DSRF, SRF, pruned, D-SRF, Drosophila Serum Response Factor
transcription factor - MADS-box motif - required for vein/intervein formation in the fly wing - implicated in longer forms of memory formation like synaptic long-term potentiation and depression - required for a working memory that lasts only for a few seconds - acts as a boosting mechanism to sustain FGF-induced terminal branching in the tracheal system.
Gene model reviewed during 5.42
Gene model reviewed during 5.52
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bs using the Feature Mapper tool.
bs transcripts are expressed ubiquitously in unfertilized eggs. The RNA fades away during germ band extension and zygotic expression is not detected until germ band retraction. At stage 14, expression is observed just beneath the epidermis in a cluster of 6-9 cells in the most anterior portion of each hemisegment from T2 to A8. Expression is also observed in the head, in two rows of cells along the midgut and in a single cell in each hemisegment of the CNS. Other sites include 1 to 2 cells in the dorsal part of each hemisegment from T to A6, cells along the pharyngeal muscle and along the hindgut. In late embryos, weaker expression is observed in somatic muscles.
bs expression was compared in the wing and haltere discs. Expression is present in the wing pouch but not in the haltere.
bs protein is expressed in the wing cell (intervein) region of the wing disc; expression is excluded from the presumptive wing veins and presumptive wing margin.
Well defined staining of crossveins is observed by 23-26 hr APF.
bs protein is expressed ubiquitously in unfertilized eggs. The protein fades away during germ band extension and zygotic expression is not detected until germ band retraction. At stage 14, expression is observed just beneath the epidermis in a cluster of 6-9 cells in the most anterior portion of each hemisegment from T2 to A8. Expression is also observed in the head, in two rows of cells along the midgut and in a single cell in each hemisegment of the CNS. Other sites include 1 to 2 cells in the dorsal part of each hemisegment from T2 to A6, cells along the pharyngeal muscle and along the hindgut. In late embryos, weaker expression is observed in somatic muscles. Using double staining techniques, it was shown that all of the cells that express bs strongly are tightly associated with the tracheal system.
GBrowse - Visual display of RNA-Seq signalsView Dmel\bs in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: bs CG3411
May be part of a pseudoallelic complex with Px.
bs encodes for two protein isoforms, one much longer than the other (449 vs. 355 amino acids). The long isoform is encoded by two transcripts (variants A and C), that use two different transcription start sites and differ only in the first exon. The other protein isoform is encoded by a shorter two-exon transcript (variant B), which shares its TSS with isoform C. The two long isoforms contain a 26 Kb intron, which holds the natural antisense transcript aslncRNA:bsAS.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
bs is involved in cell fate allocation and differentiation but does not participate in cell proliferation. The activity of bs is necessary to specify cells to adopt an intervein fate. It expression is repressed in larval stages in vein territories by genes of the Ras signalling pathway. The differentiation of vein and intervein depends on the outcome of a balance between bs and rho activities, achieved during pupal stages.
bs regulates formation of the terminal branches of the tracheal system. bs may function like mammalian SRF in an inducible transcription complex. Activation of the complex by signals from target tissues induces expression of genes involved in cytoplasmic outgrowth.
The bs gene product plays a dual role during wing differentiation. It acts in a dosage-dependent manner to suppress the formation of wing veins and is required cell-autonomously to promote the development of intervein cells. bs acts at a key step between regulatory genes that define the early positional values in the developing wing disc and the subsequent localized expression of intervein-specific structural genes.
Required for the formation and outgrowth of the terminal branches of the tracheal system.
The bs gene might play a role in the proper formation and maintenance of the trachea.
Developmental and genetic analyses suggest that the bs product is required during metamorphosis for the initiation of intervein development and the concommitant inhibition of vein development.
Bridges, 16th Nov. 1911.