FlyBase Gene Report: Dmel\Mlp84B

General Information

Symbol

Dmel\Mlp84B

Species

D. melanogaster

Name

Muscle LIM protein at 84B

Annotation Symbol

CG1019

Feature Type

protein_coding_gene

FlyBase ID

FBgn0014863

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Muscle LIM protein at 84B (Mlp84B) encodes a muscle cytoskeletal protein found primarily at the Z-line boundary. It maintains muscle structural integrity and regulates actin bundling. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

DmLIM-3

Key Links

Genomic Location

Cytogenetic map

84C1-84C1

Sequence location

3R:7,112,850..7,117,732 [-]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:7112850..7117732

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of muscle

inferred from mutant phenotype

(Clark et al., 2007)

inferred from genetic interaction with FLYBASE:sls; FB:FBgn0086906

(Clark et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables actinin binding

inferred from biological aspect of ancestor with PANTHER:PTN001198226

(GO Reference Genome Project, 2011-)

enables structural constituent of muscle

inferred from biological aspect of ancestor with PANTHER:PTN001198226

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in muscle tissue development

inferred from expression pattern

(Stronach et al., 1996)

involved_in sarcomere organization

inferred from genetic interaction with FLYBASE:sls; FB:FBgn0086906

(Clark et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in muscle tissue development

inferred from biological aspect of ancestor with PANTHER:PTN001198226

(GO Reference Genome Project, 2011-)

involved_in sarcomere organization

inferred from biological aspect of ancestor with PANTHER:PTN001198226

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

colocalizes_with actin cytoskeleton

inferred from direct assay

(Stronach et al., 1996)

located_in nucleus

inferred from direct assay

(Clark et al., 2007)

located_in Z disc

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(Clark et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001198224

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001198226

(GO Reference Genome Project, 2011-)

is_active_in Z disc

inferred from biological aspect of ancestor with PANTHER:PTN001198226

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Zinc finger, LIM-type

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Plays a role in cell differentiation late in myogenesis. Transcription factor Mef2 is essential for expression.

(UniProt, Q24400)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mlp84B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24400)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.47

Gene model reviewed during 6.02

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mlp84B-RA

FBtr0081616

NM_057774

2399

495

Mlp84B-RB

FBtr0081617

NM_169173

2382

495

Mlp84B-RC

FBtr0346967

NM_001316489

3071

495

Additional Transcript Data and Comments

Reported size (kB)

2.3 (northern blot)

(Stronach et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mlp84B-PA

FBpp0081133

53.5

495

8.32

FBpp0081134

53.5

495

8.32

FBpp0312428

53.5

495

8.32

Q24400

NP_001303418

ALI30537

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

495 aa isoforms: Mlp84B-PA, Mlp84B-PB, Mlp84B-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

495 (aa); 53 (kD)

(Stronach et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Zinc finger, LIM-type

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q24400

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mlp84B using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mlp84B_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.59

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

larval muscle system

(Fisher et al., 2012)

embryonic stage 16

larval somatic muscle cell

(Stronach et al., 1996)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

pharyngeal muscle cell

(Stronach et al., 1996)

embryonic stage -- adult stage

(Stronach et al., 1996)

embryonic stage 14 -- 17

visceral trunk mesoderm

(Stronach et al., 1996)

somatic trunk mesoderm

(Stronach et al., 1996)

Additional Descriptive Data

Mlp84B transcripts are first detected by northern blot analysis in 8-12hr embryos, peak in 16-24hr embryos, are present in low levels in larvae, show a second peak of expression at the larval to pupal transition, and persist in adults. They are detected by in situ hybridization starting in embryonic stage 14 in the growing syncytial myotubes. They are present in the completed pattern of somatic muscles in stage 16 embryos. Mlp84B transcripts are concentrated at the terminal portions of the myotubes near where they are making attachments to the epidermis. Transcripts are also expressed in the visceral mesoderm and the pharyngeal muscle. In contrast to the somatic muscle, the distribution of transcripts is not polarized in these locations.

(Stronach et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

muscle attachment site

(Stronach et al., 1996)

larval visceral muscle cell

(Stronach et al., 1996)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

larval somatic muscle cell

(Stronach et al., 1996)

pharyngeal muscle cell

(Stronach et al., 1996)

embryonic stage 16

larval visceral muscle cell

(Stronach et al., 1999)

larval somatic muscle cell

(Stronach et al., 1999)

pharyngeal muscle cell

(Stronach et al., 1999)

muscle attachment site

(Stronach et al., 1999)

larval stage

larval visceral muscle cell

• Z disc

(Stronach et al., 1999)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Mlp84B protein is detected beginning in stage 14 embryos in somatic, visceral and pharyngeal muscles. Protein is found throughout the myotube but is more concentrated at the ends of muscle fibers at the point of attachment to the epidermis. Mlp84B protein appears to associate with linear cytoplasmic elements in the muscle cell syncytium that are thought to be the sarcomeric actin filament network.

(Stronach et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with actin cytoskeleton

inferred from direct assay

(Stronach et al., 1996)

located_in nucleus

inferred from direct assay

(Clark et al., 2007)

located_in Z disc

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(Clark et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mlp84B in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mlp84B

View exonic expression by tissue for Dmel\Mlp84B

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014863

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40849/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014863

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014863

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014863

Flygut - An atlas of the Drosophila adult midgut

FBgn0014863

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mlp84B

Transgenic constructs containing regulatory region of Mlp84B

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(3R)Antp-X1

(Clark et al., 2007)

Df(3R)dsx2M

(Clark et al., 2007, Perrin and Dura, 2004)

Df(3R)Mlp84B^P8

(Clark et al., 2007)

Df(3R)Scx2

(Clark et al., 2007, Perrin and Dura, 2004)

Df(3R)Scx4

(Clark et al., 2007)

Gene partially disrupted in

Df(3R)dsx2M

(Clark et al., 2007)

Gene not disrupted in

Df(3R)Antp2

(Clark et al., 2007)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(3R)dsx2M)

lethal (with Df(3R)Scx2)

lethal (with Mlp84B^y-30)

Mlp84B^y-37

lethal (with Mlp84B^y-37)

Mlp84B^y-30

lethal | adult stage (with Df(3R)dsx2M)

Mlp84B^P20

lethal | P-stage (with Df(3R)dsx2M)

Mlp84B^P20

lethal | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mlp84B^GD6893

viable, with Scer\GAL4^Mef2.PR

Mlp84B^GD6893

viable, with Scer\GAL4^pnr-MD237

Mlp84B^GD6893

viable, with Scer\GAL4^tin.CΔ4

Mlp84B^GD6893

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

Mlp84B^GD6893

Phenotype manifest in

Allele

embryonic/larval hypodermal muscle cell | larval stage

Mlp84B^{GFP,Tag:NLS(SV40-largeT)}

filamentous actin

Mlp84B^PΔ8-95

filamentous actin | larval stage

Mlp84B^{GFP,Tag:NLS(SV40-largeT)}

mesonotum, with Scer\GAL4^pnr-MD237

Mlp84B^GD6893

nucleus | larval stage

Mlp84B^{GFP,Tag:NLS(SV40-largeT)}

pupal cuticle (with Df(3R)dsx2M)

Mlp84B^P20

trichogen cell, with Scer\GAL4^pnr-MD237

Mlp84B^GD6893

visceral muscle fiber

Mlp84B^PΔ8-95

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (67)

Hsap\CSRP2

10 of 14

Yes

Hsap\CSRP3

9 of 14

Yes

Hsap\CSRP1

8 of 14

Yes

Hsap\CRIP2

5 of 14

Yes

Hsap\CRIP3

4 of 14

Yes

Hsap\CRIP1

3 of 14

Hsap\LIMA1

2 of 14

Yes

Hsap\LIMD2

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (34)

Rnor\Csrp2

10 of 14

Yes

Rnor\Csrp3

10 of 14

Yes

Rnor\Csrp1

9 of 14

Yes

Rnor\Crip2

5 of 14

Yes

Rnor\Crip1

4 of 14

Rnor\Crip3

4 of 14

Yes

Rnor\RGD1563606

3 of 14

Yes

Rnor\Limd2

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (35)

Mmus\Csrp2

10 of 14

Yes

Mmus\Csrp3

10 of 14

Yes

Mmus\Csrp1

8 of 14

Yes

Mmus\Crip2

5 of 14

Yes

Mmus\Crip1

4 of 14

Mmus\Crip3

4 of 14

Yes

Mmus\Lima1

2 of 14

Yes

Mmus\Limd2

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (39)

Xtro\csrp3

7 of 13

Yes

Xtro\csrp1

6 of 13

Yes

Xtro\csrp2

6 of 13

Yes

Xtro\crip2

3 of 13

Yes

Xtro\crip1

2 of 13

Yes

Xtro\crip3

2 of 13

Yes

Xtro\foxe3

2 of 13

Xtro\LOC101734738

2 of 13

Yes

Xtro\c4h3orf84

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\snx33

1 of 13

Xtro\tes

1 of 13

Danio rerio (Zebrafish) (42)

Drer\csrp1a

10 of 14

Yes

Drer\csrp1b

9 of 14

Yes

Drer\csrp2

9 of 14

Yes

Drer\csrp3

9 of 14

Yes

Drer\crip2

5 of 14

Yes

Drer\crip2l

5 of 14

Yes

Drer\crip1

4 of 14

Drer\crip3

4 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\limd2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (25)

Cele\mlp-1

8 of 14

Yes

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\zyx-1

1 of 14

Anopheles gambiae (African malaria mosquito) (26)

Agam\AgaP_AGAP012305

12 of 12

Yes

3 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\PLIM2a

5 of 13

Yes

Atha\PLIM2b

5 of 13

Yes

Atha\PLIM2c

5 of 13

Yes

Atha\WLIM1

5 of 13

Yes

Atha\WLIM2a

5 of 13

Yes

Atha\WLIM2b

4 of 13

Yes

Atha\HDG1

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\LRG1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rga1

1 of 12

Yes

Spom\rga4

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mlp84B. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

8 of 13

3 of 13

3 of 13

2 of 13

1 of 13

Awh

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

esn

1 of 13

Hil

1 of 13

jub

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Pax

1 of 13

stck

1 of 13

Tes

1 of 13

Unc-115a

1 of 13

Unc-115b

1 of 13

Zasp52

1 of 13

zen

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

CSRP3; cysteine and glycine rich protein 3

model of dilated cardiomyopathy 1M

IEA

(FlyBase, 2019-)

model of hypertrophic cardiomyopathy 12

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CSRP2; cysteine and glycine rich protein 2

10 of 14

601871

CSRP3; cysteine and glycine rich protein 3

9 of 14

600824

CSRP1; cysteine and glycine rich protein 1

8 of 14

123876

CRIP2; cysteine rich protein 2

5 of 14

601183

CRIP3; cysteine rich protein 3

4 of 14

CRIP1; cysteine rich protein 1

3 of 14

123875

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mlp84B - Actn

anti tag coimmunoprecipitation, western blot, two hybrid

(Clark and Kadrmas, 2013)

Mlp84B - Lmpt

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot

(She et al., 2022)

Mlp84B - Rab10

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Mlp84B

enhanceable

sls

(Clark et al., 2007)

Mlp84B

suppressible

(Domsch et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Actn

enhanceable

Mlp84B

(Clark and Kadrmas, 2013)

dgo

enhanceable

Mlp84B

(Weber et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66046

DroID - A comprehensive database of gene and protein interactions.

FBgn0014863

MIST (protein-protein) - An integrated Molecular Interaction Database

40849

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84C1-84C1

Sequence location

3R:7,112,850..7,117,732 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84C1-84C1

Limits computationally determined from genome sequence between P{lacW}l(3)L2100^L2100 and P{EP}EP3060^EP3060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84B-84C

(Stronach, 1998.9.9)

84B-84B

(Beckerle, 1995.9.9)

84B3-84C6

(determined by in situ hybridisation)

(Stronach et al., 1996)

84B-84C

(determined by in situ hybridisation)

(Stronach, 1996.2.12)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

(Beckerle, 1995.9.9)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

66563

w¹¹¹⁸ P{Mlp84B-GFP}5A

66565

w¹¹¹⁸; P{Mlp84B-GFP.NLS}12-1

66564

w¹¹¹⁸; P{Mlp84B-GFP.NES}33A/TM3, Sb¹

8632

w¹¹¹⁸; wg^Sp-1/CyO; P{Mlp84B^+t10}3

26397

w¹¹¹⁸; Mi{ET1}Alh^MB08677 Mlp84B^MB08677

63332

w¹¹¹⁸; PBac{IT.GAL4}Alh^0457-G4 Mlp84B^0457-G4

60936

y¹ w^*; Mi{MIC}Alh^MI14261 Mlp84B^MI14261

8643

y¹ w^67c23; Df(3R)Mlp84B^y-30, Alh^y-30, Mlp84B^y-30/TM6B, Tb⁺

1019R-3

v108344

v18593

w¹¹¹⁸; P{GD6893}v18593

v318177

PBac{fTRG00678.sfGFP-TVPTBF}VK00033

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (86)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE40142

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0014863

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0014863

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Clark et al., 2007, Stronach et al., 1996)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

tn, Actn, sls and Mlp84B act in concert to maintain the integrity and stability of Z-discs, thereby ensuring myofibril stability and optimal muscle function.

(Domsch et al., 2013)

Mlp84B protein is identified and developmental expression pattern determined. Results reveal a striking conservation of sequence, timing of gene expression, tissue distribution of gene products and subcellular localisation among LIM proteins of vertebrates and invertebrates.

(Stronach et al., 1996)

Protein has five LIM domains and is expressed in the muscle.

(Stronach, 1996.2.12)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Mlp84B CG1019

(Zhao, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CG1019

(Rosa-Ferreira et al., 2015, Weber et al., 2012, Cammarato et al., 2011, Zhang et al., 2010, Keleman et al., 2009.8.5, Ni et al., 2008)

DLim-3

DmLIM-3

(Stronach et al., 1996, Stronach et al., 1995, Stronach et al., 1994)

MLP

(Labeau-Dimenna et al., 2012)

MLP84B

(Nir et al., 2012, Ibrahim et al., 2011)

Mlp

(Weber et al., 2012)

Mlp84b

(Dialynas et al., 2010)

Mlμ84B

(Bahri et al., 2001)

dMlp84B

(Weiskirchen and Gunther, 2003)

mlp

(Clark et al., 2007)

mlp84B

(Domsch et al., 2013, Clark and Beckerle, 2008, Clark et al., 2007, Clark et al., 2007, Clark et al., 2005, Clark et al., 2001)

mlp84b

(Marcogliese et al., 2017)

Name Synonyms

LIM protein 84B

(Labeau-Dimenna et al., 2012)

Muscle LIM protein

(Weber et al., 2012)

Muscle LIM protein at 84B

(Cammarato et al., 2011, Kapelnikov et al., 2008, Sandmann et al., 2006)

Muscle-specific LIM protein at 84B

(Stronach et al., 1996)

Secondary FlyBase IDs

FBgn0011644

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40849

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24400

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24400

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24400

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014863

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40849/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014863

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014863

FlyMine - An integrated database for Drosophila genomics

FBgn0014863

InterPro - A database of protein families, domains and functional sites

Zinc finger, LIM-type

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1019

MARRVEL_MODEL - MARRVEL (model organism gene)

40849

Linkouts

BioGRID - A database of protein and genetic interactions.

66046

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0014863

DroID - A comprehensive database of gene and protein interactions.

FBgn0014863

DRSC - Results frm RNAi screens

FBgn0014863

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mlp84B_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014863

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0014863

Flygut - An atlas of the Drosophila adult midgut

FBgn0014863

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007136

MIST (protein-protein) - An integrated Molecular Interaction Database

40849

References (95)

The following external sites may use different assemblies or annotations than FlyBase.

-0.59

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