Dfz2, Dfrz2, fz-2, Dfrizzled2, DFz-2
Gene model reviewed during 5.55
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884.
Gene model reviewed during 5.45
Gene model reviewed during 6.02
5.5 (northern blot)
Interacts with ATP6AP2.
Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fz2 using the Feature Mapper tool.
fz2 transcript is first detected in a stripe at about 15-70% egg length in stage 6 embryos. By stage 8, a segmental expression pattern is visible, and at stage 10, 15 stripes are discernible in the presumptive head, trunk, posterior midgut primordium, in a subset of the cells at the anterior midgut invagination site, and in the procephalic lobe. At stage 12, fz2 expression is strong in the midgut and visceral mesoderm. Starting at stage 9, fz2 is also expressed in the central nervous system (CNS). At the end of embryogenesis, fz2 transcript is present in the CNS, the dorsal vessel, and the hindgut. Northern analysis shows that fz2 transcript is expressed in all developmental stages, with the highest levels during embryogenesis and in late larval and pupal life.
fz2 protein is uniformly expressed in immature hemocyte precursors of the lymph gland during the first larval instar through mid-second instar. At mid-to-late second instar, fz2 protein expression is down-regulated in maturing hemocytes expressing the hemocyte marker Hml.
fz2 protein was observed in glutamatergic type 1b synaptic boutons at the larval neuromuscular junction. The protein was observed both pre- and post-synaptically although it was enriched in post-synaptic areas and co-localized with dlg1 protein staining.
fz2 protein expression is not detectable by immunolocalization at stage 6 of embryogenesis, when fz2 transcript is first detected. The transcript and protein expression patterns coincide on and after stage 9/10 of embryogenesis. fz2 protein is expressed in the embryonic central nervous system starting at stage 9, and continuing through the rest of embryogenesis. At stage 10, the posterior midgut primordium, and at stages 12-16, the visceral mesoderm, also expresses fz2 protein.
GBrowse - Visual display of RNA-Seq signalsView Dmel\fz2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Identified as a candidate gene for hypoxia-specific selection (via an experimental evolution paradigm) that is also differentially expressed between control and hypoxia-adapted larvae.
fz2 is required for normal salivary gland migration in the embryo. It is required in the second phase of salivary gland migration, as the gland moves posteriorly within the embryo.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
High levels of fz2 protein stabilise wg protein, allowing it to reach cells far from its site of synthesis. The expression of fz2 is repressed by wg signaling, creating a gradient of decreasing wg protein stability moving toward the dorso-ventral boundary. The repression of fz2 is essential for the normal shape of the wg morphogen gradient as well as the response of cells to the wg signal. In contrast to other ligand-receptor relationships where the receptor limits diffusion of the ligand, fz2 broadens the range of wg protein action by protecting it from degradation.