FlyBase Gene Report: Dmel\Lk6

General Information

Symbol

Dmel\Lk6

Species

D. melanogaster

Name

Lk6 kinase

Annotation Symbol

CG17342

Feature Type

protein_coding_gene

FlyBase ID

FBgn0017581

Gene Model Status

Current

Stock Availability

34 publicly available

Gene Summary

Lk6 kinase (Lk6) encodes the single ortholog of MAPK signal integrating kinases, which are phosphorylated and activated by ERK. Lk6 product phosphorylates the protein encoded by eIF4E1 to influence translation initiation via formation of the eIF4F complex. [Date last reviewed: 2018-09-20] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

86E17-86E18

Sequence location

3R:11,750,931..11,764,457 [-]

Recombination map

3-51

RefSeq locus

NT_033777 REGION:11750931..11764457

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables kinase activity

inferred from mutant phenotype

(Arquier et al., 2005)

enables mitogen-activated protein kinase binding

inferred from physical interaction with FLYBASE:rl; FB:FBgn0003256

(Parra-Palau et al., 2005)

enables protein kinase activity

inferred from direct assay

(Kidd and Raff, 1997)

enables protein serine/threonine kinase activity

inferred from direct assay

(Parra-Palau et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in microtubule-based process

inferred from mutant phenotype

(Kidd and Raff, 1997)

involved_in regulation of growth

inferred from mutant phenotype

(Arquier et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in centrosome

inferred from direct assay

(Kidd and Raff, 1997)

located_in cytoplasm

inferred from direct assay

(Parra-Palau et al., 2005, Kidd and Raff, 1997)

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in microtubule

inferred from direct assay

(Kidd and Raff, 1997)

located_in nuclear envelope

inferred from direct assay

(Kidd and Raff, 1997)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -: The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).

Summary (Interactive Fly)

Serine/Threonine protein kinase that exerts a tight control on eIF4E phosphorylation and is necessary for normal growth and development

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Lk6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VGI4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Lk6-RA

FBtr0082534

NM_143729

6281

1142

Lk6-RB

FBtr0082535

NM_169423

4379

1090

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Lk6-PA

FBpp0082008

128.5

1142

4.94

FBpp0082009

123.4

1090

4.89

Q9VGI5

NP_731624

AAF54695

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lk6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Lk6_1
Lk6_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.27

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in centrosome

inferred from direct assay

(Kidd and Raff, 1997)

located_in cytoplasm

inferred from direct assay

(Parra-Palau et al., 2005, Kidd and Raff, 1997)

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in microtubule

inferred from direct assay

(Kidd and Raff, 1997)

located_in nuclear envelope

inferred from direct assay

(Kidd and Raff, 1997)

Expression Deduced from Reporters

Reporter: P{GawB}Lk6^DJ634

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

(Seroude, 2002)

fat body

(Kapahi et al., 2004, Seroude et al., 2002)

adult fat body

(Park et al., 2014)

Reporter: P{ST.lexA::HG}EJK-1

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

larval ventral nerve cord