Pk61C, dPDK1, Dstpk61, PDK, Protein kinase 61C
a master kinase that is crucial for the activation of AKT (PKB) and many kinases including PKC and S6K - contains pleckstrin homology (PH) domains that interact with the PtdIns(3,4,5)P second messenger - regulates cell growth - controls synaptic bouton size, active zone number, and synaptic function - essential for neuronal differentiation
Gene model reviewed during 5.55
Gene model reviewed during 5.46
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.56
The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pdk1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pdk1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene used to treat S2 cells.
dsRNA made from templates generated with primers directed against this gene inhibits TORC1-dependent S6k phosphorylation but does not inhibit Akt1 phosphorylation in S2 cells. In fact, knockdown of Pk61C increases TORC2-dependent Akt1 phosphorylation.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells, a decrease in cell size, a decrease in mitotic index is seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila EST AI292767. This EST matches Drosophila serine/threonine protein kinase gene, Acc. No. Y07908.