zfh-1, zinc finger homeodomain 1, Zinc-finger homeodomain protein 1, l(3)00865
transcription factor - zinc finger domain and homeodomain - mutation results in various degrees of local errors in mesodermal cell fate or positioning - a transcription factor network, comprised of , and , induces the expression of the Unc5 and Beaten-path guidance receptors and the Fasciclin 2 and Neuroglian adhesion molecules to guide individual dorsal motor neuron axons
Gene model reviewed during 5.55
Low-frequency RNA-Seq exon junction(s) not annotated.
Stop-codon suppression (UAG) postulated; FBrf0216884.
Gene model reviewed during 5.44
Gene model reviewed during 5.56
7.5 (northern blot)
None of the polypeptides share 100% sequence identity.
1060 (aa); 145 (kD observed); 117 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\zfh1 using the Feature Mapper tool.
Comment: reported as pericardial cell specific anlage
zfh1 is expressed in neuroblasts along the ventral midline at embryonic stage 11 and then symmetrically along the midline at stages 13 and 15. It is then expressed in CNS neurons at stage 15.
Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.
Comment: in 10-50% of examined hemisegments
Comment: in 10-50% of examined hemisegments
zfh1 is expressed in muscle precursor cells in the wing disc. In early stages, expression is uniform but in later stages levels are reduced in cells with high ct expression that will give rise to the direct flight muscles. The cells in which zfh1 expression remains high give rise to the indirect flight muscles. zfh1 expression is observed in sparse nuclei embedded in the lamina in adult IFM. These are persistant muscle progenitor cells that remain undifferentiated in the adult. zfh1 is also expressed in another cell population in muscle that were identified as plasmatocytes.
zfh1 is expressed in all peripheral glial cells at embryonic stages 14 and 16 and in ePG12 but is excluded from other neurons and accessory cells.
zfh1 protein levels are high in cyst progenitor cells and then drop in cyst cell daughters.
Markers that uniquely identify the cells of the NB3-7 lineage were used to examine the serotonin expressing cell lineages.
zfh1 protein is initially expressed in a subset of heart precursor cells. Later expression is observed in all cells that form the dorsal vessel, cardial cells as well as pericardial cells.
zfh1 protein is abundant in staged extracts from embryos between 3 and 18 hours. zfh1 protein is first detected in the presumptive procephalic mesoderm and then in the ventral mesoderm and pole cells. In late stage 8, strong staining is seen in a ~30 cell-wide band of ventral mesoderm. In stage 11, zfh1 protein is detected mainly in ectodermally-derived CNS structures and is thought to be present in most identifiable motor neurons including the aCC, RP, VUM, ventral intersegmental, and lateral ipsisegmental neurons. In stage 13, zfh1 protein is abundant in the CNS as well as in some mesodermally derived structures, including the dorsal vessel, gonadal support cells, and laterally located adult muscle precursor cells of the thoracic and abdominal segments. At stage 15, two rows of dorsal vessel cardioblasts are strongly stained as dorsal closure is completed. The CNS has condensed and shows staining of segmentally repeated subsets of neurons. Weak staining persists in the somatic and visceral musculature.
GBrowse - Visual display of RNA-Seq signalsView Dmel\zfh1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: zfh1 CG1322
Expression is enriched in embryonic gonads.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.
zfh1 is specifically required for the formation of the eve-expressing subset of pericardial cells (EPCs), without affecting the formation of their siblings, the founders of a dorsal body wall muscle (DA1). zfh1 specifies EPC development independently of numb.
tin is not required for early zfh1 expression throughout the mesoderm or for the refinement of this expression to lateral mesodermal clusters during stage 10. tin activity is required for aspects of zfh1 expression beginning at stage 11.
Loss of zfh1 activity disrupts the development of two distinct mesodermal populations: the caudal visceral mesoderm and the gonadal mesoderm. Ectopic expression of zfh1 is sufficient to induce additional gonadal mesodermal cells and to alter the temporal course of gene expression within these cells.
zfh1 function is required for the transition of germ cells from the endoderm to the mesoderm, suggesting zfh1 is required for interactions between germ cells and somatic gonadal precursors (SGPs). Maternal zfh1 is not required for proper germ cell migration. zfh1 function is also required for aspects of caudal visceral mesoderm-specific gene expression and for the migration of these cells. tin and zfh1 cooperate in the specification of two tissues derived from the lateral mesoderm: the gonadal mesoderm and the fat body. zfh1 is a primary regulator of gonadal mesoderm, as it is both able to promote ectopic gonadal mesoderm formation and to alter the temporal course of gene expression in these cells.
Mutants are isolated in an EMS mutagenesis screen to identify zygotic mutations affecting germ cell migration at discrete points during embryogenesis: mutants exhibit germ cell migration defects.
zfh1 is required for the development of both fat body and gonadal mesoderm in the embryo.
Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.
One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus Engrailed binding site.
Phenotypic analysis reveals that the gene is not required for the initial segregation of the mesoderm or differentiation of mesodermally derived tissues, but loss of zfh1 function results in varying degrees of local errors in cell fate or positioning.
The product of zfh1 possesses one homeodomain and nine C2H2 zinc fingers. The novel arrangement of interspersed homeodomain and zinc-finger motifs in the primary sequences of the zfh1 and zfh2 gene products may signify an unusual mechanism of transcriptional regulation by these products.