FlyBase Allele Report: Dmel\mew[M6]

Slight irregularities in the apical surface of the salivary gland are seen at stage 12 in mutant embryos. By stage 14/15 the salivary gland tube is buckled.

(Ismat et al., 2013)

Adult mew^M6 homozygous mutant intestinal stem cell clones have reduced maintenance 7 days and 14 days after clone induction compared to control clones; remaining clones contain fewer cells by day 14; enteroendocrine cell/enterocyte ratio is unaffected and intestinal stem cells show no obvious signs of apoptosis.

(Lin et al., 2013)

mew^M6 mutant class IV da neurons (generated using the MARCM system under the control of Scer\GAL4^109(2)80) have significantly more dendrites that are enclosed in the epidermis rather than attached to the ECM in comparison with wild-type. They also exhibit an increased number of dendritic crossings, and 86.97% of these are non-contacting as the dendrites are in a different apical-basal plane.

(Han et al., 2012)

mew^M6 follicle cell clones organize stress fibres similar to wild type, but have elevated levels of basal F-actin. These cells do not display cell shape defects.

(Delon and Brown, 2009)

Hemizygous mew^M6/Y mutant embryos display muscle detachments in only few segments.

(Chanana et al., 2007)

Homozygous embryos show axon guidance defects characteristic of increased fasciculation in the ISNb pathway (82.3% of hemisegments), in the SNa pathway (71.8% of hemisegments) and in the central nervous system (55.8% of hemisegments).

(Huang et al., 2007)

mew^M6 dorsal branch terminal cell clones do not cause any detectable defects in terminal cell morphology.

(Levi et al., 2006)

mew^M6 mutant embryos show salivary gland guidance defects. A minority of these mutants have salivary glands that lie parallel to the CNS midline, as in wild type. 35% of these mew^M6 embryos possess at least one gland that curves medially towards the midline, while a further 35% have at least one gland that curves laterally away from the midline.

(Kolesnikov and Beckendorf, 2005)

Salivary cells invaginate and move dorsally to the visceral mesoderm in mutant embryos, but fail to migrate away from the turning point (the point where the cells turn to reorient their movement posteriorly in wild-type embryos). The salivary glands are often bent and mispositioned in late stage mutant embryos. The length and diameter of the mutant salivary glands is similar to wild type.

(Bradley et al., 2003)

mew^M6 mutant embryos exhibit thinning of the longitudinal fascicles (visible with Fas2), with occasional defasciculation defects.

(Stevens and Jacobs, 2002)

In mutant embryos, the visceral branches of the tracheal system are shorter than wild-type, though fine branches still exist. During development, they reach and contact the visceral mesoderm, but do not migrate along the mesoderm.

(Boube et al., 2001)

The continuous layer of visceral mesoderm that normally surrounds the gut is moderately disrupted in homozygous embryos.

(Martin-Bermudo and Brown, 1999)

Endodermal midgut cells of homozygous embryos show a modest delay of approximately 30 minutes in midgut migration, so that anterior and posterior midgut cells do not contact each other until stage 13 when wild-type cells have already fused. The endodermal cells still send projections towards the visceral mesoderm, as in wild type. The arrangement of the visceral mesoderm cells is normal in homozygous embryos.

(Martin-Bermudo et al., 1999)

Homozygous embryos have an increased rate of axon errors in the central and peripheral nervous systems compared to wild-type.

(Hoang and Chiba, 1998)

Homozygous embryos do not have any ultrastructural defects at muscle attachment sites.

(Prokop et al., 1998)

Most homozygous larvae die during the first or second instar stages.

(Martin-Bermudo et al., 1997)

In 30 and 36 hour after pupariation (AP) dorsal clones are associated with pronounced matrix-filled blisters. Small clones (fewer than 150 cells) in the wing are often wild type or have a weak phenotype, even if on the dorsal surface.

(Brabant et al., 1996)

Mitotic clones in the eye exhibit photoreceptor disorganisation and holes resulting in spaces between and within ommatidial units. Disruption is mild and defects are often restricted to the basal surface of the retina.

(Roote and Zusman, 1996)

Midgut does not elongate into the tubular structure but there are no obvious defects in somatic muscle morphogenesis. Dorsal herniation is not observed in homozygotes or double mutant combinations with if^k27e and if^B4. Large dorsal wing clones cause wing blisters, small clones of approximately 5 cells have no phenotype and clones of 100 cells show a mild phenotype such as wrinkles confined to one end of the clone. Large dorsal wing clones cause wing blisters, small clones of approximately 5 cells have no phenotype and clones of 100 cells show a mild phenotype such as wrinkles confined to one end of the clone. Clones in the eye exhibit photoreceptor disorganisation.

(Brower et al., 1995)

Clonal analysis reveals that lack of mew has no discernible effect on germ cell development. Mutant embryos show a distinct and reproducible separation between the ectodermal and mesodermal tissue layers of the germband, though the separation is not complete. Midgut primordia meet and initiate fusion at least 1 to 2 hours after wild type embryos would. Visceral mesoderm appears normal. Midgut constrictions partially form.

(Roote and Zusman, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mew^M6 has abnormal neuroanatomy phenotype, enhanceable by sli²

(Stevens and Jacobs, 2002)

mew^A1-5/mew^M6 has visible phenotype, enhanceable by pot[+]/pot^P3

(Walsh and Brown, 1998)

Suppressed by

Statement

Reference

mew^M6 has lethal phenotype, suppressible by if^m8.UAS/Scer\GAL4^48Y

(Martin-Bermudo et al., 1997)

mew^M6 has lethal phenotype, suppressible by Scer\GAL4^48Y/if^C.UAS

(Martin-Bermudo et al., 1997)

mew^M6 has lethal phenotype, suppressible by if::mew^UAS.cMBb/Scer\GAL4^48Y

(Martin-Bermudo et al., 1997)

mew^M6 has lethal phenotype, suppressible by if::mew^UAS.cMBa/Scer\GAL4^48Y

(Martin-Bermudo et al., 1997)

mew^M6 has lethal phenotype, suppressible by Scer\GAL4^48Y/if^UAS.cMBa

(Martin-Bermudo et al., 1997)

Enhancer of

Statement

Reference

mew^M6 is an enhancer of abnormal neuroanatomy phenotype of sli^unspecified

(Stevens and Jacobs, 2002)

Suppressor of

Statement

Reference

mew^M6 is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

mew[+]/mew^M6 is a suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

mew[+]/mew^M6 is a suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Other

Statement

Reference

Ret^C168/Ret[+], mew^M6 has abnormal neuroanatomy phenotype

(Soba et al., 2015)

LanA^9-32, mew[+]/mew^M6 has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

mew[+]/mew^M6, wb⁰⁹⁴³⁷ has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

Scer\GAL4^109(2)80, mew^M6, mys¹/mys[+] has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype

(Han et al., 2012)

LanB2^MB04039, mew[+]/mew^M6 has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

Df(3L)Exel6083/+, mew^M6 has abnormal neuroanatomy | dominant phenotype

(Huang et al., 2007)

mew^M6, mys[+]/mys^olfC-x17 has abnormal smell perception phenotype

(Ayyub and Paranjape, 2002)

mew^M6, mys^olfC-x3/mys[+] has abnormal smell perception phenotype

(Ayyub and Paranjape, 2002)

mew^M6, mys^olfC-x17 has abnormal smell perception phenotype

(Ayyub and Paranjape, 2002)

mew^M6, mys^olfC-x3 has abnormal smell perception phenotype

(Ayyub and Paranjape, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

mew^M6 has muscle attachment site phenotype, enhanceable by Tsp[+]/Tsp^unspecified

(Chanana et al., 2007)

mew^M6 has fascicle phenotype, enhanceable by sli²

(Stevens and Jacobs, 2002)

mew^A1-5/mew^M6 has wing phenotype, enhanceable by pot[+]/pot^P3

(Walsh and Brown, 1998)

NOT Enhanced by

Statement

Reference

mew^M6 has adult posterior midgut epithelium | somatic clone phenotype, non-enhanceable by if^k27e/if^k27e

(Lin et al., 2013)

Suppressed by

Statement

Reference

mew^M6 has phenotype, suppressible by if::mew^UAS.cMBb/Scer\GAL4^48Y

(Martin-Bermudo et al., 1997)

mew^M6 has retina | somatic clone phenotype, suppressible by if^C.UAS/Scer\GAL4^hs.PB

(Roote and Zusman, 1996)

mew^M6 has retina | somatic clone phenotype, suppressible by if^m8.UAS/Scer\GAL4^hs.PB

(Roote and Zusman, 1996)

NOT suppressed by

Statement

Reference

mew^M6 has adult posterior midgut epithelium | somatic clone phenotype, non-suppressible by if^k27e/if^k27e

(Lin et al., 2013)

mew^M6 has embryonic/larval visceral tracheal branch phenotype, non-suppressible by Scer\GAL4^btl.PS/if^UAS.cMBa

(Boube et al., 2001)

mew^M6 has embryonic/larval midgut | embryonic stage phenotype, non-suppressible by Scer\GAL4^48Y/if^UAS.cMBa

(Martin-Bermudo et al., 1999)

mew^M6 has wing | somatic clone phenotype, non-suppressible by Scer\GAL4⁶⁸⁴/if^C.UAS

(Brabant et al., 1996)

Enhancer of

Statement

Reference

mew^M6 is an enhancer of muscle attachment site phenotype of Tsp^Δ79

(Chanana et al., 2007)

mew^M6 is an enhancer of presumptive embryonic salivary gland phenotype of fra³/fra⁴

(Kolesnikov and Beckendorf, 2005)

mew^M6 is an enhancer of fascicle phenotype of sli^unspecified

(Stevens and Jacobs, 2002)

NOT Enhancer of

Statement

Reference

mew[+]/mew^M6 is a non-enhancer of heart primordium phenotype of sli²

(MacMullin and Jacobs, 2006)

Suppressor of

Statement

Reference

mew^M6 is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

mew[+]/mew^M6 is a suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

mew[+]/mew^M6 is a suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

NOT Suppressor of

Statement

Reference

mew[+]/mew^M6 is a non-suppressor of eye phenotype of Atg1^K.GMR

(Chen et al., 2008)

Other

Statement

Reference

Ret^C168/Ret[+], mew^M6 has larval multidendritic class IV neuron phenotype

(Soba et al., 2015)

LanA^9-32, mew[+]/mew^M6 has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Han et al., 2012)

mew[+]/mew^M6, wb⁰⁹⁴³⁷ has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Han et al., 2012)

Scer\GAL4^109(2)80, mew^M6, mys¹/mys[+] has larval dorsal multidendritic neuron ddaC | somatic clone | third instar larval stage phenotype

(Han et al., 2012)

LanB2^MB04039, mew[+]/mew^M6 has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Han et al., 2012)

βTub85Dⁿ, mew^M6 has presumptive embryonic salivary gland phenotype

(Jattani et al., 2009)

Df(3L)Exel6083/+, mew^M6 has larval intersegmental nerve phenotype

(Huang et al., 2007)

Df(3L)Exel6083/+, mew^M6 has presumptive embryonic/larval central nervous system phenotype

(Huang et al., 2007)

Df(3L)Exel6083/+, mew^M6 has larval segmental nerve phenotype

(Huang et al., 2007)

if^k27e, mew^M6 has terminal tracheal cell | somatic clone phenotype

(Levi et al., 2006)

mew^M6, mys^b45 has neuron & eye photoreceptor cell phenotype

(Curtin et al., 2005)

fra³/fra⁴, mew^M6 has presumptive embryonic salivary gland phenotype

(Kolesnikov and Beckendorf, 2005)

mew^M6, sog^EP7 has wing vein | dorsal | somatic clone phenotype

(Araujo et al., 2003)

mew^M6, sog[+]/sog^EP7 has wing vein | dorsal | somatic clone phenotype

(Araujo et al., 2003)

mew^M6, scb² has embryonic/larval midgut | embryonic stage phenotype

(Martin-Bermudo et al., 1999)

if^B4, mew^M6 has embryonic/larval midgut | embryonic stage phenotype

(Martin-Bermudo et al., 1999)

if^B4, mew^M6 has somatic muscle cell phenotype

(Roote and Zusman, 1995)

if^k27e, mew^M6 has somatic muscle cell phenotype

(Roote and Zusman, 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Ret^C168 mew^M6 transheterozygous third instar larvae exhibit defects in class IV dendritic arborizing neuron dendrite crossing and a substantial loss of dendrite-ECM interaction. No phenotypes are observed in either heterozygote alone.

(Soba et al., 2015)

Reduced maintenance phenotype of adult mew^M6/mew^M6, if^k27e/if^k27e double mutant intestinal stem cell clones is partially rescued by expression of mew^UAS.cWa under the control of Scer\GAL4^Act.PU in these clones.

(Lin et al., 2013)

22.62% of mew^M6/mys¹ mutant class IV da dendrites (generated using the MARCM system under the control of Scer\GAL4^109(2)80) are enclosed in the epidermis rather than attached to the ECM. This compares to 4.09% and 4.18% in mys¹/+ and mew^M6/+ respectively.

mew^M6/wb⁰⁹⁴³⁷ mutant third instar larvae show a mild but statistically significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. The amount of enclosed dendrite seen in each heterozygote is similar to wild type.

mew^M6/LanA^9-32 mutant third instar larvae show a mild but statistically significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. The amount of enclosed dendrite seen in each heterozygote is similar to wild type.

mew^M6/LanB2^MB04039 mutant third instar larvae show a mild but statistically significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. The amount of enclosed dendrite seen in each heterozygote is similar to wild type.

(Han et al., 2012)

Embryos trans-heterozygous for βTub85Dⁿ and mew^M6 exhibit salivary gland migration defects.

(Jattani et al., 2009)

Hemizygous mew^M6 mutant embryos that are also heterozygous for Tsp^unspecified develop a weak muscle detachment phenotype affecting a small number of longitudinal muscles.

Hemizygous mew^M6/Y mutant embryos that are also homozygous for Tsp^Δ79 display only a mild enhancement of the Tsp^Δ79 single mutant muscle detachment phenotype.

(Chanana et al., 2007)

mew^M6/+ ; Df(3L)Exel6083/+ double heterozygotes show axon guidance defects characteristic of increased fasciculation in the ISNb pathway (73.6% of hemisegments), in the SNa pathway (55.3% of hemisegments) and in the central nervous system (40.5% of hemisegments). These defects are not seen in either single heterozygote.

(Huang et al., 2007)

mew^M6, if^k27e double mutant dorsal branch terminal cell clones show a loss of terminal branches and an increase in lumen density and complexity in the remaining branches.

(Levi et al., 2006)

mew^M6/+; sli²/+ embryos do not show defects in heart formation.

(MacMullin and Jacobs, 2006)

mew^M6 mys^b45 double heterozygotes show displacement of photoreceptor nuclei in the retina in 3% of cases.

(Curtin et al., 2005)

In mew^M6/Y; fra³/fra⁴ double mutant embryos, the misguided salivary gland phenotype is modified, compared to that of each single mutant. Like fra³/fra⁴ mutants, the majority (75%) of misguided glands curve laterally, but levels of penetrance are high, similar to mew^M6/Y single mutants.

(Kolesnikov and Beckendorf, 2005)

Small mys¹⁰ somatic clones in the wing generated a sog^EP7 background cause a distinctive vein phenotype: Dorsal clones located in the proximity of veins bend and displace the veins towards the clone, so that this vein runs along and outside the clone boundary. This vein shifting phenotype is observed for all longitudinal veins. However, clones that cross over a vein generate no phenotype, and neither do clones generated at a distance of three or more cells from a vein. Small ventrally located clones have no effect on vein formation.

(Araujo et al., 2003)

Salivary glands in mew^M6 scb² mutant embryos are indistinguishable from those in mew^M6 embryos.

(Bradley et al., 2003)

When tested in the odour-induced jump-test, double heterozygotes of mew^M6 and either mys^olfC-x3 or mys^olfC-x17 show reduced responses to both ethyl acetate and iso-amyl acetate.

(Ayyub and Paranjape, 2002)

In mew^M6/+ sli²/+ double heterozygous mutants the frequency of midline guidance errors is increased over the level observed in mew^M6 homozygous mutants. Less than 10% of segments revealed midline guidance errors in mew^M6/+ sli²/+ embryos. The frequency of midline crossing is much higher in mew^M6/Y;sli^unspecified/+ and also involves more lateral tracts.

(Stevens and Jacobs, 2002)

The midgut phenotype is enhanced in if^B4 mew^M6 double mutant embryos. if^{Scer\UAS.cMBa} does not rescue the migration defects of the midgut endodermal cells in mew^M6 embryos, when expressed under the control of Scer\GAL4^48Y. mew^M6 scb² double mutant embryos show a strong defect in the migration of the endodermal midgut cells, with a delay in migration of approximately 2 hours (similar to that observed in embryos lacking both maternal and zygotic mys function).

(Martin-Bermudo et al., 1999)

The frequency of wing blisters seen in mew^A1-5/mew^M6 flies is dominantly enhanced by pot^P3.

(Walsh and Brown, 1998)

if^{Scer\UAS.cMBa}, if^C.Scer\UAS or if^m8.Scer\UAS partially rescue the first instar lethality of mew^M6 homozygotes when expressed using Scer\GAL4^48Y, although the animals do not survive past the second larval instar stage. if::mew^{Scer\UAS.cMBa} partially rescues the first instar lethality of mew^M6 homozygotes when expressed using Scer\GAL4^48Y. The first instar lethality of mew^M6 homozygotes is rescued by if::mew^{Scer\UAS.cMBb} expressed using Scer\GAL4^48Y.

(Martin-Bermudo et al., 1997)

Scer\GAL4⁶⁸⁴ induced expression of if^C.Scer\UAS fails to rescue the blistering phenotype.

(Brabant et al., 1996)

Expression of if^C.Scer\UAS and if^m8.Scer\UAS also rescues the mutant phenotype in most of the clones with similar efficiency to mew^Scer\UAS.cWa.

(Roote and Zusman, 1996)

Double mutants for mew and if have a midgut phenotype as severe as that of mys mutants. Somatic muscle appears normal. Dorsal hole and U-shaped cuticle of mys mutants are not observed in mew or if mutants. Double mutants between mew⁴⁹⁸, mew⁰²³ or mew^M6 and if^k27e or if^B4 do not show the twisted germband, amnioserosal detachment, dorsal movement of germband, dorsal hole and U-shaped embryo phenotypes of mys mutants, but do show early muscle detachments, as seen for mys mutants.

(Roote and Zusman, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mew^M6 is rescued by Scer\GAL4^btl.PS/mew^UAS.cWa

(Boube et al., 2001)

mew^M6 is rescued by mew^UAS.cMBa/Scer\GAL4^48Y

(Martin-Bermudo et al., 1999)

mew^M6 is rescued by mew^UAS.cMBa/Scer\GAL4^48Y

(Martin-Bermudo et al., 1997)

mew^M6 is rescued by mew^UAS.cMBb/Scer\GAL4^48Y

(Martin-Bermudo et al., 1997)

mew^M6 is rescued by Scer\GAL4⁶⁸⁴/mew^UAS.cWa

(Brabant et al., 1996)

Comments

This lethality is rescued by mew^{Scer\UAS.cMBa} or mew^{Scer\UAS.cMBb} expressed using Scer\GAL4^48Y, with some larvae developing up to the third instar stage, although they do not develop into adults.

(Martin-Bermudo et al., 1997)

Scer\GAL4⁶⁸⁴ induced expression of mew^Scer\UAS.cWa rescues dorsal clones completely.

(Brabant et al., 1996)

Scer\GAL4^hs.PB mediated expression of mew^Scer\UAS.cWa in the clones completely rescues the disorganised photoreceptor phenotype in 85% of the clones and partially in the remaining clones.

(Roote and Zusman, 1996)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

1483

y¹ mew^M6 f^36a P{neoFRT}18A/FM7c

Kyoto

106340

y¹ mew^M6 f^36a P{neoFRT}18A/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mew⁶

(Jattani et al., 2009)

mew^M6

(Thuveson et al., 2019, Soba et al., 2015, Lin et al., 2013, Kim et al., 2012, Popodi et al., 2010-, Delon and Brown, 2009, Chen et al., 2008, Chanana et al., 2007, Huang et al., 2007, Levi et al., 2006, MacMullin and Jacobs, 2006, Curtin et al., 2005, Ayyub and Paranjape, 2002)

mew^m6

(Ismat et al., 2013, Boube et al., 2001, Martin-Bermudo, 2000, Martin-Bermudo et al., 1999, Roote and Zusman, 1996, Roote and Zusman, 1995)

Name Synonyms

Secondary FlyBase IDs

References (34)

Report Sections

Open Close