODEL - MARRVEL (model organism gene)

41995

Linkouts

BioGRID - A database of protein and genetic interactions.

67040

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0044826

DroID - A comprehensive database of gene and protein interactions.

FBgn0044826

DRSC - Results frm RNAi screens

FBgn0044826

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pak3_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0044826

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0044826

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14895

Flygut - An atlas of the Drosophila adult midgut

FBgn0044826

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002075

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Hippo signaling pathway - multiple species

MIST (genetic) - An integrated Molecular Interaction Database

41995

MIST (protein-protein) - An integrated Molecular Interaction Database

41995

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8IGZ0

References (109)

The following external sites may use different assemblies or annotations than FlyBase.

-0.89

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