FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

fluorescent resonance energy transfer

ID (Ontology)

MI:0055 (Molecular Interactions)

Definition

FRET is a quantum mechanical process involving the radiationless transfer of energy from a donor fluorophore to an appropriately positioned acceptor fluorophore. The fluorophores are genetically fused to the protein in analysis and cotransfected. Three basic conditions must be fulfilled for FRET to occur between a donor molecule and acceptor molecule. First, the donor emission spectrum must significantly overlap the absorption spectrum of the acceptor. Second, the distance between the donor and acceptor fluorophores must fall within the range 20 to 100 Angstrom. Third, the donor and acceptor fluorophores must be in favourable orientations.[ PubMed:11558993 ]

Also Known As

"FRET" ; "fret" ; "FRET analysis" (for all, see Synonyms field below)

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
fluorescent resonance energy transfer (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  biophysical
   |__fluorescence technology
       |__fluorescent resonance energy transfer  115 rec.

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Relationships
Is a	fluorescence technology
Part of
Synonyms & Secondary IDs
Synonyms
	"FRET" EXACT PSI-MI-alternate "fret" EXACT PSI-MI-short "FRET analysis" EXACT PSI-MI-alternate "RET" EXACT PSI-MI-alternate
Secondary IDs

External Crossreferences & Linkouts