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Citation
Rust, K., Byrnes, L.E., Yu, K.S., Park, J.S., Sneddon, J.B., Tward, A.D., Nystul, T.G. (2020). A single-cell atlas and lineage analysis of the adult Drosophila ovary.  Nat. Commun. 11(1): 5628.
FlyBase ID
FBrf0247148
Publication Type
Research paper
Abstract

The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.

PubMed ID
PubMed Central ID
PMC7648648 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Commun.
    Title
    Nature communications
    ISBN/ISSN
    2041-1723
    Data From Reference
    Alleles (47)
    Genes (169)
    Natural transposons (1)
    Insertions (17)
    Experimental Tools (2)
    Transgenic Constructs (30)