FlyBase Allele Report: Dmel\fz2[UAS.N]

General Information

Symbol

Dmel\fz2^UAS.N

Species

D. melanogaster

Name

FlyBase ID

FBal0092982

Feature type

allele

Associated gene

Dmel\fz2

Associated Insertion(s)

Carried in Construct

P{UAS-fz2.N}

Also Known As

UAS-DFz2N, UAS-DFz2-DN

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhang and Carthew, 1998)

Progenitor genotype

fz2^UAS.cZa

(Zhang and Carthew, 1998)

Carried in construct

P{UAS-fz2.N}

Cytology

Description

Coding sequence downstream of nucleotide 174 (FBrf0089594) replaced with coding sequence nucleotides 174-1030, plus sequence RWL-stop. The result is a fusion of extracellular and first transmembrane domains with an intracellular DTRWL at the carboxy-terminus, analogous to fz^UAS.N.

(Zhang and Carthew, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

fz2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ato.3.6

(Vandewalle et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mhc.PU

(Tsai et al., 2012)

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Zhang and Carthew, 1998)

visible, with Scer\GAL4^69B

(Lee et al., 2009, Zhang and Carthew, 1998)

visible, with Scer\GAL4^bbg-C96

(Lin and Perrimon, 1999)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell | adult stage, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

eye, with Scer\GAL4^hs.2sev

(Zhang and Carthew, 1998)

eye disc morphogenetic furrow | ectopic, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

lobula columnar neuron LC14 | adult stage, with Scer\GAL4^ato.3.6

(Vandewalle et al., 2013)

medulla | adult stage, with Scer\GAL4^ato.3.6

(Vandewalle et al., 2013)

mitochondrial crista | adult stage, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

mitochondrion | adult stage, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

neuromuscular junction | third instar larval stage, with Scer\GAL4^Mhc.PU

(Tsai et al., 2012)

ommatidium, with Scer\GAL4^hs.2sev

(Zhang and Carthew, 1998)

presumptive embryonic salivary gland, with Scer\GAL4^fkh.PZ

(Harris and Beckendorf, 2007)

wing, with Scer\GAL4^69B

(Zhang and Carthew, 1998)

wing, with Scer\GAL4^bbg-C96

(Lin and Perrimon, 1999)

wing, with Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

wing, with Scer\GAL4^sd-SG29.1

(Zeng and Verheyen, 2004)

wing margin, with Scer\GAL4^69B

(Lee et al., 2009)

wing margin, with Scer\GAL4^dpp.blk1

(Lee et al., 2009)

wing margin, with Scer\GAL4^sd-SG29.1

(Zeng and Verheyen, 2004)

wing margin bristle, with Scer\GAL4^bbg-C96

(Lin and Perrimon, 1999)

Detailed Description

Statement

Reference

The expression of fz2^Scer\UAS.N under the control of Scer\GAL4^Mhc.PW leads to the dorsal longitudinal indirect flight muscles exhibiting mitochondria defects, including abnormal morphology, sparse and collapsed cristae, and a significant decrease in electron density in electron microscopy images at 21 days post eclosion, as compared to controls.

(Li et al., 2016)

Scer\GAL4^Bx-MS1096-mediated expression causes the loss or strong reduction of wings.

(Chang and Sun, 2014)

When fz2^Scer\UAS.N is expressed in dorsal cluster neurons under the control of Scer\GAL4^ato.3.6 the branching pattern in the adult medulla is disturbed, giving rise to an increased number of branches at the 3rd branching point of the grid-like structure.

(Vandewalle et al., 2013)

Third instar larvae expressing fz2^Scer\UAS.N under the control of Scer\GAL4^Mhc.PU show a reduction in neuromuscular junction size compared to controls.

(Tsai et al., 2012)

One copy of fz2^Scer\UAS.N has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

Expression of fz2^Scer\UAS.N under the control of Scer\GAL4^69B results in severely notched wings.

Expression of fz2^Scer\UAS.N in the anteroposterior wing boundary using Scer\GAL4^dpp.blk1 results in a mild wing notching phenotype.

(Lee et al., 2009)

Expression of fz2^Scer\UAS.N under the control of Scer\GAL4^fkh.PZ results in salivary gland guidance defects; a large portion of the salivary gland curves towards the central nervous system, with the curving beginning early, as the gland migrates along the circular visceral mesoderm.

(Harris and Beckendorf, 2007)

fz2^Scer\UAS.N; Scer\GAL4^sd-SG29.1 flies have highly reduced wings lacking all margin structures and much of the outer area of the wing blade.

(Zeng and Verheyen, 2004)

When expressed from Scer\GAL4^ptc-559.1 the apical cells of the ovarian sheath remain clustered in the apical region of the ovary, indicating that they are not capable of basal migration.

(Cohen et al., 2002)

Expression of fz2^Scer\UAS.N under the control of Scer\GAL4^dpp.blk1 results in ectopic furrow formation in the eye disc.

(Kumar and Moses, 2001)

Ectopic expression of fz2^Scer\UAS.N at the presumptive wing margin using Scer\GAL4^C96 is associated with a fully penetrant mild wing margin defect.

(Lin and Perrimon, 1999)

When expression is driven by Scer\GAL4^hs.2sev, the two chiral forms of ommatidia are intermixed in the dorsal and ventral half of the eye. When expression is driven by Scer\GAL4^69B, significant wing margin and bristle loss occurs, with reduction in wing size. When expression is driven by Scer\GAL4^69B, ectopic wing bristle phenotype of fz2^Scer\UAS.cZa is suppressed.

(Zhang and Carthew, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^bbg-C96, fz2^UAS.N has visible phenotype, enhanceable by dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

Scer\GAL4^69B, fz2^UAS.N has visible phenotype, enhanceable by wg^l-17

(Zhang and Carthew, 1998)

Suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, fz2^UAS.N has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Itgbn¹/betaInt-nu[+]

(Tsai et al., 2012)

Scer\GAL4^Mhc.PU, fz2^UAS.N has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by LanA^9-32/LanA[+]

(Tsai et al., 2012)

Scer\GAL4^69B, fz2^UAS.N has visible phenotype, suppressible by Hipk^UAS.Tag:HA

(Lee et al., 2009)

Enhancer of

Statement

Reference

fz2[+]/fz2^UAS.N is an enhancer of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, fz2^UAS.N has wing margin phenotype, enhanceable by Hipk⁴

(Lee et al., 2009)

Scer\GAL4^bbg-C96, fz2^UAS.N has wing phenotype, enhanceable by dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

Scer\GAL4^bbg-C96, fz2^UAS.N has wing margin bristle phenotype, enhanceable by dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, fz2^UAS.N has wing phenotype, non-enhanceable by Cow^UAS.cCa, Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

Scer\GAL4^Bx-MS1096, fz2^UAS.N has wing phenotype, non-enhanceable by Cow^RNAi.UAS, Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

Scer\GAL4^Bx-MS1096, fz2^UAS.N has wing phenotype, non-enhanceable by Cow^RNAi.UAS.1, Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

Suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, fz2^UAS.N has neuromuscular junction | third instar larval stage phenotype, suppressible by LanA^9-32/LanA[+]

(Tsai et al., 2012)

Scer\GAL4^Mhc.PU, fz2^UAS.N has neuromuscular junction | third instar larval stage phenotype, suppressible by Itgbn¹/betaInt-nu[+]

(Tsai et al., 2012)

Scer\GAL4^69B, fz2^UAS.N has wing margin phenotype, suppressible by Hipk^UAS.Tag:HA

(Lee et al., 2009)

Scer\GAL4^sd-SG29.1, fz2^UAS.N has wing margin phenotype, suppressible | partially by nmo^DB24/nmo^DB24

(Zeng and Verheyen, 2004)

Scer\GAL4^sd-SG29.1, fz2^UAS.N has wing phenotype, suppressible | partially by nmo^DB24/nmo^DB24

(Zeng and Verheyen, 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, fz2^UAS.N has wing phenotype, non-suppressible by Cow^UAS.cCa, Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

Scer\GAL4^Bx-MS1096, fz2^UAS.N has wing phenotype, non-suppressible by Cow^RNAi.UAS, Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

Scer\GAL4^Bx-MS1096, fz2^UAS.N has wing phenotype, non-suppressible by Cow^RNAi.UAS.1, Scer\GAL4^Bx-MS1096

(Chang and Sun, 2014)

Enhancer of

Statement

Reference

fz2[+]/fz2^UAS.N is an enhancer of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in neuromuscular junction size seen in third instar larvae expressing fz2^Scer\UAS.N under the control of Scer\GAL4^Mhc.PU is suppressed if they are also heterozygous for either LanA^9-32 or βInt-ν¹.

(Tsai et al., 2012)

Expression of hipk^{Scer\UAS.T:Ivir\HA1} in animals expressing fz2^Scer\UAS.N under the control of Scer\GAL4^69B rescues the severely notched wing phenotype.

Removing one copy of hipk⁴ in animals expressing fz2^Scer\UAS.N in the anteroposterior wing boundary using Scer\GAL4^dpp.blk1 enhances the wing notching phenotype, resulting in moderate to severely truncated wings.

(Lee et al., 2009)

Wing size reduction and loss of wing margin structures in fz2^Scer\UAS.N; Scer\GAL4^sd-SG29.1 flies is significantly suppressed by nmo^DB24/nmo^DB24.

(Zeng and Verheyen, 2004)

The wing margin defect produced by expression of fz2^Scer\UAS.N using Scer\GAL4^C96 is strongly enhanced by dally⁰⁶⁴⁶⁴/dally⁰⁶⁴⁶⁴.

(Lin and Perrimon, 1999)

The phenotype caused by expression of fz2^Scer\UAS.N using fz2^Scer\UAS.N is slightly dominantly enhanced by heterozygosity for wg^l-17.

(Zhang and Carthew, 1998)

Xenogenetic Interactions

Statement

Reference

One copy of fz2^Scer\UAS.N moderately enhances the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Rescued by

fz2^UAS.N is rescued by fz2^UAS.cZa/Scer\GAL4^69B

(Zhang and Carthew, 1998)

Rescues

fz2^UAS.N/Scer\GAL4^69B rescues fz2^UAS.cZa

(Zhang and Carthew, 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fz2^Scer\UAS.N

fz2^UAS.N

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

Open Close