FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Wnk
FlyBase ID
FBig0000098095
Interaction Network
Interactions Browser links
Wnk network
Reported Interactions
FBrf0220694-2.ENZ.A
Reference
Sato and Shibuya, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

DWNK

self

FLAG tag, [32]P label

Wnk 

DWNK

self

FLAG tag, [32]P label

Wnk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Wnk 
active site
mutation disrupting interaction
aa 420

D420A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme derived from HEK 293T cell extract.

(Sato and Shibuya, 2013)

Wnk is autophosphorylated.

(Sato and Shibuya, 2013)
FBrf0222370-1.ENZ.A
Reference
Serysheva et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

Wnk

self

GST tag, [32]P label

Wnk 

Wnk

self

GST tag, [32]P label

Wnk 
Wnk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Wnk 
kinase domain
mutation disrupting interaction
aa 420

D420A, kinase inactive

Wnk 
kinase domain
mutation increasing interaction
aa 382

S382E, constitutively active

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system.

(Serysheva et al., 2013)

Wnk is autophosphorylated.

(Serysheva et al., 2013)
FBrf0238827-1.ENZ.MS
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

DmWNK

enzyme

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0248803-1.ENZ.MS
Reference
Pleinis et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnk 

WNK

self

His tag

Wnk 

WNK

self

His tag

Wnk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Wnk 
kinase domain
sufficient binding region
aa 261-534
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system.

(Pleinis et al., 2021)

Autophosphorylation at Ser434 measured by mass spectrometry decreased with increasing K+ concentration.

(Pleinis et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (3)