FlyBase Gene Report: Dmel\CG33521

General Information

Symbol

Dmel\CG33521

Species

D. melanogaster

Name

Annotation Symbol

CG33521

Feature Type

protein_coding_gene

FlyBase ID

FBgn0250819

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

BcDNA:RE73965

Key Links

Genomic Location

Cytogenetic map

102F8-102F8

Sequence location

4:1,185,768..1,192,985 [+]

Recombination map

4-0

RefSeq locus

NC_004353 REGION:1185768..1192985

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in actin cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN001198224

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001198224

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Zinc finger, LIM-type

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG33521 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q59DP4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.39

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG33521-RA

FBtr0091472

NM_001014703

2414

663

CG33521-RC

FBtr0091470

NM_001014705

2529

649

CG33521-RD

FBtr0091473

NM_001014702

2870

649

CG33521-RG

FBtr0334661

NM_001258506

2051

586

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG33521-PA

FBpp0090952

76.4

663

8.61

FBpp0090950

74.8

649

8.09

FBpp0090953

74.8

649

8.09

FBpp0306723

67.6

586

8.84

H9XVQ0

NP_001245435

AFH06795

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

649 aa isoforms: CG33521-PC, CG33521-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Zinc finger, LIM-type

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG33521 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG33521 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG33521

View exonic expression by tissue for Dmel\CG33521

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250819

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3346141/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250819

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250819

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33521

Flygut - An atlas of the Drosophila adult midgut

FBgn0250819

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG33521

Transgenic constructs containing regulatory region of CG33521

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(4)G

(Podemski et al., 2004)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

CG33521^GD13566

viable, with Scer\GAL4^pnr-MD237

CG33521^GD13566

Sterility

Allele

female fertile

CG33521^C

female fertile (with Df(4)C3)

CG33521^C

female fertile (with Df(4)sc^H)

CG33521^C

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (34)

Hsap\LIMA1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Crip1

1 of 14

Yes

Rnor\Crip2

1 of 14

Yes

Rnor\Crip3

1 of 14

Yes

Rnor\Csrp1

1 of 14

Yes

Rnor\Csrp2

1 of 14

Yes

Rnor\Csrp3

1 of 14

Yes

Rnor\Lasp1

1 of 14

Yes

Rnor\Lima1

1 of 14

Yes

Rnor\Limd2

1 of 14

Yes

Rnor\Mical1

1 of 14

Yes

Rnor\Mical2

1 of 14

Yes

Rnor\Mical3

1 of 14

Yes

Rnor\Micall2

1 of 14

Yes

Rnor\RGD1563606

1 of 14

Yes

Mus musculus (laboratory mouse) (14)

Mmus\Crip1

1 of 14

Yes

Mmus\Crip2

1 of 14

Yes

Mmus\Crip3

1 of 14

Yes

Mmus\Csrp1

1 of 14

Yes

Mmus\Csrp2

1 of 14

Yes

Mmus\Csrp3

1 of 14

Yes

Mmus\Lasp1

1 of 14

Yes

Mmus\Lima1

1 of 14

Yes

Mmus\Limd2

1 of 14

Yes

Mmus\Mical1

1 of 14

Yes

Mmus\Mical2

1 of 14

Yes

Mmus\Mical3

1 of 14

Yes

Mmus\Micall2

1 of 14

Yes

Mmus\Xirp2

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (18)

Xtro\crybb2

1 of 13

Yes

Xtro\csrp3

1 of 13

Yes

Xtro\fhl2

1 of 13

Yes

Xtro\fhl3

1 of 13

Yes

Xtro\lasp1

1 of 13

Yes

Xtro\ldb3

1 of 13

Yes

Xtro\lhx1

1 of 13

Yes

Xtro\lhx9

1 of 13

Yes

Xtro\lima1

1 of 13

Yes

Xtro\limd1

1 of 13

Yes

Xtro\lmx1a

1 of 13

Yes

Xtro\lmx1b.2

1 of 13

Yes

Xtro\mical3

1 of 13

Yes

Xtro\pdlim5

1 of 13

Yes

Xtro\prickle1

1 of 13

Yes

Xtro\prickle4

1 of 13

Yes

Xtro\prrx2

1 of 13

Yes

Xtro\pxn

1 of 13

Yes

Danio rerio (Zebrafish) (18)

Drer\crip1

1 of 14

Yes

Drer\crip2

1 of 14

Yes

Drer\crip2l

1 of 14

Yes

Drer\crip3

1 of 14

Yes

Drer\csrp1a

1 of 14

Yes

Drer\csrp1b

1 of 14

Yes

Drer\csrp2

1 of 14

Yes

Drer\csrp3

1 of 14

Yes

Drer\lasp1

1 of 14

Yes

Drer\lima1a

1 of 14

Yes

Drer\limd2

1 of 14

Yes

Drer\LOC556668

1 of 14

Yes

Drer\mical1

1 of 14

Yes

Drer\mical2b

1 of 14

Yes

Drer\mical3a

1 of 14

Yes

Drer\mical3b

1 of 14

Yes

Drer\micall1a

1 of 14

Yes

Drer\zgc:195282

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\tag-273

4 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP000149

1 of 12

Yes

Agam\AgaP_AGAP000222

1 of 12

Yes

Agam\AgaP_AGAP000731

1 of 12

Yes

Agam\AgaP_AGAP004256

1 of 12

Yes

Agam\AgaP_AGAP004548

1 of 12

Yes

Agam\AgaP_AGAP005020

1 of 12

Yes

Agam\AgaP_AGAP005400

1 of 12

Yes

Agam\AgaP_AGAP005425

1 of 12

Yes

Agam\AgaP_AGAP006526

1 of 12

Yes

Agam\AgaP_AGAP006537

1 of 12

Yes

Agam\AgaP_AGAP006540

1 of 12

Yes

Agam\AgaP_AGAP006901

1 of 12

Yes

Agam\AgaP_AGAP008532

1 of 12

Yes

Agam\AgaP_AGAP010391

1 of 12

Yes

Agam\AgaP_AGAP011136

1 of 12

Yes

Agam\AgaP_AGAP012305

1 of 12

Yes

Agam\PRIC1_ANOGA

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (6)

Atha\PLIM2a

2 of 13

Yes

Atha\PLIM2b

2 of 13

Yes

Atha\PLIM2c

2 of 13

Yes

Atha\WLIM1

2 of 13

Yes

Atha\WLIM2a

2 of 13

Yes

Atha\WLIM2b

2 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\LRG1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rga1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG33521. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (21)

Mlp84B

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

esn

1 of 13

Hil

1 of 13

jub

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Pax

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0250819

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

4-0

Cytogenetic map

102F8-102F8

Sequence location

4:1,185,768..1,192,985 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102F8-102F8

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

4-0

(FlyBase, 2016)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

60857

y¹; Mi{MIC}CG33521^MI13031

99877

y¹ w¹¹¹⁸; PBac{UAS-CG33521.HA}VK00037

99878

y¹ w¹¹¹⁸; PBac{UAS-CG33521.HA}VK00033

605317

y¹ w¹¹¹⁸; TI{TI}FRT101F CG33521^C

93691

y¹ w¹¹¹⁸; Mi{DH.0}CG33521^{MI13031-DH.GT-TG4.0}

Kyoto

119087

y¹ w¹¹¹⁸; PBac{UAS-CG33521.HA}VK00037

119088

y¹ w¹¹¹⁸; PBac{UAS-CG33521.HA}VK00033

119266

y¹ w¹¹¹⁸; TI{TI}FRT101F CG33521^C

118889

y¹ w¹¹¹⁸; Mi{DH.0}CG33521^{MI13031-DH.GT-TG4.0}

17467R-1

v104134

v48469

w¹¹¹⁸; P{GD16080}v48469

More stocks available...

Genomic Clones (7)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (81)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0250819

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RH47778

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0250819

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Annotation CG17467 was eliminated in release 3 of the genome annotation, October 2002. New annotation (CG32015) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Quantifying rates of protein sequence divergence within and between species reveals that the Drosophila genome harbors a substantial proportion of genes with a very high divergence rate.

(Schmid and Tautz, 1997)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG17467 anon- EST:fe2A9 anon- EST:fe2E7

(Bayraktaroglu, 2000.11.2)

Source for merge of: CG17467 CG32015

(FlyBase, 1996-)

Source for merge of: CG33521 BcDNA:RE73965

(FlyBase, 1996-)

Additional comments

Release 4.1 annotation CG33521 contains sequences corresponding to both release 3.2 annotation CG32015 and release 2 annotation CG17467.

(FlyBase Genome Annotators, 2005)

Source for merge of CG17467 CG32015 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: anon-fe2D9 CG17467

(Gong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

2D9

(Tautz and Schmid, 1998)

BcDNA:RE73965

CG17467

(Celniker et al., 2002)

CG32015

CG33521

(Fourth Chromosome Resource Project and Newfeld, 2022-, She et al., 2021, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5)

anon-EST:fe2A9

anon-EST:fe2D9

anon-EST:fe2E7

anon2D9

(Schmid, 1997.5.30)

Name Synonyms

BcDNA:RE73965

anon-fast-evolving-2A9

anon-fast-evolving-2D9

anon-fast-evolving-2E7

Secondary FlyBase IDs

FBgn0053521
FBgn0022341
FBgn0025215
FBgn0025232
FBgn0052015
FBtr0089197
FBpp0088261
FBtr0089198
FBpp0088262
FBtr0089199
FBpp0088263
FBtr0089200
FBpp0088264
FBgn0062975

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3346141

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q59DP4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q59DP4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250819

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3346141/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250819

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250819

InterPro - A database of protein families, domains and functional sites

Zinc finger, LIM-type

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33521

MARRVEL_MODEL - MARRVEL (model organism gene)

3346141

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0250819

DroID - A comprehensive database of gene and protein interactions.

FBgn0250819

DRSC - Results frm RNAi screens

FBgn0250819

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0250819

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33521

Flygut - An atlas of the Drosophila adult midgut

FBgn0250819

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006112

References (28)

The following external sites may use different assemblies or annotations than FlyBase.

-0.67

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