FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Wnk
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General Information
Symbol
Dmel\Wnk
Species
D. melanogaster
Name
Wnk kinase
Annotation Symbol
CG7177
Feature Type
protein_coding_gene
FlyBase ID
FBgn0037098
Gene Model Status
Current
Stock Availability
15 publicly available
Enzyme Name (EC)
Gene Summary
Wnk kinase (Wnk) encodes a chloride-sensitive Ser/Thr kinase that signals through a downstream target Ser/Thr kinase, encoded by fray, which is activated by phosphorylation the product of Wnk. Its roles include regulation of ion transport and cell growth, and differentiation and developmental patterning, including roles in canonical Wnt signaling and induction of the product of Awh. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DmWNK

Key Links
Genomic Location
Cytogenetic map
78D5-78D6
Sequence location
Recombination map
3-47
RefSeq locus
NT_037436 REGION:21543850..21554928
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables molecular condensate scaffold activity
inferred from direct assay
(Boyd-Shiwarski et al., 2022)
enables protein kinase activity
inferred from direct assay
(Serysheva et al., 2013)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR024678
(InterPro Project Members, 2004-)
enables potassium channel inhibitor activity
inferred from biological aspect of ancestor with PANTHER:PTN000357109
(GO Reference Genome Project, 2011-)
enables protein kinase activity
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271
(InterPro Project Members, 2004-)
enables protein serine/threonine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000357044
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR024678
(InterPro Project Members, 2004-)
Biological Process (9 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in cell volume homeostasis
inferred from direct assay
(Boyd-Shiwarski et al., 2022)
involved_in cellular hyperosmotic response
inferred from direct assay
(Boyd-Shiwarski et al., 2022)
involved_in membraneless organelle assembly
inferred from direct assay
(Boyd-Shiwarski et al., 2022)
involved_in positive regulation of canonical Wnt signaling pathway
inferred from mutant phenotype
(Serysheva et al., 2013)
inferred from genetic interaction with FLYBASE:dsh; FB:FBgn0000499
(Serysheva et al., 2013)
inferred from genetic interaction with FLYBASE:fz2; FB:FBgn0016797
(Serysheva et al., 2013)
involved_in positive regulation of potassium ion import across plasma membrane
inferred from mutant phenotype
(Wu et al., 2014)
involved_in wing disc development
inferred from mutant phenotype
(Serysheva et al., 2013)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in intracellular signal transduction
inferred from biological aspect of ancestor with PANTHER:PTN000357044
(GO Reference Genome Project, 2011-)
involved_in monoatomic ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000357109
(GO Reference Genome Project, 2011-)
involved_in negative regulation of sodium ion transport
inferred from biological aspect of ancestor with PANTHER:PTN000357109
(GO Reference Genome Project, 2011-)
involved_in positive regulation of potassium ion import across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000357109
(GO Reference Genome Project, 2011-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000357044
(GO Reference Genome Project, 2011-)
located_in cytoplasm
inferred from sequence or structural similarity with UniProtKB:Q9H4A3
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. (M9PGC5)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
protein serine/threonine kinase activity
Summaries
Gene Snapshot
Wnk kinase (Wnk) encodes a chloride-sensitive Ser/Thr kinase that signals through a downstream target Ser/Thr kinase, encoded by fray, which is activated by phosphorylation the product of Wnk. Its roles include regulation of ion transport and cell growth, and differentiation and developmental patterning, including roles in canonical Wnt signaling and induction of the product of Awh. [Date last reviewed: 2019-03-21]
Gene Group (FlyBase)
UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -
This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.
Pathway (FlyBase)
POSITIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY -
Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
Protein Function (UniProtKB)
Serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (PubMed:23797875, PubMed:25086033, PubMed:36318922). Wnk mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed:36318922). The membraneless compartment concentrates Wnk with its substrate Fray, promoting Wnk-dependent phosphorylation and activation of downstream kinase Fray. Following activation, Fray catalyzes phosphorylation of ion cotransporters Ncc69 and Irk1, regulating their activity (PubMed:25086033, PubMed:35303418, PubMed:36318922). Phosphorylation of Na-K-Cl cotransporter Ncc69 promotes its activation and ion influx (PubMed:25086033). Involved in circadian rhythms in small ventral lateral (sLNv) pacemaker neurons: in the morning, Wnk activity is repressed by high levels of intracellular chloride; in contrast Wnk activation in the evening promotes the activation of the inwardly rectifying potassium channel Irk1 via Fray (PubMed:35303418). Acts as a positive regulator of the canonical Wnt signaling pathway during wing disk development (PubMed:23797875).
(UniProt, M9PGC5)
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Wnk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry M9PGC5)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 6.02

GC splice donor site postulated

Gene model reviewed during 6.08

Gene model reviewed during 5.46

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Unconventional translation start (AUU) postulated; supported by species conservation; FlyBase analysis.

Gene model reviewed during 6.03

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Wnk-RL
FBtr0346817
NM_001275245
7916
2441
Wnk-RM
FBtr0346818
NM_001275242
8089
2462
Wnk-RN
FBtr0346819
NM_001275243
8199
2441
Wnk-RO
FBtr0347540
NM_001275244
7916
2243
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Wnk-PL
FBpp0312392
270.3
2441
6.61
M9PD81
NP_001262174
AGB94867
Wnk-PM
FBpp0312393
272.8
2462
6.81
M9PGC5
NP_001262171
AGB94864
Wnk-PN
FBpp0312394
270.3
2441
6.61
M9PD81
NP_001262172
AGB94865
Wnk-PO
FBpp0312580
248.9
2243
6.45
M9PIH3
NP_001262173
AGB94866
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2441 aa isoforms: Wnk-PL, Wnk-PN
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Post Translational Modification

Autophosphorylated (PubMed:23797875). Autophosphorylation at Ser-628 and Ser-632 promotes its activity (By similarity).

(UniProt, M9PGC5)
Domain

Disordered regions undergo liquid-liquid phase separation (LLPS) for the formation of a cytoplasmic membraneless compartment that concentrates Wnk with its downstream substrates.

(UniProt, M9PGC5)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Wnk using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.35

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Wnk in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 26 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Wnk
Transgenic constructs containing regulatory region of Wnk
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Scer\GAL4Mef2.PR
lethal (with Df(3L)ED4978)
lethal (with WnkAA16.DsRed+)
lethal (with Wnkex22)
lethal (with WnkMB06499)
lethal | recessive
partially lethal, with Scer\GAL4tin.CΔ4
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4pnr-MD237
viable (with Df(3L)ED4978)
viable (with WnkAA16)
viable (with WnkAA21)
viable (with Wnkex22)
viable (with WnkMB06499)
Sterility
Allele
female semi-sterile, with Scer\GAL4VP16.mat.αTub67C
Other Phenotypes
Allele
abnormal locomotor rhythm | adult stage, with Scer\GAL4Clk.-856
abnormal locomotor rhythm | adult stage, with Scer\GAL4P2.4.Pdf
abnormal locomotor rhythm | adult stage, with Scer\GAL4tim.PE
abnormal neuroanatomy | adult stage, with Scer\GAL4ato.3.6
abnormal neuroanatomy | adult stage, with Scer\GAL4elav-C155
abnormal neuroanatomy | adult stage, with Scer\GAL4Gr21a.PC
abnormal neuroanatomy | adult stage, with Scer\GAL4Or88a.PF
abnormal neuroanatomy | adult stage, with Scer\GAL4pnr-MD237
abnormal neuroanatomy | adult stage, with Scer\GAL4pnr-MD237, WnkHMC05157
abnormal neuroanatomy | adult stage, with Scer\GAL4pnr-MD237, WnkKK102654
abnormal neuroanatomy | adult stage | progressive | somatic clone
abnormal neuroanatomy | embryonic stage, with Scer\GAL4insc-Mz1407
abnormal neuroanatomy | P-stage | somatic clone
abnormal neuroanatomy | P-stage | somatic clone, with Scer\GAL4pnr-MD237, WnkHMC05157
abnormal neuroanatomy | P-stage | somatic clone, with Scer\GAL4pnr-MD237, WnkKK102654
abnormal neuroanatomy | somatic clone | third instar larval stage
abnormal size, with Scer\GAL4en.PU
abnormal size, with Scer\GAL4nub.PU
decreased size | adult stage, with Scer\GAL4Bx-MS1096
decreased size | adult stage, with Scer\GAL4en.PU
decreased size | larval stage, with Scer\GAL4en.PU
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
visible, with Scer\GAL4hh.PU
visible, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4sd.PU
visible | somatic clone
Phenotype manifest in
Allele
abdominal segment | pharate adult stage, with Scer\GAL4sd.PU
abdominal segment | pharate adult stage | somatic clone
adult Malpighian tubule, with Scer\GAL4c42
adult mechanosensory neuron, with Scer\GAL4pnr-MD237
adult mechanosensory neuron | somatic clone
adult thoracic mechanosensory chaeta neuron | P-stage | somatic clone
adult thoracic mechanosensory chaeta neuron | P-stage | somatic clone, with Scer\GAL4pnr-MD237, WnkHMC05157
adult thoracic mechanosensory chaeta neuron | P-stage | somatic clone, with Scer\GAL4pnr-MD237, WnkKK102654
axon | adult stage, with Scer\GAL4ato.3.6
axon | adult stage, with Scer\GAL4elav-C155
axon | adult stage, with Scer\GAL4Gr21a.PC
axon | adult stage, with Scer\GAL4Or88a.PF
axon | adult stage, with Scer\GAL4pnr-MD237
axon | adult stage, with Scer\GAL4pnr-MD237, WnkHMC05157
axon | adult stage, with Scer\GAL4pnr-MD237, WnkKK102654
axon | adult stage | somatic clone
axon | P-stage | somatic clone
axon | P-stage | somatic clone, with Scer\GAL4pnr-MD237, WnkHMC05157
axon | P-stage | somatic clone, with Scer\GAL4pnr-MD237, WnkKK102654
larval peripheral nervous system, with Scer\GAL4insc-Mz1407
lobula columnar neuron LC14 | adult stage, with Scer\GAL4ato.3.6
macrochaeta | somatic clone
mechanosensory neuron of posterior dorsocentral bristle | adult stage, with Scer\GAL4pnr-MD237, WnkHMC05157
mechanosensory neuron of posterior dorsocentral bristle | adult stage, with Scer\GAL4pnr-MD237, WnkKK102654
mechanosensory neuron of posterior dorsocentral bristle | adult stage | somatic clone
mechanosensory neuron of posterior scutellar bristle | adult stage, with Scer\GAL4pnr-MD237
mechanosensory neuron of posterior scutellar bristle | adult stage, with Scer\GAL4pnr-MD237, WnkHMC05157
mechanosensory neuron of posterior scutellar bristle | adult stage, with Scer\GAL4pnr-MD237, WnkKK102654
mechanosensory neuron of posterior scutellar bristle | adult stage | somatic clone
microchaeta | somatic clone
posterior wing margin, with Scer\GAL4en.PU
posterior wing margin | somatic clone
rhabdomere of eye photoreceptor cell
trichogen cell, with Scer\GAL4pnr-MD237
wing | posterior, with Scer\GAL4en.PU
wing | posterior, with Scer\GAL4nub.PU
wing blade, with Scer\GAL4Bx-MS1096
wing blade posterior compartment, with Scer\GAL4en.PU
wing disc posterior compartment, with Scer\GAL4en.PU
wing hair, with Scer\GAL4en.PU
wing margin, with Scer\GAL4sd.PU
wing margin bristle, with Scer\GAL4en.PU
wing margin bristle | somatic clone
wing vein | ectopic, with Scer\GAL4hh.PU
wing vein | ectopic | somatic clone
wing vein L4, with Scer\GAL4hh.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (53)
Hsap\WNK2
7 of 14
Yes
Yes
 
1  
Hsap\WNK3
7 of 14
Yes
Yes
2  
Hsap\WNK1
6 of 14
No
Yes
2  
Hsap\WNK4
6 of 14
No
Yes
Hsap\NRBP1
3 of 14
No
No
1  
Hsap\NRBP2
3 of 14
No
No
Hsap\ABL2
1 of 14
No
No
2  
Hsap\BRSK1
1 of 14
No
No
Hsap\BUB1B-PAK6
1 of 14
No
No
Hsap\DSTYK
1 of 14
No
Yes
Hsap\EPHA2
1 of 14
No
No
Hsap\EPHA6
1 of 14
No
No
Hsap\EPHB2
1 of 14
No
No
1  
Hsap\FGFR1
1 of 14
No
No
Hsap\JAK2
1 of 14
No
No
Hsap\LRRK2
1 of 14
No
No
50  
Hsap\MAP3K1
1 of 14
No
Yes
Hsap\MAP3K2
1 of 14
No
No
Hsap\MAP3K3
1 of 14
No
No
Hsap\MAP3K5
1 of 14
No
No
Hsap\MAP3K9
1 of 14
No
No
Hsap\MAP3K10
1 of 14
No
No
1  
Hsap\MAP3K11
1 of 14
No
No
Hsap\MAP3K12
1 of 14
No
No
Hsap\MAP3K13
1 of 14
No
No
Hsap\MAP3K15
1 of 14
No
No
Hsap\MAP3K19
1 of 14
No
No
Hsap\MAP3K20
1 of 14
No
No
Hsap\MAP3K21
1 of 14
No
No
Hsap\NEK4
1 of 14
No
No
Hsap\NEK6
1 of 14
No
No
Hsap\NEK7
1 of 14
No
No
Hsap\NEK9
1 of 14
No
No
1  
Hsap\NEK11
1 of 14
No
No
Hsap\NIM1K
1 of 14
No
No
Hsap\PAK1
1 of 14
No
No
Hsap\PAK2
1 of 14
No
No
Hsap\PAK3
1 of 14
No
No
Hsap\PAK4
1 of 14
No
No
Hsap\PAK6
1 of 14
No
No
Hsap\PLK3
1 of 14
No
No
Hsap\SCYL1
1 of 14
No
No
Hsap\SCYL2
1 of 14
No
No
Hsap\SCYL3
1 of 14
No
No
Hsap\SNRK
1 of 14
No
No
1  
Hsap\STK3
1 of 14
No
No
1  
Hsap\STK4
1 of 14
No
No
2  
Hsap\STK10
1 of 14
No
No
Hsap\STK24
1 of 14
No
No
Hsap\STK39
1 of 14
No
No
Hsap\TAOK1
1 of 14
No
No
Hsap\TAOK3
1 of 14
No
No
Hsap\TNK2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (35)
Rnor\Wnk2
7 of 14
Yes
Yes
Rnor\Wnk3
7 of 14
Yes
Yes
Rnor\Wnk4
7 of 14
Yes
Yes
Rnor\Wnk1
6 of 14
No
Yes
Rnor\Nrbp1
3 of 14
No
No
Rnor\Nrbp2
3 of 14
No
No
Rnor\Dstyk
1 of 14
No
Yes
Rnor\Epha6
1 of 14
No
No
Rnor\Fgfr1
1 of 14
No
No
Rnor\Jak2
1 of 14
No
No
Rnor\Lrrk1
1 of 14
No
No
Rnor\Map3k2
1 of 14
No
No
Rnor\Map3k3
1 of 14
No
No
Rnor\Map3k9
1 of 14
No
No
Rnor\Map3k10
1 of 14
No
No
Rnor\Map3k11
1 of 14
No
No
Rnor\Map3k12
1 of 14
No
No
Rnor\Map3k13
1 of 14
No
No
Rnor\Map3k19
1 of 14
No
No
Rnor\Map3k20
1 of 14
No
No
Rnor\Map3k21
1 of 14
No
No
Rnor\Mark2
1 of 14
No
No
Rnor\Nek4
1 of 14
No
No
Rnor\Nek7
1 of 14
No
No
Rnor\Pak1
1 of 14
No
No
Rnor\Pak2
1 of 14
No
No
Rnor\Pak3
1 of 14
No
No
Rnor\Pak6
1 of 14
No
No
Rnor\Scyl1
1 of 14
No
No
Rnor\Scyl2
1 of 14
No
No
Rnor\Scyl3
1 of 14
No
No
Rnor\Slk
1 of 14
No
No
Rnor\Snrk
1 of 14
No
No
Rnor\Stk10
1 of 14
No
No
Rnor\Tnk2
1 of 14
No
No
Mus musculus (laboratory mouse) (36)
Mmus\Wnk2
7 of 14
Yes
Yes
Mmus\Wnk3
7 of 14
Yes
Yes
Mmus\Wnk1
6 of 14
No
Yes
Mmus\Wnk4
6 of 14
No
Yes
Mmus\Nrbp2
3 of 14
No
No
Mmus\Nrbp1
2 of 14
No
No
Mmus\Dstyk
1 of 14
No
Yes
Mmus\Epha6
1 of 14
No
No
Mmus\Fgfr1
1 of 14
No
No
Mmus\Jak2
1 of 14
No
No
Mmus\Map3k2
1 of 14
No
No
Mmus\Map3k3
1 of 14
No
No
Mmus\Map3k9
1 of 14
No
No
Mmus\Map3k10
1 of 14
No
No
Mmus\Map3k11
1 of 14
No
No
Mmus\Map3k12
1 of 14
No
No
Mmus\Map3k13
1 of 14
No
No
Mmus\Map3k19
1 of 14
No
Yes
Mmus\Map3k20
1 of 14
No
No
Mmus\Map3k21
1 of 14
No
No
Mmus\Mark2
1 of 14
No
No
Mmus\Nek4
1 of 14
No
No
Mmus\Nek5
1 of 14
No
No
Mmus\Nek7
1 of 14
No
No
Mmus\Nek11
1 of 14
No
No
Mmus\Pak1
1 of 14
No
No
Mmus\Pak2
1 of 14
No
No
Mmus\Pak3
1 of 14
No
No
Mmus\Pak6
1 of 14
No
No
Mmus\Scyl1
1 of 14
No
No
Mmus\Scyl2
1 of 14
No
No
Mmus\Scyl3
1 of 14
No
No
Mmus\Slk
1 of 14
No
No
Mmus\Snrk
1 of 14
No
No
Mmus\Srms
1 of 14
No
No
Mmus\Stk10
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (33)
Xtro\wnk3
5 of 13
Yes
Yes
Xtro\atp6ap1.2
4 of 13
No
No
Xtro\mbtps2
3 of 13
No
No
Xtro\wnk1
2 of 13
No
Yes
Xtro\abl2
1 of 13
No
No
Xtro\epha6
1 of 13
No
No
Xtro\igf1r
1 of 13
No
No
Xtro\irgq
1 of 13
No
Yes
Xtro\LOC100486125
1 of 13
No
No
Xtro\map3k2
1 of 13
No
No
Xtro\map3k10
1 of 13
No
No
Xtro\map3k13
1 of 13
No
No
Xtro\map3k19
1 of 13
No
No
Xtro\map3k20
1 of 13
No
No
Xtro\MGC76064
1 of 13
No
Yes
Xtro\nek9
1 of 13
No
No
Xtro\nek11
1 of 13
No
No
Xtro\nrbp1
1 of 13
No
No
Xtro\nrbp2
1 of 13
No
Yes
Xtro\pak2
1 of 13
No
No
Xtro\pak3
1 of 13
No
No
Xtro\pak4
1 of 13
No
No
Xtro\scyl1
1 of 13
No
No
Xtro\scyl2
1 of 13
No
No
Xtro\scyl3
1 of 13
No
No
Xtro\sik3
1 of 13
No
Yes
Xtro\stk3
1 of 13
No
No
Xtro\stk4
1 of 13
No
No
Xtro\stk10
1 of 13
No
No
Xtro\stk39
1 of 13
No
No
Xtro\tmem267
1 of 13
No
No
Xtro\tnk2
1 of 13
No
No
Xtro\wnk4
1 of 13
No
Yes
Danio rerio (Zebrafish) (42)
Drer\wnk1a
7 of 14
Yes
Yes
Drer\wnk2
7 of 14
Yes
Yes
Drer\si:dkey-151g10.3
6 of 14
No
Yes
Drer\wnk1b
6 of 14
No
Yes
Drer\wnk4b
6 of 14
No
Yes
Drer\nrbp1
3 of 14
No
No
Drer\nrbp2a
3 of 14
No
No
Drer\nrbp2b
3 of 14
No
No
Drer\dstyk
1 of 14
No
Yes
Drer\ek1
1 of 14
No
No
Drer\ephb3a
1 of 14
No
No
Drer\hunk
1 of 14
No
Yes
Drer\insrb
1 of 14
No
No
Drer\irak1
1 of 14
No
No
Drer\jak3
1 of 14
No
No
Drer\LOC100536197
1 of 14
No
No
Drer\LOC101883538
1 of 14
No
Yes
Drer\LOC101886393
1 of 14
No
No
Drer\map3k2
1 of 14
No
No
Drer\map3k3
1 of 14
No
No
Drer\map3k10
1 of 14
No
No
Drer\map3k12
1 of 14
No
No
Drer\map3k15
1 of 14
No
No
Drer\map3k19
1 of 14
No
No
Drer\map3k21
1 of 14
No
No
Drer\map3k22
1 of 14
No
No
Drer\nek2
1 of 14
No
No
Drer\nek4
1 of 14
No
No
Drer\nek7
1 of 14
No
No
Drer\pak2a
1 of 14
No
No
Drer\pak2b
1 of 14
No
No
Drer\pak4
1 of 14
No
No
Drer\pak6b
1 of 14
No
No
Drer\sb:eu779
1 of 14
No
No
Drer\scyl1
1 of 14
No
No
Drer\scyl2
1 of 14
No
No
Drer\scyl3
1 of 14
No
No
Drer\si:ch211-45c16.2
1 of 14
No
No
Drer\si:zfos-2326c3.2
1 of 14
No
No
Drer\taok2a
1 of 14
No
No
Drer\tnk2b
1 of 14
No
No
Drer\zak
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (13)
Cele\wnk-1
7 of 14
Yes
Yes
Cele\hpo-11
2 of 14
No
No
Cele\dlk-1
1 of 14
No
No
Cele\max-2
1 of 14
No
No
Cele\mlk-1
1 of 14
No
No
Cele\nekl-3
1 of 14
No
No
Cele\nsy-1
1 of 14
No
No
Cele\par-1
1 of 14
No
No
Cele\pkc-2
1 of 14
No
No
Cele\scyl-1
1 of 14
No
No
Cele\Y52D5A.2
1 of 14
No
No
Cele\zak-1
1 of 14
No
No
Cele\ZC581.9
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (22)
Agam\AgaP_AGAP012422
4 of 12
Yes
Yes
Agam\AgaP_AGAP007996
2 of 12
No
No
Agam\AgaP_AGAP000489
1 of 12
No
No
Agam\AgaP_AGAP000747
1 of 12
No
No
Agam\AgaP_AGAP001091
1 of 12
No
No
Agam\AgaP_AGAP001752
1 of 12
No
No
Agam\AgaP_AGAP001790
1 of 12
No
No
Agam\AgaP_AGAP002142
1 of 12
No
No
Agam\AgaP_AGAP002710
1 of 12
No
No
Agam\AgaP_AGAP002986
1 of 12
No
No
Agam\AgaP_AGAP003202
1 of 12
No
No
Agam\AgaP_AGAP005207
1 of 12
No
No
Agam\AgaP_AGAP006044
1 of 12
No
No
Agam\AgaP_AGAP006461
1 of 12
No
No
Agam\AgaP_AGAP006573
1 of 12
No
No
Agam\AgaP_AGAP008317
1 of 12
No
No
Agam\AgaP_AGAP009032
1 of 12
No
No
Agam\AgaP_AGAP009730
1 of 12
No
No
Agam\AgaP_AGAP009961
1 of 12
No
No
Agam\AgaP_AGAP010874
1 of 12
No
No
Agam\AgaP_AGAP012421
1 of 12
No
Yes
Agam\AgaP_AGAP012423
1 of 12
No
Yes
Arabidopsis thaliana (thale-cress) (77)
Atha\WNK1
7 of 13
Yes
Yes
Atha\WNK2
7 of 13
Yes
Yes
Atha\WNK3
7 of 13
Yes
Yes
Atha\WNK4
7 of 13
Yes
Yes
Atha\WNK5
7 of 13
Yes
Yes
Atha\WNK6
7 of 13
Yes
Yes
Atha\WNK7
7 of 13
Yes
Yes
Atha\WNK9
7 of 13
Yes
Yes
Atha\AT5G55560
6 of 13
No
Yes
Atha\WNK8
6 of 13
No
Yes
Atha\WNK10
6 of 13
No
Yes
Atha\AT1G04700
1 of 13
No
No
Atha\AT1G16270
1 of 13
No
No
Atha\AT1G18160
1 of 13
No
No
Atha\AT1G22870
1 of 13
No
No
Atha\AT1G23700
1 of 13
No
No
Atha\AT1G51790
1 of 13
No
Yes
Atha\AT1G61860
1 of 13
No
No
Atha\AT1G67890
1 of 13
No
No
Atha\AT1G71410
1 of 13
No
No
Atha\AT1G73660
1 of 13
No
No
Atha\AT1G74360
1 of 13
No
Yes
Atha\AT1G79570
1 of 13
No
No
Atha\AT1G79640
1 of 13
No
No
Atha\AT2G35050
1 of 13
No
No
Atha\AT3G06620
1 of 13
No
No
Atha\AT3G06630
1 of 13
No
No
Atha\AT3G06640
1 of 13
No
No
Atha\AT3G15220
1 of 13
No
No
Atha\AT3G19300
1 of 13
No
No
Atha\AT3G24715
1 of 13
No
No
Atha\AT3G46920
1 of 13
No
No
Atha\AT3G46930
1 of 13
No
No
Atha\AT4G23050
1 of 13
No
No
Atha\AT4G24480
1 of 13
No
No
Atha\AT5G11850
1 of 13
No
No
Atha\AT5G35580
1 of 13
No
No
Atha\AT5G48740
1 of 13
No
Yes
Atha\AT5G49470
1 of 13
No
No
Atha\AT5G57610
1 of 13
No
No
Atha\AT5G58950
1 of 13
No
No
Atha\ATMAP4K ALPHA1
1 of 13
No
No
Atha\ATMRK1
1 of 13
No
No
Atha\ATN1
1 of 13
No
No
Atha\CIPK7
1 of 13
No
No
Atha\CIPK12
1 of 13
No
No
Atha\CPK17
1 of 13
No
No
Atha\CPK21
1 of 13
No
No
Atha\CTEXP
1 of 13
No
No
Atha\CTR1
1 of 13
No
No
Atha\EDR1
1 of 13
No
No
Atha\HT1
1 of 13
No
No
Atha\MAP3KA
1 of 13
No
No
Atha\MAPKKK5
1 of 13
No
No
Atha\MAPKKK6
1 of 13
No
No
Atha\MAPKKK7
1 of 13
No
No
Atha\MAPKKK9
1 of 13
No
No
Atha\MEKK1
1 of 13
No
No
Atha\MEKK3
1 of 13
No
No
Atha\MKK2
1 of 13
No
No
Atha\MKK4
1 of 13
No
No
Atha\NEK1
1 of 13
No
No
Atha\NEK3
1 of 13
No
No
Atha\NEK4
1 of 13
No
No
Atha\NEK5
1 of 13
No
No
Atha\NEK6
1 of 13
No
No
Atha\Nek7
1 of 13
No
No
Atha\NP1
1 of 13
No
No
Atha\NP2
1 of 13
No
No
Atha\NP3
1 of 13
No
No
Atha\SIK1
1 of 13
No
No
Atha\SIP4
1 of 13
No
No
Atha\SNRK2.9
1 of 13
No
No
Atha\STY8
1 of 13
No
No
Atha\VIK
1 of 13
No
No
Atha\WRKY19
1 of 13
No
No
Atha\YDA
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\BCK1
1 of 13
Yes
No
Scer\CEX1
1 of 13
Yes
No
Scer\KIC1
1 of 13
Yes
No
Scer\PKH1
1 of 13
Yes
No
Scer\SCY1
1 of 13
Yes
No
Scer\STE20
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (7)
Spom\ark1
1 of 12
Yes
No
Spom\cdc7
1 of 12
Yes
Yes
Spom\fin1
1 of 12
Yes
No
Spom\mkh1
1 of 12
Yes
No
Spom\ppk3
1 of 12
Yes
No
Spom\ppk32
1 of 12
Yes
No
Spom\wis4
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Wnk. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (18)
Madm
4 of 13
Ask1
1 of 13
Btk
1 of 13
CG1344
1 of 13
CG1951
1 of 13
fray
1 of 13
InR
1 of 13
mbt
1 of 13
msn
1 of 13
Nek2
1 of 13
Pak3
1 of 13
Pak
1 of 13
slpr
1 of 13
Snrk
1 of 13
Takl1
1 of 13
Tao
1 of 13
wnd
1 of 13
yata
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 3 )
Potential Models Based on Orthology ( 2 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
WnkKK102654
ameliorates  autosomal dominant cerebellar ataxia
modeled by Hsap\ATXN182Q.UAS
(Park et al., 2013)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
WNK2; WNK lysine deficient protein kinase 2
7 of 14
606249
       
      WNK3; WNK lysine deficient protein kinase 3
      7 of 14
      300358
       
      WNK1; WNK lysine deficient protein kinase 1
      6 of 14
      PROTEIN KINASE, LYSINE-DEFICIENT 1; WNK1
       
      WNK4; WNK lysine deficient protein kinase 4
      6 of 14
      601844
        NRBP1; nuclear receptor binding protein 1
        3 of 14
        606010
             
            NRBP2; nuclear receptor binding protein 2
            3 of 14
            615563
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Dmel gene
                Ortholog showing functional complementation
                Supporting References
                Wnk
                Hsap\WNK2
                (Yarikipati et al., 2023)
                Interactions
                Summary of Physical Interactions
                Summary of Genetic Interactions
                Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please look at the allele data for full details of the genetic interactions
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Wnk
                suppressible
                Awh
                (Sato and Shibuya, 2013)
                Wnk
                suppressible
                axed
                (Izadifar et al., 2021)
                Wnk
                suppressible
                fray
                (Yarikipati et al., 2023, Wu et al., 2014, Sato and Shibuya, 2013)
                Wnk
                suppressible
                Ncc69
                (Wu et al., 2014)
                Wnk
                suppressible
                Nmnat
                (Izadifar et al., 2021)
                Wnk
                suppressible
                Sarm
                (Izadifar et al., 2021)
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                axed
                suppressible
                Wnk
                (Izadifar et al., 2021)
                dsh
                suppressible
                Wnk
                (Serysheva et al., 2013)
                fz2
                enhanceable
                Wnk
                (Serysheva et al., 2013)
                fz2
                suppressible
                Wnk
                (Serysheva et al., 2013)
                Ncc69
                suppressible
                Wnk
                (Schellinger et al., 2022)
                Nmnat
                suppressible
                Wnk
                (Izadifar et al., 2021)
                Sarm
                suppressible
                Wnk
                (Izadifar et al., 2021)
                External Data
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (genetic) - An integrated Molecular Interaction Database
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Linkouts
                SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                3L
                Recombination map
                3-47
                Cytogenetic map
                78D5-78D6
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                78D5-78D6
                Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                Experimentally Determined Recombination Data
                Location

                3-47

                (FlyBase, 2016)

                3-43.8

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (15)
                Genomic Clones (19)
                cDNA Clones (22)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   

                  polyclonal

                  (Izadifar et al., 2021)
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

                      (Boutros et al., 2004)

                      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                      (Kiger et al., 2003)
                      Relationship to Other Genes
                      Source for database merge of
                      Additional comments
                      Nomenclature History
                      Source for database identify of

                      Source for identity of: Wnk CG7177

                      (Berger et al., 2008)
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (14)
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 46 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      FlyMine - An integrated database for Drosophila genomics
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      MIST (genetic) - An integrated Molecular Interaction Database
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                      References (91)