FlyBase Allele Report: Dmel\LanA[9-32]

General Information

Symbol

Dmel\LanA^9-32

Species

D. melanogaster

Name

FlyBase ID

FBal0029903

Feature type

allele

Associated gene

Dmel\LanA

Associated Insertion(s)

Carried in Construct

Also Known As

lamA^9.32, lamA^9-32

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Yarnitzky and Volk, 1995)

loss of function allele

(Rosenberg-Hasson et al., 1996, Henchcliffe et al., 1993)

Mutagen

Delta2-3 transposase

(Yarnitzky and Volk, 1995, Henchcliffe et al., 1993)

Progenitor genotype

P{hsneo}AS249

(Yarnitzky and Volk, 1995, Henchcliffe et al., 1993)

Cytology

Description

Deletion in genomic DNA upstream of the LanA gene, from 2.5kb upstream of the translational start site of LanA (site of insertion of progenitor, P{hsneo}AS249) to between nucleotides 370 and 806 of LanA, thereby deleting at least 370 bp of translated sequence of LanA.

(Henchcliffe et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult dorsal superficial tracheal branch

(Stark et al., 1997)

adult posterior midgut epithelium | somatic clone

(Lin et al., 2013)

amnioserosa

(Narasimha and Brown, 2004)

bouton & neuromuscular junction & embryo

(Prokop et al., 1998)

egg chamber | germline clone

(Frydman and Spradling, 2001)

embryo

(Schock and Perrimon, 2003)

embryonic/larval midgut & endoderm

(Yarnitzky and Volk, 1995)

extracellular matrix

(Wolfstetter and Holz, 2012)

fascicle

(Stevens and Jacobs, 2002)

larval pericardial cell | embryonic stage

(Yarnitzky and Volk, 1995)

larval somatic muscle cell

(Rosenberg-Hasson et al., 1996)

muscle attachment site & basal lamina

(Prokop et al., 1998)

muscle cell of abdominal 1 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 1 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 1 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 2 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 2 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 2 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 2 ventral oblique muscle 6 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 3 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 3 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 3 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 3 ventral oblique muscle 6 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 4 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 4 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 4 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 4 ventral oblique muscle 6 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 5 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 5 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 5 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 5 ventral oblique muscle 6 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 6 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 6 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 6 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 6 ventral oblique muscle 6 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 7 ventral acute muscle 3 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 7 ventral oblique muscle 4 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 7 ventral oblique muscle 5 | embryonic stage

(Yarnitzky and Volk, 1995)

muscle cell of abdominal 7 ventral oblique muscle 6 | embryonic stage

(Yarnitzky and Volk, 1995)

neuromuscular junction | third instar larval stage, with LanA^GD6022, Scer\GAL4^C57

(Tsai et al., 2012)

oocyte nucleus | cell non-autonomous | somatic clone

(Deng and Ruohola-Baker, 2000)

Detailed Description

Statement

Reference

Adult-generated LanA^9-32 homozygous mutant intestinal stem cell clones have reduced maintenance 7 days and 14 days after clone induction compared to control clones; remaining clones contain fewer cells by day 14.

(Lin et al., 2013)

Embryos mutant for LanA^9-32 do not exhibit any salivary gland invagination or migration defects.

(Patel and Myat, 2013)

The neuromuscular junction (NMJ) is normal in size in LanA^9-32/LanA^C01-190 mutant larvae.

Expression of LanA^GD6022 under the control of Scer\GAL4^C57 in a LanA^9-32/+ background results in an increase in NMJ size compared to wild type.

(Tsai et al., 2012)

Late hemizygous LanA^9-32 embryos show normal midgut morphology, but assembly of the extracellular matrix that normally surrounds it is severely disrupted.

(Wolfstetter and Holz, 2012)

The neuromuscular junction is normal in LanA^9-32/LanA²¹⁶ third instar larvae.

(Tsai et al., 2008)

Mutant embryos exhibit three defects in cellular organisation: detachment between the amnioserosa and the yolk cell; weak amnioserosa cell thinning; and apical expansion of the amnioserosa cells. However most embryos complete dorsal closure.

(Narasimha and Brown, 2004)

Maternal and zygotic mutant embryos have a range of defects. In the most severe cases, embryos disintegrate before the onset of retraction, in milder cases embryos exhibit fusion or deletion of segments and twisting, but no retraction defects.

(Schock and Perrimon, 2003)

Homozygous LanA^9-32 mutants exhibit a subtle phenotype, revealing midline guidance errors in approximately 4% of segments.

(Stevens and Jacobs, 2002)

germ-line clones for LanA^9-32 generate round eggs at a low frequency.

(Frydman and Spradling, 2001)

In a small proportion (4%) of LanA^9-32 mosaic egg chambers in which the posterior follicle cells are mutant, the oocyte nuclei remain posterior after stage 8, in contrast to wild-type.

(Deng and Ruohola-Baker, 2000)

Attachment of muscles to the epidermis is slightly disturbed in stage 16 homozygous embryos. Connecting hemiadherens junctions (HAJs) and tendon HAJs are normal in stage 17 embryos. The basement membrane is present, but is often fragmented and detaches from the unspecialised surfaces of the epidermis and muscles. The basement membrane remains attached to 88% of focal HAJs. The degree of adhesion of neuronal boutons to the muscle surface is reduced at the neuromuscular junctions.

(Prokop et al., 1998)

Embryos exhibit significant gaps in the dorsal trunk of the trachea.

(Stark et al., 1997)

Stage 15 and stage 16 Msp-300^sz75 LanA^9-32 double mutant embryos exhibit complete disruption of the muscle pattern.

(Rosenberg-Hasson et al., 1996)

The embryonic heart is 'broken', the pericardial cells are loosely associated with each other before dorsal closure and they dissociate from the cardioblasts after dorsal closure and migrate randomly. The somatic myotubules are narrow and twisted and may fall apart. The ventral oblique muscles never reach their proper attachment sites and exhibit an abnormal shape. The initial phase of columnar midgut endoderm polarisation is defective leading to improper arrangement of the endoderm along the visceral mesoderm.

(Yarnitzky and Volk, 1995)

Embryos, which are morphologically normal (with respect to CNS, PNS, motoneurons, muscles, head, mouthparts and denticle bands) and mobile nevertheless fail to hatch.

(Henchcliffe et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

LanA^9-32 has abnormal neuroanatomy phenotype, enhanceable by sli^unspecified

(Stevens and Jacobs, 2002)

Suppressed by

Statement

Reference

LanA^9-32 has abnormal neuroanatomy phenotype, suppressible by scb^unspecified

(Stevens and Jacobs, 2002)

Enhancer of

Statement

Reference

LanA^9-32/LanA[+] is an enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

LanA^9-32/LanA[+] is an enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

LanA^9-32/scb^unspecified is an enhancer of abnormal neuroanatomy phenotype of sli^unspecified

(Stevens and Jacobs, 2002)

LanA^9-32 is an enhancer of abnormal neuroanatomy phenotype of sli^unspecified

(Stevens and Jacobs, 2002)

Suppressor of

Statement

Reference

LanA^9-32/LanA[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^Mhc.PU, fz2^UAS.N

(Tsai et al., 2012)

LanA^9-32 is a suppressor of abnormal neuroanatomy phenotype of scb^unspecified

(Stevens and Jacobs, 2002)

Other

Statement

Reference

LanA^9-32, mys¹/mys[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

LanA^9-32, mew[+]/mew^M6 has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

Itgbn¹/betaInt-nu[+], LanA^9-32/LanA^C01-190 has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2012)

Fak^N30/Fak^KG00304, LanA^9-32/LanA[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2008)

Itgbn¹/betaInt-nu[+], LanA^9-32 has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Tsai et al., 2008)

LanA^9-32, scb^unspecified, sli² has abnormal neuroanatomy phenotype

(Stevens and Jacobs, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

LanA^9-32 has fascicle phenotype, enhanceable by sli^unspecified/scb^unspecified

(Stevens and Jacobs, 2002)

LanA^9-32 has fascicle phenotype, enhanceable by sli^unspecified

(Stevens and Jacobs, 2002)

Enhancer of

Statement

Reference

LanA^9-32/LanA[+] is an enhancer of heart primordium phenotype of sli²

(MacMullin and Jacobs, 2006)

LanA^9-32/LanA[+] is an enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

LanA^9-32/LanA[+] is an enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

sli²/LanA^9-32 is an enhancer of fascicle phenotype of scb^unspecified

(Stevens and Jacobs, 2002)

LanA^9-32/scb^unspecified is an enhancer of fascicle phenotype of sli^unspecified

(Stevens and Jacobs, 2002)

NOT Enhancer of

Statement

Reference

LanA^9-32/LanA[+] is a non-enhancer of wing phenotype of mys⁸

(Schock and Perrimon, 2003)

Suppressor of

Statement

Reference

LanA^9-32/LanA[+] is a suppressor of neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^Mhc.PU, fz2^UAS.N

(Tsai et al., 2012)

LanA^9-32 is a suppressor of central nervous system phenotype of scb^unspecified

(Stevens and Jacobs, 2002)

Other

Statement

Reference

LanA^9-32, mys¹/mys[+] has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Han et al., 2012)

LanA^9-32, mew[+]/mew^M6 has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Han et al., 2012)

Itgbn¹/betaInt-nu[+], LanA^9-32/LanA^C01-190 has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2012)

LanA^9-32, scb²/scb[+] has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

Itgbn¹/betaInt-nu[+], LanA^9-32 has NMJ bouton | increased number | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, LanA^9-32/LanA[+] has NMJ bouton | increased number | third instar larval stage phenotype

(Tsai et al., 2008)

Itgbn¹/betaInt-nu[+], LanA^9-32 has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2008)

LanA^9-32, scb^unspecified has fascicle phenotype

(Stevens and Jacobs, 2002)

LanA^9-32, scb^unspecified, sli^unspecified has central nervous system phenotype

(Stevens and Jacobs, 2002)

LanA^9-32, if^B4 has muscle attachment site & basal lamina phenotype

(Prokop et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

mys¹/LanA^9-32 mutant third instar larvae show a mild but statistically significant increase in the proportion of dorsal midline ddaC dendritic length that is enclosed within the epidermis rather than attached to the ECM The amount of enclosed dendrite seen in each heterozygote is similar to wild type.

mew^M6/LanA^9-32 mutant third instar larvae show a mild but statistically significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. The amount of enclosed dendrite seen in each heterozygote is similar to wild type.

(Han et al., 2012)

βInt-ν¹/+ ; LanA^9-32/LanA^C01-190 third instar larvae show overgrowth of the neuromuscular junction.

The reduction in neuromuscular junction size seen in third instar larvae expressing fz2^Scer\UAS.N under the control of Scer\GAL4^Mhc.PU is suppressed if they are also heterozygous for LanA^9-32.

(Tsai et al., 2012)

LanA^9-32/+ ; scb²/+ double heterozygous embryos show defects in alignment of the cardioblasts at the dorsal midline.

(Vanderploeg et al., 2012)

Significant overgrowth of the NMJ (increased bouton number per muscle area) is seen in LanA^9-32/+ Fak^N30/Fak^KG00304 third instar larvae.

Significant overgrowth of the NMJ (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in βInt-ν¹/+ LanA^9-32/+ third instar larvae.

(Tsai et al., 2008)

LanA^9-32/+; sli²/+ embryos show heart defects that include contralaterally placed cardial cell nuclei and laterally placed cardial cells.

(MacMullin and Jacobs, 2006)

The addition of LanA^9-32/+ to mys⁸ hemizygotes does not enhance the wing blister phenotype seen in these animals.

(Schock and Perrimon, 2003)

LanA^9-32 mutants, when double heterozygous with sli^unspecified, exhibit midline crossover of Fas2-labelled axons in over 30% of segments. Midline guidance errors are not visible in LanA^9-32/+; scb^unspecified/+ double mutants, in contrast to single mutant heterozygotes. However, defasciculation and interruptions to the Fas2-labelling of the most lateral fascicle are observed. The degree of defasciculation and midline guidance errors in all axon tracts of the triple heterozygote of scb^unspecified/sli^unspecified;LanA^9-32/+, appears to be additive of the individual phenotypes. In addition, a narrowing of the central nervous system and the medial displacement of all axon tracts is also seen in this mutant combination.

(Stevens and Jacobs, 2002)

if^B4 LanA^9-32 double mutant embryos show each of the defects seen in if^B4 or LanA^9-32 single mutant embryos, and in addition show loss of basement membrane adhesion to the epidermal HAJs.

(Prokop et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99936

w^*; LanA^9-32/TM6B, P{Dfd-EYFP}3, Sb¹ Tb⁺ ca¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

LamA^9.32

(Rosenberg-Hasson et al., 1996, Yarnitzky and Volk, 1995)

LanA^9-32

(Wolfstetter et al., 2019, Patel and Myat, 2013, Han et al., 2012, Tsai et al., 2012, Vanderploeg et al., 2012, Wolfstetter and Holz, 2012, Tsai et al., 2008, Inoue and Hayashi, 2007)

lamA^9-32

(Araujo et al., 2003, Stark et al., 1997)

lamA^9.32

(Prokop et al., 1998)

lamininA^9.32

(Narasimha and Brown, 2004, Narasimha and Brown, 2004)

lanA^9-32

(Matsubayashi et al., 2017, Lin et al., 2013, Knox et al., 2011, MacMullin and Jacobs, 2006, Schneider et al., 2006, Stevens and Jacobs, 2002, Deng and Ruohola-Baker, 2000)

lanA^9.32

(Koper et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (26)

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