FlyBase Allele Report: Dmel\Wnk[D420A.UAS.cWa]

General Information

Symbol

Dmel\Wnk^{D420A.UAS.cWa}

Species

D. melanogaster

Name

FlyBase ID

FBal0300920

Feature type

allele

Associated gene

Dmel\Wnk

Associated Insertion(s)

Carried in Construct

M{UAS-Wnk.D420A}

Also Known As

UAS-WNK^D420A

Key Links

Transgenic product class

dominant_negative_variant

(Wu et al., 2014)

missense_variant

(Wu et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Wu et al., 2014)

missense_variant

(Wu et al., 2014)

Mutagen

in vitro construct

(Wu et al., 2014)

Progenitor genotype

Carried in construct

M{UAS-Wnk.D420A}

Cytology

Description

UAS regulatory sequences drive expression of a dominant negative mutant Wnk polypeptide carrying amino acid substitution D420A.

(Wu et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Wnk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of pseudohypoaldosteronism

CEA

(Wu et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage, with Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

Phenotype Manifest In

adult Malpighian tubule, with Scer\GAL4^c42

(Wu et al., 2014)

wing blade, with Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

Detailed Description

Statement

Reference

Expression of Scer\GAL4^c42>Wnk^{D420A.Scer\UAS.cWa} decreases K[+] flux in Malpighian tubules.

(Wu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa} has decreased size | adult stage phenotype, non-enhanceable by kcc^KK107965/Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa} has decreased size | adult stage phenotype, non-suppressible by kcc^KK107965/Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa} has wing blade phenotype, non-enhanceable by kcc^KK107965/Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Wnk^{D420A.UAS.cWa} has wing blade phenotype, non-suppressible by kcc^KK107965/Scer\GAL4^Bx-MS1096

(Yarikipati et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Wnk^{D420A.Scer\UAS.cWa}

Wnk^{D420A.UAS.cWa}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close