FlyBase Gene Report: Dmel\Wnt2

General Information

Symbol

Dmel\Wnt2

Species

D. melanogaster

Name

Wnt oncogene analog 2

Annotation Symbol

CG1916

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004360

Gene Model Status

Current

Stock Availability

39 publicly available

Gene Summary

Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Segment polarity protein. May function in gonadogenesis and limb development. Wg and Wnt2 have a role in the developing trachea and together are responsible for all dorsal trunk formation. (UniProt, P28465)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

DWnt-2, DWnt2, Wnt-2

Key Links

Genomic Location

Cytogenetic map

45E1-45E1

Sequence location

2R:9,494,166..9,503,188 [+]

Recombination map

2-60

RefSeq locus

NT_033778 REGION:9494166..9503188

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables frizzled binding

inferred from physical interaction with FLYBASE:fz; FB:FBgn0001085,FLYBASE:fz2; FB:FBgn0016797,FLYBASE:fz3; FB:FBgn0027343

(Wu and Nusse, 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables cytokine activity

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(GO Reference Genome Project, 2011-)

enables frizzled binding

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(GO Reference Genome Project, 2011-)

enables signaling receptor binding

inferred from sequence or structural similarity with FLYBASE:wg; FB:FBgn0284084

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR005817

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in axon extension

inferred from mutant phenotype

(Srahna et al., 2006)

involved_in canonical Wnt signaling pathway

inferred from mutant phenotype

(Herr and Basler, 2012)

involved_in germ-line stem-cell niche homeostasis

inferred from mutant phenotype

(Wang et al., 2015)

involved_in muscle organ development

inferred from mutant phenotype

(Kozopas and Nusse, 2002)

involved_in open tracheal system development

inferred from genetic interaction with FLYBASE:wg; FB:FBgn0284084

(Llimargas and Lawrence, 2001)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in canonical Wnt signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(GO Reference Genome Project, 2011-)

involved_in cell fate commitment

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(GO Reference Genome Project, 2011-)

involved_in neuron differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(GO Reference Genome Project, 2011-)

involved_in positive regulation of JNK cascade

inferred from biological aspect of ancestor with PANTHER:PTN000246687

(GO Reference Genome Project, 2011-)

involved_in Wnt signaling pathway

inferred from electronic annotation with InterPro:IPR005817

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from electronic annotation with InterPro:IPR005817

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:wg; FB:FBgn0284084

(Gene Ontology Curators, 2002-)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

WNTs

Pathway (FlyBase)

WNT-TCF SIGNALING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Belongs to the Wnt family. (P28465)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

WNTs -: WNTs are evolutionarily conserved secreted Cys-rich glycoproteins, defined by sequence homology to the original members of the family - Wnt1 in mouse and wingless (wg) in Drosophila. They are extracellular ligands for members of the Frizzled family of receptors as well as other receptors. (Adapted from PMID:23151663).

Pathway (FlyBase)

WNT-TCF SIGNALING PATHWAY CORE COMPONENTS -: The canonical Wnt signaling pathway is initiated by the binding of a Wnt ligand to a frizzled family receptor on the cell surface. Activation of the pathway leads to the inhibition of cytoplasmic β-catenin (arm) degradation and its subsequent accumulation in the nucleus, where it regulates the transcription of target genes. (Adapted from FBrf0218499 and FBrf0223299).

Protein Function (UniProtKB)

(UniProt, P28465)

Summary (Interactive Fly)

ligand - Wnt homolog - required for the development of the sheath of the male reproductive tract and testis morphogenesis - mediates redox regulation - maintains the germ line stem cell differentiation niche

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Wnt2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P28465)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Wnt2-RA

FBtr0088512

NM_057462

2240

352

Wnt2-RB

FBtr0339383

NM_001299322

2235

325

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Wnt2-PA

FBpp0087596

39.8

352

9.36

FBpp0308474

36.4

325

9.19

A0A0B4LFY4

NP_001286251

AHN56049

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

352 (aa); 39.9 (kD predicted)

(Russell et al., 1992)

Comments

External Data

Post Translational Modification

Palmitoleoylated by porcupine. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport Wnt2 to unique sites at the cell surface. Depalmitoleoylated by notum, leading to inhibit Wnt signaling pathway.

(UniProt, P28465)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Wnt2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Wnt2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.78

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

organism | 10-70% egg length

Comment: 15-70% egg length

(Russell et al., 1992)

embryonic stage 13

organism | posterior

(Russell et al., 1992)

embryonic stage 13 -- 17

organism | anterior

(Russell et al., 1992)

embryonic stage 15

gonad

(Kozopas et al., 1998)

embryonic stage 16

gonad | posterior

(Kozopas et al., 1998)

wandering third instar larval stage

male genital disc primordium

(Kozopas et al., 1998)

pupal stage P6

seminal vesicle | restricted

Comment: reference states 24 hr APF

(Kozopas et al., 1998)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Russell et al., 1992)

embryonic stage 7

proctodeum

(Russell et al., 1992)

embryonic stage 10

maxillary segment

(Russell et al., 1992)

labial segment

(Russell et al., 1992)

embryonic stage 10 -- 11

abdominal segment 1 -- abdominal segment 8 | restricted

(Russell et al., 1992)

thoracic segment of embryo

(Russell et al., 1992)

embryonic stage 11

labrum

(Russell et al., 1992)

embryonic stage 14 -- 17

gonad | posterior

(Russell et al., 1992)

Additional Descriptive Data

Wnt2 transcript is first detected in stage 13 embryos, in the mesodermal cells which will form the somatic mesoderm of the gonad. Wnt2 is expressed in the gonad at stage 15, and this expression is limited to the posterior portion of the gonad at stage 16. In late third instar larvae, Wnt2 expression is detected in male genital primordia. In the 24 hr male pupal genital disc, expression is detected in the epithelial cells at the apical tip of the developing seminal vesicle. Wnt2 transcript is not detected in female structures; however, when Wnt2 is ectopically expressed in third instar larval females, male-specific pigment cells are formed in the resulting adults.

(Russell et al., 1992)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Wnt2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Wnt2

View exonic expression by tissue for Dmel\Wnt2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35975/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004360

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004360

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004360

Flygut - An atlas of the Drosophila adult midgut

FBgn0004360

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 28 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Wnt2

Transgenic constructs containing regulatory region of Wnt2

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(2R)brp^6.1

(Fouquet et al., 2009)

Df(2R)BSC408

(Christensen et al., 2008.4.15)

Gene not disrupted in

Df(2R)Np5

(Georgiev et al., 2012)

Df(2R)w45-19g

(Georgiev et al., 2012)

Df(2R)w73-1

(Georgiev et al., 2012)

Df(2R)wun-GL

(Georgiev et al., 2012)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^ptc-559.1

Wnt2^UAS.cSa

lethal | embryonic stage, with Scer\GAL4^btl.PS

Wnt2^UAS.cSa

lethal | larval stage, with Scer\GAL4^btl.PS

Wnt2^UAS.cSa

lethal - all die before end of pupal stage, with Scer\GAL4^αTub84B.PC

Wnt2^UAS.cSa

partially lethal

Wnt2^unspecified

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Wnt2^GD5736

viable, with Scer\GAL4^Mef2.PR

Wnt2^GD5736

viable, with Scer\GAL4^pnr-MD237

Wnt2^GD5736

viable, with Scer\GAL4^tin.CΔ4

Wnt2^GD5736

Sterility

Allele

male semi-sterile (with Wnt2^L)

Wnt2^O

male semi-sterile (with Wnt2^O)

Wnt2^L

male sterile

Wnt2^unspecified

male sterile | recessive

Other Phenotypes

Allele

abnormal neuroanatomy | dominant

Wnt2^O

flightless | heat sensitive

Wnt2^RJ

some die during pupal stage, with Scer\GAL4^αTub84B.PC

Wnt2^UAS.cSa

visible

Wnt2^I

visible | heat sensitive (with Df(2R)11)

Wnt2^RJ

visible | recessive

Phenotype manifest in

Allele

axon & dorsal cluster neuron

Wnt2^O

direct flight muscle cell

Wnt2^unspecified

direct flight muscle cell, with Scer\GAL4^upd1-E132

Wnt2^UAS.cKa

direct flight muscle cell | heat sensitive (with Df(2R)11)

Wnt2^RJ

female germline stem cell | decreased number, with Scer\GAL4^C587

Wnt2^UAS.cKa

larval tracheal system, with Scer\GAL4^ptc-559.1

Wnt2^UAS.cSa

larval tracheal system, with Scer\GAL4^VP16.arm

Wnt2^UAS.cSa

muscle cell of wing axillary muscle I1, with Scer\GAL4^upd1-E132

Wnt2^UAS.cKa

muscle cell of wing axillary muscle III1

Wnt2^O

muscle cell of wing axillary muscle III1 | heat sensitive

Wnt2^RJ

muscle cell of wing basalar muscle b1, with Scer\GAL4^upd1-E132

Wnt2^UAS.cKa

muscle cell of wing basalar muscle b3, with Scer\GAL4^upd1-E132

Wnt2^UAS.cKa

ovary | somatic clone, with Scer\GAL4^Act5C.PP

Wnt2^UAS.cKa

tergopleural muscle cell

Wnt2^O

tergopleural muscle cell | heat sensitive (with Df(2R)11)

Wnt2^RJ

testis

testis (with Wnt2^L)

Wnt2^O

testis (with Wnt2^O)

Wnt2^L

testis pigment cell

testis pigment cell | ectopic, with Scer\GAL4^Act5C.PP

Wnt2^UAS.cKa

testis pigment cell | heat sensitive

Wnt2^I

testis pigment cell | heat sensitive (with Df(2R)11)

Wnt2^RJ

testis sheath

testis sheath muscle cell

testis sheath muscle cell | heat sensitive

Wnt2^I

tracheal dorsal trunk primordium, with Scer\GAL4^btl.PS

Wnt2^UAS.cSa

wing

wing | heat sensitive (with Df(2R)11)

Wnt2^RJ

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\WNT7B

13 of 14

Yes

Hsap\WNT7A

12 of 14

Yes

Hsap\WNT4

7 of 14

Yes

Hsap\WNT2B

6 of 14

Yes

Hsap\WNT2

5 of 14

Yes

Hsap\WNT5B

5 of 14

Hsap\WNT3A

4 of 14

Yes

Hsap\WNT5A

4 of 14

Hsap\WNT16

4 of 14

Yes

Hsap\WNT3

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\WNT1

2 of 14

Hsap\WNT6

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (19)

Rnor\Wnt7b

13 of 14

Yes

Rnor\Wnt7a

12 of 14

Yes

Rnor\Wnt4

7 of 14

Yes

Rnor\Wnt2b

6 of 14

Yes

Rnor\Wnt2

4 of 14

Yes

Rnor\Wnt3a

4 of 14

Yes

Rnor\Wnt5a

4 of 14

Rnor\Wnt5b

4 of 14

Rnor\Wnt16

4 of 14

Yes

Rnor\Wnt3

3 of 14

Yes

Rnor\Wnt8a

3 of 14

Rnor\Wnt8b

3 of 14

Yes

Rnor\Wnt9a

3 of 14

Rnor\Wnt9b

3 of 14

Rnor\Wnt11

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Mus musculus (laboratory mouse) (19)

Mmus\Wnt7b

13 of 14

Yes

Mmus\Wnt7a

12 of 14

Yes

Mmus\Wnt4

7 of 14

Yes

Mmus\Wnt2b

5 of 14

Yes

Mmus\Wnt2

5 of 14

Yes

Mmus\Wnt3a

4 of 14

Yes

Mmus\Wnt5a

4 of 14

Mmus\Wnt16

4 of 14

Yes

Mmus\Wnt3

3 of 14

Yes

Mmus\Wnt5b

3 of 14

Mmus\Wnt8a

3 of 14

Yes

Mmus\Wnt8b

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Wnt1

2 of 14

Mmus\Wnt6

2 of 14

Mmus\Wnt9a

2 of 14

Xenopus tropicalis (Western clawed frog) (29)

Xtro\wnt7b

7 of 13

Yes

Xtro\wnt7a

6 of 13

Yes

Xtro\wnt7c

6 of 13

Yes

Xtro\wnt2b

3 of 13

Xtro\wnt2

3 of 13

Yes

Xtro\wnt5a

3 of 13

Xtro\LOC100487954

2 of 13

Yes

Xtro\wnt3

2 of 13

Yes

Xtro\wnt4

2 of 13

Xtro\wnt5b

2 of 13

Yes

Xtro\wnt16

2 of 13

Yes

Xtro\adck2

1 of 13

Yes

Xtro\ccdc78

1 of 13

Yes

Xtro\chmp4c

1 of 13

Xtro\gstk1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (25)

Drer\wnt7bb

13 of 14

Yes

Drer\LOC568110

12 of 14

Yes

Drer\wnt7aa

12 of 14

Yes

Drer\wnt7ba

12 of 14

Yes

Drer\wnt2ba

6 of 14

Yes

Drer\wnt2bb

6 of 14

Yes

Drer\wnt4

6 of 14

Yes

Drer\wnt2

5 of 14

Yes

Drer\wnt3a

4 of 14

Yes

Drer\wnt4b

4 of 14

Yes

Drer\wnt16

4 of 14

Yes

Drer\wnt3

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Drer\wnt6a

2 of 14

Drer\wnt6b

2 of 14

Drer\wnt11

2 of 14

Yes

Drer\wnt1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\cwn-2

6 of 14

Yes

Cele\egl-20

5 of 14

Yes

Cele\cwn-1

3 of 14

Cele\mom-2

3 of 14

Cele\lin-44

2 of 14

Yes

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP010283

12 of 12

Yes

Agam\AgaP_AGAP010290

4 of 12

Agam\AgaP_AGAP008678

3 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

Yes

Agam\AgaP_AGAP009735

2 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Wnt2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

Wnt5

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

WNT7A; Wnt family member 7A

model of Schinzel type phocomelia

IEA

(FlyBase, 2019-)

model of Fuhrmann syndrome

IEA

(FlyBase, 2019-)

WNT4; Wnt family member 4

model of Mullerian aplasia and hyperandrogenism

IEA

(FlyBase, 2019-)

WNT3; Wnt family member 3

model of tetraamelia syndrome 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Wnt2^I

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Dupont et al., 2012)

Wnt2^O

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Dupont et al., 2012)

Wnt2^RJ

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Dupont et al., 2012)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

WNT7B; Wnt family member 7B

13 of 14

601967

WNT7A; Wnt family member 7A

12 of 14

601570

WNT4; Wnt family member 4

7 of 14

603490

Mullerian aplasia and hyperandrogenism

WNT2B; Wnt family member 2B

6 of 14

601968

DIARRHEA 9; DIAR9

WNT2; Wnt family member 2

5 of 14

147870

WNT5B; Wnt family member 5B

5 of 14

606361

WNT3A; Wnt family member 3A

4 of 14

606359

WNT5A; Wnt family member 5A

4 of 14

164975

ROBINOW SYNDROME, AUTOSOMAL DOMINANT 1; DRS1

autosomal dominant Robinow syndrome 1

WNT16; Wnt family member 16

4 of 14

606267

WNT3; Wnt family member 3

3 of 14

165330

TETRAAMELIA SYNDROME 1; TETAMS1

tetraamelia syndrome 1

WNT8A; Wnt family member 8A

3 of 14

606360

WNT8B; Wnt family member 8B

3 of 14

601396

WNT9A; Wnt family member 9A

3 of 14

602863

WNT9B; Wnt family member 9B

3 of 14

602864

WNT10A; Wnt family member 10A

3 of 14

606268

WNT10B; Wnt family member 10B

3 of 14

601906

split hand-foot malformation 6

WNT11; Wnt family member 11

3 of 14

603699

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Wnt2 - dlp

anti tag coimmunoprecipitation, anti tag western blot

(Tu et al., 2020, Waghmare et al., 2020)

Wnt2 - fz

affinity technology, enzyme linked immunosorbent assay, inferred by author

(Wu and Nusse, 2002)

Wnt2 - fz2

affinity technology, enzyme linked immunosorbent assay, inferred by author

(Wu and Nusse, 2002)

Wnt2 - fz3

affinity technology, enzyme linked immunosorbent assay, inferred by author

(Wu and Nusse, 2002)

Wnt2 - otk

anti tag coimmunoprecipitation, anti tag western blot, inferred by author, affinity technology, fluorescence microscopy

(Linnemannstöns et al., 2014)

Wnt2 - otk2

inferred by author, affinity technology, fluorescence microscopy, anti tag coimmunoprecipitation, anti tag western blot

(Linnemannstöns et al., 2014)

Wnt2 - por

anti tag coimmunoprecipitation, anti tag western blot

(Herr and Basler, 2012)

Wnt2 - wls

anti bait coimmunoprecipitation, anti tag western blot

(Herr and Basler, 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Wnt2|wg

suppressible

arm

(Llimargas and Lawrence, 2001)

Wnt2

suppressible

Axn

(Willert et al., 1999)

Wnt2

suppressible

pan

(Willert et al., 1999)

Wnt2|wg

suppressible

Wnt6

(Llimargas and Lawrence, 2001)

Wnt2|wg

suppressible

wntD

(Llimargas and Lawrence, 2001)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dlp

suppressible

Wnt2

(Waghmare et al., 2020)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61806

DroID - A comprehensive database of gene and protein interactions.

FBgn0004360

MIST (genetic) - An integrated Molecular Interaction Database

35975

MIST (protein-protein) - An integrated Molecular Interaction Database

35975

Pathways

Signaling Pathways (FlyBase)

WNT-TCF SIGNALING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

WNT ligand biogenesis and trafficking

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P28465

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-60

Cytogenetic map

45E1-45E1

Sequence location

2R:9,494,166..9,503,188 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45E1-45E1

Limits computationally determined from genome sequence between P{lacW}wun^k09507&P{lacW}l(2)k12402^k12402 and P{EP}EP2165&P{lacW}l(2)k01301^k01301

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45E-45E

(Kozopas, 1996.4.29)

45E-45E

(determined by in situ hybridisation)

(Russell et al., 1992)

Experimentally Determined Recombination Data

Location

2-60

(FlyBase, 2016)

2-59.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (39)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

6961

P{UAS-Wnt2.K}5A, w¹¹⁸

6908

wg^l-17 Wnt2^L/CyO

48039

w¹¹¹⁸; P{GMR22F02-GAL4}attP2

48974

w¹¹¹⁸; P{GMR22C06-GAL4}attP2

48994

w¹¹¹⁸; P{GMR22F10-GAL4}attP2

6958

Wnt2^O/CyO, amos^Roi-1

6960

Wnt2^I/CyO, amos^Roi-1

68626

w¹¹¹⁸; P{R22C06-p65.AD}attP40

FlyORF

F000939

M{UAS-Wnt2.ORF.3xHA}ZH-86Fb

Korea

15515

P{GMR21H03-GAL4}attP2

VDRC

v104338

P{KK107678}VIE-260B

v38077

w¹¹¹⁸; P{GD5736}v38077

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE36604

Other clones

FI20276

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004360

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004360

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Wnt2 promotes correct patterning of direct flight muscles through a mechanism that is independent of the attachment site differentiation initiated by sr.

(Kozopas and Nusse, 2002)

Wnt2 assists wg to specify the main tracheal trunk. Wnt2 affects tracheal development but apperently has no effect on the cuticle, whereas wg influences both.

(Llimargas and Lawrence, 2001)

Candidate gene for testis length quantitative trait locus.

(Macdonald and Goldstein, 1999)

Wnt2 is required for cell fate determination and morphogenesis in the developing male reproductive tract.

(Kozopas et al., 1998)

Wnt2 encodes an amino terminal signal peptide suggesting the gene product is a secreted protein.

(Russell et al., 1992)

Sequence analysis of Wnt genes is performed in several species to determine the ancestral lineage of the gene family.

(Sidow, 1992)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG1916

(Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Seinen et al., 2010, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Perkins et al., 2009)

D-wnt-2

(Boyle and DiNardo, 1995)

DWnt-2

(Chen et al., 2008, Kozopas and Nusse, 2002, Kozopas, 2000, Willert et al., 1999, Kozopas et al., 1998, Kozopas et al., 1998, Perrimon and Nusse, 1998, Cadigan and Nusse, 1997, Kozopas et al., 1997, Nusse, 1997, Nusse, 1997, Kozopas, 1996.4.29, Graba et al., 1995, Siegfried and Perrimon, 1994, Russell et al., 1992)

DWnt2

(Yu et al., 2020, Rothenbusch-Fender et al., 2017, Port et al., 2008, Coudreuse and Korswagen, 2007, Bajpai et al., 2004, Wu and Nusse, 2002, Llimargas and Lawrence, 2001, Rizkalla and Siegfried, 2000, Reifegerste and Moses, 1999)

Dm DWnt2

(Konikoff et al., 2010)

Dm-2

(Sidow, 1992)

Dwnt-2

(Swarup and Verheyen, 2012, Moore et al., 1998, Boyle et al., 1997, Kozopas et al., 1996, Williamson and Lehmann, 1996)

Dwnt2

(Perochon et al., 2018, Dupont et al., 2012)

Wnt

(Blair, 1999)

Wnt-2

(DeFalco et al., 2003, Jockusch and Ober, 2000, Wehrli and Tomlinson, 1998, Nusse, 1992.3.2)

Wnt2

(Wang et al., 2025, Collins et al., 2024, Cui et al., 2024, Goins et al., 2024, Rajeev et al., 2024, Deshpande et al., 2022, Destalminil-Letourneau et al., 2021, Everetts et al., 2021, Cui et al., 2020, Rushton et al., 2020, Tu et al., 2020, Waghmare et al., 2020, Xia et al., 2020, Camara et al., 2019, Story et al., 2019, Aughey et al., 2018, Grobler et al., 2018, Ripp et al., 2018, Tian et al., 2018, Waghmare and Page-McCaw, 2018, Transgenic RNAi Project members, 2017-, Wang et al., 2015, Hamada-Kawaguchi et al., 2014, Linnemannstöns et al., 2014, Schertel et al., 2013, Southall et al., 2013, Webber et al., 2013, Georgiev et al., 2012, Herr and Basler, 2012, Lahaye et al., 2012, Whitworth et al., 2012, Chatterjee et al., 2011, McNeil et al., 2011, Chau et al., 2009, Christensen et al., 2008.4.15, DeFalco et al., 2008, Hendrix et al., 2008, Harris and Beckendorf, 2007, Junion et al., 2007, Zeitlinger et al., 2007, Biemar et al., 2006, Casal et al., 2006, Pickeral et al., 2000)

dWnt2

(Baranski et al., 2001)

wnt-2

(Deshpande et al., 2016)

wnt2

(Chen et al., 2022, Nguyen et al., 2022, Ewen-Campen et al., 2020, Luo et al., 2015, Zhu and Zhang, 2013, Chen et al., 2008, Srahna et al., 2006, Glise et al., 2005)

Name Synonyms

Wnt oncogene analog 2

(Chatterjee et al., 2011, Chau et al., 2009)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35975

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P28465

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P28465

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LFY4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P28465

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35975/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004360

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004360

FlyMine - An integrated database for Drosophila genomics

FBgn0004360

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1916

MARRVEL_MODEL - MARRVEL (model organism gene)

35975

Linkouts

BioGRID - A database of protein and genetic interactions.

61806

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004360

DroID - A comprehensive database of gene and protein interactions.

FBgn0004360

DRSC - Results frm RNAi screens

FBgn0004360

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Wnt2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004360

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004360

Flygut - An atlas of the Drosophila adult midgut

FBgn0004360

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004360

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

35975

MIST (protein-protein) - An integrated Molecular Interaction Database

35975

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

WNT ligand biogenesis and trafficking

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P28465

References (153)

The following external sites may use different assemblies or annotations than FlyBase.

2.78

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