FB2025_01 , released February 20, 2025
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Takada, R., Hijikata, H., Kondoh, H., Takada, S. (2005). Analysis of combinatorial effects of Wnts and Frizzleds on beta-catenin/armadillo stabilization and Dishevelled phosphorylation.  Genes Cells 10(9): 919--928.
FlyBase ID
FBrf0187604
Publication Type
Research paper
Abstract
Both Wnt ligands and Frizzled (Fz) receptors each constitute a large family in vertebrates, but the receptor specificity of each Wnt has remained largely unknown. Here, we examined the receptor specificity of two typical Wnts, Wnt-3a and Wnt-5a, in signal transmission. To investigate systematically the combinatorial effects of these Wnts, various Fzs on canonical Wnt/beta-catenin signaling, we analyzed the ability of these Wnt proteins to increase stability of armadillo/beta-catenin proteins in Drosophila S2 cells expressing vertebrate Fzs. Wnt-3a increases the amount of armadillo proteins in cells expressing Fzs 4, 5 and 8, but not Fzs 3 and 6; whereas Wnt-5a does not increase it in any cell line. In contrast, both Wnt-3a and Wnt-5a increase the phosphorylation of Dsh in combination with most of the Fzs. This Dsh phosphorylation is abrogated by decreasing the levels of casein kinase I alpha by double-stranded RNA-mediated translational interference. These observations indicate that both Wnt proteins can interact with the majority of Fz receptors and elicit signaling reactions exemplified by Dsh phosphorylation but that the stabilization of beta-catenin/armadillo proteins in the Wnt/beta-catenin signaling occurs only when specific combinations of Wnt and Fz meet.
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Cells
    Title
    Genes to cells : devoted to molecular & cellular mechanisms
    Publication Year
    1996-
    ISBN/ISSN
    1356-9597
    Data From Reference
    Genes (5)