FlyBase Gene Report: Dmel\jub

General Information

Symbol

Dmel\jub

Species

D. melanogaster

Name

Ajuba LIM protein

Annotation Symbol

CG11063

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030530

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

Ajuba LIM protein (jub) encodes a LIM domain-containing protein that binds to and inhibits activation of the Hippo pathway kinase encoded by wts. It localizes to adherens junctions in a tension-dependent manner. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
UniProtKB
Interactive Fly

Also Known As

Ajuba, djub

Key Links

Genomic Location

Cytogenetic map

12C6-12C6

Sequence location

X:13,826,041..13,830,317 [-]

Recombination map

1-46

RefSeq locus

NC_004354 REGION:13826041..13830317

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VA38

(Das Thakur et al., 2010)

inferred from physical interaction with UniProtKB:Q9VCR6

(Das Thakur et al., 2010)

enables protein sequestering activity

inferred from physical interaction with FLYBASE:wts; FB:FBgn0011739

(Rauskolb et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables transcription corepressor activity

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Jia et al., 2015)

involved_in mitotic cell cycle

inferred from mutant phenotype

(Sabino et al., 2011)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Huang et al., 2016, Fletcher et al., 2015, Sun and Irvine, 2013, Rauskolb et al., 2011, Das Thakur et al., 2010)

inferred from genetic interaction with FLYBASE:sqh; FB:FBgn0003514,FLYBASE:Rok; FB:FBgn0026181

(Rauskolb et al., 2014)

involved_in positive regulation of imaginal disc growth

inferred from mutant phenotype

(Rauskolb et al., 2011)

involved_in protein localization to centrosome

inferred from mutant phenotype

(Sabino et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

involved_in negative regulation of hippo signaling

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

involved_in regulation of DNA-templated transcription

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

involved_in response to hypoxia

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Rauskolb et al., 2014)

located_in adherens junction

inferred from direct assay

(Das Thakur et al., 2010)

located_in apical cortex

inferred from direct assay

(Sabino et al., 2011)

colocalizes_with apical part of cell

inferred from direct assay

(Rauskolb et al., 2014)

colocalizes_with pericentriolar material

inferred from direct assay

(Sabino et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in adherens junction

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

is_active_in P-body

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

part_of transcription regulator complex

inferred from biological aspect of ancestor with PANTHER:PTN001198456

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the zyxin/ajuba family. (Q9VY77)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Protein Function (UniProtKB)

Regulates organ size by inhibiting apoptosis and promoting cell proliferation by influencing the expression of G1/S-specific cyclin-E (CycE) and apoptosis 1 inhibitor (th). Negatively regulates the Hippo signaling pathway.

(UniProt, Q9VY77)

Summary (Interactive Fly)

Adherens junction protein - an upstream regulator of Hippo signaling that functions as a sensor of epithelial integrity - a negative regulator of Warts within the Hippo pathway - Jub localization to adherens junctions and its association with alpha-catenin are promoted by cytoskeletal tension - recruits Warts to junctions in a tension-dependent manner

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\jub for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VY77)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Apparent introns within coding exon in cDNAs GB:BT014658 (LD32332) and GB:AY070556 (LD3167) are likely to be artifacts; possibly caused by reverse transcriptase template-switching in regions of CAG repeats.

Gene model reviewed during 5.40

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

jub-RB

FBtr0073834

NM_132702

2634

728

jub-RC

FBtr0303418

NM_001201657

2604

718

jub-RD

FBtr0303419

NM_001201658

2619

723

jub-RE

FBtr0340292

NM_001298303

2893

718

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

jub-PB

FBpp0073665

78.0

728

7.50

FBpp0292479

76.9

718

7.22

FBpp0292480

77.4

723

7.22

FBpp0309253

76.9

718

7.22

M9MSC9

NP_001285232

AHN59702

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

718 aa isoforms: jub-PC, jub-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with sav and wts.

(UniProt, Q9VY77)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\jub using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

jub_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.57

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

interommatidial cell | punctate

• adherens junction

(Das Thakur et al., 2010)

Additional Descriptive Data

jub colocalizes with shg in a punctate pattern at adherens junctions, predominantly in interommadtidial cells.

(Das Thakur et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Rauskolb et al., 2014)

located_in adherens junction

inferred from direct assay

(Das Thakur et al., 2010)

located_in apical cortex

inferred from direct assay

(Sabino et al., 2011)

colocalizes_with apical part of cell

inferred from direct assay

(Rauskolb et al., 2014)

colocalizes_with pericentriolar material

inferred from direct assay

(Sabino et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\jub in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\jub

View exonic expression by tissue for Dmel\jub

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32351/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030530

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030530

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030530

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11063

Flygut - An atlas of the Drosophila adult midgut

FBgn0030530

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 23 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of jub

Transgenic constructs containing regulatory region of jub

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(1)jub^I

(Das Thakur et al., 2010)

Df(1)jub^II

(Das Thakur et al., 2010)

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

jub^EPG845

lethal - all die before end of prepupal stage

jub^E1

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PI

lethal - all die before end of pupal stage

jub⁵⁴

viable

jub^e03614

viable, with Scer\GAL4^69B

jub^EPG845

viable, with Scer\GAL4^Mef2.PR

jub^GD7185

viable, with Scer\GAL4^pnr-MD237

jub^GD7185

viable, with Scer\GAL4^tin.CΔ4

jub^GD7185

viable, with Scer\GAL4^αTub84B.PL

jub^EPG845

Sterility

Allele

fertile

jub^e03614

Other Phenotypes

Allele

abnormal cell adhesion | embryonic stage

jub⁵⁴

abnormal cell shape | larval stage, with Scer\GAL4^hh.PU

jub^HMS02335

abnormal mitotic cell cycle | somatic clone

jub^E1

abnormal size, with Scer\GAL4^nub-AC-62

decreased cell growth rate | somatic clone

jub^E1

decreased cell number, with Scer\GAL4^Bx-MS1096

jub^{RNAi.C.UAS.22.5}

decreased cell number, with Scer\GAL4^GMR.PF

jub^{RNAi.C.UAS.22.5}

increased cell death | third instar larval stage, with Scer\GAL4^en-e16E

jub^HMS02335

increased cell number, with Scer\GAL4^Bx-MS1096

jub^UAS.mCherry

increased cell number, with Scer\GAL4^GMR.PF

jub^UAS.mCherry

increased cell size | larval stage, with Scer\GAL4^hth-GETDB

jub^GD7185

some die during pharate adult stage, with Scer\GAL4^Act5C.PI

some die during prepupal stage | recessive

jub^E1

visible, with Scer\GAL4^nub-AC-62

jub^RNAi.UAS.cUa

visible | adult stage, with Scer\GAL4^nub.PU

jub^HMS02335

Phenotype manifest in

Allele

amnioserosa | dorsal closure stage

jub⁵⁴

antennal disc | larval stage, with Scer\GAL4^hth-GETDB

jub^GD7185

centrosome | somatic clone

jub^E1

embryonic epidermis

jub⁵⁴

embryonic epidermis | dorsal closure stage

jub⁵⁴

embryonic leading edge cell | dorsal closure stage

jub⁵⁴

epidermal cell | embryonic stage

jub⁵⁴

epithelial cell of wing disc | larval stage, with Scer\GAL4^hh.PU

jub^HMS02335

eye, with Scer\GAL4^GMR.PF

jub^UAS.mCherry

eye | pupal stage, with Scer\GAL4^GMR.PF

jub^{RNAi.C.UAS.22.5}

neuroblast | somatic clone

jub^E1

spindle | somatic clone

jub^E1

wing, with Scer\GAL4^nub.PU

jub^HMS02335

wing, with Scer\GAL4^nub-AC-62

wing blade, with Scer\GAL4^Bx-MS1096

wing disc | larval stage, with Scer\GAL4^hh.PU

jub^HMS02335

wing disc | third instar larval stage, with Scer\GAL4^en-e16E

jub^HMS02335

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (76)

Hsap\LIMD1

9 of 14

Yes

Hsap\WTIP

9 of 14

Yes

Hsap\AJUBA

4 of 14

Yes

Hsap\ZYX

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (30)

Rnor\Limd1

9 of 14

Yes

Rnor\Wtip

8 of 14

Yes

Rnor\Ajuba

4 of 14

Yes

Rnor\Lpp

3 of 14

Rnor\Zyx

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tesl

1 of 14

Rnor\Tgfb1i1

1 of 14

Mus musculus (laboratory mouse) (34)

Mmus\Wtip

9 of 14

Yes

Mmus\Limd1

8 of 14

Yes

Mmus\Ajuba

5 of 14

Yes

Mmus\Lpp

3 of 14

Mmus\Zyx

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tesl1

1 of 14

Mmus\Tgfb1i1

1 of 14

Mmus\Zfp185

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (40)

Xtro\limd1

6 of 13

Yes

Xtro\wtip

4 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (38)

Drer\wtip

9 of 14

Yes

Drer\ajuba

4 of 14

Yes

Drer\limd1a

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\lmo7b

1 of 14

Yes

Drer\LOC110439375

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\tes

1 of 14

Drer\trip6

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (25)

Cele\zyx-1

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (32)

Agam\AgaP_AGAP004548

9 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\PLIM2a

1 of 13

Yes

Atha\PLIM2b

1 of 13

Yes

Atha\PLIM2c

1 of 13

Yes

Atha\WLIM1

1 of 13

Yes

Atha\WLIM2a

1 of 13

Yes

Atha\WLIM2b

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\RGA1

1 of 13

Yes

Scer\RGA2

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\pxl1

1 of 12

Yes

Spom\rga3

1 of 12

Yes

Spom\rga4

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:jub. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (36)

Zyx

4 of 13

esn

2 of 13

stck

2 of 13

Tes

2 of 13

1 of 13

Awh

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Dll

1 of 13

ems

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Pax

1 of 13

Pph13

1 of 13

Rassf

1 of 13

smash

1 of 13

tup

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

jub^HMS02335

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LIMD1; LIM domain containing 1

9 of 14

604543

WTIP; WT1 interacting protein

9 of 14

614790

AJUBA; ajuba LIM protein

4 of 14

609066

ZYX; zyxin

3 of 14

602002

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

jub

Hsap\LIMD1

(Das Thakur et al., 2010)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

jub - Hecw

pull down, Identification by mass spectrometry

(Fajner et al., 2021)

jub - Pi3K21B

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

jub - dachs

anti tag coimmunoprecipitation, anti tag western blot

(Rauskolb et al., 2011)

jub - drk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

jub - sav

anti tag coimmunoprecipitation, anti tag western blot

(Reddy and Irvine, 2013)

jub - step

anti tag coimmunoprecipitation, anti tag western blot

(Rauskolb et al., 2022)

jub - wts

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Rauskolb et al., 2022, Alégot et al., 2019, Rauskolb et al., 2014, Reddy and Irvine, 2013)

jub - α-Cat

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Rauskolb et al., 2022, Alégot et al., 2019, Rauskolb et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

jub

enhanceable

aurA

(Sabino et al., 2011)

jub

suppressible

aurA

(Sabino et al., 2011)

jub

enhanceable

shg

(Razzell et al., 2018)

jub

enhanceable

Zyx

(Rauskolb et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cno

enhanceable

jub

(Sheppard et al., 2023)

suppressible

jub

(Rauskolb et al., 2011)

suppressible

jub

(Rauskolb et al., 2011)

Rok

suppressible

jub

(Rauskolb et al., 2014)

Rok

enhanceable

jub

(Rauskolb et al., 2014)

Zyx

enhanceable

jub

(Rauskolb et al., 2011)

α-Cat

enhanceable

jub

(Sheppard et al., 2023)

External Data

Subunit Structure (UniProtKB)

Interacts with sav and wts.

(UniProt, Q9VY77 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58722

DroID - A comprehensive database of gene and protein interactions.

FBgn0030530

MIST (genetic) - An integrated Molecular Interaction Database

32351

MIST (protein-protein) - An integrated Molecular Interaction Database

32351

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-46

Cytogenetic map

12C6-12C6

Sequence location

X:13,826,041..13,830,317 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

12C6-12C6

Limits computationally determined from genome sequence between P{EP}CG33174^EP1101 and P{EP}EP1595^EP1595

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-46

(FlyBase, 2016)

1-44.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

600184

w^*; P{jub-GFP}1

56806

w^*; P{jub-GFP}18A/TM2

30806

P{GawB}Bx^MS1096; P{UAS-jub.RNAi.22.5}3

41938

y¹ v¹; P{TRiP.HMS02335}attP40

600160

w^* jub^E1 P{FRT(w^hs)}101/FM7a

83240

y¹ w^* TI{CRIMIC.TG4.1}jub^{CR00993-TG4.1}/FM7h

78677

y¹ sc^* v¹ sev²¹; P{TKO.GS01952}attP40

78750

y¹ sc^* v¹ sev²¹; P{TOE.GS02289}attP40

NIG-Fly

HMS02335

y¹ v¹; P{TRiP.HMS02335}attP40/CyO

HMJ22341

y¹ v¹; P{TRiP.HMJ22341}attP40/CyO

VDRC

v101993

P{KK108565}VIE-260B

v38442

w¹¹¹⁸; P{GD7185}v38442

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (25)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE30166

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030530

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030530

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

jub is required to maintain aur at the centrosome.

(Sabino et al., 2011)

jub is associated with the pericentriolar material, but only in some tissues, notably the mitotic cells within the larval brain.

(Sabino et al., 2011)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: jub CG11063

(Das Thakur et al., 2010)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

Ajuba

(Perez-Vale and Peifer, 2020, van Soldt and Cardoso, 2020, Razzell et al., 2018, Meister, 2013)

CG11063

(National Institute of Genetics Fly Stocks, 2023-, Meadows, 2014.12.12, Glatter et al., 2011, Sabino et al., 2011, Das Thakur et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Bakal et al., 2007)

Djub

(Gilhaus et al., 2023)

JUB

(Harvey and Hariharan, 2012)

dJub

(Gogia et al., 2021, Schimizzi and Longmore, 2015, Liu et al., 2012, Zhao et al., 2011)

djub

(Jagannathan et al., 2016, Enomoto et al., 2015, Das Thakur et al., 2010)

Name Synonyms

Ajuba

(Mohammadi et al., 2020, Ahmed-de-Prado and Baonza, 2018, Fletcher et al., 2015, Sabino et al., 2011)

Ajuba LIM

(van Soldt and Cardoso, 2020)

Ajuba LIM protein

(Transgenic RNAi Project members, 2017-)

Drosophila Ajuba LIM protein

(Das Thakur et al., 2010)

ajuba

(Bulgakova et al., 2012)

djub

(Bao et al., 2011)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32351

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VY77

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VY77

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VY77

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32351/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030530

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030530

FlyMine - An integrated database for Drosophila genomics

FBgn0030530

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11063

MARRVEL_MODEL - MARRVEL (model organism gene)

32351

Linkouts

BioGRID - A database of protein and genetic interactions.

58722

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030530

DroID - A comprehensive database of gene and protein interactions.

FBgn0030530

DRSC - Results frm RNAi screens

FBgn0030530

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

jub_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030530

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030530

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11063

Flygut - An atlas of the Drosophila adult midgut

FBgn0030530

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0030530

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013882

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

32351

MIST (protein-protein) - An integrated Molecular Interaction Database

32351

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha

References (109)

The following external sites may use different assemblies or annotations than FlyBase.

-0.57

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