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General Information
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| Term |
fluorescent resonance energy transfer |
ID (Ontology) |
MI:0055 (Molecular Interactions) |
| Definition |
FRET is a quantum mechanical process involving the radiationless transfer of energy from a donor fluorophore to an appropriately positioned acceptor fluorophore. The fluorophores are genetically fused to the protein in analysis and cotransfected. Three basic conditions must be fulfilled for FRET to occur between a donor molecule and acceptor molecule. First, the donor emission spectrum must significantly overlap the absorption spectrum of the acceptor. Second, the distance between the donor and acceptor fluorophores must fall within the range 20 to 100 Angstrom. Third, the donor and acceptor fluorophores must be in favourable orientations.[ PubMed:11558993 ] |
| Also Known As |
"FRET" ; "fret" ; "FRET analysis" (for all, see Synonyms field below) |
| Comment |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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fluorescent resonance energy transfer (all annotations which use CV term, excluding "NOT" statements) | 115 |
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Relationships