PDLP, Dpax, DPxn37
cytoskeletal adaptor that couples integrins to the actin cytoskeleton in focal adhesions - positively regulates Rac and negatively regulating Rho - regulates actin dynamics and cell adhesion during muscle fusion - targeted by JNK in the regulation of border-cell cluster integrity during oogenesis
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.56
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pax using the Feature Mapper tool.
At embryonic stage 15, Pax transcript is expressed at a high level in ventral acute muscle 2, at a median level in A1-7 dorsal transverse muscle 1, and at low levels in the segment border muscle, dorsal acute muscle 1, and ventral transverse muscle 1.
Northern analysis using a probe specific to long (~2.4 kB) isoforms are expressed from early embryogenesis with expression peaks at embryonic stage 17--first larval instar and in pharate adults. A probe spefic to only Pax-RD (1.4 kB) is expressed faintly at 8-16 hr AEL and at the third larval instar, at a moderate level in male and female adults, very strongly from 16 hr AEL through the second larval instar and in pharate adults, and not expressed 0-8 hr AEL or in prepupae.
The Pax protein is enriched at the muscle-tendon junction in third instar larvae and is found primarily in muscle cell in the region near the membrane.
In cultured NIH-3T3 cells, fluorescently-tagged Pax-PD short isoform protein localizes along actin bundles, while the long isoform is localized to focal adhesions. In the embyronic/larval muscle system of late embryos, the Pax long isoform is localized to muscle tendon junctions, while the short isoform has particularly strong staining along segment boundaries in the somatic muscle system.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pax in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Annotations CG18061, CG18576 merged as CG31794 in release 3 of the genome annotation.
Source for merge of Paxillin CG18576 was sequence comparison ( date:001128 ).
Source for merge of Paxillin CG18061 was sequence comparison ( date:010307 ).
dsRNA has been made from templates generated with primers directed against this gene.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.