FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Wnk, fray
FlyBase ID
FBig0000098259
Interaction Network
Interactions Browser links
Wnk network
fray network
Reported Interactions
FBrf0220694-1.coIP.WB
Reference
Sato and Shibuya, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

DWNK

bait

HA tag

fray 

Fray

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sato and Shibuya, 2013)
FBrf0220694-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

DWNK

enzyme

FLAG tag

Wnk 

DWNK

enzyme

FLAG tag

Wnk 
fray 

Fray

enzyme target

GST tag, [32]P label

fray 

Fray

enzyme target

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0222370-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

Wnk

enzyme

GST tag

Wnk 

Wnk

enzyme

GST tag

Wnk 
Wnk 
fray 

Fray

enzyme target

GST tag, [32]P label

fray 

Fray

enzyme target

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0257908-1.coIP.WB
Reference
Yarikipati et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Wnk 

Wnk

bait

Myc tag

Wnk 
fray 

Fray

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Wnk 
RFSV motif residues 1804 and 1805
necessary binding region
aa 1804-1805

RF1804-1805AA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yarikipati et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)