FlyBase Gene Report: Dmel\mew

General Information

Symbol

Dmel\mew

Species

D. melanogaster

Name

multiple edematous wings

Annotation Symbol

CG1771

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004456

Gene Model Status

Current

Stock Availability

30 publicly available

Gene Summary

multiple edematous wings (mew) encodes one of 5 fly integrin alpha subunits, and when in a heterodimer with an integrin beta subunit makes a receptor for the extracellular matrix protein laminin. It is required in numerous tissues for cell migration, adhesion between cell layers, and cell differentiation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

αPS1, α_PS1, PS1, PS1α, αPS1 integrin

Key Links

Genomic Location

Cytogenetic map

11E3-11E8

Sequence location

X:13,214,409..13,258,490 [-]

Recombination map

1-42

RefSeq locus

NC_004354 REGION:13214409..13258490

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (31 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables cell adhesion molecule binding

inferred from mutant phenotype

(Roote and Zusman, 1995)

enables extracellular matrix binding

inferred from direct assay

(Gotwals et al., 1994)

enables protein heterodimerization activity

inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657

(Gotwals et al., 1994)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables signaling receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001144284

(GO Reference Genome Project, 2011-)

Biological Process (20 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from mutant phenotype

(Delon and Brown, 2009)

involved_in axon guidance

inferred from mutant phenotype

(Stevens and Jacobs, 2002)

involved_in axonal defasciculation

inferred from mutant phenotype

(Huang et al., 2007)

involved_in cell adhesion mediated by integrin

inferred from direct assay

(Gotwals et al., 1994)

inferred from mutant phenotype

(Brabant et al., 1996)

involved_in cell adhesion

inferred from mutant phenotype

(Roote and Zusman, 1995)

involved_in imaginal disc-derived wing morphogenesis

inferred from expression pattern

(Brabant et al., 1996)

inferred from mutant phenotype

(Brabant et al., 1996)

involved_in maintenance of epithelial integrity, open tracheal system

inferred from genetic interaction with FLYBASE:if; FB:FBgn0001250

(Levi et al., 2006)

involved_in salivary gland boundary specification

inferred from mutant phenotype

(Kolesnikov and Beckendorf, 2005)

involved_in salivary gland morphogenesis

inferred from genetic interaction with FLYBASE:βTub85D; FB:FBgn0003889

(Jattani et al., 2009)

involved_in somatic muscle attachment to chitin-based cuticle

inferred from genetic interaction with FLYBASE:Grip; FB:FBgn0029830

(Estrada et al., 2007)

inferred from genetic interaction with FLYBASE:kon; FB:FBgn0032683

(Estrada et al., 2007)

involved_in substrate adhesion-dependent cell spreading

inferred from direct assay

(Gotwals et al., 1994)

Terms Based on Predictions or Assertions (11 terms)

CV Term

Evidence

References

involved_in apposition of dorsal and ventral imaginal disc-derived wing surfaces

non-traceable author statement

(Brower, 2003)

involved_in cell adhesion

inferred from electronic annotation with InterPro:IPR000413

(InterPro Project Members, 2004-)

involved_in cell differentiation

traceable author statement

(Brown, 2000)

involved_in cell migration

traceable author statement

(Brown, 2000)

involved_in cell-cell adhesion

traceable author statement

(Brown, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN001144284

(GO Reference Genome Project, 2011-)

involved_in ectodermal digestive tract morphogenesis

traceable author statement

(Brown, 2000)

involved_in heterophilic cell-cell adhesion

traceable author statement

(Hynes and Zhao, 2000)

involved_in imaginal disc-derived wing morphogenesis

traceable author statement

(Brown, 2000)

involved_in integrin-mediated signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001144284

(GO Reference Genome Project, 2011-)

involved_in midgut development

traceable author statement

(Brown, 2000)

involved_in salivary gland development

traceable author statement

(Abrams et al., 2003)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Dinkins et al., 2008)

located_in basal plasma membrane

inferred from direct assay

(Brabant et al., 1996)

located_in focal adhesion

inferred from direct assay

(Delon and Brown, 2009)

part_of integrin complex

inferred from direct assay

(Gotwals et al., 1994)

located_in lateral plasma membrane

inferred from direct assay

(Dinkins et al., 2008)

located_in plasma membrane

inferred from direct assay

(Gotwals et al., 1994)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cell surface

inferred from biological aspect of ancestor with PANTHER:PTN001144284

(GO Reference Genome Project, 2011-)

part_of integrin complex

inferred from electronic annotation with InterPro:IPR000413

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN001144284

(GO Reference Genome Project, 2011-)

non-traceable author statement

(Brower, 2003)

inferred from sequence or structural similarity with UniProtKB:P26006

(Brown, 2000)

Gene Group (FlyBase)

INTEGRINS

Protein Family (UniProt)

Belongs to the integrin alpha chain family. (Q24247)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

INTEGRINS -: Integrins are heterodimeric transmembrane receptors composed of an α and β subunit that mediate cell-cell and cell-extracellular matrix adhesion. As well as maintaining tissue integrity, they are involved in morphogenesis and development. (Adapted from FBrf0167428).

Protein Function (UniProtKB)

Integrin alpha-PS1/beta-PS is a receptor for laminin.

(UniProt, Q24247)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mew for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24247)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Stop-codon suppression (UAA) postulated; FBrf0216884.

Gene model reviewed during 5.43

Shares 5' UTR with upstream gene.

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mew-RA

FBtr0073731

NM_167354

3863

1049

mew-RB

FBtr0073732

NM_078590

5001

1146

mew-RC

FBtr0310647

NM_001272575

4281

1155

mew-RD

FBtr0310648

NM_001272576

4350

1058

mew-RE

FBtr0330398

NM_001272574

3863

1146

mew-RF

FBtr0340190

NM_001298267

4254

1243

Additional Transcript Data and Comments

Reported size (kB)

5.4 (northern blot)

(Wehrli et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mew-PA

FBpp0073562

117.1

1049

5.77

NP_727679

AAN09652

mew-PB

FBpp0073563

128.0

1146

6.27

FBpp0302767

129.0

1155

6.31

NP_001259504

AGB95346

mew-PD

FBpp0302768

118.1

1058

5.84

NP_001259505

AGB95347

mew-PE

FBpp0303424

128.3

1146

6.46

NP_001259503

AGB95345

mew-PF

FBpp0309167

139.2

1243

6.77

X2JDJ5

NP_001285196

AHN59666

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1146 (aa); 128 (kD predicted)

(Wehrli et al., 1993)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-PS1 associates with beta-PS.

(UniProt, Q24247)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mew using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mew_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.33

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 5 -- 6

dorsal ectoderm anlage

(Sawala et al., 2015)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

clypeo-labral primordium

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 13

longitudinal trunk visceral muscle primordium

(Urbano et al., 2011)

embryonic stage 12

tracheal pit

(Bradley et al., 2003)

radioisotope in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval posterior midgut

(Wehrli et al., 1993)

presumptive embryonic/larval central nervous system

(Wehrli et al., 1993)

ectoderm

(Wehrli et al., 1993)

embryonic/larval anterior midgut

(Wehrli et al., 1993)

third instar larval stage

adepithelial cell of wing disc

(Wehrli et al., 1993)

wing disc

(Wehrli et al., 1993)

peripodial epithelium

(Wehrli et al., 1993)

Additional Descriptive Data

Transcript was detected in stage 11 salivary gland primordia coincident with salivary gland internalization. Expression continued in all cells through stage 12 with high expression in invaginating cells. Transcritp was no longer detectable at stage 14. mew is also expressed in the invaginating tracheal pits.

(Bradley et al., 2003)

mew transcripts are first detected at the cellular blastoderm stage in the cells of the presumptive ectoderm. During gastrulation, the transcript is apparent over the entire ectoderm with increased levels in the dorsal region. In stage 8, high transcript levels are observed in the ectoderm and in the anterior and posterior midgut primordia. By stage 11, a strong segmentally repeated pattern has been established in the ectoderm and the CNS. The regions of high concentration are correlated with segment boundaries. Strong mew expression is also detected in the dorsal compartment of the wing disc as well as in adepithelial cells and on the peripodial membrane.

(Wehrli et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

embryonic cardioblast | apical

(Vanderploeg et al., 2012)

third instar larval stage

eye disc anterior to the morphogenetic furrow

(Fernandes et al., 2014)

eye disc morphogenetic furrow

(Fernandes et al., 2014)

pupal stage P6

wing cell | basal

(Negreiros et al., 2010)

adult stage

intestinal stem cell | basal

(Lin et al., 2013)

enteroblast | basal

(Lin et al., 2013)

oogenesis stage S9 -- S11

dorsal appendage forming follicle cell

(Dinkins et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

The α1 integrin, mew, strongly accumulates on the basal membrane of esg-positive cells contacting ECM, but is barely detected in any other regions along the interface between the muscle cells and the differentiated epithelial cells.

(Lin et al., 2013)

In stage 15 embryos, before the leading processes of the cardioblasts make contact across the midline, mew is localised primarily on the leading edge of the migrating cardioblasts, and less so on the trailing edge. This preponderantly apical distribution was not significantly altered once the heart lumen was formed at stage 17.

(Vanderploeg et al., 2012)

mew staining is observed along lateral, apical, and basal follicle cell membranes through stage 10A. Apical staining peaks in stage 9 and 10A columnal follicle cells overlying the oocyte while downregulating in the most posterior follicle cells. Expression is also seen in border cells at intercellular junctions. After stage 10A, the remaining follicle cell populations begin down-regulating apically localized mys while maintaining lateral and basal localization during dorsal appendage morphogenesis. Staining is observed from stage 9 in dorsal appendage forming follicle cells.

(Dinkins et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Dinkins et al., 2008)

located_in basal plasma membrane

inferred from direct assay

(Brabant et al., 1996)

located_in focal adhesion

inferred from direct assay

(Delon and Brown, 2009)

part_of integrin complex

inferred from direct assay

(Gotwals et al., 1994)

located_in lateral plasma membrane

inferred from direct assay

(Dinkins et al., 2008)

located_in plasma membrane

inferred from direct assay

(Gotwals et al., 1994)

Expression Deduced from Reporters

Reporter: PBac{681.P.FSVS-1}mew^CPTI001678

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mew in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mew

View exonic expression by tissue for Dmel\mew

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004456

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32275/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004456

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004456

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004456

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1771

Flygut - An atlas of the Drosophila adult midgut

FBgn0004456

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\mew

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 36 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mew

Transgenic constructs containing regulatory region of mew

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(1)N12

(Walsh and Brown, 1998, Brower et al., 1995)

In(1)mew^M6

(Brower et al., 1995)

Gene duplicated in

Dp(1;3)DC266

(Popodi et al., 2010-, Venken et al., 2010)

Dp(1;f)y⁺wy⁺

(Brower et al., 1995)

Inferred from location ( 19 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

lethal - all die before end of larval stage

mew^G0429

lethal - all die before end of larval stage | recessive

partially lethal - majority die | female

mew^G0443

partially lethal - majority die | male

mew^G0443

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mew^GD1230

viable, with Scer\GAL4^Mef2.PR

mew^GD1230

viable, with Scer\GAL4^pnr-MD237

mew^GD1230

viable, with Scer\GAL4^tin.CΔ4

mew^GD1230

Sterility

Allele

fertile

mew^EY09631

Other Phenotypes

Allele

abnormal neuroanatomy

mew^M6

abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4^109(2)80

mew^M6

abnormal size | adult stage | somatic clone

decreased cell number | adult stage | somatic clone

short lived, with Scer\GAL4^Ugt36A1.PK

some die during larval stage

mew^G0429

some die during larval stage | recessive

visible | recessive | heat sensitive

mew^A1-5

Phenotype manifest in

Allele

adult pericardial cell, with Scer\GAL4^Ugt36A1.PK

adult posterior midgut epithelium | somatic clone

anterior midgut primordium

dorsal tracheal branch primordium, with Scer\GAL4^btl.PS

mew^UAS.cWa

embryonic/larval midgut

embryonic/larval midgut | embryonic stage

mew^M6

embryonic/larval salivary gland

mew^M6

embryonic/larval visceral tracheal branch

mew^M6

embryonic midgut constriction

fascicle

mew^M6

filamentous actin | somatic clone

mew^M6

follicle cell | somatic clone

mew^M6

larval dorsal multidendritic neuron ddaC | somatic clone | third instar larval stage, with Scer\GAL4^109(2)80

mew^M6

larval intersegmental nerve

mew^M6

larval segmental nerve

mew^M6

mitotic domain 1 | extended germ band stage

muscle attachment site | embryonic stage

mew^M6

oocyte | oogenesis stage S14

mew⁴⁹⁸

photoreceptor

mew⁴⁹⁸

presumptive embryonic/larval central nervous system

presumptive embryonic/larval digestive system

mew^M6

presumptive embryonic/larval peripheral nervous system

presumptive embryonic salivary gland

mew^M6

retina

mew⁸¹

retina | somatic clone

salivary gland common duct primordium

wing

wing | heat sensitive

mew^A1-5

wing | somatic clone

wing blade dorsal compartment | somatic clone

mew⁸¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\ITGA6

13 of 14

Yes

Hsap\ITGA3

12 of 14

Yes

Hsap\ITGA7

12 of 14

Yes

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (19)

Rnor\Itga7

13 of 14

Yes

Rnor\Itga3

12 of 14

Yes

Rnor\Itga6

12 of 14

Yes

Rnor\Itga2b

5 of 14

Rnor\Itga8

4 of 14

Rnor\Itgae

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (19)

Mmus\Itga3

13 of 14

Yes

Mmus\Itga7

13 of 14

Yes

Mmus\Itga6

12 of 14

Yes

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Xenopus tropicalis (Western clawed frog) (27)

Xtro\itga6

11 of 13

Yes

Xtro\itga3

10 of 13

Yes

Xtro\itga7

6 of 13

Yes

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

Yes

Xtro\dnajc4

1 of 13

Xtro\haus2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\itga11

1 of 13

Xtro\itgad

1 of 13

Xtro\itgae

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (25)

Drer\itga3a

13 of 14

Yes

Drer\itga3b

13 of 14

Yes

Drer\itga6a

13 of 14

Yes

Drer\itga6b

12 of 14

Yes

Drer\itga6l

11 of 14

Yes

Drer\itga2b

4 of 14

Drer\itgae.1

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Drer\itgam.2

1 of 14

Yes

Drer\LOC101882757

1 of 14

Yes

Drer\LOC110438995

1 of 14

Yes

Drer\LOC110439202

1 of 14

Yes

Drer\zmp:0000001082

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\ina-1

14 of 14

Yes

Cele\pat-2

4 of 14

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP000307

12 of 12

Yes

Agam\AgaP_AGAP000032

3 of 12

Agam\AgaP_AGAP004303

3 of 12

Agam\AgaP_AGAP006826

2 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mew. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (4)

5 of 13

ItgaPS4

5 of 13

scb

5 of 13

ItgaPS5

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ITGA7; integrin subunit alpha 7

model of congenital muscular dystrophy due to integrin alpha-7 deficiency

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

mew^JF02694

ameliorates intestinal cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Bajpai et al., 2020)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Hernandez et al., 2023)

mew^HMS02849

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Hernandez et al., 2023)

mew^TKO.GS00808

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Hernandez et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ITGA6; integrin subunit alpha 6

13 of 14

147556

EPIDERMOLYSIS BULLOSA, JUNCTIONAL 6, WITH PYLORIC ATRESIA; JEB6

ITGA3; integrin subunit alpha 3

12 of 14

605025

EPIDERMOLYSIS BULLOSA, JUNCTIONAL 7, WITH INTERSTITIAL LUNG DISEASE AND NEPHROTIC SYNDROME; JEB7

ITGA7; integrin subunit alpha 7

12 of 14

600536

MUSCULAR DYSTROPHY, CONGENITAL, DUE TO INTEGRIN ALPHA-7 DEFICIENCY

congenital muscular dystrophy due to integrin alpha-7 deficiency

ITGA2B; integrin subunit alpha 2b

5 of 14

607759

ITGAV; integrin subunit alpha V

5 of 14

193210

IMMUNE DYSREGULATION, NEURODEVELOPMENTAL DEFECTS, AND COLITIS; IDNDC

ITGA5; integrin subunit alpha 5

4 of 14

135620

ITGA8; integrin subunit alpha 8

4 of 14

604063

RENAL HYPODYSPLASIA/APLASIA 1; RHDA1

renal agenesis

ITGAE; integrin subunit alpha E

4 of 14

604682

ITGA1; integrin subunit alpha 1

3 of 14

192968

ITGA10; integrin subunit alpha 10

3 of 14

604042

ITGAD; integrin subunit alpha D

3 of 14

602453

ITGAL; integrin subunit alpha L

3 of 14

153370

ITGAM; integrin subunit alpha M

3 of 14

120980

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mew - kon

bead aggregation assay, fluorescence microscopy, inferred by author

(Pérez-Moreno et al., 2017)

mew - mys

anti bait coimmunoprecipitation, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Orr et al., 2022, Gotwals et al., 1994)

mew - rhea

anti tag coimmunoprecipitation, anti tag western blot

(Orr et al., 2022)

mew - sog

anti bait coimmunoprecipitation, western blot

(Araujo et al., 2003)

mew - tkv

anti bait coimmunoprecipitation, anti tag western blot

(Sawala et al., 2015)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mew

enhanceable

blo

(Walsh and Brown, 1998)

mew

enhanceable

(Walsh and Brown, 1998)

mew

suppressible

if::mew

(Martin-Bermudo et al., 1997)

mew

suppressible

(Li et al., 1998, Martin-Bermudo et al., 1997, Roote and Zusman, 1996)

mew

enhanceable

jbug

(Walsh and Brown, 1998)

mew

enhanceable

pot

(Walsh and Brown, 1998)

mew

enhanceable

sli

(Stevens and Jacobs, 2002)

mew

enhanceable

Tsp

(Chanana et al., 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

14-3-3ε

suppressible

mew

(Yang and Terman, 2012)

suppressible

fng|mew

(O'Keefe and Thomas, 2001)

fne

suppressible

mew|mys

(Alizzi et al., 2020)

fra

enhanceable

mew

(Kolesnikov and Beckendorf, 2005)

fry

suppressible

mew|mys

(Han et al., 2012)

suppressible

mew

(Müller et al., 2005)

enhanceable

mew

(Prokop et al., 1998)

suppressible

mew

(Martin-Bermudo et al., 1997)

suppressible

(Han et al., 2012)

enhanceable

(Chountala et al., 2012)

suppressible

(Lee et al., 2015)

suppressible

(Soba et al., 2015)

Sema2b

suppressible

mew|mys

(Meltzer et al., 2016)

suppressible

(Han et al., 2012)

enhanceable

(Stevens and Jacobs, 2002)

sog

suppressible

mew

(Araujo et al., 2003)

Tsp

enhanceable

mew

(Chanana et al., 2007)

yki

suppressible

mew

(Bajpai et al., 2020)

External Data

Subunit Structure (UniProtKB)

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-PS1 associates with beta-PS.

(UniProt, Q24247 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58660

DroID - A comprehensive database of gene and protein interactions.

FBgn0004456

MIST (genetic) - An integrated Molecular Interaction Database

32275

MIST (protein-protein) - An integrated Molecular Interaction Database

32275

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-42

Cytogenetic map

11E3-11E8

Sequence location

X:13,214,409..13,258,490 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11E3-11E8

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

11E-11F

(determined by in situ hybridisation) 11E1--4 (determined by in situ hybridisation) 11D1--2 (determined by in situ hybridisation)

(Peter et al., 2002)

11E-11F

(determined by in situ hybridisation)

(Schäfer et al., 1999.12.2)

11E1-11E4

(determined by in situ hybridisation)

(Schäfer et al., 1999.8.12)

11D7-11E4

(determined by in situ hybridisation)

(Wehrli and Wilcox, 1990)

11D7-11E5

(determined by in situ hybridisation)

(Wehrli et al., 1993)

Experimentally Determined Recombination Data

Location

1-42

(FlyBase, 2016)

1-42.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (30)

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (22)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH14509

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004456

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004456

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Gullberg et al., 1994)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

DK.1A4

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

(McDonald and Rosbash, 2001)

mew and scb are required in a partially redundant way in the endoderm for normal migration of the endodermal midgut cells over the visceral mesoderm in the developing embryo.

(Martin-Bermudo et al., 1999)

The functional significance of the cytoplasmic domains of the if, mew and mys subunits of the Position Specific (PS) integrin family are studied by analysing the relationship between the cytoplasmic domain structure and function in the context of a developing organism. The cytoplasmic tail of mew is not required for function in the wing and in the retina.

(Li et al., 1998)

mew can mediate adhesion of the epidermal hemiadherens junctions to the basement membrane at muscle attachment sites in the developing embryo. This function appears to be mediated by LanA.

(Prokop et al., 1998)

The ability of two different integrin α subunits (encoded by mew and if) to substitute for each other during embryonic development has been studied. The two α subunits encoded by mew and if are not equivalent and have distinct functions which reside in the extracellular domains.

(Martin-Bermudo et al., 1997)

Clonal analysis demonstrates that the different integrins, mew and if, are required on opposite wing epithelia. An early integrin-dependent process, not obviously required for prepupal adhesion, is essential to permit subsequent wing morphogenesis.

(Brabant et al., 1996)

Loss of mew produces disorganisation along the basal surface of the retina. if function can replace mew function in the retina.

(Roote and Zusman, 1996)

Phenotypic analysis of mew, if and mys embryos suggests multiple roles for PS integrins in the adhesion of cells and in the formation, organization and migration of embryonic tissues. Although the proteins are often expressed in adjacent embryonic tissues, this distribution does not necessarily reflect equivalent requirements. The complete loss of both α subunits, encoded by mew and if, does not produce all the phenotypes observed in embryos lacking the mys encoded β subunit.

(Roote and Zusman, 1995)

mew gene product promotes cell spreading on two different extra cellular matrix (ECM) molecules, laminin and tiggin.

(Gotwals et al., 1994)

Relationship to Other Genes

Source for database merge of

Source for merge of: mew l(1)G0443 l(1)G0429

(Peter et al., 2002)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: mew CG1771

(Gong, 1999.11)

Nomenclature comments

Etymology

The chosen gene symbol, "mew", will commemorate the name of the late Michael E. Wilcox, who made many important contributions to this field.

(Brower et al., 1995)

Synonyms and Secondary IDs (39)

Reported As

Symbol Synonym

CG1771

(Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Schiess et al., 2009, Bourbon et al., 2002, Whitfield, 2001.1.5)

CT5254

(Hynes and Zhao, 2000)

Integrin alpha PS1

(Kim et al., 2018)

ItgaPS1

(Brown et al., 2013.2.5)

Mew

(Walther et al., 2024, Titlow et al., 2023, Fu et al., 2017, Copf, 2015)

PS1

(Tomoyasu et al., 2009, Estrada et al., 2007, MacMullin and Jacobs, 2005, Solnica-Krezel and Eaton, 2003, Genova et al., 2000, Hobert and Westphal, 2000, Martin-Bermudo and Brown, 1999, Brabant et al., 1996, Fernandes et al., 1996, Cohen, 1993, Williams and Carroll, 1993, Peel and Milner, 1992)

PS1α

(Roy and VijayRaghavan, 1999, Madueno et al., 1995, Wessendorf et al., 1992, Hirano et al., 1991)

PSα1

(Delanoue et al., 2002)

aPS1

(Gonçalves et al., 2025, Hoang and Chiba, 1998, Prokop et al., 1997)

alpha-PS1

(Wehrli, 1993.7.8)

alphaPS1

(Bouge et al., 2005)

l(1)G0429

(Schäfer et al., 1999.8.12)

l(1)G0443

(Schäfer et al., 1999.12.2)

mew

(Bischoff et al., 2025, Luedke et al., 2024, Sun et al., 2024, Yamamoto et al., 2024, Forbes Beadle et al., 2023, Hernandez et al., 2023, Min et al., 2023, Harrison et al., 2021, Pang et al., 2021, Pitsidianaki et al., 2021, Bajpai et al., 2020, Hung et al., 2020, Pastor-Pareja, 2020, Arbel et al., 2019, Deshpande et al., 2019, Jiang et al., 2019, Meltzer et al., 2019, Thuveson et al., 2019, Grobler et al., 2018, Kim et al., 2018, Park et al., 2018, Hermle et al., 2017, Kline et al., 2017, Pérez-Moreno et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Loganathan et al., 2016, Meltzer et al., 2016, Peters and Berg, 2016, Lee et al., 2015, Maartens and Brown, 2015, Myat et al., 2015, Panda et al., 2015, Patel et al., 2015, Sawala et al., 2015, Soba et al., 2015, Van Bortle et al., 2015, Ashwal-Fluss et al., 2014, Jiang et al., 2014, Kim and Choe, 2014, Kim et al., 2014, You et al., 2014, Brown et al., 2013.2.5, Ismat et al., 2013, Lin et al., 2013, Nonaka et al., 2013, Pirraglia et al., 2013, Sen et al., 2013, Terriente-Felix et al., 2013, Thimgan et al., 2013, Weavers and Skaer, 2013, Yamamoto et al., 2013-, Cho et al., 2012, Gates, 2012, Han et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kim et al., 2012, Jungreis et al., 2011, Lott et al., 2011, Negreiros et al., 2010, Popodi et al., 2010-, Tikhmyanova et al., 2010, Venken et al., 2010, Delon and Brown, 2009, Jattani et al., 2009, Krejcí et al., 2009, Schotman et al., 2009, Bai et al., 2008, Chen et al., 2008, Dinkins et al., 2008, O'Reilly et al., 2008, Schotman et al., 2008, Tsai et al., 2008, Zhang et al., 2008, Beltran et al., 2007, Chanana et al., 2007, Conder et al., 2007, Estrada et al., 2007, Huang et al., 2007, Junion et al., 2007, Levi et al., 2006, Curtin et al., 2005, Ayyub and Paranjape, 2002, FlyBase, 1992-)

α integrin

(Sun et al., 2020)

α-PS1

(Aradhya et al., 2015)

α1Int

(Yang and Terman, 2012, Huang et al., 2007)

α_PS1

(Chanana et al., 2007, Bradley et al., 2003, Fujimoto et al., 1999, Martin-Bermudo et al., 1999, Li et al., 1998, Prokop et al., 1998, Lin et al., 1997, Martin-Bermudo et al., 1997, Ashkenas et al., 1996, Blair et al., 1994, Fessler et al., 1994, Gullberg et al., 1994, Brown, 1993, Wehrli et al., 1993)

αPS1

(Hernandez et al., 2023, Park et al., 2018, Pérez-Moreno et al., 2017, Valdivia et al., 2017, Ashe, 2016, Peters and Berg, 2016, Sawala et al., 2015, Xie et al., 2014, Ismat et al., 2013, Lin et al., 2013, Pirraglia et al., 2013, Bulgakova et al., 2012, Chountala et al., 2012, Gates, 2012, Egoz-Matia et al., 2011, Urbano et al., 2011, Xie and Auld, 2011, Delon and Brown, 2009, Jattani et al., 2009, Krejcí et al., 2009, Chen et al., 2008, Dinkins et al., 2008, O'Reilly et al., 2008, Schotman et al., 2008, Fernández-Miñán et al., 2007, Subramanian et al., 2007, Takada et al., 2007, Tanentzapf et al., 2007, Lo, 2006, MacMullin and Jacobs, 2006, Curtin et al., 2005, Abrams et al., 2003, Brower, 2003, Schock and Perrimon, 2003, Baker et al., 2002, Bokel and Brown, 2002, Stevens and Jacobs, 2002, Araujo et al., 2001, Hughes, 2001, O'Keefe and Thomas, 2001, Brown, 2000, Brown et al., 2000, D'Avino and Thummel, 2000, Hynes and Zhao, 2000, Kim and Chiba, 2000, Martin-Bermudo, 2000, Beumer et al., 1999, Hoang and Chiba, 1998, Martin-Bermudo et al., 1997, Yarnitzky and Volk, 1995, Gotwals et al., 1994, Gotwals et al., 1994, Brown et al., 1993)

Name Synonyms

Integrin alpha PS1

(Khadilkar et al., 2020)

Integrin alphaPS1 subunit

(Brown et al., 2013.2.5)

Multiple edematous wings

(Koehler and Huber, 2023, Xie et al., 2014)

PS I alpha

(Kiss et al., 2016)

PS1 integrin

(Uv et al., 2003, Muller, 2000, Martinez Arias, 1993)

PS1-Integrin

(Peters and Berg, 2016)

Position-specific-1 antigen

(Fristrom and Fristrom, 1993)

Position-specific-antigen-1α

integrin

(Bittern et al., 2021)

integrin PSII alpha subunit

(Kiss et al., 2016)

integrin αPS1

(Schotman et al., 2009)

integrin αPS1 subunit

(Brown et al., 2013.2.5)

multiple edematous wings

(Sawala et al., 2015)

multiple edematous wing

(Pérez-Moreno et al., 2017, Ayyub and Paranjape, 2002)

multiple edematous wings

α integrin

(Fujimoto et al., 1997)

α-PS1-integrin

(Kohsaka et al., 2007, Goode et al., 1996)

α1 Integrin subunit

(Pasco et al., 2015)

α1 integrin

(Huang et al., 2007)

αPS1 subunit of integrin

(Chanana et al., 2007)

αPS integrin

(Kiger et al., 2007)

αPS1

(Negreiros et al., 2010)

αPS1 integrin

(Weavers and Skaer, 2013, Zhang and Ten Hagen, 2011, Jattani et al., 2009, Lo, 2006, Ribeiro et al., 2003)

Secondary FlyBase IDs

FBgn0028276
FBgn0040161

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 70 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32275

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24247

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24247

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JDJ5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24247

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004456

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32275/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004456

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004456

FlyMine - An integrated database for Drosophila genomics

FBgn0004456

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1771

MARRVEL_MODEL - MARRVEL (model organism gene)

32275

Linkouts

BioGRID - A database of protein and genetic interactions.

58660

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004456

DroID - A comprehensive database of gene and protein interactions.

FBgn0004456

DRSC - Results frm RNAi screens

FBgn0004456

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

DK.1A4

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mew_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004456

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004456

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1771

Flygut - An atlas of the Drosophila adult midgut

FBgn0004456

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004456

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034177

MIST (genetic) - An integrated Molecular Interaction Database

32275

MIST (protein-protein) - An integrated Molecular Interaction Database

32275

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (301)

The following external sites may use different assemblies or annotations than FlyBase.

-1.33

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